EP0271156B1 - Enzyme enthaltendes Geschirrwaschmittel - Google Patents

Enzyme enthaltendes Geschirrwaschmittel Download PDF

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Publication number
EP0271156B1
EP0271156B1 EP87202396A EP87202396A EP0271156B1 EP 0271156 B1 EP0271156 B1 EP 0271156B1 EP 87202396 A EP87202396 A EP 87202396A EP 87202396 A EP87202396 A EP 87202396A EP 0271156 B1 EP0271156 B1 EP 0271156B1
Authority
EP
European Patent Office
Prior art keywords
lipase
pseudomonas
lipases
composition
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP87202396A
Other languages
English (en)
French (fr)
Other versions
EP0271156A3 (en
EP0271156A2 (de
Inventor
Willem Robert Van Dijk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever PLC
Unilever NV
Original Assignee
Unilever PLC
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever PLC, Unilever NV filed Critical Unilever PLC
Publication of EP0271156A2 publication Critical patent/EP0271156A2/de
Publication of EP0271156A3 publication Critical patent/EP0271156A3/en
Application granted granted Critical
Publication of EP0271156B1 publication Critical patent/EP0271156B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the present invention relates to an enzymatic dishwashing composition comprising proteases and a special class of lipases.
  • Lipases are also known in connection with detergents.
  • EP-A 0 130 064 (Novo) describes various detergent and cleaning compositions based on the lipase from Fusarium oxysporum, including compositions containing protease.
  • lipases as a general class of enzymes have also been suggested, no specific proposals relating to the use of specific lipases in dishwashing compositions have been made as far as we know.
  • proteases which can be used in the present invention can be any type of protease known for inclusion in detergent compositions.
  • Most commercially available proteases are of the subtilisin type, and suitable examples of such proteases are Alcalase, Esperase and Savinase, sold by NOVO Industri; Maxatase and Maxacal, sold by Gist Brocades; Optimase sold by Kali Chemie;
  • Kazusase sold by Showa Denka. Preferred are the so-called high alkaline proteases such as Savinase.
  • the class of lipases to be used according to the present invention embraces those lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL-B 3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo KK, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division at Peoria, Illinois, under the number NRRL-B 3673.
  • This lipase will hereinafter be referred to as "Toyo Jozo" lipase.
  • the lipases of the present invention should show a positive immunological cross reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133, pages 76-79 (1950)).
  • the preparation of the antiserum is carried out as follows:
  • Equal volume of 0.1 mg/ml antigen and of Freund's adjuvant are mixed until an emulsion is obtained.
  • Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme:
  • the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 2 5 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • Typical examples thereof are the lipase ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P) , the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolvticum FERM P 1338, the lipase ex Pseudomonas sp., available under the trade name Amano-CES, the lipase ex Pseudomonas cepacia, lipases ex Chromobacter viscosum, e.g. Chromobacter viscosum var.
  • lipolyticum NRRL B-3673 commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas aladioli.
  • the lipases of the present invention are included in the dishwashing composition in such an amount that the final dishwashing composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
  • lipases can be used in their non-purified form, or in a purified form, e.g. purified with the aid of well-known adsorption methods, such as phe- nylsepharose adsorption techniques.
  • the dishwashing compositions of the present invention may furthermore comprise the usual ingredients of dishwashing compositions.
  • they may comprise a small amount, in the order of 0.5 - 5 % by weight of a detergent surfactant, e.g. anionic or nonionic surfactants, such as a low or non-foaming nonionic surfactant.
  • a detergent surfactant e.g. anionic or nonionic surfactants, such as a low or non-foaming nonionic surfactant.
  • Such low or non-foaming nonionic surfactants are well-known in the art, and suitable examples can be found in M. Schick "Nonionic Surfactants" Vol. 1, (1967).
  • builder materials may comprise organic and/or inorganic builder materials, usually in amounts of from 10 - 80 %, for most practical purposes from 20 - 60 % by weight.
  • builder materials include alkali metal polyphosphates, -pyrophosphates, -hexametaphosphates, -orthophosphates, -carbonates, -bicarbonates, -borates, -silicates: furthermore, alkali metal polycarboxylates and -polyhydroxysulphonates. Additional examples include alkali metal citrates, -nitrilotriacetates, -carboxymethyloxysuccinates, zeolites etc. Polyelectrolytes such as polymaleates and polyacrylates are also suitable examples.
  • peroxy-type bleaching agents may be included such as alkalimetal perborates, -percarbonates, -persulphates as well as organic peracids and salts thereof.
  • Bleach precursors such as tetraacetylethylenediamine may also be included together with a peroxy type bleaching agents such as sodium perborate.
  • Buffers perfumes, dyes, germicides, solvents, foam depressors, clays such as hectorites, corrosion inhibitors anti-tarnishing agents etc. may also be included if required.
  • a set of 8 glass plates (4 x 4 cm) were soiled with 51.5 ⁇ 1.8 mg baked-on egg yolk per glass plate. These were soaked in 1 liter water of 27 ° GH containing the above amounts of the dishwashing composition for a period of one hour at pH 10.0. After soaking the residual egg-yolk present on the plates was determined by weighing and measuring the weight difference of the soiled plates before and after soaking.
  • the dishwashing composition was dosed in an amount of 30 g, and had the following formulation:
  • the soiling was 6 g egg-yolk.

