Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D498/22—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains four or more hetero rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
  • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
  • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
  • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
  • C07H19/044—Pyrrole radicals

Definitions

• the invention relates to compounds of the formula
• X is O or S
• Y is O, NH, (H,H), (H,OH) or S; with the proviso that when X is S, Y is not NH. (H,H) or (H,OH);
• S(C 1 -C 7 )alkyl S(C 1 -C 7 )substituted alkyl, SCOR 10 , S(O) n R 11 , NH 2 , N(R 12 ,R 13 ), and N ( acyl, R 14 );
• R 5 and R 6 are independently H, C 1 -C 7 alkyl, C 1 -C 7 substituted alkyl, F, CI, Br, OH, N 3 , -OC 1 -C 7 alkyl, -OC 1 -C 7 substituted alkyl, -OCOR 15 , SH, S(C 1 -C 7 )alkyl,S(C 1 -C 7 ) substituted alkyl,
• R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , and R 20 may be the same or different and each independently is H alkyl or substituted alkyl;
• n 1 or 2;
• R 1 and R 2 may not both be directly attached to the 10-position via a heteroatom and R 3 and R 4 may not both be directly attached to the 11-position via a heteroatom ;
• R 1 ,R 2 , R 3 , or R 4 is selected from the group consisting of OH, N 3 , OC 1 -C 7 alkyl, OC 1 -C 7 substituted alkyl, OCOR 9 , SH, S(C 1 -C 7 )alkyl, S(O) n R 11 , NH 2 , N(R 12 ,R 13 ), and N ( acyl, R 14 ).
• R 2 is H; or wherein R 2 is C 1 -C 3 alkyl substituted with OH, and R 1 is H; and R 3 , R 4 , R 5 , and R 6 are H.
• the most preferred compound of the invention is:
• the invention also relates to a pharmaceutical composition
• a pharmaceutical composition comprising a thereapeutically effective amount of a compound of formula I in combination with a pharmaceutically acceptable carrier.
• the invention also relates to a method of treating tumors which comprises administering to a mammal in need of such treatment an anti-tumor effective amount of a compound of formula I for such purpose.
• the invention also relates to a method for treating psoriasis which comprises administering to a mammal in need of such treatment an anti-psoriatically effective amount of a compound of formula I for such purpose.
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , X, and Y are as described above.
• a heavy dark line denotes a chemical bond coming above the plane of the page.
• a dashed line (...''" ) denotes a chemical bond coming below the plane of the page.
• a wavy line ( ⁇ ) denotes either a chemical bond whose stereochemistry is not known, or a mixture of compounds, one compound having the chemical bond going above the plane of the page and the other compound having the chemical bond going below the plane of the page.
• Enantiomers may be separated, where appropriate, by derivatization or salt formation with an optically pure reagent, followed by separation by one of the aforementioned methods. Alternatively, enantiomers may be separated by chromatography on a chiral support.
• the enantiomer of the isomer just above is a compound of the formula:
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , X, and Y are as described above
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , X, and Y are as described above;
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , X. and Y are as described above;
• Two different compounds of the invention may also be diastereoisomers.
• diastereoisomers For example
• the compounds of formula I can exist in unsolvated as well as solvated forms, including hydrated forms, e.g. the hemihydrate.
• solvated forms including hydrated forms, e.g. the hemihydrate.
• pharmaceutically acceptable solvents such as water, ethanol, and the like are equivalent to the unsolvated forms for the purposes of the invention.
• Those compounds of formula I which contain a basic group such as -CH 2 NH 2 form pharmaceutically acceptable salts.
• the preferred pharmaceutically acceptable salts are nontoxic acid addition salts formed by adding to a suitable compound of the invention about a stoichiometric amount of a mineral acid , such as HCl, HBr, H 2 SO 4 or H 3 PO 4 or of an organic acid such as acetic, propionic, valeric, oleic, palmitic, stearic, lauric, benzoic, lactic, para-toluenesulfonic, methane sulfonic, citric, maleic, fumaric, succinic and the like, respectively.
• a mineral acid such as HCl, HBr, H 2 SO 4 or H 3 PO 4
• organic acid such as acetic, propionic, valeric, oleic, palmitic, stearic, lauric, benzoic, lactic, para-toluenesulf
• alkyl (including the alkyl portions of alkoxy, etc) represents a straight or branched, saturated hydrocarbon chain having from 1 to 7 carbon atoms. The number of carbon atoms may be
• C 1 -C 3 alkyl represents a straight or
