EP0327629A1 - Verfahren und vorrichtung zur untersuchung von vollblut, anderen körperflüssigkeiten und sonstigen biologischen flüssigkeiten - Google Patents
Verfahren und vorrichtung zur untersuchung von vollblut, anderen körperflüssigkeiten und sonstigen biologischen flüssigkeitenInfo
- Publication number
- EP0327629A1 EP0327629A1 EP19880907057 EP88907057A EP0327629A1 EP 0327629 A1 EP0327629 A1 EP 0327629A1 EP 19880907057 EP19880907057 EP 19880907057 EP 88907057 A EP88907057 A EP 88907057A EP 0327629 A1 EP0327629 A1 EP 0327629A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- carrier
- antigen
- holder
- blood
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
Definitions
- the invention comprises the test reaction for the determination of antibodies or antigens the following steps: fixation of one or more first substances with antigen-specific properties on a support, supply of a second substance mixture, the antibody-specific components to be examined with the first substances during a first incubation, can undergo specific test reactions, to the first fabrics; Application of a first indicator which is uniform for the different reactions and which reacts with components of the second substance mixture attached to the first substances during a second incubation period and thus indicates the specific reaction which may have taken place.
- the present method for the determination of antibodies or antigens can also be carried out by the layperson, whereby the costs of the method are considerably reduced.
- the mixture is poured off after the incubation. This shortens the time required to carry out the method, since pouring can be carried out considerably faster than a washing process.
- a carrier is used on which several first substances with antigen-specific properties are or are fixed; furthermore, it is not a second substance but one second substance mixture, the antibody-specific constituents to be investigated which can undergo specific test reactions with the first substances during a first incubation, is brought together with the first substances, and a uniform first indicator is applied for the different reactions, which is used during a second incubation period reacts with constituents of the second mixture of substances attached to first substances, and thus indicates the specific reaction that may have taken place.
- This development of the invention makes it possible to use a single drop of blood to carry out a large number of antibody and antigen tests in a test batch in a group- or disease-associated manner, by applying a plurality of antigens and / or antibodies to the carrier.
- the selection of the antigens and / or antibodies per test batch depends on the requirements that must be met for a screening of specific groups of people, patients and animals.
- the selection of the antigens or antibodies can also be made according to those for the diagnosis of a clinical picture, a group of symptoms or organ disease.
- this object is achieved by a body with a recess which is adapted in shape to accommodate one or more carriers to which one or more first substances with antigen-specific properties are fixed.
- the recess can be covered in a water-tight manner and is dimensioned such that its contents can be mixed by shaking the body.
- Example 3 Testing Different Carrier Materials for the Blood Drop ELISA.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873725504 DE3725504A1 (de) | 1987-07-31 | 1987-07-31 | Verfahren und vorrichtung zum nachweis von antikoerpern und antigenen |
| DE3725504 | 1987-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP0327629A1 true EP0327629A1 (de) | 1989-08-16 |
Family
ID=6332846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP19880907057 Withdrawn EP0327629A1 (de) | 1987-07-31 | 1988-07-31 | Verfahren und vorrichtung zur untersuchung von vollblut, anderen körperflüssigkeiten und sonstigen biologischen flüssigkeiten |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0327629A1 (enrdf_load_stackoverflow) |
| AU (1) | AU2088188A (enrdf_load_stackoverflow) |
| DE (1) | DE3725504A1 (enrdf_load_stackoverflow) |
| WO (1) | WO1989001156A1 (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3842700A1 (de) * | 1988-12-19 | 1990-06-21 | Boehringer Mannheim Gmbh | Verfahren zur proteinimmobilisierung an einer festphase, so hergestellte protein tragende festphase sowie deren verwendung |
| GB9006154D0 (en) | 1990-03-19 | 1990-05-16 | Insituform Group Ltd | Improvements relating to lining materials for pipelines and passageways and to pipes produced from such materials |
| DE4243375C2 (de) * | 1992-12-21 | 1998-04-23 | Brahms Diagnostica Gmbh | Bestimmungsverfahren zur Bestimmung von Antikörpern in biologischen Flüssigkeiten |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3579306A (en) * | 1969-01-22 | 1971-05-18 | Organon | Diagnostic test device |
| US3826619A (en) * | 1971-12-21 | 1974-07-30 | Abbott Lab | Test apparatus for direct radioimmuno-assay for antigens and their antibodies |
| US4148869A (en) * | 1975-03-06 | 1979-04-10 | Baxter Travenol Laboratories, Inc. | Immunological reagent and method of using same |
| GB1565402A (en) * | 1975-11-03 | 1980-04-23 | Int Diagnostic Tech | Diagnostic reagent holder |
| FI54842C (fi) * | 1977-01-14 | 1979-03-12 | Suovaniemi Finnpipette | Formstycke i anordningar foer immunitetsbestaemningar och enzymreaktioner |
| US4452903A (en) * | 1981-02-17 | 1984-06-05 | Lee Jin P | Assay method and reagent kit means for lipid-containing body fluid |
| EP0061541A1 (en) * | 1981-03-27 | 1982-10-06 | Biospecia Limited | Immunological analysis and a biochemical agent therefor |
| GB2121962B (en) * | 1982-06-08 | 1985-10-02 | Serono Diagnostics Ltd | Manual immunoassays and apparatus therefor |
| JPS59155762A (ja) * | 1982-12-06 | 1984-09-04 | フイ−ルダ−・ジルレスピ−・デイビス・リミテツド | フイ−ルドイムノアツセイ反応システムおよび方法 |
| DE3327642A1 (de) * | 1983-07-30 | 1985-02-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur bestimmung eines partners einer immunreaktion sowie reagens zur durchfuehrung dieses verfahrens |
| US4594327A (en) * | 1983-11-02 | 1986-06-10 | Syntex (U.S.A.) Inc. | Assay method for whole blood samples |
| CA1253070A (en) * | 1984-04-20 | 1989-04-25 | Syntex (U.S.A.), Inc. | Single step heterogenous assay |
| FI860249A7 (fi) * | 1985-02-28 | 1986-08-29 | Becton Dickinson Co | Laite ja menetelmä moninkertaista, samanaikaista koetta varten. |
| US4746631A (en) * | 1985-05-09 | 1988-05-24 | Ultra Diagnostics Corporation | Immunoassay method, device, and test kit |
| EP0204579A3 (en) * | 1985-06-05 | 1987-10-28 | Synbiotics Corporation | Immunoassay incubation device |
| DE3640412A1 (de) * | 1986-11-26 | 1988-06-09 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
-
1987
- 1987-07-31 DE DE19873725504 patent/DE3725504A1/de active Granted
-
1988
- 1988-07-31 EP EP19880907057 patent/EP0327629A1/de not_active Withdrawn
- 1988-07-31 AU AU20881/88A patent/AU2088188A/en not_active Abandoned
- 1988-07-31 WO PCT/EP1988/000693 patent/WO1989001156A1/de not_active Application Discontinuation
Non-Patent Citations (1)
| Title |
|---|
| See references of WO8901156A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3725504A1 (de) | 1989-02-09 |
| AU2088188A (en) | 1989-03-01 |
| DE3725504C2 (enrdf_load_stackoverflow) | 1989-10-05 |
| WO1989001156A1 (en) | 1989-02-09 |
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