EP0274127A2 - Dérivés de poly(acide aminodicarboxylique hydroxy alkylé) biodégradable, leur procédé de préparation et leur utilisation comme support de médicament à libération contrôlée - Google Patents

Dérivés de poly(acide aminodicarboxylique hydroxy alkylé) biodégradable, leur procédé de préparation et leur utilisation comme support de médicament à libération contrôlée Download PDF

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EP0274127A2
EP0274127A2 EP87119291A EP87119291A EP0274127A2 EP 0274127 A2 EP0274127 A2 EP 0274127A2 EP 87119291 A EP87119291 A EP 87119291A EP 87119291 A EP87119291 A EP 87119291A EP 0274127 A2 EP0274127 A2 EP 0274127A2
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poly
formula
polymer
hydroxyalkyl
aminodicarboxylic acid
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EP87119291A
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German (de)
English (en)
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EP0274127A3 (en
EP0274127B1 (fr
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Hubert Dr. Bader
Diether Dr. Rüppel
Axel Dr. Walch
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Hoechst AG
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Hoechst AG
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G69/00—Macromolecular compounds obtained by reactions forming a carboxylic amide link in the main chain of the macromolecule
    • C08G69/02—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids
    • C08G69/08—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids derived from amino-carboxylic acids
    • C08G69/10—Alpha-amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1629—Organic macromolecular compounds
    • A61K9/1641—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poloxamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2031—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyethylene oxide, poloxamers
    • A61K9/2045—Polyamides; Polyaminoacids, e.g. polylysine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5005—Wall or coating material
    • A61K9/5021—Organic macromolecular compounds
    • A61K9/5031—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poly(lactide-co-glycolide)

