EP0654527B1 - Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen - Google Patents

Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen Download PDF

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Publication number
EP0654527B1
EP0654527B1 EP94203211A EP94203211A EP0654527B1 EP 0654527 B1 EP0654527 B1 EP 0654527B1 EP 94203211 A EP94203211 A EP 94203211A EP 94203211 A EP94203211 A EP 94203211A EP 0654527 B1 EP0654527 B1 EP 0654527B1
Authority
EP
European Patent Office
Prior art keywords
oil
enzymes
degumming
sludge
degummed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP94203211A
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German (de)
English (en)
French (fr)
Other versions
EP0654527A1 (de
Inventor
Henning Dr. Buchold
Rudolf Dr. Boensch
Joerg Dr. Schroeppel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GEA Group AG
Roehm GmbH Darmstadt
Original Assignee
Metallgesellschaft AG
Roehm GmbH Darmstadt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metallgesellschaft AG, Roehm GmbH Darmstadt filed Critical Metallgesellschaft AG
Publication of EP0654527A1 publication Critical patent/EP0654527A1/de
Application granted granted Critical
Publication of EP0654527B1 publication Critical patent/EP0654527B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead

Definitions

  • the invention relates to a method for degumming Vegetable oil, whereby the vegetable oil is adjusted to a pH of 3 to 6, an aqueous enzyme solution dispersed in the oil, which contains one of the enzymes phospholipase A1, A2 or B, which Enzymes in the oil with stirring at temperatures from 20 to 90 ° C in a degumming reactor can act and from the Degumming reactor withdrawn liquid degummed oil separates.
  • the invention is based, at the beginning mentioned processes the used enzymes in Degumming process at least partially to reuse. According to the invention, this is done in that the liquid withdrawn from the degumming reactor at temperatures of 20 to 90 ° C before or after the separation of the degummed oil a release agent or a Solubilizer admits and an aqueous, used enzyme containing, largely sludge-free solution that one at least partially leads back to the degumming reactor and dispersed in the oil to be degummed, the proportion used, recycled enzymes in total the enzyme dispersed in the oil is at least 10%. For cost reasons, it is advantageous if the share used, recycled enzymes in total of the enzymes dispersed in the oil at least 20% or more better, at least 50%.
  • a variant of the 2nd way is that of the Degumming reactor coming oily liquid admit a broker and then that separate degummed oil from the liquid, e.g. in a centrifuge. You get next to that degummed oil an aqueous, largely sludge-free Phase that contains the used enzymes. This The aqueous phase can be wholly or partly without further Treatment, before returning the degumming reactor, to mix them with the oil to be degummed.
  • solubilizer to be used or Release aids can vary widely, they is usually 0.1 to 100 g / l liquid. A Excess solubilizer or separation aid is not disturbing.
  • the vegetable oil to be completely degummed comes in accordance with Fig. 1 from the line (1), it is dosed from the Storage container (2) first an aqueous acidic solution, e.g. Citric acid, and one from the storage container (3) aqueous alkaline solution, e.g. Caustic soda, in such Amounts that the oil has a pH of 3 to 6 preferably about 5, through line (4) into one first disperser (5) occurs. In the disperser (5) an oil-water emulsion is created, which can be Line (6) in a retention tank (7) passes.
  • aqueous acidic solution e.g. Citric acid
  • aqueous alkaline solution e.g. Caustic soda
  • the reactor (13) shown has three levels (13a), (13b) and (13c), through which the oil to be treated with a certain dwell time on each floor from top to bottom flows.
  • Each floor can have different Treatment temperatures can be set, it is recommends on the top floor (13a) with the lowest temperature and on the lowest floor (13c) to work with the highest temperature.
  • the Treatment temperatures in the reactor (13) are in the range from 20 to 90 ° C with a total residence time in Reactor (13) of usually 3 to 5 hours.
  • the treated oil leaves the degumming reactor (13) through line (17) and becomes a centrifuge (18) given up.
  • the centrifuge is used as a product degummed oil obtained in the line (19) dissipates.
  • the enzyme-containing aqueous sludge phase in the Line (20) is given from the storage container (21) Separation aid and stir the whole thing in Disperser (22) intensive. Then you give the stirred phase in line (23) one Indwelling container (24), preferably with a Stirring device (25) is provided.
  • the temperatures are in the range from 30 to 85 ° C and preferably at about 60 ° C and man ensures dwell times in the range of 3 to 60 minutes.