• substituted alkyl represents alkyl as defined above wherein one or more hydrogen atoms are replaced by a substituent selected from the group consisting of F, CI, Br, OH, OC 1 -C 7 alkyl,
• OCOR a -COOH, -CHO, -COR b , -CN, SH, S(C 1 -C 7 )alkyl, S(O) n R c , -O(CH 2 CH 2 O) q CH 2 CH 2 OH, -O(CH 2 CH 2 O) p CH 2 CH 2 OCH 3 ,SCOR d ) OS(O) 2 R e , N 3 , NH 2 , N(R f ,R g ), 1-imidazolyl, and N ( acyl, R h ), wherein R a ,R b R c ,R d ,R e ,R f , R g and R h are H or alkyl; n is 1 or 2; and q and p are 1 to 3; provided that the two heteroatoms cannot be directly bonded to the same carbon atom.
• acyl represents a CO-alkyl, a CO-substituted alkyl, a CO-aryl or a CO-aralkyl wherein alkyl, substituted alkyl, aryl and aralkyl are as defined herein .
• aryl represents a mono or bi-cyclic aromatic system.
• preferred aryl groups include those having from 6 to 14 carbon atoms. Representative examples include phenyl, 1-naphthyl, and 2-naphthyl.
• the aryl group may contain additional substituents selected from the group consisting of: halogen atoms (e.g., CI, Br, F, and/or I), alkoxy, alkyl, and amino.
• aralkyl represents an alkyl group as defined above in which an aryl group as defined above replaces one of the alkyl hydrogen atoms.
• Representative examples include -CH 2 phenyl, - CH 2 CH 2 phenyl, 4-hydroxybenzyl, 4-t-butyldimethylsilyloxybenzyl, and the like.
• heteroatom represents any atom other than carbon or hydrogen.
• R is H, Br or CO 2
• R 21 and R 21 is alkyl or, aryl or aralkyl.
• R 22 , R 23 and R 24 are independently H and alkyl.
• R 25 is alkyl, aryl, or aralkyl and R 26 is H, methyl or benzyl.
• a compound of formula II (the compound of formula II wherein R is H is preferred) may be reacted with a compound of formula III in the presence of an acid to obtain a compound of formula IV.
• the acid may be a protic acid selected from the group consisting of HCl, toluenesulfonic acid, camphor sulfonic acid and the like.
• the acid may be a Lewis acid selected from the group consisting of BF3, SnCl 4 , TiCl 4 and the like. The most preferred acid for use in this reaction is camphor sulfonic acid.
• the reaction is carried out in a nonpolar solvent such as toluene, or more preferably CH 2 CI 2 .
• the reaction may be conducted at a temperature in the range of about 0° to about 100°.
• the resulting compound of formula IV may be purified or used directly in the next step of the synthesis of a compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula IV is reacted with an ammoniating agent such as NH3, ammonium carbonate, or more preferably ammonia in a solvent such as dimethylformamide (DMF), or dimethylsulfoxide (DMSO); more preferably DMF at a temperature in the range of about 50° to about 150° to obtain a compound of formula I I .
• an ammoniating agent such as NH3, ammonium carbonate, or more preferably ammonia in a solvent such as dimethylformamide (DMF), or dimethylsulfoxide (DMSO); more preferably DMF at a temperature in the range of about 50° to about 150° to obtain a compound of formula I I .
• the resulting compound of formula I I may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I I may be treated with a reducing agent such as LiAIH 4 or, more preferably NaBH4 to obtain a compound of formula I II .
• a reducing agent such as LiAIH 4 or, more preferably NaBH4 to obtain a compound of formula I II .
• the reaction is carried out in a nonpolar solvent such as tetrahydrofuran(THF) or dioxan, or more preferably THF.
• the reaction may be conducted at a temperature in the range of about 0° to about 50°.
• the resulting compound of formula I II may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column
• a compound of formula I I may be treated with BH 3 to obtain a compound of formula I III
• the reaction is carried out in a nonpolar solvent such as THF or dioxan or, preferably THF.
• the reaction may be conducted at a
• the resulting compound of formula I III may be purified. Purification may be carried out by standard techniques such as column chromatography or
• a compound of formula IV may be treated with a brominating agent such as Br 2 , or more preferably N-bromosuccinimide or phenyltrimethylammonium tribromide to obtain a compound of formula VIII.
• a brominating agent such as Br 2
• phenyltrimethylammonium tribromide to obtain a compound of formula VIII.
• the reaction is carried out in a polar solvent such as dimethylformamide (DMF) or dimethylacetamide (DMAC) or more preferably DMF.
• the reaction may be conducted at a temperature in the range of about 0° to about 30°.
• the resulting compound of formula VIII may be purified or used directly in the next step of the synthesis of a compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula VHI may be treated with copper(I) cyanide to obtain a compound of formula IX
• the reaction is carried out in a polar, aprotic solvent such as DMF or dimethyl acetamide
