JPH11515095A - 動物及び植物の細胞を検出するための染色剤及び毛管スライド - Google Patents
動物及び植物の細胞を検出するための染色剤及び毛管スライドInfo
- Publication number
- JPH11515095A JPH11515095A JP9510980A JP51098097A JPH11515095A JP H11515095 A JPH11515095 A JP H11515095A JP 9510980 A JP9510980 A JP 9510980A JP 51098097 A JP51098097 A JP 51098097A JP H11515095 A JPH11515095 A JP H11515095A
- Authority
- JP
- Japan
- Prior art keywords
- slide
- blood
- base
- cover
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 10
- 210000004369 blood Anatomy 0.000 claims abstract description 42
- 239000008280 blood Substances 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000007853 buffer solution Substances 0.000 claims abstract description 11
- 230000009471 action Effects 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 239000002904 solvent Substances 0.000 claims abstract description 8
- 230000002378 acidificating effect Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 19
- 238000010186 staining Methods 0.000 claims description 14
- 210000004027 cell Anatomy 0.000 claims description 11
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical group O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 210000003855 cell nucleus Anatomy 0.000 claims description 5
- 230000001086 cytosolic effect Effects 0.000 claims description 5
- 239000011859 microparticle Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 230000003204 osmotic effect Effects 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000004043 dyeing Methods 0.000 claims description 4
- 239000003607 modifier Substances 0.000 claims description 4
- 238000007447 staining method Methods 0.000 claims description 4
- KFZNPGQYVZZSNV-UHFFFAOYSA-M azure B Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(NC)=CC=C3N=C21 KFZNPGQYVZZSNV-UHFFFAOYSA-M 0.000 claims description 3
- 108091092356 cellular DNA Proteins 0.000 claims description 3
- 108091092328 cellular RNA Proteins 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 210000001519 tissue Anatomy 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 239000005357 flat glass Substances 0.000 claims description 2
- 239000005329 float glass Substances 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 239000000565 sealant Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000000975 dye Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 5
- 210000000601 blood cell Anatomy 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 125000006850 spacer group Chemical group 0.000 description 3
- 229940093475 2-ethoxyethanol Drugs 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 101000640899 Homo sapiens Solute carrier family 12 member 2 Proteins 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000010137 moulding (plastic) Methods 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9518129.3 | 1995-09-06 | ||
| GBGB9518129.3A GB9518129D0 (en) | 1995-09-06 | 1995-09-06 | Stain and capillary slide |
| PCT/GB1996/002192 WO1997009616A1 (en) | 1995-09-06 | 1996-09-05 | Stain and capillary slide to detect animal and plant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11515095A true JPH11515095A (ja) | 1999-12-21 |
Family
ID=10780244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9510980A Pending JPH11515095A (ja) | 1995-09-06 | 1996-09-05 | 動物及び植物の細胞を検出するための染色剤及び毛管スライド |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0848818A1 (de) |
| JP (1) | JPH11515095A (de) |
| AU (1) | AU6884196A (de) |
| CA (1) | CA2230889A1 (de) |
| GB (1) | GB9518129D0 (de) |
| WO (1) | WO1997009616A1 (de) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014002046A (ja) * | 2012-06-19 | 2014-01-09 | Murazumi Kogyo Kk | 液状試料検査用プレパラート |
| CN103827654A (zh) * | 2011-09-13 | 2014-05-28 | 皇家飞利浦有限公司 | 用于制备进行检查的细胞学样品的系统和试剂盒 |
| US9176121B2 (en) | 2004-02-13 | 2015-11-03 | Roche Diagnostics Hematology, Inc. | Identification of blood elements using inverted microscopy |