Definitions

  • the invention relates to biodegradable poly (hydroxyalkyl) aminodicarboxylic acid derivatives, a process for their preparation and the use thereof for depot preparations with controlled release of active ingredients.
  • the active ingredients are embedded in a matrix consisting of the polyamides according to the invention and released in vivo by bioerosion of the matrix.
  • the products according to the invention are broken down, only the body's own fragments or fragments known in their biocompatibility are formed, which are metabolized in natural metabolic pathways or are excreted by the kidneys on account of their water solubility.
  • Modern drug therapy requires new dosage forms, especially for the application of active ingredients, which combine a controlled rate of release of the active ingredients with high biocompatibility of the depot.
  • a long-term controlled drug delivery is of great topicality due to the increasing importance of chronic diseases and long-term therapy concepts in human and veterinary medicine.
  • Biodegradable polymers are particularly advantageous as matrix materials for such depot systems, since bioerosion controls the release of active ingredient and makes the surgical removal of such a depot unnecessary.
  • the active ingredient is dispersed in a biodegradable polymer which releases the active ingredient upon degradation.
  • Typical biodegradable polymers that have mostly been investigated according to the prior art are homo- and copolyesters, in particular lactic and glycolic acid, as described in US Pat. Nos. 3,773,919 and 3,297,033, respectively.
  • One disadvantage is the low or poorly controllable swellability of the polyester in the physiological environment, which hinders the transport of the active ingredients incorporated in the implant through the polymer matrix to the surface and causes an only low release rate after the initial "burst effect".
  • US Pat. No. 4,356,166 describes biodegradable implant materials which release a bioactive compound in vivo.
  • Progestins are described as bioactive compounds in US Pat. No. 4,356,166, which are first chloroformylated and then covalently bound to the polymer.
  • the polymers used are poly (hydroxyalkyl) -L-glutamine or poly (hydroxyalkyl) -L-aspartamide.
  • the bioactive compounds are bound either via a so-called "spacer group” or directly via the reactive component of the polymer. The rate of release of the bioactive compound is controlled via the molecular weight of the polymer or via the length and the character of the "spacer group".
  • a disadvantage of the substances according to US Pat. No. 4,356,166 is that they are themselves pharmaceuticals with high pharmacological activity.
  • biocompatible polymer and drug form a unit, the properties of which are determined in a complex manner by both components.
  • the release rate of the polymer-fixed bioactive molecule is variable within the parameters mentioned above, however crucially dependent on the nature of the active ingredient.
  • Hydrophobic bioactive substances, such as steroid hormones can only be split off very slowly from the polymer backbone in an aqueous biological environment and are therefore only suitable for extreme long-term depot forms.
  • New polymer / drug conjugates must be synthesized for each drug, which extremely limits the usability of the concept of polymer-fixed pharmaceuticals described in US Pat. No. 4,356,166.
  • Poly (hydroxyalkyl) aminodicarboxylic acid derivatives have now been synthesized which, surprisingly, are outstandingly suitable for use as degradable drug implants with controlled release of active ingredients. It is essential to the invention that the active substances are not chemically bound to the polymer, but rather are only embedded in this polymer matrix. By incorporating suitable biologically inactive acyl groups, the rate of degradation of the polymer in vivo can be controlled in the desired manner, and thus the rate of release of the active ingredient can also be controlled. The advantage of this procedure is that such active substances can now be applied over a longer period of time with a relatively constant dose that either cannot be chemically bound to a polymer at all or are too sensitive to withstand the rather drastic conditions in the chemical process To survive coupling to the polymer.
  • the polymers can be used universally as a pharmacologically inert matrix for all relevant pharmaceuticals, regardless of the molecular size and other physico-chemical parameters.
  • This biodegradable polymer maintains by polycondensation of aminodicarboxylic acids, which are then reacted with amino alcohols to form poly (hydroxyalkyl) aminodicarboxylic acids and then react with carboxylic acids, carboxylic acid halides or halogen formic acid esters in a polymer-analogous acylation to give the desired poly (hydroxyalkyl) aminodicarboxylic acid derivatives.
  • These polymers are metabolized and excreted in vivo to non-toxic, non-allergenic and non-immunogenic compounds.