• the reaction may be conducted at a temperature in the range of about 140° to about 200°.
• the resulting compound of formula IX may be purified or used directly in the next step of the synthesis of a compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula DC may be treated with a base such as sodium hydroxide, potassium carbonate, or more preferably potassium hydroxide to obtain a compound of formula I IV .
• the reaction is carried out in a polar solvent such as DMSO.
• the reaction may be conducted at a temperature in the range of about 50° to about 150°.
• the resulting compound of formula I IV may be purified or used directly in the next step of a synthesis of compound of formula I of the invention.
• Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I IV may be treated with an aqueous acid such as HCl, H 2 SO 4 , or, more preferably trifluoroacetic acid to obtain a compound of formula I I
• the reaction is carried out in a polar solvent such as DMF or methanol, more preferably DMSOThe reaction may be conducted at a temperature in the range of about 0° to about 100°.
• the resulting compound of formula I I may be purified or used directly in the next step of the synthesis of another compound of formula I of the invention. Purification may be carried out by standard techniques such as column chromatography, or crystallization.
• a compound of formula I IV may be treated with acetic acid and a reducing agent such as such as t-BuNH 2 .BH 3, or more preferably NaBH 3 CN to obtain a compound of formula I III
• a reducing agent such as such as t-BuNH 2 .BH 3, or more preferably NaBH 3 CN
• the reaction is carried out in a polar solvent such as DMF, ethanol, or more preferably acetic acid.
• the reaction may be conducted at a temperature in the range of about 25° to about 100°
• the resulting compound of formula I III may be purified. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula IX may be treated with a sulfurating agent such as Na 2 S, H 2 S-pyridine or, more preferably NaSH to obtain a compound of formula I V
• a sulfurating agent such as Na 2 S, H 2 S-pyridine or, more preferably NaSH
• the reaction is carried out in a polar solvent such as DMF or more preferably
• the reaction may be conducted at a temperature in the range of about 20° to about 100°.
• the resulting compound of formula I V may be purified. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I IV may be treated with a sulfurating agent such as Na 2 S, H 2 S- pyridine or, more preferably NaSH to obtain a compound of formula I V
• a sulfurating agent such as Na 2 S, H 2 S- pyridine or, more preferably NaSH
• the reaction is carried out in a polar solvent such as DMF or morepreferably DMSO.
• the reaction may be conducted at a temperature in the range of about 20° to about 100°.
• the resulting compound of formula I V may be purified. Purification may be carried out by standard techniques such as column chromatography .
• a compound of formula I XVII may be treated with a sulfonylating agent such as CH 3 SO 2 CI, C 6 H 5 SO 2 CI, (C 6 H 5 SO 2 ) 2 O or, more preferably R 22 SO 2 CI (wherein R 22 is methyl, phenyl, or p-toluene) to obtain a compound of formula XI.
• a sulfonylating agent such as CH 3 SO 2 CI, C 6 H 5 SO 2 CI, (C 6 H 5 SO 2 ) 2 O or, more preferably R 22 SO 2 CI (wherein R 22 is methyl, phenyl, or p-toluene) to obtain a compound of formula XI.
• the reaction is carried out in a nonhydroxylic solvent such as CH 2 CI 2 , CHCI 3 , or THF containing a base such as pyridine, diisopropylethylamine, or triethyiamine.
• the reaction may
• a compound of formula XI may be treated with an amine such as ammonia, methylamine, or dimethylamine to obtain a
• the reaction is carried out in a polar solvent such as DMF, DMAC, or more preferably DMSO.
• the reaction may be conducted at a temperature in the range of about 25° to about 100°.
• the resulting compound of formula I VIII may be purified or used directly in the next step of the synthesis of another compound of formula I.
• Purification may be carried out by standard techniques such as crystallization.
• a compound of formula XI may be treated with a base such as triethylamine or, more preferably, diazabicycloundecene (DBU) to obtain a compound of formula I VII
• a base such as triethylamine or, more preferably, diazabicycloundecene (DBU)
• DBU diazabicycloundecene
• the reaction is carried out in an aprotic solvent such as THF or more preferably DMSO.
• the reaction may be conducted at a temperature in the range of about 20° to about 120°.
• the resulting compound of formula I VIII may be purified or used directly in the next step of the synthesis of another compound of formula I. Purification may be carried out by a standard technique such as crystallization.