| JP2019101021A (ja) * | 2017-11-28 | 2019-06-24 | 東ソー株式会社 | 生体物質保持装置、および生体物質の検出方法 |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687023B (zh) | 2009-11-13 | 2016-01-06 | 文塔纳医疗系统公司 | 用于可调容纳体积的薄膜处理装置 |
| US10746752B2 (en) | 2009-11-13 | 2020-08-18 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| US9498791B2 (en) | 2009-11-13 | 2016-11-22 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| USD728120S1 (en) | 2013-03-15 | 2015-04-28 | Ventana Medical Systems, Inc. | Arcuate member for moving liquids along a microscope slide |
-
1995
- 1995-09-06 GB GBGB9518129.3A patent/GB9518129D0/en active Pending
-
1996
- 1996-09-05 WO PCT/GB1996/002192 patent/WO1997009616A1/en not_active Application Discontinuation
- 1996-09-05 CA CA 2230889 patent/CA2230889A1/en not_active Abandoned
- 1996-09-05 EP EP96929428A patent/EP0848818A1/de not_active Withdrawn
- 1996-09-05 JP JP9510980A patent/JPH11515095A/ja active Pending
- 1996-09-05 AU AU68841/96A patent/AU6884196A/en not_active Abandoned
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9176121B2 (en) | 2004-02-13 | 2015-11-03 | Roche Diagnostics Hematology, Inc. | Identification of blood elements using inverted microscopy |
| CN103827654A (zh) * | 2011-09-13 | 2014-05-28 | 皇家飞利浦有限公司 | 用于制备进行检查的细胞学样品的系统和试剂盒 |
| JP2014002046A (ja) * | 2012-06-19 | 2014-01-09 | Murazumi Kogyo Kk | 液状試料検査用プレパラート |
| JP2019101021A (ja) * | 2017-11-28 | 2019-06-24 | 東ソー株式会社 | 生体物質保持装置、および生体物質の検出方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997009616A1 (en) | 1997-03-13 |
| AU6884196A (en) | 1997-03-27 |
| GB9518129D0 (en) | 1995-11-08 |
| EP0848818A1 (de) | 1998-06-24 |
| CA2230889A1 (en) | 1997-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100860653B1 (ko) | 세포학적 및 조직학적 고정제 조성물 및 이용 방법 | |
| DE69516401T3 (de) | Verfahren und vorrichtung zum nachweis spezifischer zielzellen in spezialisierten und gemischten zellpopulationen und in lösungen, die gemischte zellpopulationen enthalten | |
| US4857300A (en) | Cytological and histological fixative formulation and methods for using same | |
| CN103201057B (zh) | 免疫学测定用蓝色金纳米颗粒、其制造方法以及使用该蓝色金纳米颗粒的测定方法 | |
| CA1124623A (en) | Polymer carrier and method for carrying out scientific, analytic and diagnostic examinations | |
| Tooze et al. | The occurrence and possible significance of haemoglobin in the chromosomal regions of mature erythrocyte nuclei of the newt Triturus cristatus cristatus | |
| JP2000509146A (ja) | 遅延クエンチングによりホルムアルデヒド固定を制御する方法および組成物 | |
| RU2619784C2 (ru) | Система и набор для получения цитологических образцов для исследования | |
| JPH0560759A (ja) | スクシンイミド含有ポリマー由来の生物学的活性試薬の製造方法及びそれを含んでなる分析要素並びにその用途 | |
| US8163565B2 (en) | Light curing fixative | |
| CN107167356B (zh) | 一种尿液中细胞染色后快速显色的染色剂及其使用方法 | |
| JPH11515095A (ja) | 動物及び植物の細胞を検出するための染色剤及び毛管スライド | |
| JP2001509591A (ja) | 細胞ｄｎａを染色するための組成物および方法 | |
| JP2001512669A (ja) | 液体試料から粒子状物質を分離するための方法及び装置 | |
| CN115372107A (zh) | 前处理试剂及制备方法和细胞染色方法及前处理方法 | |
| US3961039A (en) | Urinary sediment stain | |
| Eskelund | Determination of genetic sex by examination of epithelial cells in urine | |
| Horowitz et al. | Analysis of glycerol-3H transport in the frog oocyte by extractive and radioautographic techniques | |
| Gao et al. | A new method for transfer of polyethylene glycol-embedded tissue sections to silanated slides for immunocytochemistry. | |
| DE2023989B2 (de) | Verfahren zur Herstellung von aus Antigen Tragerstoffgemischen bestehenden und gegebenenfalls Antikörper enthalten den fertigen serodiagnostischen, auf einen Objektträger aufgebrachten lager bestandigen Testsubstanzen | |
| CN111100839B (zh) | EGFR/Vimentin/叶酸免疫脂质体磁球、制备方法及试剂盒 | |
| CA1313112C (en) | Analytical method and element for ferrous ion assay | |
| Wallace et al. | A sensitive microassay for protein in cells cultured on collagen | |
| Fukuda et al. | Combined protein and DNA measurements by the ninhydrin-Schiff and Feulgen techniques | |
| DE3811692C2 (de) |