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• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
  • C12N9/14—Hydrolases (3)
  • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
  • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
  • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
  • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
  • C12N9/6424—Serine endopeptidases (3.4.21)
  • C12N9/644—Coagulation factor IXa (3.4.21.22)
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • A61K38/36—Blood coagulation or fibrinolysis factors
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • A61K38/36—Blood coagulation or fibrinolysis factors
  • A61K38/37—Factors VIII
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • A61K38/38—Albumins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
  • C07K1/14—Extraction; Separation; Purification
  • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
  • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
  • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
  • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
  • C07K14/4717—Plasma globulins, lactoglobulin
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/745—Blood coagulation or fibrinolysis factors
  • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/76—Albumins
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/81—Protease inhibitors
  • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
  • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
  • C07K14/8121—Serpins
  • C07K14/8128—Antithrombin III
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
  • C12N7/02—Recovery or purification
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Y—ENZYMES
  • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
  • C12Y304/21—Serine endopeptidases (3.4.21)
  • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Definitions

• the present invention relates to a method of virus-filtering a solution that contains at least one macromolecule, by virtue of the total salt content of the solution lying in the range of from about 0.2 M up to saturation of the solution with the salt concerned.
• the inventive method reduces the residence time and the extent to which the solution need to be diluted, and optimizes the yield when virus-filtering primarily proteins, polysaccharides and polypeptides.
• the reduction in virus content is at least as good as with conventional techniques where the total salt content is low.
• the present invention facilitates virus filtration with the aid of the so-called "dead-end" technique, which affords several process and economic advantages in comparison with the tangential virus-filtering technique normally used.
• the yield obtained in the virus-filtering stage is increased from about 70% to above 95%, by raising the salt content of the solution in accordance with the present invention.
• viruses are inactivated with the aid of chemical additives, primarily solvents and detergents, and /or by exposing the viruses to elevated temperatures.
• the former method has the drawback of functioning solely on virus with lipid envelopes, for instance hepatitis virus B and HTV.
• the latter technique mentioned above has the drawback that many proteins are thermally unstable at those temperatures required to effectively reduce the contaminating virus.
• US-A-4,473,494 (assigned to the U.S.
• EP-A-0307373 (assigned to Ares-Serono) relates to removal of viruses and/or other contaminants from biological materials in fluid form by using ultrafiltration membranes having a 100,000 Da cut-off.
• a preferred biological material is human growth hormone.
• the total content of salt in the virus-filtering step lies in the range of from 0.01 up to 0.10 M (NH 4 C0 3 ), i.e. the total content of salt is conventional.
• One object of the present invention is to markedly reduce the residence time when virus- filtering solutions that contain macromolecules.
• Another object of the present invention is to markedly reduce the liquid volumes when virus-filtering solutions that contain macromolecules.
• a further object of the present invention is to reduce the filter area required to effectively virus-filter solutions that contain macromolecules.
• Yet another object of the present invention is to achieve a macromolecule yield in excess of about 90% in the virus-filtering stage.
• Still another object of the present invention is to reduce the polymerization obtained on the virus filter surface, so as to enable the rate of flow to be increased and the process time to be decreased.
• virus filtration can be effected much more effectively than previously known, by increasing the salt content of the solution. This discovery is surprising, because hitherto in virus filtration of proteins it has been believed that solely the protein concentration, the rate of flow and the pH have had any influence on the process.
• the effectiveness of the present invention is also enhanced when the difference in the size and/or the molecular weight of the contaminants and the product increases, i.e. with increasing concentrations of high molecular contaminants in the product.
• the present invention also facilitates specific fractions to be separated from a desired product, for instance enables undesirable proteins to be separated from the protein that constitutes the product.
• the use of a high salt content according to the present invention also enables the use of the so-called "dead-end” filtering technique.
• This preferred embodiment has several advantages over conventional tangential filtering processes normally applied, especially with a pore size of about 5-30 nm. For instance, the equipment and operating procedures required are much simpler and therewith less expensive.
• the use of "dead-end” filtration also reduces the loss of the macromolecule, reduces the process time, increases the permeability of the macromolecule through the filter, and also enables a generally constant concentration of the macromolecule to be achieved over the filter as well as a constant membrane pressure.
• Another advantage with the dead-end filtering technique is the fact that scaling-up of virus filtering processes from laboratory to industrial scale is considerably facilitated.