• a compound of formula I IX is reacted with an oxidizing agent such as pyridine-sulfur trioxide in dimethylsulfoxide at a temperature in the range of about 0° to about 50° to obtain an aldehyde of formula I X .
• the resulting compound of formula I X may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a starting materiel of formula I IX may be obtained by following the procedures set forth in Formula Scheme 2.
• a compound of formula I X is reacted with an oxidizing agent such as silver oxide in an aqueous organic solvent such as DMSO-water, or DMF-water at a temperature in the range of about 0° to about 50° to obtain a carboxylic acid of formula I XI .
• the resulting compound of formula I XI may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I XI is esterified with methyl or ethyl iodide in a polar solvent such as DMF or DMSO in the presence of a base such as potassium carbonate or cesium carbonate at a
• the resulting compound of formula I XII may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I XII may also be reacted with ammonia in a polar solvent such as DMSO at a temperature in the range of about 100° to about 150° to obtain an amide of formula I XV . It is preferred that this reaction be run under inert atmosphere such as argon or nitrogen.
• a compound of formula IX II may be reacted ammonia and trimethylaluminum in a nonpolar solvent such as methylene chloride or toluene at a temperature in the range of about 25° to about 100° to obtain an amide of formula I XV .
• a compound of formula I XI or I XV may also be subjected to known degradations of a carboxylic acid or carboxamide function, such as the Curtius and Hoffmann degradations, to provide the corresponding amino compound of formula
• Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I 30 may be converted to an amide of formula I XV by reaction with an activating agent such as ethylchloroformate or pivaloyl chloride in a non-hydroxylic solvent such as CH 2 CI 2 or CHCI 3 at a temperature in the range of about -20° to about 0° in the presence of triethylamine followed by reaction with ammonia at a temperature in the range of about 0° to about 25°.
• the resulting compound of formula I XV may be purified or used directly in the synthesis of another compound of formula I. Purification may be carried out by standard techniques such as column chromatography or crystallization.
• a compound of formula I XV may be reacted with a dehydrating agent such as phosgene-triethlamine or benzene-sulfonylchloride-pyridine in a solvent such as CH 2 CI 2 or CHCI 3 at a temperature in the range of about 0° to about 50° to obtain a cyano compound of formula I XVI .
• a dehydrating agent such as phosgene-triethlamine or benzene-sulfonylchloride-pyridine
• a solvent such as CH 2 CI 2 or CHCI 3
• compound of formula I X may be converted to an oxime of formula I XIII by reaction with a compound such as NH 2 OR 26 wherein R 26 is hydrogen, methyl or benzyl.
• the reaction is run in a polar solvent such as ethanol, DMF or DMSO at a temperature in the range of about 0° to about 50° to obtain a compound of formula I XIII .
• the resulting compound of formula I XIII may be purified by standard techniques such as column chromatography or crystallization
• a compound of formula I X may also be converted to a semicarbazone of formula I XIV by reaction with NH 2 NHCONH 2 in a polar solvent such as methanol, ethanol or DMF, at a temperature in the range of about 0° to about 50° , to obtain a compound of formula I XIV .
• the resulting compound of formula I XIVI may be purified by standard techniques such as column chromatography or crystallization.
• the compounds of formula I of the invention are useful as agents for treating tumors. For example, they are useful as agents in the treatment of tumors of the skin, colon and breast.
• the compounds of the invention reduce colony formation in HT-24 human breast carcinoma and HT-29 human colon carcinoma cells.
• PKC protein Kinase C
• PKC Protein Kinase C
• a reaction mixture (0.25 mL total volume) containing: 20 mM Tris-HCl (pH 7.5); 200 mg/mL histone III-S; 10 mM MgCI 2 ; 5 mM [g-32P]ATP (4 ⁇ 106 cpm/nmol); 200 mM CaCI 2 ; 32 mg/mL of phosphatidylserine; 1.6 mg/mL of 1-oleoyl-2-acetyl-glycerol and various concentrations of compounds of this invention added from a dimethyl-sulfoxide (DMSO) stock solution.
• DMSO dimethyl-sulfoxide
• reaction concentration was 2% w/v. Reactions were started by addition of approximately 7 mg of partially purified rat brain PKC and allowed to proceed at 23°C for 15 minutes. Reactions were terminated by collection of trichloroacetic acid-precipitated phosphorylated histone on GF/C filters using a Brandel Cell Harvester (Model No. M-24-R).
• the anti-psoriatic activity of the compounds of formula I of the invention may be demonstrated in the following experimental protocol.