  • the invention thus relates to: Poly (hydroxyalkyl) aminodicarboxylic acid derivatives of the formula I. in which n is 1 or 2, m is 2 to 6, x and y is 1 to 400 and z is 0 to 40, characterized in that the radicals R and R ⁇ are identical or different from one another and are branched or unbranched, saturated or unsaturated alkyl, Cycloalkyl, alkyloxy or cycloalkyloxy with a total of 1 - 22 carbon atoms in the alkyl part, where the alkyl part can optionally be interrupted by a carbonyloxy group, or biologically inactive steroid alcohols, bonded via their hydroxyl groups, where the monomer units in square brackets are statistically im Polymers are distributed.
  • the invention also relates to the process for the preparation of the abovementioned polyamides and their use, also in mixtures with other, biocompatible polyamides, especially in conjunction with biologically active substances, as a degradable active substance depot preparation with controlled active substance release.
  • Aspartic acid is preferably used, which reacts in a polycondensation reaction to give the corresponding polyanhydroaspartic acid (VII).
  • VII polyanhydroaspartic acid
  • an amino alcohol of the formula IV H2N- (CH2) m -OH IV where m is a number from 2 to 6, 3-aminopropanol, in particular 2-aminoethanol, being preferred, ⁇ , ⁇ -poly- (hydroxyalkyl) -DL-aspartamide of the formula II is obtained
  • PHEA ⁇ , ⁇ -poly- (2-hydroxyethyl) -DL-aspartamide
  • the protective group is split off either by adding an HCl / HBr mixture to the free poly- ⁇ -L-glutamic acid or in the presence of hydroxyalkylamines to the analogous poly- ⁇ - (hydroxyalkyl) -L-glutamines.
  • a general working procedure for the production of poly- ⁇ - (hydroxypropyl) -L-glutamine can be found in US Pat. No. 4,356,166, to which reference is expressly made here.
  • the analogous higher amino alcohol derivatives of poly- ⁇ - (benzyl) -L-glutamate can also be prepared in the same way.
  • poly (hydroxyalkyl) aminodicarboxylic acids of the formula IIa preferably ⁇ , ⁇ -poly- (hydroxylalkyl) -DL-aspartamide
  • X stands for a leaving group, which enables a gentle esterification of the polymer alcohol group. Chlorine, bromine, iodine, imidazolides, anhydrides or hydroxyl, in particular chlorine, are preferred.
  • Suitable radicals R ⁇ are alkyl or cycloalkyl groups with a total of 1 to 22 carbon atoms in the alkyl radicals. Alkyl residues with 5 to 22 carbon atoms, in particular those with 6 to 18 carbon atoms, are preferably used in homopolymers. Particularly preferred in compounds of the formula V are those alkyl radicals R ⁇ with an even number of carbon atoms; in compounds according to formula VI, such alkyl radicals R ⁇ with an odd number of carbon atoms. In the preparation of copolymers, that is to say in the reaction with two or more different compounds of the formula V and / or VI, it is also possible to use compounds with shorter alkyl radicals than those specified in the preferred ranges.
  • a compound with a C-8-alkyl chain together with 50 mol% of a compound with a C-2-alkyl chain can form the poly- (hydroxyalkyl) - aminodicarboxylic acid derivatives are implemented.
  • the alkyl radicals mentioned can be branched, but preferably unbranched. Unsaturated, but preferably saturated, alkyl radicals can also be used. Furthermore, the alkyl radical can also be interrupted by a carbonyloxy group. Steroids, in particular cholesteryl, are also suitable as R ⁇ radicals.
  • reaction with the compounds of formula type V or VI can take place either with a single such compound or with any combination of these compounds or with compounds which have different, e.g. have different radicals R ⁇ in the way they branch, in particular in their chain length.
  • the latter polymer-analogous acylation is carried out according to known methods of organic chemistry, such as e.g. in Houben-Weyl, Methods of Organic Chemistry, Vol. VIII (3, pp. 543 ff., G. Thieme Verlag, Stuttgart, 1952). It runs selectively on the hydroxyl function to form esters or carbonates without attacking further functions on the starting polymer.
  • the unicorn variant of Schotten-Baumann acylation in the presence of pyridine is particularly suitable. Under gentle conditions, very high degrees of derivatization (greater than 70%) are achieved.
  • the polymer can be composed of up to three different monomer units (in formula I in square brackets) which are then statistically distributed in this polymer.
  • the chlorine compounds are preferred among the carboxylic acid halides and halogen formic acid esters.
  • the chloroformic acid esters which are preferably used as starting substances are obtained by reacting phosgene with the corresponding biologically inactive, physiologically acceptable, aliphatic or cycloaliphatic, in particular unbranched alcohols. Alcohols which have an even number of carbon atoms are particularly preferably used.
  • the chloroformylated steroids are also obtained in this way. In principle, all biologically inactive steroids with reactive hydroxyl groups are accessible. Examples include: cholesterol, cholestanol, coprostanol, ergosterol, sitosterol or stigmasterol.
  • the acid chlorides which can likewise preferably be used as starting compounds are obtained, for example, from the corresponding carboxylic acids by reaction with phosphorus trichloride, phosphorus pentachloride, oxalyl chloride or thionyl chloride (Houben-Weyl, Meth. D. Org. Chem., 4th ed., Vol. VIII, p. 463 ff., Thieme Verlag Stuttgart 1952; or Houben-Weyl, Meth. d. Org. Chem. 4th ed., Erg. Vol. E5, p. 587 ff., Thieme Verlag Stuttgart, 1985).