• the total salt content of the solution suitably lies within the range of from 0.3 up to 3.0 M, preferably within the range of from 0.4 up to 2.5 M, and more preferably within the range of from 0.6 up to 2.0 M. It is particularly preferred that the total salt content of the solution lies within the range of from 0.8 up to 1.5 M.
• the total salt content of the solution can be adjusted by adding any acceptable salt.
• any acceptable salt for instance, it is possible to use soluble inorganic salts, soluble organic salts or combinations of such salts. It is assumed that important process advantages are obtained when using salts which exhibit a high salting-out effect in accordance with the so-called Hofmeister series. Reference is here made to S. Glasstone, Textbook of Physical
• anions which have such high salting-out effect are citrate, tartrate, sulfate, acetate and phosphate.
• Cations that can be used advantageously when practicing the present invention are monovalent cations, such as sodium, potassium and ammonium, as well as divalent cations, such as calcium.
• Sodium chloride, potassium chloride, sodium acetate and sodium citrate or combinations thereof are particularly preferred salts in accordance with the invention, in view of the advantages that are afforded by pharmaceutically acceptable additives. It is also conceivable to add one or more salts in sequence, when the filtration process is carried out in two or more steps.
• a protein concentration within the range of from about 5 up to about 10 mg/ml solution is often recommended for virus filtration.
• solutions having a higher protein concentration from about 10 up t about 20 mg/ml, could be processed advantageously through the virus filter.
• the solution should have a temperature within the range from 0°C up to the temperature at which the protein concerned is denatured.
• the temperature of the solution suitably lies within the range of from 10°C up to 50°C, preferably from 20°C up to 35°C.
• the solution should have a pH in the range of from about 3 up to about 9, suitably from 4 up to 8.
• the pH of the protein solution should not lie too close to the isoelectric point of the protein concerned. For instance, in the case of gammaglobulin, a better result is obtained with a pH of 5.5 than with a pH of 6.8.
• solution refers to a solution that contains at least 50 percent by weight of water, optionally including one or more solvents, such as methanol, ethanol, acetone or acetonirrile.
• the present invention can be used to optimize process procedures when virus-filtering solutions that contain a large number of different types of macromolecules.
• macromolecules examples of such molecules are proteins, polysaccharides and polypeptides or combinations thereof.
• the origin of the macromolecules is irrelevant to the use of the present invention.
• the macromolecules may thus derive from the plant kingdom or the animal kingdom or may be produced initially by industrial processes. However, the macromolecules are suitably of human or animal origin or engineered genetically (recombinants).
• Particularly appropriate proteins in regard of the present invention are factor VHI, factor DC, antithrombin HI, gammaglobulin, albumin, streptokinase, apolipoproteins and growth hormones.
• a particularly preferred factor LX product is Nanotiv®, which is supplied by Pharmacia AB, Sweden.
• the advantage with this product is that its specific activity prior to filtration is sufficiently high to enable the use of a filter of very fine structure. This enables the virus concentration to be lowered to an extremely low level, at the same time as the filtering process itself is very rapid and produces a high yield.
• factor Vm are deletion derivatives of recombinant produced factor VDI products.
• a particularly preferred factor Vm product is r-VHI SQ supplied by Pharmacia AB, Sweden.
• One advantage with this product is that the recombinant produced product molecule lacks the inactive intermediate part of the natural factor VLII molecule. This gives the molecule a mean molecular weight of about 170,000. A molecule of this size is particularly suited for filtration with such filters as those which enable a considerable virus reduction to be achieved.
• Preferred apolipoproteins include apolipoprotein Al (Apo Al), apolipoprotein AH (Apo AH), apolipoprotein AIV (Apo A1V), apolipoprotein E (Apo E) and variants or mixtures thereof.
• Variants include preforms, fragments and truncated, extended or mutated forms of Apo Al, Apo II, Apo IV and Apo E. Mutated forms in which at least one arginine group has been replaced with a cystein group are particularly preferred.
• Apo A-LMilano is Apo A-LMilano (Apo A-IM), also produced with recombinant DNA technique by Pharmacia AB, Sweden.
• glycosaminoglycans and bacteria polysaccharides.
• glycos- aminoglycans are heparins, heparin fragments, heparin derivatives, heparan sulfate and hyaluronic acid.
• a particularly preferred group of glycosaminoglycans is comprised of low molecular weight heparins having a mean molecular weight of up to about 10,000, preferably from 2,000 up to 8,000.
• bioactive polypeptides such as recombinant human growth hormones produced in mammalian cells.