  • 2 is correct between the Lines (1) and (17) with the previously together with Fig. 1st described method. 2 is also that coming from the degumming reactor (13) Water-phosphatide sludge-oil mixture through the Line (17) fed to the centrifuge (18), from which one withdraws degummed oil in line (19). Of the enzyme-containing water-sludge phase of the line (30) there one from the reservoir (31) solubilizer to and stir the whole thing in the retention tank (32). Here creates an aqueous, enzyme-containing solution, which one by the line (28) in the reservoir (10) to Reuse returns; an excess of the solution can be removed in line (29). In place the retention tank (32) can also be a Disperser (22), cf. Fig. 1.
  • rapeseed oil which with Water has been degummed in various ways Enzymes degummed.
  • Examples 1, 5 and 6 are Comparative examples in which the invention Procedure is not applied.
  • the degummed rapeseed oil in line (19) has one remaining phosphorus content of 6 ppm, the volume of Water-sludge phase is 370 ml.
  • the phosphorus content is a measure of the level of degumming, good degummed oil has a residual P content of less than 10 ppm.
  • Example 1 For the procedure according to the invention according to FIG. 2 Example 1 is repeated, with the Water-sludge phase of line (30) 3 g / l TWEEN 80 adds and the liquid 10 min in a disperser (Ultra-Turrax) stirs intensely. You get one sludge-free, enzyme-containing solution with no suspended Particles that one in the line (28) in the Returns storage container (10). On adding fresher Enzymes are avoided. The degummed oil of the Line (19) has a residual phosphorus content of 5 ppm per run.
  • a disperser Ultra-Turrax
  • Example 1 For the procedure according to the invention according to FIG. 2 Example 1 is repeated, the one from the Enzyme reactor exiting via line 17 Liquid mixture via line (33) 2.5 g Tween 80 per liter of mixture and the mixture in the Centrifuge 18 separates. You largely get one sludge-free, enzyme-containing solution that can be piped (34) and line (28) to the reservoir (10). Fresh enzymes are not added.
  • the degummed oil in line (19) has one Residual phosphorus content of 5 ppm.
  • Example 1 For the procedure according to the invention according to FIG. 1 Example 1 is repeated, with the Water-sludge phase of the line (20) per liter 2 g TWEEN 80 (manufacturer DuPont) adds and the Liquids in a disperser (22) (Ultra-Turrax) homogenized. After a dwell time of 10 minutes the phosphatide sludge by centrifuging the aqueous enzyme solution separated. The enzyme solution will via the line (28) into the storage container (10) traced back, adding fresher addition No enzyme solution whatsoever. The residual phosphorus content in the degummed oil of line (19) is 5 ppm.
  • Example 4 is modified so that instead of aqueous enzyme solution after centrifugation in the Line (27) occurring, pretreated with TWEEN 80 Phosphatide sludge via the dosing point (9) with the oil the line (8) is mixed. It turns out that this Phosphatide sludge to improve degumming is ineffective because it has no enzyme activity.
  • Example 1 For the procedure according to the invention Example 1 is repeated, taking the water-sludge phase the line (20) 2 liters of alginate protan per liter (Manufacturer: Pronova-Biopolymer, Norway) adds and the liquids in a disperser (22) (Ultra-Turrax) homogenized, cf. Fig. 1. After a The phosphatide sludge becomes a residence time of 10 minutes by centrifuging the aqueous enzyme solution severed. The enzyme solution is in the Storage container (10) returned. Without adding fresh enzymes the residual phosphorus content in degummed oil of line (19) 8 ppm.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)
EP94203211A 1993-11-19 1994-11-04 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen Expired - Lifetime EP0654527B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4339556 1993-11-19
DE4339556A DE4339556C1 (de) 1993-11-19 1993-11-19 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen

Publications (2)

Publication Number Publication Date
EP0654527A1 EP0654527A1 (de) 1995-05-24
EP0654527B1 true EP0654527B1 (de) 1998-01-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP94203211A Expired - Lifetime EP0654527B1 (de) 1993-11-19 1994-11-04 Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen

Country Status (12)

Country Link
US (1) US5558781A (en:Method)
EP (1) EP0654527B1 (en:Method)
JP (1) JPH07188691A (en:Method)
CN (1) CN1046760C (en:Method)
AT (1) ATE162210T1 (en:Method)
BR (1) BR9404496A (en:Method)
CA (1) CA2136050A1 (en:Method)
DE (2) DE4339556C1 (en:Method)
DK (1) DK0654527T3 (en:Method)
ES (1) ES2111841T3 (en:Method)
GR (1) GR3026501T3 (en:Method)
TW (1) TW279900B (en:Method)