• Keratinocytes (NHEK): Effects of Compounds of the Invention
• N.S. means not statistically significant.
• the compounds of this invention can be administered in any number of conventional dosage forms, e.g., topical, oral, parenteral, rectal, transdermal, and the like.
• Oral or rectal dosage forms include capsules, tablets, pills, powders, cachets, and suppositories.
• Liquid oral dosage forms include solutions and suspensions.
• Parenteral preparations include sterile solutions and suspensions.
• Topical dosage forms can be creams, ointments, lotions, transdermal devices (e.g., of the conventional patch or matrix type) and the like.
• compositions contemplated by the above dosage forms can be prepared with conventional pharmaceutically acceptable excipients and additives, using conventional techniques.
• pharmaceutically acceptable excipients and additives are intended to include carriers, binders, flavorings, buffers, thickeners, coloring agents, stabilizing agents, emulsifying agents, dispersing agents, suspending agents,
• perfumes perfumes, preservatives lubricants, etc.
• Suitable pharmaceutical acceptable solid carriers are magnesium carbonate, magnesium stearate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose. low melting waxes, cocoa butter and the like.
• Capsules can be made wherein the active compound is inserted into the capsules along with a pharmaceutically acceptable carrier.
• the active compounds of this invention can be mixed with pharmaceutically acceptable excipients or be used in finely divided powder form without excipients for inclusion into the capsules. Similarly, cachets are included.
• Liquid form preparations include solutions,
• Aqueous solutions suitable for oral use can be prepared by adding the active component in water and adding suitable colorants, flavors, stabilizing, sweetening, soiubilizing and thickening agents as desired.
• Aqueous suspensions suitable for oral use can be made by dispersing the active component in finely divided form in water with viscous material, i.e., pharmaceutically acceptable natural or synthetic gums, resins, methylcellulose, sodium
• Formulations for topical application may include the above liquid forms, as well as creams, aerosols, sprays, dusts, powders, lotions and ointments which are prepared by combining an active ingredient according to this invention with conventional pharmaceutical acceptable diluents and carriers commonly used in topical, dry, liquid, cream and aerosol formulations.
• Ointment and creams may, for example, be formulated with an aqueous or oily base with the addition of suitable thickening and/or gelling agents.
• bases may, thus, for example, include water and/or an oil such as liquid paraffin or a vegetable oil such as peanut oil or castor oil.
• Thickening agents which may be used according to the nature of the base include soft paraffin, aluminum stearate, cetostearyl alcohol, propylene glycol, polyethylene glycols, woolfat, hydrogenated lanolin, beeswax, etc.
• Lotions may be formulations with an aqueous or oil base and will, in general, also include one or more of
• stabilizing agents emulsifying agents, dispersing agents, suspending agents, thickening agents, coloring agents, perfumes and the like.
• Powders may be formed with the aid of any suitable pharmaceutically acceptable powder base, e.g., talc, lactose, starch, etc.
• Drops may be formulated with an aqueous base or non- aqueous base also comprising one or more pharmaceutically acceptable dispersing agents, suspending agents, solubilizing agents, etc.
• the topical pharmaceutical compositions may also include one or more preservatives or bacteriostatic agents, e.g., methyl hydroxybenzoate, propyl hydroxybenzoate, chlorocresol, benzalkonium chlorides, etc.
• preservatives or bacteriostatic agents e.g., methyl hydroxybenzoate, propyl hydroxybenzoate, chlorocresol, benzalkonium chlorides, etc.
• topical pharmaceutical compositions may also contain an active compound of this invention in combination with other active ingredients such as antimicrobial agents, particularly antibiotics, anesthetics, analgesics and antipruritic agents.
• active ingredients such as antimicrobial agents, particularly antibiotics, anesthetics, analgesics and antipruritic agents.
• solid form preparations which are intended to be converted, shortly before use, to liquid form
• liquid forms include solutions, suspensions and emulsions.
• solid form preparations are most conveniently provided in unit dose form and as such are used to provide a single liquid dosage unit.
• sufficient solid may be provided so that after conversion to liquid form, multiple individual liquid doses may be obtained by measuring predetermined volumes of the liquid form preparation as with a syringe, teaspoon or other volumetric container. When multiple liquid doses are so prepared, it is preferred to maintain the unused portion of said liquid doses under conditions which retard possible decomposition.