  • the hydrophobicity of the poly (hydroxyalkyl) aminodicarboxylic acid derivatives - and thus the length of time that an implant made therefrom remains in the organism - can be adjusted within wide limits both by the number of C atoms in the acylation agent and by the degree of substitution.
  • the corresponding derivatives with at least 6 carbon atoms in the alkyl part are already water-insoluble.
  • the degradation time depends on a number of other parameters in addition to the chain length; for example the particle size and distribution, the manufacturing method e.g. for microspheres, the porosity of the microspheres, the temperature or the degradation medium.
  • the degree of substitution can be changed via the stoichiometry of the substances used in the acylation reaction, but should preferably be kept within the maximum yield (greater than 70%), ie the largest possible percentage of the substitutable OH groups on the polymer backbone should be esterified. If a lower degree of substitution is desired, the concentration of the acylating agent in relation to the polymer is correspondingly reduced.
  • the ester is split again and the corresponding biologically inactive carboxylic acids or alcohols and poly (hydroxyalkyl) aminodicarboxylic acid are formed.
  • This degradation under physiological conditions should ideally only produce fragments that are endogenous to the body or known to be highly biocompatible, which are metabolized in natural metabolic pathways or excreted by the kidneys due to their water solubility.
  • the biocompatible carboxylic acids or alcohols include those with 6 - 22 carbon atoms in the alkyl part, in particular those with an even number of carbon atoms or the biologically inactive steroids such as e.g. Cholesterol.
  • the polymer preferably the neutral PHEA, is regressed, which is extremely soluble due to its strong interaction with water.
  • the new polymers differ from derivatives of poly- ⁇ -amino acids, which are also used in experiments, e.g. Poly- ⁇ -L-glutamic acid esters, from which biodegradation produces polyelectrolytes, which can give rise to toxicological and immunological complications, especially when repeated implantation.
  • the partial stiffness caused by the amide bonds which is caused by hydrogen bonds (N ... H ... O), results in a number of processing advantages of this class of polymers according to the invention.
  • acylation with suitable reactive Carboxylic acids or alcohols on the hydroxyalkyl function of the polymer form hydrophobic polyamides (when the alkyl part has at least 6 carbon atoms), which dissolve in a variety of organic solvents and can be processed into films from the solutions.
  • the polyamides according to the invention are thermoplastic and are therefore suitable for producing active substance depot forms by various methods, such as, for example, by compression, extrusion, precipitation, spraying, etc.
  • the polyamides according to the invention can be used to produce implantable particles, in particular microcapsules and microspheres, by known methods, and by compacting macroscopic shaped articles of any geometry, in particular tablets and rods.
  • the polyamide can, for example, be dissolved with the active ingredient in a suitable polar aprotic solvent, for example dimethyl sulfoxide or dimethylacetamide.
  • a suitable polar aprotic solvent for example dimethyl sulfoxide or dimethylacetamide.
  • an emulsifier With the addition of an emulsifier, the solution is emulsified into an oil phase (e.g. paraffin) at a temperature at which the polymer solution is liquefied. After a few minutes, the individual solvent / polymer droplets are solidified by cooling the emulsion.
  • the polymer balls are cured by washing with a suitable solvent in which the solvent used to dissolve the polyamide and the oil phase dissolve, but not the polymer droplets. Their volume decreases, the shape does not change.
  • the excellent solubility of the polyamides according to the invention in organic solvents also enables the formation of microspheres by dropletization from a solvent with a high melting point into a condensed cold gas, for example liquid nitrogen, whereby absolutely round particles are formed by the Leidenfrost phenomenon.
  • a condensed cold gas for example liquid nitrogen
  • the high-melting and water-miscible solvents are dissolved out by transferring the microspheres into water and the polymer is precipitated, the spherical shape of the polyamide microspheres being retained.
  • this dropletization process can be further simplified by the solvent, e.g. tert. Butanol, directly by freeze-drying, the microspheres obtained by dropping in liquid nitrogen can be removed gently and without loss of active ingredient.
  • the solvent e.g. tert. Butanol
  • the solubility of the polyamides according to the invention in many, also physiologically compatible, solvents, for example alcohols, has a particularly advantageous effect on processing into microspheres by means of spray drying.
  • solvents for example alcohols
  • the use of toxicologically questionable halogenated hydrocarbons, as are required for the spray drying of biodegradable polyesters, can be dispensed with in the polyamides according to the invention.
  • their solubility even in alcohol / water mixtures allows the production of monolithic microspheres containing the active substance, since polymer and active substance can be sprayed from a molecularly disperse form.