Cited By (13)

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US7371423B2 (en) 1997-04-09 2008-05-13 Danisco, A/S Method for preparing flour doughs and products made from such doughs using lipase
US7622290B2 (en) 2004-03-12 2009-11-24 Danisco A/S Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
US7638293B2 (en) 2003-01-17 2009-12-29 Danisco A/S Method
US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

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DK0869167T4 (da) * 1996-12-09 2010-03-08 Novozymes As Reduktion af phosphor-indeholdende bestanddele i spiseolier; som omfatter en stor mængde ikke-hydrerbart phosphor, ved anvendelse af en phospholipase, en phospholipase fra en trådsvamp, der har en phospholipase A og/eller B aktivitet
US6103505A (en) * 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
EP1071734A1 (en) * 1998-04-08 2001-01-31 Novozymes A/S An enzymatic oil-degumming process
EP2302043A3 (en) 1998-11-27 2011-07-20 Novozymes A/S Lipolytic enzyme variants
US6166231A (en) * 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
EP2258853B1 (en) 2000-04-28 2016-06-08 Novozymes A/S Lipolytic enzyme variant
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
JP2005523019A (ja) 2002-04-19 2005-08-04 ダイヴァーサ コーポレイション ホスホリパーゼ、それらをコードする核酸、ならびに、それらの作製方法および使用方法
DE60333629D1 (de) 2002-05-21 2010-09-16 Dsm Ip Assets Bv Neue phospholipasen und deren verwendungen
EP1601332A4 (en) 2003-03-07 2012-05-02 Verenium Corp HYDROLASES, NUCLEIC ACIDS ENCODING THEM, AND METHODS OF MAKING AND USING SAME
PL2468853T3 (pl) 2004-06-16 2015-08-31 Dsm Ip Assets Bv Sposób enzymatycznego odbarwiania feofityny
US20080070291A1 (en) * 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
EP2216403A3 (en) 2006-02-02 2010-11-24 Verenium Corporation Esterases and related nucleic acids and methods
CN100441668C (zh) * 2006-06-09 2008-12-10 中国农业科学院油料作物研究所 超声波强化生物酶油脂脱胶的方法
MX2009003034A (es) 2006-09-21 2009-11-18 Verenium Corp Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas.
DE102006046719A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
WO2008088489A2 (en) * 2006-12-22 2008-07-24 Danisco Us, Inc., Genencor Division Enzyme-assisted de-emulsification of aqueous lipid extracts
US8956853B2 (en) 2007-01-30 2015-02-17 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
US8460905B2 (en) 2007-09-11 2013-06-11 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
ES2523300T3 (es) * 2007-01-30 2014-11-24 Bunge Oils, Inc Desgomado enzimático utilizando una mezcla de fosfolipasas PLA y PLC
WO2009046988A2 (en) * 2007-10-10 2009-04-16 Chr. Hansen A/S Process for producing a water-in-oil emulsion
US8076123B2 (en) * 2007-10-26 2011-12-13 Oilseeds Biorefinery Corporation Emulsification-free degumming of oil
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AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
PL2545150T3 (pl) 2010-03-12 2019-01-31 Dupont Nutrition Biosciences Aps Sposób
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DE102010025764A1 (de) 2010-07-01 2012-01-05 Süd-Chemie AG Phospholipase-Träger-Komplex
US9776105B2 (en) 2010-11-19 2017-10-03 Nalco Company Method for conditioning and processing whole or thin stillage to aid in the separation and recovery of protein and oil fractions
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
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WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
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WO2013160374A1 (en) * 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
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JP2016506440A (ja) 2012-12-19 2016-03-03 バックマン・ラボラトリーズ・インターナショナル・インコーポレーテッドＢｕｃｋｍａｎ Ｌａｂｏｒａｔｏｒｉｅｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｉｎｃｏｒｐｏｒａｔｅｄ バイオオイルの回収方法及びシステム並びにそのための分離補助剤