• the solid form preparations intended to be converted to liquid form may contain, in addition to the active material, pharmaceutically acceptable flavorants, colorants, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents and the like.
• the solvent utilized for preparing the liquid form preparation may be water, isotonic water, ethanol, glycerine, propylene glycol and the like as well as mixtures thereof. Naturally, the solvent utilized will be chosen with regard to the route of administration, for example, liquid preparations containing large amounts of ethanol are not suitable for parenteral use.
• the compounds of this invention may also be deliverable transdermally for systemic distribution.
• the transdermal compositions can take the form of creams, lotions and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose.
• composition of the invention comprises a
• the compounds of this invention may be administered by any conventional mode of administration by employing a therapeutically effective amount of a compound of this invention for such mode.
• the dosages may be varied depending upon the requirements of the patient in the judgment of the attending clinician, the severity of the condition being treated and the particular compound being employed. Determination of the proper dosage for a particular situation is within the skill of the art. Treatment can be initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage should be increased by small increments until the optimum effect under the circumstances is reached. For convenience, the total daily dosage may be divided and administered in portions during the day if desired.
• the more polar compound (0.52 gm) was the ⁇ -isomer (7).
• the mole polar isomer was the ⁇ -methoxycompound (13), obtained as an amorphous solid (0.06 g).
• Example 12 compound (10) [0.70 gm, Example 6], was brominated with phenyltrimethylammonium tribromide (PTAB) (1.5 gm) in DMF (25mL) to yield (18) as a white powder (1.1 gm), which was estimated to contain ⁇ 90% of the dibromocompound by PMR spectroscopy, and was used directly in the next step.
• PTAB phenyltrimethylammonium tribromide
• Example 8 was brominated with phenyltrimethylammonium tribromide
• the dinitrile (21) [0.35 g; Example 16] was heated and stirred for 1.25 hours at 120° in a mixture of DMSO (12 mL) and aqueous KOH (1 g KOH in 1.5 mL H 2 O). Water (50 mL) was added, the mixture stirred without heating for 0.5 hours, and the product filtered, washed thoroughly with water and dried at 80° in high vacuum to a yellow powder (25) (0.34 g).
• This reaction was carried out in one reaction vessel.
• the iminocompound (25) [0.05 g; Example 20] was stirred for 20 hours at room temperature in DMSO (3 mL) with powdered sodium hydrogen sulfide (0.25 g) and acetic acid (2 drops). The mixture was diluted with water and extracted with dichloromethane, and the extract was chromatographed on silica gel with dichloromethane. The product (28) eluted as a brown band, orange in solution. Evaporation and crystallization with dichloromethane-hexanes gave a bright red powder (0.003 g)
• Dicyanocompound (21) [0.05 g; Example 16] was heated at 150° for 10 minutes in DMSO (4 ml) with sodium hydrogen sulfide (0.2 g). The mixture was diluted with H 2 O and extracted with dichloromethane, and the extract washed (H 2 O) and chromatographed on deactivated (Grade III) neutral alumina, eluting with dichloromethane. The purple band was collected, evaporated, and the residue triturated in hexanes and vacuum dried to give the dithioimide as a black powder (29) (0.01 g)
• the imide (26) [0.05 g; Example 22] was stirred at room temperature in anhydrous THF (5 mL) and 1 M lithium aluminum hydride-ether (1.0 mL) added. After 0.75 hours, acetic acid (0.2 mL) was added dropwise, and the reaction worked up in water-dichloromethane. The extract was dried and evaporated and the residue trituated with 1 :2 dichloromethane: ether, filtered and dried to an off-white powder (30) (0.025 g).
• the dimethyl compound (4) [0.53 gm; Example 2] was stirred for 24 hours at room temperature in DMF (150 mL) with N-bromo-succinimide
• the dibromocompound (31) [0.64 g; Example 27] was stirred and heated for 20 hours at 180° in DMAC (10 mL), copper (I) cyanide (0.6g) and sodium iodide (0.2 g). The mixture was added to
• the dicyanocompound (32) [0.45g; Example 28] was heated at 125° for 1.5 hours in dimethylsulfoxide (DMSO) (15 mL) with potassium hydroxide (1 g) in water (1.5 mL). Water (50 mL) was added slowly, and the solid was collected by filtration, washed with excess water, then _with methanol and ether, and dried at 80° to a bright yellow powder (33) (0.41 g).