  • polyamides according to the invention can also be used as mixtures and in blends with other biodegradable and / or biocompatible polymers (for example ®Pluronic F68, PHEA, dextrans, polyethylene glycols, hydroxyethyl starch and other degradable or separable polysaccharides) or physiologically acceptable auxiliaries (for example polymer plasticizers).
  • biodegradable and / or biocompatible polymers for example ®Pluronic F68, PHEA, dextrans, polyethylene glycols, hydroxyethyl starch and other degradable or separable polysaccharides
  • physiologically acceptable auxiliaries for example polymer plasticizers
  • the product is used for further cleaning Dissolved methanol, precipitated by dropping in 10% aqueous acetic acid solution, suction filtered, washed intensively with water and dried in vacuo. 5 g of a thread-pulling, almost white polymer are obtained which dissolves in DMF, dichloromethane / methanol and methanol and melts above 180 ° C.
  • Example 2 As indicated in Example 1, 3.16 g (20 mmol) of PHEA are reacted with 4.77 g (25 mmol) of 2-cyclohexylethyl chloroformate. About 5 g of a pale yellow colored thermoplastic polymer are obtained, on which no free primary alcohol groups can be detected by NMR spectroscopy (degree of substitution greater than 90%).
  • the acid chloride used for the reaction (4-chloro-4-oxobutyric acid n-butyl ester) is prepared in the following way: Excess oxalyl chloride and a drop of DMF are added to succinic acid monobutyl ester, the reaction starting with evolution of gas. The mixture is left to stand overnight with the exclusion of moisture and then the excess oxyalyl chloride is removed at 40 ° C. on a rotary evaporator. The product has IR bands at 1800 cm ⁇ 1 (acid chloride) and 1740 cm ⁇ 1 (ester) of the same intensity and is used because of its fragility without further purification.
  • Example 7 40 mg of C14-PHEA from Example 7 are dissolved in 1 ml of methylene chloride / methanol (50/1 by volume). The solution is mixed with 10 mg buserelin, which is dispersed with ultrasound. The dispersion is introduced with stirring (800 rpm) into a beaker with 60 ml of 0.1% by weight aqueous polyvinyl alcohol solution (®Mowiol 28-99), which is mixed with 0.3 ml of methylene chloride / methanol (50/1 ) is saturated.
  • aqueous polyvinyl alcohol solution ®Mowiol 28-99
  • the contents are placed in a beaker with 200 ml of water and stirred for 30 minutes (200 rpm).
  • the supernatant water is decanted off and the microspheres are lyophilized (diameter after lyophilization: 20-90 ⁇ m).
  • the solution is introduced into a beaker with 60 ml of 0.1% by weight aqueous solution of carboxymethyl cellulose (®Serva, 300 cps) at 4 ° C., which is saturated with 0.3 ml of methylene chloride, with stirring (800 rpm) . After 5 minutes, the content is worked up according to Example 10.
  • carboxymethyl cellulose ®Serva, 300 cps
  • microspheres are transferred to 200 ml of water and extracted from residual solvent for 2 hours. Excess water is decanted off and the microspheres are lyophilized (diameter after lyophilization 1-2 mm, softening range 190-210 ° C).
  • Example 5 400 mg of polyamide according to Example 5 is dissolved in 8 ml of 90% ethanol and combined with a solution of 100 mg of PHEA and 100 mg of buserelin in 1 ml of water. The mixture is sprayed into microspheres in a spray dryer.
  • the water absorption (in% by weight) of differently substituted polyhydroxyethylaspartamides is determined after 74 h of storage at 92% relative atmospheric humidity, and the duration of the hydrolysis of the alkyl ester / alkyl carbonate side groups (in hours) until complete solubilization of 100 mg of polymer powder in each 100 ml aqueous NaOH (pH 13):
  • the phosphate buffer is stabilized with 0.0078 mol NaN3 against microbial attack and corrected in its pH after every 7 days.
  • the weight loss of the polymer samples is measured over a period of 150 days: the buffer solution with incubated polymer is filtered through a tared glass frit, the residue is dried in a vacuum over phosphorus pentaoxide for 24 hours and the weight loss is determined.
  • An intimate mixture of powdered polymers, additives and active ingredients is used in a suitable device, e.g. an extruder for thermoplastics, heated above the softening point, creating a deformable mass.
  • a suitable device e.g. an extruder for thermoplastics
  • additives and active ingredient (s) are homogeneously dispersed in the softened polymer and the polymer / active ingredient suspension obtained is pressed through a nozzle of a suitable diameter (> 0.5 mm).
  • a suitable diameter > 0.5 mm
  • Example 10 microspheres with a composition of 88% by weight LEMF 6% by weight of C2-C8-PHEA from Example 2 6% by weight buserelin produced.
  • LEMF polylysinethyl / methyl ester fumaramide is the subject of German patent application No. P 36 16 320.1 (example 8) and is produced, for example, by polycondensation of fumaric acid chloride, Lysine methyl ester dihydrochloride and Lysine ethyl dihydrochloride.
  • the drug release buserelin release is measured in a buffer solution (2.91 g Na2HPO4, 0.540 g NaH2PO4, 0.4 g NaN3, 6.328 g NaCl and 2.52 g NaHCO3 on 1 l water) by UV spectroscopy. 1 shows the total amount of buserelin released (in percent) as a function of time.

