EP0327629A1 - Procede et dispositif d'examen de sang complet, d'autres fluides corporels et d'autres fluides biologiques - Google Patents

Procede et dispositif d'examen de sang complet, d'autres fluides corporels et d'autres fluides biologiques

Info

Publication number
EP0327629A1
EP0327629A1 EP19880907057 EP88907057A EP0327629A1 EP 0327629 A1 EP0327629 A1 EP 0327629A1 EP 19880907057 EP19880907057 EP 19880907057 EP 88907057 A EP88907057 A EP 88907057A EP 0327629 A1 EP0327629 A1 EP 0327629A1
Authority
EP
European Patent Office
Prior art keywords
carrier
antigen
holder
blood
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19880907057
Other languages
German (de)
English (en)
Inventor
Olaf Thraenhart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP0327629A1 publication Critical patent/EP0327629A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Definitions

  • the invention comprises the test reaction for the determination of antibodies or antigens the following steps: fixation of one or more first substances with antigen-specific properties on a support, supply of a second substance mixture, the antibody-specific components to be examined with the first substances during a first incubation, can undergo specific test reactions, to the first fabrics; Application of a first indicator which is uniform for the different reactions and which reacts with components of the second substance mixture attached to the first substances during a second incubation period and thus indicates the specific reaction which may have taken place.
  • the present method for the determination of antibodies or antigens can also be carried out by the layperson, whereby the costs of the method are considerably reduced.
  • the mixture is poured off after the incubation. This shortens the time required to carry out the method, since pouring can be carried out considerably faster than a washing process.
  • a carrier is used on which several first substances with antigen-specific properties are or are fixed; furthermore, it is not a second substance but one second substance mixture, the antibody-specific constituents to be investigated which can undergo specific test reactions with the first substances during a first incubation, is brought together with the first substances, and a uniform first indicator is applied for the different reactions, which is used during a second incubation period reacts with constituents of the second mixture of substances attached to first substances, and thus indicates the specific reaction that may have taken place.
  • This development of the invention makes it possible to use a single drop of blood to carry out a large number of antibody and antigen tests in a test batch in a group- or disease-associated manner, by applying a plurality of antigens and / or antibodies to the carrier.
  • the selection of the antigens and / or antibodies per test batch depends on the requirements that must be met for a screening of specific groups of people, patients and animals.
  • the selection of the antigens or antibodies can also be made according to those for the diagnosis of a clinical picture, a group of symptoms or organ disease.
  • this object is achieved by a body with a recess which is adapted in shape to accommodate one or more carriers to which one or more first substances with antigen-specific properties are fixed.
  • the recess can be covered in a water-tight manner and is dimensioned such that its contents can be mixed by shaking the body.
  • Example 3 Testing Different Carrier Materials for the Blood Drop ELISA.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un procédé permet de dépister des protéines à propriétés spécifiques de type anticorps ou antigènes dans des fluides corporels ou d'autres matières organiques. On utilise une protéine fixée sur un support qui entre en réaction spécifique avec la protéine que l'on veut dépister dans la solution, rendant cette dernière insoluble, et une deuxième protéine se trouvant dans la solution, pourvue d'un marqueur, qui entre en réaction avec le composant insoluble et devient elle aussi insoluble. On peut ainsi examiner directement du sang, sans centrifugation préalable, afin d'y dépister simultanément une ou plusieurs protéines.
EP19880907057 1987-07-31 1988-07-31 Procede et dispositif d'examen de sang complet, d'autres fluides corporels et d'autres fluides biologiques Withdrawn EP0327629A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3725504 1987-07-31
DE19873725504 DE3725504A1 (de) 1987-07-31 1987-07-31 Verfahren und vorrichtung zum nachweis von antikoerpern und antigenen

Publications (1)

Publication Number Publication Date
EP0327629A1 true EP0327629A1 (fr) 1989-08-16

Family

ID=6332846

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19880907057 Withdrawn EP0327629A1 (fr) 1987-07-31 1988-07-31 Procede et dispositif d'examen de sang complet, d'autres fluides corporels et d'autres fluides biologiques

Country Status (4)

Country Link
EP (1) EP0327629A1 (fr)
AU (1) AU2088188A (fr)
DE (1) DE3725504A1 (fr)
WO (1) WO1989001156A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3842700A1 (de) * 1988-12-19 1990-06-21 Boehringer Mannheim Gmbh Verfahren zur proteinimmobilisierung an einer festphase, so hergestellte protein tragende festphase sowie deren verwendung
GB9006154D0 (en) 1990-03-19 1990-05-16 Insituform Group Ltd Improvements relating to lining materials for pipelines and passageways and to pipes produced from such materials
DE4243375C2 (de) * 1992-12-21 1998-04-23 Brahms Diagnostica Gmbh Bestimmungsverfahren zur Bestimmung von Antikörpern in biologischen Flüssigkeiten

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3579306A (en) * 1969-01-22 1971-05-18 Organon Diagnostic test device
US3826619A (en) * 1971-12-21 1974-07-30 Abbott Lab Test apparatus for direct radioimmuno-assay for antigens and their antibodies
US4148869A (en) * 1975-03-06 1979-04-10 Baxter Travenol Laboratories, Inc. Immunological reagent and method of using same
GB1565402A (en) * 1975-11-03 1980-04-23 Int Diagnostic Tech Diagnostic reagent holder
FI54842C (fi) * 1977-01-14 1979-03-12 Suovaniemi Finnpipette Formstycke i anordningar foer immunitetsbestaemningar och enzymreaktioner
US4452903A (en) * 1981-02-17 1984-06-05 Lee Jin P Assay method and reagent kit means for lipid-containing body fluid
EP0061541A1 (fr) * 1981-03-27 1982-10-06 Biospecia Limited Analyse immunologique et agent biochimique pour cette analyse
GB2121962B (en) * 1982-06-08 1985-10-02 Serono Diagnostics Ltd Manual immunoassays and apparatus therefor
EP0113075A3 (fr) * 1982-12-06 1985-05-02 Fielder Gillespie Davis Limited Système de réaction d'essai immunologique transportable, procédé pour le diagnostic par essai immunologique et sonde ou support utilisé dans ce procédé
DE3327642A1 (de) * 1983-07-30 1985-02-14 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung eines partners einer immunreaktion sowie reagens zur durchfuehrung dieses verfahrens
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
JPS60249057A (ja) * 1984-04-20 1985-12-09 シンテツクス（ユ−・エス・エイ）インコ−ポレイテツド １段階へテロジニアスアツセイ
FI860249A (fi) * 1985-02-28 1986-08-29 Becton Dickinson Co Apparat och foerfarande foer flerfaldigt, samtidigt prov.
US4746631A (en) * 1985-05-09 1988-05-24 Ultra Diagnostics Corporation Immunoassay method, device, and test kit
JPS6270763A (ja) * 1985-06-05 1987-04-01 シンビオテイツクス　コ−ポレイシヨン 免疫アツセイインキユベ−シヨン装置
DE3640412A1 (de) * 1986-11-26 1988-06-09 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8901156A1 *

Also Published As

Publication number Publication date
AU2088188A (en) 1989-03-01
WO1989001156A1 (fr) 1989-02-09
DE3725504C2 (fr) 1989-10-05
DE3725504A1 (de) 1989-02-09

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