• DMSO dimethylsulfoxide
• the dicyanocompound (23) [0.20 g; Example 18] was heated at 100- 120° for 1 hour in DMSO (10 mL) with KOH (0.8 g) in H 2 O (1.2 mL), cooled, and H 2 O (25 mL) added dropwise. The precipitate was collected, washed well with water, and dried at 80° in vacuum to give the mixture of isomeric iminocompounds (36) as a yellow powder
• the dibenzylester (15) [0.04 gm; Example 10] was heated for 4 hours at 120° in a mixture of DMSO (0.5 mL) and concentrated aqueous ammonia (0.5 mL). After cooling and diluting with water, the product was extracted with ethyl acetate, the extract evaporated, and the residue purified by chromatography on silica gel in hexanes-ethyl acetate to give the imide as a yellow powder (38) (0.02 gm).
• the dicyanocompound (22) [1.00 gm; Example 17] was heated at 150°C for 1.5 hours in DMSO (30 mL) and KOH (2.7 g) in H 2 O (7 mL). After cooling, trifluoroacetic acid (3.3 mL) was added, and the mixture stirred overnight at room temperature. Water (100 mL) was added, and the product filtered, washed well with water and dried at 80° in vacuum to afford 0.74 gm, of (38), identical with material from the preceding example.
• Triester (16) [0.04 gm; Example 11] was heated at 120° for 12 hours in DMF (2 mL) and concentrated aqueous ammonia (2 mL), in a sealed reaction vial. After cooling to room temperature the reaction was diluted with ethyl acetate (25 mL) and washed with water (2 ⁇ 10 mL) and brine (1 ⁇ 15 mL) and then dried (Mg SO 4 ) and concentrated to yield a crude residue. The product (40) was isolated by preparative thin-layer, silicagel chromatography.
• Example 36 was converted to the corresponding mesylate (43) (0.11 gm), a yellow solid.
• camphorsulfonic acid 0.5 g
• CH 2 CI 2 20 mL
• hexanes 20 mL
• silica gel 10 g
• the crude product was chromatographed on flash-grade silica gel, eluting with a gradient from 4:1 to 1:1 hexanes:CH 2 CI 2 , monitoring by thin layer chromatography with 1 :1 CH 2 CI 2 :hexanes.
• Appropriate pure fractions were evaporated to provide the less polar ⁇ - isomer (54) as a white foam (0.37 g) of indefinite melting point.
• n-Bu means n-butyl.
• Example 56 Following the procedure of Example 53, the ⁇ -alcohol (57) (0.33 g; Example 52) was brominated with PTAB (0.65 g) in DMF to give the corresponding dibromocompound (59) (0.50 g) which was used in the next step (Example 56) without further purification.
• Example 54 Following the method of Example 55, the ⁇ -alcohol (59) (0.49 g; Example 54) was converted to the corresponding dicyanocompound (61), a pale brown powder (0.26 g).
• the dicyanocompound (60) (0.11 g; Example 55) was heated at 70-80° for 1.5 hours in DMSO (10 mL) with KOH (0.2 g in 0.5 mL H 2 O), with stirring. After cooling, trifluoroacetic acid (0.5 mL) was added, and stirring continued at room temperature for 2 hours. Water was added, and the product extracted with ethyl acetate. The organic phase was washed with H 2 O (3x), dried and evaporated, and the residue triturated in ether-CH 2 CI 2 , filtered off and dried to give the imide (62) as a bright yellow powder (0.08 g).
• Example 56 Following the procedure of Example 57, the appropriate dicyanocompound (61 ) (0.26 g; Example 56) was converted to the corresponding imide (63), a yellow solid (0.16 g).
• Example 60 Example 60
• Example 65 The procedure of Example 65 was followed, replacing the methylamine hydrochloride with benzylamine hydrochloride. After isolation and chromatography in 2% MeOH-CH 2 Cl 2 , the benzylamide (71) was obtained as a yellow solid (0.034 g).
• the t-butylester (69) (0.025 g; Example 64) was stirred at room temperature for 2 hours in trifluoroacetic acid (2 mL). H 2 O (20 mL) was added. After stirring for 0.5 hours, the precipitate was collected, washed with water and ether, and dried at 50° in high vacuum to give the acid (74) as a deep orange powder (0.011 g).
• N-lodo succimimide (1.3 g) and triphenylphosphine (TPP) (1.68 g) were dissolved in anhydrous THF (50 mL) at 0°C. To this solution was added alcohol (18) (0.6 g, Ex. 13) and stirred for 6 hours at room temperature. The reaction was diluted with 100 mL ethyl acetate and washed (3 ⁇ 50 mL water) to remove succinimide. The organic portion was dried (MgSO 4 ) and concentrated to yield a solid. The solid was washed (2 ⁇ 30 mL hexane) to remove triphenyl phosphine. The resulting crude product was redissolved in 50 mL ethyl acetate and upon concentration the product (80) crystallized and leaving TPP oxide in the solution. The product was collected by filtration and dried to provide 1.2 g of pure product.
• Example 16 The procedure from Example 16 was repeated on dibromide (81) (0.3 g) with CuCN (0.426 g) and Nal (0.213 g). Similar work up provided the dinitrile (82) which was taken to the next step. (Base-acid hydrolysis).