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EP87119291A 1987-01-03 1987-12-29 Dérivés de poly(acide aminodicarboxylique hydroxy alkylé) biodégradable, leur procédé de préparation et leur utilisation comme support de médicament à libération contrôlée Expired - Lifetime EP0274127B1 (fr)

Priority Applications (1)

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AT87119291T ATE97930T1 (de) 1987-01-03 1987-12-29 Biologisch abbaubare poly-(hydroxyalkyl)aminodicarbons|ure-derivate, verfahren zu ihrer herstellung und verwendung derselben fuer depotzubereitungen mit kontrollierter wirkstoffabgabe.

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DE19873700128 DE3700128A1 (de) 1987-01-03 1987-01-03 Biologisch abbaubare poly- (hydroxyalkyl)- aminodicarbonsaeure-derivate, verfahren zu ihrer herstellung und verwendung derselben fuer depotzubereitungen mit kontrollierter wirkstoffabgabe
DE3700128 1987-01-03

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EP0274127A2 true EP0274127A2 (fr) 1988-07-13
EP0274127A3 EP0274127A3 (en) 1990-06-27
EP0274127B1 EP0274127B1 (fr) 1993-12-01

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US (2) US4906473A (fr)
EP (1) EP0274127B1 (fr)
JP (1) JPH0825909B2 (fr)
AT (1) ATE97930T1 (fr)
DE (2) DE3700128A1 (fr)

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EP0406623A2 (fr) * 1989-07-04 1991-01-09 Röhm GmbH Dérivés de l'acide polyaspartique comme agents d'enrobage pour des formes pharmaceutiques et des aliments
EP0439846A1 (fr) * 1990-01-31 1991-08-07 Hoechst Aktiengesellschaft Polymères biodégradables, procédé de leur préparation et leur utilisation comme support de médicament à libération contrôlée
EP0458079A2 (fr) * 1990-04-26 1991-11-27 Hoechst Aktiengesellschaft Agent de contraste ultrasonique, sa préparation et application comme diagnostic ou thérapeutique
US5190982A (en) * 1990-04-26 1993-03-02 Hoechst Aktiengesellschaft Ultrasonic contrast agents, processes for their preparation and the use thereof as diagnostic and therapeutic agents
US5205287A (en) * 1990-04-26 1993-04-27 Hoechst Aktiengesellschaft Ultrasonic contrast agents, processes for their preparation and the use thereof as diagnostic and therapeutic agents
EP0548794A2 (fr) * 1991-12-20 1993-06-30 Hoechst Aktiengesellschaft Dérivés de polyaspartamide adsorbant les acides biliaires, dérivés de polyaspartamide chargés d'acides biliaires et leur procédé de préparation et leur utilisation comme médicament
EP0823402A2 (fr) * 1996-07-19 1998-02-11 Nalco Chemical Company Utilisation de polymères biodégradables pour empêcher la corrosion et la formation de tartre
EP0831066A1 (fr) * 1996-09-20 1998-03-25 Nalco Chemical Company Polymères biodégradables à base d'acide aspartique pour empêcher la formation de tartre dans les chaudières
US5902357A (en) * 1996-08-30 1999-05-11 Bayer Aktiengesellschaft Composition for dyeing or printing textile materials

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US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5693338A (en) 1994-09-29 1997-12-02 Emisphere Technologies, Inc. Diketopiperazine-based delivery systems
US5629020A (en) * 1994-04-22 1997-05-13 Emisphere Technologies, Inc. Modified amino acids for drug delivery
US6221367B1 (en) 1992-06-15 2001-04-24 Emisphere Technologies, Inc. Active agent transport systems
US6099856A (en) 1992-06-15 2000-08-08 Emisphere Technologies, Inc. Active agent transport systems
US5714167A (en) 1992-06-15 1998-02-03 Emisphere Technologies, Inc. Active agent transport systems
US5578323A (en) 1992-06-15 1996-11-26 Emisphere Technologies, Inc. Proteinoid carriers and methods for preparation and use thereof
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EP0406623A2 (fr) * 1989-07-04 1991-01-09 Röhm GmbH Dérivés de l'acide polyaspartique comme agents d'enrobage pour des formes pharmaceutiques et des aliments
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DE3700128A1 (de) 1988-07-14
DE3788344D1 (de) 1994-01-13
EP0274127A3 (en) 1990-06-27
EP0274127B1 (fr) 1993-12-01
JPS63174937A (ja) 1988-07-19
JPH0825909B2 (ja) 1996-03-13
ATE97930T1 (de) 1993-12-15
US5041291A (en) 1991-08-20
US4906473A (en) 1990-03-06

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