US9090851B2 (en) 2013-03-13 2015-07-28 Hydrite Chemical Co. Oil extraction method and composition for use in the method
WO2015017045A1 (en) 2013-07-03 2015-02-05 Keclon Sa Modified bacillus cereus phospholipase c protein and method of processing vegetable oil
CN103451012A (zh) * 2013-09-02 2013-12-18 东北农业大学 一种用游离磷脂酶a2对大豆混合油脱胶的方法
CN103451011A (zh) * 2013-09-02 2013-12-18 东北农业大学 一种用固定化磷脂酶a1对大豆混合油脱胶的方法
EP3119862B1 (en) 2014-03-19 2022-09-07 Novozymes A/S Polypeptides having phospholipase c activity and polynucleotides encoding same
US20170058235A1 (en) 2014-05-15 2017-03-02 Novozymes A/S Compositions Comprising Polypeptides Having Phospholipase C Activity and Use Thereof
AR100873A1 (es) * 2014-06-17 2016-11-09 Clariant Produkte Deutschland Gmbh Proceso para el desgomado de composiciones que contienen triglicéridos
US9816050B2 (en) 2014-10-27 2017-11-14 Dean Blankenburg Oil extraction method and composition for use in the method
US10377652B1 (en) 2014-12-05 2019-08-13 Fremont Industries, Inc. Process for enhanced fractionation of recovered waste streams
WO2017015180A1 (en) 2015-07-18 2017-01-26 Ecolab Usa Inc. Chemical additives to improve oil separation in stillage process operations
US10344246B2 (en) * 2017-05-24 2019-07-09 Arisyne Systems, Inc. Oil degumming systems
EP3769322A4 (en) 2018-03-21 2022-01-12 Cargill, Incorporated MINERAL OIL AND SYNTHETIC ESTER DIELECTRIC FLUIDS WITH INCREASED STABILITY
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Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US7371423B2 (en) 1997-04-09 2008-05-13 Danisco, A/S Method for preparing flour doughs and products made from such doughs using lipase
US7781001B2 (en) 1998-07-21 2010-08-24 Danisco A/S Foodstuff
US7718204B2 (en) 1998-07-21 2010-05-18 Danisco A/S Foodstuff
US7972638B2 (en) 1998-07-21 2011-07-05 Danisco A/S Foodstuff
US8163315B2 (en) 1998-07-21 2012-04-24 Danisco A/S Foodstuff
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US8278062B2 (en) 2003-01-14 2012-10-02 Dupont Nutrition Biosciences Aps Method of using lipid acyltransferase
US7955813B2 (en) 2003-01-17 2011-06-07 Danisco, A/S Method of using lipid acyltransferase
US7638293B2 (en) 2003-01-17 2009-12-29 Danisco A/S Method
US7807398B2 (en) 2003-01-17 2010-10-05 Danisco A/S Method of using lipid acyltransferase
US8003095B2 (en) 2003-01-17 2011-08-23 Danisco A/S Method of using lipid acyltransferase
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US8440435B2 (en) 2003-12-24 2013-05-14 Dupont Nutrition Biosciences Aps Method for reducing 1,2-diglyceride content of an edible oil
US8012732B2 (en) 2004-03-12 2011-09-06 Danisco A/S Fungal lypolytic and amylase enzyme composition and methods using the same
US7622290B2 (en) 2004-03-12 2009-11-24 Danisco A/S Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
US7666618B2 (en) 2004-07-16 2010-02-23 Danisco A/S Lipolytic enzyme: uses thereof in the food industry
US8192782B2 (en) 2004-07-16 2012-06-05 Danisco A/S Enzymatic oil-degumming method
US8535900B2 (en) 2004-07-16 2013-09-17 Dupont Nutrition Biosciences Aps Lipolytic enzyme uses thereof in the food industry
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US8652809B2 (en) 2007-08-17 2014-02-18 Dupont Nutrition Biosciences Aps Method for producing ultra-heat treatment milk

Also Published As

Publication number Publication date
ATE162210T1 (de) 1998-01-15
EP0654527A1 (de) 1995-05-24
US5558781A (en) 1996-09-24
GR3026501T3 (en) 1998-07-31
TW279900B (en:Method) 1996-07-01
DE59405028D1 (de) 1998-02-19
DE4339556C1 (de) 1995-02-02
ES2111841T3 (es) 1998-03-16
BR9404496A (pt) 1995-07-11
CN1046760C (zh) 1999-11-24
CA2136050A1 (en) 1995-05-20
DK0654527T3 (da) 1998-03-16
JPH07188691A (ja) 1995-07-25
CN1112156A (zh) 1995-11-22

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