CA2230889A1 - Matiere colorante et lame capillaire de detection de cellules animales et vegetales - Google Patents
Matiere colorante et lame capillaire de detection de cellules animales et vegetales Download PDFInfo
- Publication number
- CA2230889A1 CA2230889A1 CA 2230889 CA2230889A CA2230889A1 CA 2230889 A1 CA2230889 A1 CA 2230889A1 CA 2230889 CA2230889 CA 2230889 CA 2230889 A CA2230889 A CA 2230889A CA 2230889 A1 CA2230889 A1 CA 2230889A1
- Authority
- CA
- Canada
- Prior art keywords
- slide
- stain
- blood
- base
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 13
- 210000004369 blood Anatomy 0.000 claims abstract description 41
- 239000008280 blood Substances 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000000872 buffer Substances 0.000 claims abstract description 10
- 238000010186 staining Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 239000003607 modifier Substances 0.000 claims abstract description 7
- 230000002378 acidificating effect Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 21
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 239000004005 microsphere Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000001086 cytosolic effect Effects 0.000 claims description 5
- 239000000834 fixative Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 229940093475 2-ethoxyethanol Drugs 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 108091092328 cellular RNA Proteins 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 239000000565 sealant Substances 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 2
- KFZNPGQYVZZSNV-UHFFFAOYSA-M azure B Chemical class [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(NC)=CC=C3N=C21 KFZNPGQYVZZSNV-UHFFFAOYSA-M 0.000 claims description 2
- 108091092356 cellular DNA Proteins 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 239000005357 flat glass Substances 0.000 claims description 2
- 239000005329 float glass Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 10
- 125000006850 spacer group Chemical group 0.000 description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical group [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 229940038773 trisodium citrate Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000004683 dihydrates Chemical class 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
L'invention concerne une matière colorante présentant un tampon acide ou basique, un modificateur de membrane, un composé colorant et un solvant, la matière colorante étant adaptée à une utilisation dans la préparation d'échantillons de sang ou autres prélevés sur des animaux ou des plantes. La matière colorante peut être utilisée en liaison avec une lame, laquelle est décrite comme étant adaptée pour prélever un liquide dans elle-même par capillarité.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9518129.3A GB9518129D0 (en) | 1995-09-06 | 1995-09-06 | Stain and capillary slide |
| GB9518129.3 | 1995-09-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2230889A1 true CA2230889A1 (fr) | 1997-03-13 |
Family
ID=10780244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2230889 Abandoned CA2230889A1 (fr) | 1995-09-06 | 1996-09-05 | Matiere colorante et lame capillaire de detection de cellules animales et vegetales |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0848818A1 (fr) |
| JP (1) | JPH11515095A (fr) |
| AU (1) | AU6884196A (fr) |
| CA (1) | CA2230889A1 (fr) |
| GB (1) | GB9518129D0 (fr) |
| WO (1) | WO1997009616A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9176121B2 (en) | 2004-02-13 | 2015-11-03 | Roche Diagnostics Hematology, Inc. | Identification of blood elements using inverted microscopy |
| US10746752B2 (en) | 2009-11-13 | 2020-08-18 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| CA2778837C (fr) | 2009-11-13 | 2016-01-19 | Ventana Medical Systems, Inc. | Appareils de traitement a couches minces pour adaptation reglable de volume |
| US9498791B2 (en) | 2009-11-13 | 2016-11-22 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
| WO2013038306A1 (fr) * | 2011-09-13 | 2013-03-21 | Koninklijke Philips Electronics N.V. | Système et trousse pour la préparation d'un échantillon cytologique pour l'examen |
| JP2014002046A (ja) * | 2012-06-19 | 2014-01-09 | Murazumi Kogyo Kk | 液状試料検査用プレパラート |
| USD728120S1 (en) | 2013-03-15 | 2015-04-28 | Ventana Medical Systems, Inc. | Arcuate member for moving liquids along a microscope slide |
| JP2019101021A (ja) * | 2017-11-28 | 2019-06-24 | 東ソー株式会社 | 生体物質保持装置、および生体物質の検出方法 |
-
1995
- 1995-09-06 GB GBGB9518129.3A patent/GB9518129D0/en active Pending
-
1996
- 1996-09-05 AU AU68841/96A patent/AU6884196A/en not_active Abandoned
- 1996-09-05 CA CA 2230889 patent/CA2230889A1/fr not_active Abandoned
- 1996-09-05 JP JP9510980A patent/JPH11515095A/ja active Pending
- 1996-09-05 EP EP96929428A patent/EP0848818A1/fr not_active Withdrawn
- 1996-09-05 WO PCT/GB1996/002192 patent/WO1997009616A1/fr not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11515095A (ja) | 1999-12-21 |
| AU6884196A (en) | 1997-03-27 |
| WO1997009616A1 (fr) | 1997-03-13 |
| GB9518129D0 (en) | 1995-11-08 |
| EP0848818A1 (fr) | 1998-06-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |