CA2230889A1 - Stain and capillary slide to detect animal and plant cells - Google Patents
Stain and capillary slide to detect animal and plant cells Download PDFInfo
- Publication number
- CA2230889A1 CA2230889A1 CA 2230889 CA2230889A CA2230889A1 CA 2230889 A1 CA2230889 A1 CA 2230889A1 CA 2230889 CA2230889 CA 2230889 CA 2230889 A CA2230889 A CA 2230889A CA 2230889 A1 CA2230889 A1 CA 2230889A1
- Authority
- CA
- Canada
- Prior art keywords
- slide
- stain
- blood
- base
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 13
- 210000004369 blood Anatomy 0.000 claims abstract description 41
- 239000008280 blood Substances 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000000872 buffer Substances 0.000 claims abstract description 10
- 238000010186 staining Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 239000003607 modifier Substances 0.000 claims abstract description 7
- 230000002378 acidificating effect Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 21
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 239000004005 microsphere Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000001086 cytosolic effect Effects 0.000 claims description 5
- 239000000834 fixative Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 229940093475 2-ethoxyethanol Drugs 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 108091092328 cellular RNA Proteins 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 239000000565 sealant Substances 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 2
- KFZNPGQYVZZSNV-UHFFFAOYSA-M azure B Chemical class [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(NC)=CC=C3N=C21 KFZNPGQYVZZSNV-UHFFFAOYSA-M 0.000 claims description 2
- 108091092356 cellular DNA Proteins 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 239000005357 flat glass Substances 0.000 claims description 2
- 239000005329 float glass Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 10
- 125000006850 spacer group Chemical group 0.000 description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical group [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000004683 dihydrates Chemical class 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9518129.3A GB9518129D0 (en) | 1995-09-06 | 1995-09-06 | Stain and capillary slide |
| GB9518129.3 | 1995-09-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2230889A1 true CA2230889A1 (en) | 1997-03-13 |
Family
ID=10780244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2230889 Abandoned CA2230889A1 (en) | 1995-09-06 | 1996-09-05 | Stain and capillary slide to detect animal and plant cells |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0848818A1 (de) |
| JP (1) | JPH11515095A (de) |
| AU (1) | AU6884196A (de) |
| CA (1) | CA2230889A1 (de) |
| GB (1) | GB9518129D0 (de) |
| WO (1) | WO1997009616A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9176121B2 (en) | 2004-02-13 | 2015-11-03 | Roche Diagnostics Hematology, Inc. | Identification of blood elements using inverted microscopy |
| US8911815B2 (en) | 2009-11-13 | 2014-12-16 | Ventana Medical Systems, Inc. | Thin film processing apparatuses for adjustable volume accommodation |
| US10746752B2 (en) | 2009-11-13 | 2020-08-18 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| US9498791B2 (en) | 2009-11-13 | 2016-11-22 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| MX2014002844A (es) * | 2011-09-13 | 2014-07-09 | Koninkl Philips Nv | Sistema y kit para preparar una muestra citologica para examen. |
| JP2014002046A (ja) * | 2012-06-19 | 2014-01-09 | Murazumi Kogyo Kk | 液状試料検査用プレパラート |
| USD728120S1 (en) | 2013-03-15 | 2015-04-28 | Ventana Medical Systems, Inc. | Arcuate member for moving liquids along a microscope slide |
| JP2019101021A (ja) * | 2017-11-28 | 2019-06-24 | 東ソー株式会社 | 生体物質保持装置、および生体物質の検出方法 |
-
1995
- 1995-09-06 GB GBGB9518129.3A patent/GB9518129D0/en active Pending
-
1996
- 1996-09-05 WO PCT/GB1996/002192 patent/WO1997009616A1/en not_active Application Discontinuation
- 1996-09-05 JP JP9510980A patent/JPH11515095A/ja active Pending
- 1996-09-05 AU AU68841/96A patent/AU6884196A/en not_active Abandoned
- 1996-09-05 CA CA 2230889 patent/CA2230889A1/en not_active Abandoned
- 1996-09-05 EP EP96929428A patent/EP0848818A1/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| GB9518129D0 (en) | 1995-11-08 |
| WO1997009616A1 (en) | 1997-03-13 |
| EP0848818A1 (de) | 1998-06-24 |
| AU6884196A (en) | 1997-03-27 |
| JPH11515095A (ja) | 1999-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |