EP0394251A1 - Unite de filtrage a equilibrage de la pression - Google Patents

Unite de filtrage a equilibrage de la pression

Info

Publication number
EP0394251A1
EP0394251A1 EP88905818A EP88905818A EP0394251A1 EP 0394251 A1 EP0394251 A1 EP 0394251A1 EP 88905818 A EP88905818 A EP 88905818A EP 88905818 A EP88905818 A EP 88905818A EP 0394251 A1 EP0394251 A1 EP 0394251A1
Authority
EP
European Patent Office
Prior art keywords
filtration unit
sample receptacle
filtrate
vessel
filtration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP88905818A
Other languages
German (de)
English (en)
Inventor
Andreas Dr.Med. Szabados
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP0394251A1 publication Critical patent/EP0394251A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
    • B01D29/05—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements supported
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D36/00—Filter circuits or combinations of filters with other separating devices
    • B01D36/001—Filters in combination with devices for the removal of gas, air purge systems

Definitions

  • the invention relates to a filtration unit, in particular for medical, microbiological, biochemical, immunological or molecular biological samples, with a sample receiving vessel, a filtrate vessel and with an intermediate
  • Sample receptacle and filtrate vessel arranged, having a filter element filter body, the filtrate vessel being connected to the sample receptacle via the filter element to avoid liquid leakage during the filtration, but is otherwise closed to the outside and possibly also the sample receptacle is closed to the outside is.
  • Such a filtration unit is known ( Figures 3-8 of DE-OS 34 27 114).
  • the filtration in the closed system made possible by this known arrangement offers the great advantage that, on the one hand, contamination of the environment is avoided, both by the filtrate and by the filtration liquid and the filtered particles deposited on the filter element, including possible contamination and odor ⁇ nuisance of the environment by escaping gases; on the other hand, an impairment of the filtration liquid and filtrate due to contamination from the outside and especially through the entry of atmospheric oxygen is avoided from the outset.
  • the fil Tration goes in many with the known arrangement
  • the object of the invention is to provide for a filtration unit of the type mentioned at the beginning, in which at least the filtrate vessel is sealed off from the outside, to accelerate the filtration by facilitating pressure equalization.
  • At least one ventilation duct opens into the interior of the filtrate vessel at a first outlet point above the maximum filtrate level.
  • At least one ventilation channel at a second outlet point preferably, also be provided in the interior of the sample receptacle above the maximum filtration liquid level.
  • an automatic exact pressure equalization with a complete seal towards the outside is obtained by a pressure equalization channel connecting the first outlet with the second outlet bypassing the filtration liquid.
  • the pressure equalization channel be penetrated by a portion of the filter element not covered by the filtration liquid. This arrangement is also particularly favorable in terms of production technology, since separate cutouts in the filter element for the pressure compensation channel are avoided.
  • the pressure compensation channel is only penetrated by the support screen.
  • a particularly advantageous embodiment of the invention in terms of production technology is characterized in that the side wall of the sample receptacle is double-walled and in that the double-wall intermediate space forms a section of the at least one pressure compensation channel.
  • This embodiment is also particularly robust mechanically, which is particularly important for the use of the filtration unit in connection with an ultra-centrifuge.
  • the side wall of the sample receptacle can also be formed with at least one axially extending bore, which forms a section of the pressure compensation channel.
  • the sample receiving vessel is provided with an egg part which has at least one axial bore. This insert can then be provided with axial through holes.
  • the filter body can be designed as a separate part in the form of a cylinder ring, in which the sample receptacle and the filtrate vessel with the respective vessel opening are screwed in from both sides.
  • the filtrate vessel and the sample receptacle are then exclusively connected to one another and accordingly closed off from the outside.
  • the sample container can also be formed in one piece with the filter body, in which case only the filtrate vessel is to be screwed onto the filter body.
  • the sample receptacle is then open at its end remote from the filtrate receptacle to allow the pouring in of the filtrate liquid. After this pouring, the opening can then be closed by a cap or lid.
  • the second outlet point be arranged in the area between the cap or lid and the side wall of the sample receptacle.
  • the inner wall part can be shortened accordingly at its upper end in the case of a double-walled design.
  • the cap is provided with a recess in the area of the cap-side edge of the inner wall part of the double-walled side wall or the cap-side edge of the insert part. This again results in a minimal manufacturing outlay with a maximum distance between the second outlet point and the filtration liquid level.
  • At least one through opening with an outward or inward opening check valve can be arranged in the side wall of the filtrate vessel and / or the sample receiving vessel.
  • the passage opening then forms the ventilation duct or ventilation duct mentioned at the beginning.
  • a through opening is provided in the side wall of the filtrate vessel and / or the sample receptacle, which is closed with a filter part that is essentially only gas-permeable,
  • FIG. 1 shows a side view in section along line I-I in FIG. 2 of a first embodiment of a filtration unit according to the invention
  • FIG. 2 shows a horizontal section of the arrangement in FIG. 1 along line II-II;
  • FIG. 3 shows a horizontal section corresponding to FIG. 2 of a second embodiment
  • FIG. 4 shows a side sectional view of a third embodiment.
  • the filtration unit 10 shown in simplified form in FIGS. 1 and 2 consists of three separately manageable parts, namely a sample receptacle 12 with a vessel opening 18 which can be closed by means of a cap 14 and which can be placed on the vessel opening 20 of a filtrate vessel 16 (in the exemplary embodiment shown screwable).
  • the sample receptacle is formed in one piece with a filter body 22 which, in the form of a hollow cylindrical ring, serves as a support for a filter element.
  • the filter element consists of a support screen 24, which completely covers the inner cross section of the filter body and is arranged in the radial plane, and a circular filter disk 26 resting on the support screen.
  • a support screen 24 which completely covers the inner cross section of the filter body and is arranged in the radial plane, and a circular filter disk 26 resting on the support screen.
  • 3 to 7 of DE-OS 34 27 114 of the filter body can also be designed as a separately manageable part, at the upper axial end of which the sample receptacle can be attached, in particular screwed, with its vessel opening in front.
  • the filtration unit 10 enables filtration in a closed system, since the (only Harbor) Vessel opening 20 of the filtrate vessel is closed by the sample receptacle screwed to the filtrate vessel with connection of the vessel interior 28 of the filtrate vessel 16 via the described filter body 22 to the vessel interior 30 Sample receptacle; the vessel opening 18 of the sample receptacle can in turn be closed using the cap 18.
  • the filtrate liquid in the filtrate vessel increasingly displaces the amount of air or gas originally completely filling the interior 28.
  • the result is a corresponding increase in gas pressure.
  • This corresponds to a lowering of the gas pressure in the interior 30 of the sample receiving vessel 12.
  • the gas pressure equalization due to the passage of gas through the filter disk 26 is difficult and, at least after a fine layer of particles to be filtered off is deposited on the filter disk 26 during the filtration, so difficult that the filtrate ⁇ tion extremely slowed down, if not even interrupted.
  • pressure equalizing channels are provided which bypass the filtration liquid in the sample receptacle 12.
  • the sample receptacle 12 is formed with a double-walled side wall.
  • the outer wall part 32 in the form of a hollow cylinder rotates directly into the likewise hollow cylindrical filter body 22, to which an external thread 34 for screwing on a corresponding internal thread 36 at the upper end of the filtrate vessel 16 can be screwed.
  • the inner wall part 38 which is concentric with the outer wall part, is held at a uniform distance from the inner peripheral surface 43 of the outer wall part 32 by means of webs 40 distributed over its outer circumference. In the embodiment shown, these webs 40 are formed with the inner wall part 38; however, they can also be formed in one piece with the outer wall part 32 or form separate parts.
  • the inner wall part 38 sits on the support sieve 24. However, the support sieve 24 is continued up to the outer wall part 14 and embedded or cast into the transition area between the wall part 14 and the annular filter body 22 there.
  • the outer wall part would accordingly be sealingly connected to the annular filter body with the lower edge of the inner wall part resting on the supporting sieve.
  • the annular space formed between the wall parts 32 and 38 is bounded at the bottom by a flat outer circular ring section 41 of the support screen 24.
  • the double-wall space 42 is connected to the interior 28 of the filtrate vessel 16 via this sieve section 41.
  • a corresponding connection also exists to the interior 30 of the sample receptacle 12, preferably with air entering the interior 30 in the area between the cap 14 and the upper edge of the vessel 12. This can be done by appropriate recesses or bores or shortening of the inner wall part 38 or, as shown, through a corresponding recess 46 in the cap 14, which, when the cap 14 is attached, in particular screwed on, creates a connection between the intermediate space 42 and the interior 30.
  • the filtration unit 10 according to FIGS. 1 and 2 can be handled in the following way:
  • the filtration liquid 48 is filled into the sample receptacle 12 connected to the filtrate vessel 16. Then the cap 14 is put on and the unit 10 is inserted into a corresponding stand with the cap 14 facing upwards, so that the filtration takes place under the influence of the force of gravity. Can accelerate the filtration the unit 10 can also be used in an ultra-centrifuge.
  • the gas pressure equalization that becomes necessary when the liquid passes from the interior 30 via the filter disk 26 into the interior 28 takes place via the intermediate space 42, as indicated by flow arrows A in FIG. 1.
  • the axially extending channels 50 formed between the axially extending webs 40 thus connect the spaces 28 and 30 bypassing the filtration liquid 48 in the interior of the inner wall part 38.
  • the gas Before entering the intermediate space 42, the gas must pass through the annular section 40 of the supporting sieve 24 occur, which prevents this liquid from entering the generally rather narrow space 42. A reduction in the free gas passage cross section through liquid in the intermediate space 42 or even a clogging of the channels 50 is thereby excluded. Conversely, the undesired access of unfiltered filtration liquid is prevented through improper handling of the filtration unit in the opposite direction through the channels 50 to the interior of the filtration vessel 28 with a corresponding impairment of the filtrate.
  • the pressure equalization channels 50 are formed by the space in the double-walled vessel wall of the sample receptacle, in the alternative embodiment shown in FIG. 3 the pressure equalization channels 150 are obtained by providing axially parallel bores 152 in the vessel wall or in an insert 154 to be inserted into the vessel. These bores 152 can be formed, for example, already during the manufacture of the insert 154 or can be drilled subsequently.
  • the advantage of a separate insert part 154 lies primarily in the fact that the lower ends of the bores 152 can in turn be easily closed off by an outer flat circular ring section 140 of the support sieve formed in the outer wall part 132.
  • the filter disc 126 is in turn connected to the outside diameter j _ adjusted the inner diameter of the insert 154 and placed on the support screen.
  • the filtration in the closed system with the cap 114 screwed on again takes place in accordance with the first exemplary embodiment according to FIGS. 1 and 2.
  • the filtration vessel 16 can be closed with a separate lid, as can the lower end of the sample receptacle by screwing a cap onto the external thread 34.
  • openings 280 and 282 are respectively in the side wall 284 of the filtrate in both the filtrate vessel 216 and in the sample receiving vessel 214 ⁇ vessel or the side wall 286 of the sample receptacle 216 is provided.
  • Each passage opening is closed with a disk-shaped filter part 288, which is essentially only gas-permeable, ie impermeable to the filtrate liquid 290 DZW. the filtration liquid 292 above the filter element 294.
  • Both through openings 280 and 282 are placed as far up as possible, that is, in such a way that they cannot be reached by the respective liquid at the maximum level of the filtration liquid 292 or the filtrate liquid 290. This in turn results in the desired pressure equalization during the filtration while maintaining the closed system.
  • the filter body 222 is formed separately in the form of a hollow cylindrical ring having the sieve element 294 in the area of its axial length center, in the upper end of which the sample is placed ⁇ receptacle 214 can be screwed in with its vessel opening in front, and accordingly the filtrate vessel 216 from below.
  • the embodiment can also be used 4, a one-piece design of the filter body with the sample receptacle analogous to FIG. 1 can be provided.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • External Artificial Organs (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)

Abstract

Une unité de filtrage comprend un récipient (12) contenant l'échantillon, un récipient (16) contenant le filtrat et un corps de filtrage (22) pourvu d'un élément de filtrage (24, 26) et agencé entre le récipient contenant l'échantillon et le récipient contenant le filtrat. Afin d'assurer un équilibrage automatique de la pression dans un système fermé, l'intérieur (28) du récipient contenant le filtrat est en communication avec l'intérieur (30) du récipient contenant l'échantillon par au moins un canal (50) d'équilibrage de la pression qui contourne le liquide de filtrage (48).
EP88905818A 1987-07-08 1988-07-08 Unite de filtrage a equilibrage de la pression Pending EP0394251A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19873722563 DE3722563A1 (de) 1987-07-08 1987-07-08 Filtrationseinheit mit druckausgleich
DE3722563 1987-07-08

Publications (1)

Publication Number Publication Date
EP0394251A1 true EP0394251A1 (fr) 1990-10-31

Family

ID=6331146

Family Applications (2)

Application Number Title Priority Date Filing Date
EP88110971A Expired - Lifetime EP0298512B1 (fr) 1987-07-08 1988-07-08 Unité de filtration avec égalisation de pression
EP88905818A Pending EP0394251A1 (fr) 1987-07-08 1988-07-08 Unite de filtrage a equilibrage de la pression

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP88110971A Expired - Lifetime EP0298512B1 (fr) 1987-07-08 1988-07-08 Unité de filtration avec égalisation de pression

Country Status (6)

Country Link
US (1) US5104533A (fr)
EP (2) EP0298512B1 (fr)
AT (1) ATE86737T1 (fr)
AU (1) AU1990388A (fr)
DE (2) DE3722563A1 (fr)
WO (1) WO1989000288A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110095581A (zh) * 2019-03-14 2019-08-06 自然资源部第二海洋研究所 一种用于水下传感器的保护及过滤装置

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US6896848B1 (en) 2000-12-19 2005-05-24 Tekcel, Inc. Microplate cover assembly
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FR2863858B1 (fr) * 2003-12-18 2006-03-17 Eric Peltier Flacon de preparation d'une suspension cytologique a base de fixateur
FR2865408B1 (fr) * 2004-01-22 2006-03-10 P2A Connecteur de securite a aiguille
US7897378B2 (en) * 2004-03-18 2011-03-01 Roche Molecular Systems, Inc. Method and device for purifying nucleic acids
DE102010056292B4 (de) * 2010-12-24 2015-12-17 K+S Aktiengesellschaft Adapter zur Verbindung eines Filters mit einem Nährmedienbehälter zum Zwecke der Funktionsprüfung des Filters
CN102974147B (zh) * 2011-09-02 2015-03-04 田菱精细化工（昆山）有限公司 过滤装置
US8968682B2 (en) * 2013-03-15 2015-03-03 Cytosaver Llc Aspiration-free well plate apparatus and methods
CN113751095B (zh) 2015-12-11 2024-01-09 巴布森诊断公司 用于从全血分离血清或血浆的样品容器和方法
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Also Published As

Publication number Publication date
ATE86737T1 (de) 1993-03-15
EP0298512B1 (fr) 1993-03-10
WO1989000288A1 (fr) 1989-01-12
US5104533A (en) 1992-04-14
EP0298512A1 (fr) 1989-01-11
DE3722563A1 (de) 1989-01-19
DE3879009D1 (de) 1993-04-22
AU1990388A (en) 1989-01-30

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