Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

• the present invention relates to an enzymatic dishwashing or rinsing composition comprising lipolytic enzymes.
• Enzymatic dishwashing compositions have been proposed in the art. As enzymes, mainly amylases and/or proteases have been proposed for inclusion in dishwashing compositions. Lipases have also been suggested, but have received far less attention than the amylases and/or proteases. Dishwashing compositions, in particular machine dishwashing compositions which are used in the main wash step of a machine dishwashing operation, have in general a satisfactory cleaning performance. However, frequently the articles cleaned with such products still do not have a satisfactory visual appearance after rinsing and drying, showing film or spots.
• main wash liquor is usually carried over from the main wash step to the rinse step, causing some deposition of soil resulting in insoluble calcium salts on the articles to be rinsed, which results in visible film or spots on the articles when they are dry. In particular with glass articles, this causes an unsightly visual appearance.
• the lipases used according to the present invention, may be of any suitable origin such as yeasts, fungi and bacteria. Preferably, however, they are of bacterial origin, and particularly preferably they belong to the class of bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL B-3673.
• This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division at Peoria, Illinois, under the number NRRL B-3673.
• This lipase will hereinafter be referred to as "Toyo Jozo" lipase.
• the preferred lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133 , pages 76-79 (1950)).
• the preparation of the antiserum is carried out as follows: Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme: day 0 : antigen in complete Freund's adjuvant day 4 : antigen in complete Freund's adjuvant day 32 : antigen in incomplete Freund's adjuvant day 60 : booster of antigen in incomplete Freund's adjuvant.
• the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
• the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
• All lipases showing a positive immunological cross reaction with the Toyo Jozo lipase antibody as hereabove described are preferred lipases according to the present invention.
• Typical examples thereof are the lipases ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp.
• Chromobacter viscosum e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli .
• the lipases of the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
• lipases can be used in their nonpurified form, or in a purified form, e.g. purified with the aid of well-­known adsorption methods, such as a phenylsepharose-­adsorption techniques.
• the composition of the invention may furthermore comprise the usual ingredients of dishwashing or rinse compositions.
• it may contain one or more alkali salts commonly used in dishwashing compositions.
• it may contain organic and/or inorganic builders such as the alkali metal ortho-, pyro and tripolyphosphates and hexametaphosphates, silicates, carbonates, zeolites, borates, citrates, carboxymethyloxy-­succinates, nitrilotriacetates and ethylenediamine-­tetraacetates, polymeric polyelectrolytes such as polyacrylates, polymaleates, and other known organic and inorganic builder compounds.
• the amount of builders in the composition varies from 10-90% by weight, generally from 30-70% by weight.
• the composition may also contain a detergent-active compound. If a detergent-active compound is included, it usually is in an amount of from 0.5-10%, usually 1-­5%. Any well-known type of detergent active compound may be used, such as soaps, synthetic anionic, non-­ionic, amphoteric detergent surfactant and mixtures thereof. Preferably, a nonionic detergent surfactant is used, especially a low-foaming one. Suitable examples of such nonionic detergent surfactants can easily be found in M.Schick "Nonionic Surfactants" (1967).
• composition may furthermore contain other useful additives such as bleaching agents, bleaching agent activators, hydrotropes, fillers, perfumes, colouring agents, germicides, soil-suspending agents, aminopoly­phosphonic acids and alkali metal or alkaline earth metal salts thereof, clays such as hectorites, anti-­corrosion agents such as fatty acids, benztriazole and so on.
• Other enzymes such as proteases, amylases, oxidases may also be included.
• the peroxygen type bleaching agents preferably with a bleach precursor such as TAED are suitable for inclusion in the machine dishwashing compositions.
• a typical example of a conventional machine dishwashing composition usually contains an alkali metal tripoly­phosphate in an amount of from 20-60%, an alkali metal silicate in an amount of from 40-80%, or an alkali metal disilicate in an amount of 5-30% by weight a peroxy type bleaching agent in an amount of from 1-15%, a low-foaming detergent surfactant in an amount of from 0.5-5%, and minor ingredients such as perfumes, colouring agents, hydrotropes, fillers, etc.
• a rinse composition When formulated as a rinse composition, it may contain from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc., the balance being water.
• a nonionic surfactant from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc.
• the balance being water.
• the products of the invention can be formulated in any desirable form, such as powders, granulates, cakes, bars, pastes, liquids etc. The invention will further be illustrated by way of example.
• the detergent composition had the following formulation:
• the Toyo Jozo lipase was used as lipase.
• the test was carried out in two runs, one without and one with lipase.
• the glasses were appraised by an expert panel as to their visual appearance, and were given a score according to the following scale:
• Example 1 The test of Example 1 was repeated, but under the following different conditions: wash temperature : 65°C soil : standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
• wash temperature 65°C soil : standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
• run 1 no lipase added in the final rinse
• run 2 2.5 g Toyo Jozo lipase was added to the final rinse (15 LU/ml).
• the main wash composition had the following formulation:
• Example 2 Replacing the Toyo Jozo lipase by the Amano-P lipase or Lipase MY (ex Candida cylindracea ) or Amano AP-6 lipase (ex Aspergillus niger ) in Example 2 gave similar results.

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• Wood Science & Technology (AREA)
• Organic Chemistry (AREA)
• Detergent Compositions (AREA)

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• 1986
  • 1986-12-10 GB GB868629538A patent/GB8629538D0/en active Pending
• 1987
  • 1987-12-02 DE DE8787202387T patent/DE3772726D1/de not_active Expired - Fee Related
  • 1987-12-02 EP EP87202387A patent/EP0271155B2/de not_active Expired - Lifetime
  • 1987-12-02 ES ES87202387T patent/ES2025635T5/es not_active Expired - Lifetime
  • 1987-12-08 AU AU82223/87A patent/AU609755B2/en not_active Ceased
  • 1987-12-08 JP JP62310794A patent/JPS63161086A/ja active Pending
  • 1987-12-09 BR BR8706685A patent/BR8706685A/pt not_active IP Right Cessation
  • 1987-12-10 ZA ZA879297A patent/ZA879297B/xx unknown

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