Landscapes

• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Medicinal Chemistry (AREA)
• Pharmacology & Pharmacy (AREA)
• General Chemical & Material Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Dermatology (AREA)
• Biochemistry (AREA)
• Biotechnology (AREA)
• Genetics & Genomics (AREA)
• Molecular Biology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)
• Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)

Applications Claiming Priority (2)

Publications (1)

Family

ID=24745378

Family Applications (2)

Family Applications Before (1)

Country Status (14)

Families Citing this family (17)

Family Cites Families (2)

• 1992
  • 1992-04-09 HU HU9302869A patent/HUT70187A/hu unknown
  • 1992-04-09 JP JP4510240A patent/JPH06503837A/ja active Pending
  • 1992-04-09 CZ CS932136A patent/CZ213693A3/cs unknown
  • 1992-04-09 IL IL101554A patent/IL101554A0/xx unknown
  • 1992-04-09 MX MX9201643A patent/MX9201643A/es unknown
  • 1992-04-09 WO PCT/US1992/002661 patent/WO1992018507A1/en not_active Application Discontinuation
  • 1992-04-09 CA CA002108146A patent/CA2108146A1/en not_active Abandoned
  • 1992-04-09 EP EP92303187A patent/EP0508792A1/de not_active Withdrawn
  • 1992-04-09 TW TW081102729A patent/TW214553B/zh active
  • 1992-04-09 NZ NZ242294A patent/NZ242294A/en unknown
  • 1992-04-09 SK SK1082-93A patent/SK108293A3/sk unknown
  • 1992-04-09 EP EP92917468A patent/EP0580812A1/de active Pending
  • 1992-04-10 IE IE114892A patent/IE921148A1/en unknown
• 1993
  • 1993-10-08 NO NO933611A patent/NO933611D0/no unknown
  • 1993-10-11 FI FI934461A patent/FI934461A0/fi not_active Application Discontinuation

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events