• the present invention can thus be used to optimize the process of virus-filtering solutions that contain, e.g., proteins, polysaccharides and polypeptides.
• virus-filtering solutions that contain, e.g., proteins, polysaccharides and polypeptides.
• the invention is described in the following with reference to solutions that contain proteins, more specifically proteins that occur naturally in the human organism.
• viruses that may be present in protein solutions will normally be much larger than the proteins themselves. It is thus presumable that viruses can be removed from proteins in accordance with size, for instance by filtration. Viruses that can be removed efficiently with the present invention, can have a size smaller than about 350 ran. The size of the viruses that can be removed, suitably is smaller than 200 run, preferably smaller than 150 ran. Normally, the viruses that can be removed are larger than about 20 ran, i.e. the approximate size of the parvo virus.
• the present invention is primarily intended for removing viruses from macromolecules, where the macromolecules are the product of interest. It is, however, within the scope of the invention, to use the present method for separating viruses from macromolecules, where the viruses are the product of interest.
• An example is the purification of parvovirus for use as a testing agent, and poliovirus for use a vaccine, wherein e.g. proteins and polysaccharides can be removed by the present method.
• Virus filtration is normally carried out in a tangential filtering process or in a so-called "dead-end" filtering process.
• tangential virus filtration the protein solution is pumped around at a constant rate of flow on the retention side, while another pump draws the protein solution through the filter by suction.
• a buffer is added on the retention side. This procedure is repeated a number of times, as necessary, with the major part of the remaining protein passing through the filter while retaining the virus on the retention side. Such a process is called diafiltration.
• the filter is normally discarded after each run, to avoid transferring the virus.
• dead-end virus filtration In the case of so-called "dead-end” virus filtration, the same virus filter as that used in tangential virus filtration can be used, although the peripheral equipment and operating procedures are much simpler and less expensive than in the case of tangential virus filtration.
• "dead-end” filtration involves placing the macromolecule- containing solution in a pressure vessel prior to filtration and pressing the solution through the virus filter with the aid of a pressure source, suitably nitrogen (gas).
• the degree of fineness of filters generally, is normally given as pore size or the approximate molecular weight (relative molecular mass) at which the molecules are stopped by the filter, the so called cut-off.
• the virus filters can have a cut-off of about 1,000,000, suitably 500,000.
• the virus filters should have a cut-off of 200,000, preferably 100,000.
• the virus filter should have a cut-off slightly higher than the macromolecule which is virus-filtered.
• Virus filters are known in the art and are supplied by Millipore from Massachusetts, USA and Asahi Chemical Industry Co., Ltd. from Japan, among others. Millipore supplies filters having two different types of membrane, depending on the size of the protein concerned. For instance, Millipore supplies, among others, ViresolveTM/70 for proteins having a molecular weight, or relative molecular mass, of up to about 70,000, and Vire- solveTM/180 for proteins having a molecular weight of up to about 180,000. This latter filter can be used for monoclonal antibodies, for instance. Asahi Chemical Industry supplies, among other things, PlanovaTM 35 and PlanovaTM 15 filters, this latter filter being used to remove smaller viruses, such as the polio virus.
• the choice of filter will depend on the size of the protein concerned, among other things.
• Factor LX, antithrombin HI, human serum albumin (HSA) and Apo A-LM (the dimer) all have a molecular weight of roughly 60,000-70,000, wherein ViresolveTM/70, for instance, is a suitable alternative.
• Gammaglobulin has a molecular weight of about 180,000, wherein ViresolveTM/180, for instance, is a suitable alternative.
• the latter filter is also suitable for use with the recombinant produced factor VLLI product, r-VLLI SQ, which has a molecular weight of about 170,000, as mentioned before.
• a fine structure filter also assumes that the protein solution has a high degree of purity prior to filtration.
• the use of a fine structure filter is a prerequisite for the ability to produce protein solutions which have a very low virus content in the end product.
• a filter of very fine structure for instance ViresolveTM/70.
• the virus concentration cannot be lowered to quite such a low level when using ViresolveTM/180.
• the effectiveness, or efficiency, of the filtering stage is influenced by the purity of the protein solution delivered to the filter.
• a high specific activity prior to filtration results in a higher yield in the filtering stage.
• the protein yield in the filtering stage can be increased from about 70% to above 95%.
• the present invention it is possible to reduce the content of very small non- enveloped viruses, such as the parvovirus, by at least 3 logs, suitably at least 4 logs, and preferably at least 5 logs.
• the reduction is very good with the tangential technique, but even better with the "dead-end" technique, when applied according to the present invention.
• virus filtration is preferably carried out at the end of a protein manufacturing sequence, since a high specific activity prior to filtration will result in a higher protein yield in the filtering stage.
• the present invention is preferably applied as a last purification stage, optionally followed by a stage for adjusting, for instance, protein concentration, salt content or the pH of the end product.
• a following diafiltration stage using a UF-membrane may also be applied to remove salts which although advantageous from a process or economic aspect during virus filtration should not be included in the end product.
• Protein solutions which are ready for administration will normally contain a physiological solution, for instance 0.15 M sodium chloride at a pH of 7, in combination with one or more stabilizers, such as saccharose or amino acids.
• the virus filtration process may also be carried out in two or more steps, with or without intermediate process steps.
• the present invention effectively reduces the content of virus with lipid envelopes and viruses without lipid envelopes.
• viruses without a lipid envelope are the hepatitis virus A, polio virus and parvo virus, which are relatively small viruses.
• viruses with a lipid envelope are the hepatitis virus B, the hepatitis virus C and the Human Immunodeficiency Virus (HIV).
• HIV Human Immunodeficiency Virus
• factor IX as the macromolecule, to illustrate the effect of two salt contents on the protein sieving characteristic, the diafiltration volume and the yield.
• the solution was ultra-filtered between each stage, except between the chemical virus inactivating stage and the affinity chromatographic stage.
• Buffer 0.144 M NaCl+0.0055 M sodium citrate.
• Total salt content about 0.15 M.
• Protein concentration 0.5-1.0 A M units.
• Virus separating filters ViresolveTM/70.
• Transmembrane pressure 0.2-0.3 bar.
• Diafiltration with a dilution of about 1 volume unit per volume unit of entering protein solution (1+1) resulted in a yield of about 90%.
• Example 2 The same conditions were applied as those applied in Example 1, with the exception that in this case the buffer comprised 1.0 M NaCl+0.01 M sodium citrate, which gave a total salt content of about 1.0 M.
• the virus removing effect achieved with the experiments disclosed in Examples 1 and 2 was determined by a virus study.
• the study was carried out on parvovirus, which are non-lipid-enveloped viruses and which have a size of 20-25 ran. In principle, experiments with such viruses fall into the "worst case" category since they are some of the smallest viruses known.
• the parvovirus was added to the solutions containing factor LX, with a salt content of 0.144 M NaCl+0.0055 M sodium citrate (experiment 1) and 1.0 M NaCl+0.01 M sodium ci ⁇ trate (experiment 2) respectively.
• the solutions were then virus-filtered in accordance with Examples 1 and 2.
• the solutions were analyzed with respect to the parvovirus both before and after virus filtration.
• Diafiltration with dilution of about 3 volume units per volume unit of entering protein solution (1+3) resulted in a yield of about 65%.
• the buffer comprised 0.5 M NaCl+0.01 M sodium citrate. Total salt content: about 0.5 M.
• the buffer comprised 1.0 M NaCl+0.01 M sodium citrate. Total salt content: about 1.0 M.
• the buffer comprised 1.5 M NaCl+0.01 M sodium citrate. Total salt content: about 1.5 M.
• the buffer comprised 0.5 M potassium dihydrophosphate. Total salt content: 0.5 M.
• the buffer comprised 0.5 M NaCl. Total salt content: 0.5 M.
• the buffer comprised 0.5 M barium chloride. Total salt content: 0.5 M.
• gammaglobulin as the macromolecule to show the effect of salt content on protein sieving coefficient, diafiltration volume and yield.
• the solution containing gammaglobulin was a commercial product obtained from blood plasma, Gammonativ®, supplied by Pharmacia AB, Sweden. Prior to filtration, the gammaglobulin solution had been purified by an intitial Cohn fractionation followed by a chromatographic stage.
• the experimental conditions applied were the same as those applied in Example 1, with the exception that the virus-removing filter was a ViresolveTM/180 filter, the pH of the solution was 6.8 and the protein concentration was 2.5-5.0 A 280 units.
• the buffer comprised 2.2% albumin+0.15 M NaCl+0.02 M NaAc+0.075 M glycine. Total salt content: 0.17 M.
• Example 13 Determining the protein sieving coefficient.
• Diafiltration with a dilution degree of about 1 volume unit per volume unit of entering protein solution (1+1) resulted in a yield of 90%.