Claims (2)

1. Enzymatische Geschirrwaschzusammensetzung umfassend 0,5-5 Gew.-% eines Detergens-oberflächenaktiven Materials, 10-80 Gew.-% eines Builders und Proteasen in einer Menge, derart, daß die Zusammensetzung eine proteolytische Aktivität von 0,1-50 Glyzin-Einheiten pro Milligramm aufweist, dadurch gekennzeichnet, daß sie weiterhin 0,005-100 Lipase-Einheiten/mg einer bakteriellen Lipase umfaßt, die eine positive immunologische Kreuzreaktion mit dem Antikörper der Lipase, die durch Chromobacter viscosum var. lipolyticum NRRL B 3673 gebildet wird, zeigt.
2. Zusammensetzung nach Anspruch 1, dadurch gekennzeichnet, daß die Lipase erhältlich ist aus Pseudomonas fluorescens, Pseudomonas fraai. Pseudomonas nitroreducens var. lipolyticum, Pseudomonas cepacia, Pseudomonas gladioli und Chromobacter viscosum.
EP87202396A 1986-12-10 1987-12-02 Enzyme enthaltendes Geschirrwaschmittel Expired - Lifetime EP0271156B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8629537 1986-12-10
GB868629537A GB8629537D0 (en) 1986-12-10 1986-12-10 Enzymatic dishwashing composition

Publications (3)

Publication Number Publication Date
EP0271156A2 EP0271156A2 (de) 1988-06-15
EP0271156A3 EP0271156A3 (en) 1988-08-03
EP0271156B1 true EP0271156B1 (de) 1990-07-18

Family

ID=10608787

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87202396A Expired - Lifetime EP0271156B1 (de) 1986-12-10 1987-12-02 Enzyme enthaltendes Geschirrwaschmittel

Country Status (9)

Country Link
EP (1) EP0271156B1 (de)
JP (1) JPH0647679B2 (de)
AU (1) AU609433B2 (de)
BR (1) BR8706684A (de)
CA (1) CA1288369C (de)
DE (1) DE3763814D1 (de)
ES (1) ES2017711B3 (de)
GB (1) GB8629537D0 (de)
ZA (1) ZA879299B (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987000859A1 (en) * 1985-08-09 1987-02-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
BE1001436A3 (fr) * 1988-02-22 1989-10-31 Synfina Sa Nouvelle lipase et compositions detergentes en contenant.
GB8813687D0 (en) * 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing & rinsing composition
JPH0277499A (ja) * 1988-09-14 1990-03-16 Lion Corp 自動食器洗い機用洗浄剤組成物
US4959179A (en) * 1989-01-30 1990-09-25 Lever Brothers Company Stabilized enzymes liquid detergent composition containing lipase and protease
US5089163A (en) * 1989-01-30 1992-02-18 Lever Brothers Company, Division Of Conopco, Inc. Enzymatic liquid detergent composition
JPH0699714B2 (ja) * 1990-07-11 1994-12-07 花王株式会社 自動食器洗浄機用洗浄剤組成物
JP2986595B2 (ja) * 1991-11-08 1999-12-06 ダイセル化学工業株式会社 新規リパーゼ
CA2088230A1 (en) * 1992-02-03 1993-08-04 James Gordon Detergent composition
NZ293401A (en) * 1994-09-06 1998-02-26 Johnson & Son Inc S C Liquid enzyme-based cleaning composition buffered at ph 10-14 and comprising lipase, amylase, surfactant, alkali metal silicate and optionally protease
JP2010520767A (ja) 2007-03-09 2010-06-17 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン アルカリ性バシルス種（Ｂａｃｉｌｌｕｓｓｐ．）アルファ‐アミラーゼ変異体、アルファ‐アミラーゼ変異体を含む組成物及び使用方法。
DK2291526T3 (da) 2008-06-06 2014-10-06 Danisco Us Inc Saccharificering af enzymsammensætning med bacillus subtilis alpha-amylase
CA2726630A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Production of glucose from starch using alpha-amylases from bacillus subtilis
US8323945B2 (en) 2008-06-06 2012-12-04 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof
MX2011003178A (es) 2008-09-25 2011-04-21 Danisco Inc Mezclas de alfa-amilasa y metodos para usar esas mezclas.
WO2011049945A2 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1619087A1 (de) * 1967-08-14 1969-10-02 Henkel & Cie Gmbh Als Textilwaschmittel brauchbare Tensidkombinationen sowie diese enthaltende Wasch- oder Waschhilfsmittel
GB1273545A (en) * 1968-06-24 1972-05-10 Albright & Wilson Multi-enzyme cleaning compositions
DK289083A (da) * 1983-06-23 1984-12-24 Novo Industri As Lipase, fremgangsmaade til fremstilling deraf og anvendelse deraf
GB8514707D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514708D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (da) * 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
JPS6434560A (en) * 1987-07-30 1989-02-06 Seiko Epson Corp Die structure
JPS6434559A (en) * 1987-07-30 1989-02-06 Seiko Epson Corp Structure for taking out cavity

Also Published As

Publication number Publication date
BR8706684A (pt) 1988-07-19
DE3763814D1 (de) 1990-08-23
ES2017711B3 (es) 1991-03-01
JPH0647679B2 (ja) 1994-06-22
CA1288369C (en) 1991-09-03
EP0271156A3 (en) 1988-08-03
AU8222587A (en) 1988-06-16
ZA879299B (en) 1989-08-30
GB8629537D0 (en) 1987-01-21
EP0271156A2 (de) 1988-06-15
AU609433B2 (en) 1991-05-02
JPS63159498A (ja) 1988-07-02

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