• Example 8 The same conditions were applied as those applied in Example 8, with the exception that in this case the pH of the solution was 5.5.
• Example 10 The same conditions were applied as those applied in Example 10, with the exception that in this case the buffer comprised 2.2% albumin+1.0 M NaCl+0.02 M NaAc+0.075 M glycine. Total salt content: about 1.0 M.
• the experimental conditions applied were the same as those applied in Example 1, with the exception that the protein concentration was about 10 A m units.
• the buffer comprised 0.15 M NaCl+0.02 M NaAc, resulting in a total salt content of 0.17 M. TABLE 16 Dete ⁇ nining the protein sieving coefficient.
• Example 12 The same conditions were applied as those applied in Example 12, with the exception that in this case the buffer comprised 1.0 M NaCl+0.02 M NaAc, resulting in a total salt content of about 1.0 M.
• Amount of protein solution prior to virus filtration (g): 294 1124 Protein cone. (A ⁇ units): 0.66 1.0 Retention flowrate (1/h): 40 0 Filtrate flowrate buffer (ml/min): 24 28
• Virus filtration of factor IX using the "dead-end" technique means less dilution, shorter process times and results in a higher yield and protein permeability.
• Virus filtration of AT HI when applying the "dead-end" technique means less dilution, affords higher protein permeability and shorter process times.
• High salt content result in improved protein permeability with regard to AT HI.
• HSA Human Serum Albumin
• Virus filtration of HSA when applying the "dead-end" technique means less dilution, and results in a higher yield and higher protein permeability and shorter process times.
• Virus-filtration of gammaglobulin with the "dead-end" technique involves less dilution, and results in a higher yield and protein permeability and shorter process times.
• Virus separating filters ViresolveTM/70. Filtering technique: dead-end. Filter area: 10 ft 2 Retention flowrate: 01/h. Filtrate flowrate buffer: 201/h Transmembrane pressure: 0.3 bar.
• virus filtering antithrombin according to the invention can be applied on an industrial scale with excellent results.
• Example 15 The virus-removing effect achieved with the experiments disclosed in Example 15 was determined by a virus study, but at a higher salt content.
• the virus-filtering technique was the "dead-end" technique.
• the study was carried out on parvovirus, as in Example 3.
• the parvovirus was added to the solutions containing factor LX 1.0 M NaCl+0.01 M sodium citrate (experiment 1).
• the solutions were analyzed with respect to the parvovirus both before and after virus filtration.

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• 1995
  • 1995-02-24 SE SE9500724A patent/SE9500724D0/xx unknown
  • 1995-06-22 NZ NZ288789A patent/NZ288789A/en not_active IP Right Cessation
  • 1995-06-22 ES ES95923651T patent/ES2105992T3/es not_active Expired - Lifetime
  • 1995-06-22 MX MX9606639A patent/MX9606639A/es not_active IP Right Cessation
  • 1995-06-22 US US08/750,664 patent/US6486306B1/en not_active Expired - Fee Related
  • 1995-06-22 PT PT95923651T patent/PT796269E/pt unknown
  • 1995-06-22 JP JP50306296A patent/JP3676370B2/ja not_active Expired - Fee Related
  • 1995-06-22 AT AT95923651T patent/ATE212354T1/de not_active IP Right Cessation
  • 1995-06-22 DE DE69525176T patent/DE69525176T2/de not_active Revoked
  • 1995-06-22 EP EP95923651A patent/EP0796269B1/de not_active Revoked
  • 1995-06-22 CA CA002192683A patent/CA2192683C/en not_active Expired - Fee Related
  • 1995-06-22 WO PCT/SE1995/000777 patent/WO1996000237A1/en active IP Right Grant
  • 1995-06-22 DE DE0796269T patent/DE796269T1/de active Pending
  • 1995-06-22 DK DK95923651T patent/DK0796269T3/da active
• 1996
  • 1996-12-20 FI FI965145A patent/FI965145A0/fi not_active Application Discontinuation
  • 1996-12-20 NO NO19965523A patent/NO320952B1/no not_active IP Right Cessation
• 1997
  • 1997-11-28 GR GR970300038T patent/GR970300038T1/el unknown
• 2000
  • 2000-02-23 US US09/511,953 patent/US6399357B1/en not_active Expired - Fee Related
• 2002
  • 2002-11-26 US US10/304,902 patent/US20030191292A1/en not_active Abandoned
• 2005
  • 2005-02-23 US US11/064,770 patent/US20050203285A1/en not_active Abandoned

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