EP0040237A1 - Process for the preparation of active dried microorganisms - Google Patents
Process for the preparation of active dried microorganismsInfo
- Publication number
- EP0040237A1 EP0040237A1 EP80902356A EP80902356A EP0040237A1 EP 0040237 A1 EP0040237 A1 EP 0040237A1 EP 80902356 A EP80902356 A EP 80902356A EP 80902356 A EP80902356 A EP 80902356A EP 0040237 A1 EP0040237 A1 EP 0040237A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- particles
- dried
- yeast
- drying
- process according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims description 29
- 238000002360 preparation method Methods 0.000 title claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 42
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 42
- 238000001035 drying Methods 0.000 claims abstract description 38
- 239000002245 particle Substances 0.000 claims abstract description 24
- 239000004094 surface-active agent Substances 0.000 claims abstract description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 4
- IZHVBANLECCAGF-UHFFFAOYSA-N 2-hydroxy-3-(octadecanoyloxy)propyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)COC(=O)CCCCCCCCCCCCCCCCC IZHVBANLECCAGF-UHFFFAOYSA-N 0.000 claims description 4
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 claims description 4
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- QHZLMUACJMDIAE-SFHVURJKSA-N 1-hexadecanoyl-sn-glycerol Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](O)CO QHZLMUACJMDIAE-SFHVURJKSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- QHZLMUACJMDIAE-UHFFFAOYSA-N Palmitic acid monoglyceride Natural products CCCCCCCCCCCCCCCC(=O)OCC(O)CO QHZLMUACJMDIAE-UHFFFAOYSA-N 0.000 claims description 2
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims description 2
- 239000001530 fumaric acid Substances 0.000 claims description 2
- 235000011087 fumaric acid Nutrition 0.000 claims description 2
- 229940074045 glyceryl distearate Drugs 0.000 claims description 2
- 125000003976 glyceryl group Chemical group [H]C([*])([H])C(O[H])([H])C(O[H])([H])[H] 0.000 claims description 2
- 229940075507 glyceryl monostearate Drugs 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 235000003441 saturated fatty acids Nutrition 0.000 claims description 2
- 150000004671 saturated fatty acids Chemical class 0.000 claims description 2
- 239000001587 sorbitan monostearate Substances 0.000 claims description 2
- 235000011076 sorbitan monostearate Nutrition 0.000 claims description 2
- 229940035048 sorbitan monostearate Drugs 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 2
- ZORQXIQZAOLNGE-UHFFFAOYSA-N 1,1-difluorocyclohexane Chemical compound FC1(F)CCCCC1 ZORQXIQZAOLNGE-UHFFFAOYSA-N 0.000 claims 1
- FKOKUHFZNIUSLW-UHFFFAOYSA-N 2-Hydroxypropyl stearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(C)O FKOKUHFZNIUSLW-UHFFFAOYSA-N 0.000 claims 1
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 claims 1
- 229940093625 propylene glycol monostearate Drugs 0.000 claims 1
- 229940035044 sorbitan monolaurate Drugs 0.000 claims 1
- 239000001593 sorbitan monooleate Substances 0.000 claims 1
- 235000011069 sorbitan monooleate Nutrition 0.000 claims 1
- 229940035049 sorbitan monooleate Drugs 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000009434 installation Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 3
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- RFVNOJDQRGSOEL-UHFFFAOYSA-N 2-hydroxyethyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCO RFVNOJDQRGSOEL-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 229940010556 ammonium phosphate Drugs 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
Definitions
- the invention relates to a process for the preparation of active dried microorganisms, and more particularly of active dried yeast, such as baker's yeast, brewer's yeast, wine yeast and the like.
- active dried yeast such as baker's yeast, brewer's yeast, wine yeast and the like.
- processes for the drying of yeast are known in general.
- the British Patent Specification no.1.132.793 discloses the drying of compressed yeast, i.e. a yeast having a dry matter content of about 30%, in a fluidized bed.
- British Patent Specification no. 1.230.205 discloses a similar process, relating to the preparation of dry baker's yeast of a high protein content.
- compressed mass of microorganisms is meant a mass of microorganisms, obtained by means of fermentation, from which the accompanying liquid has been removed as far as possible by mechanical means, such as filtration, centrifugation etc.
- yeast a so cabled compressed yeast having a dry matter content varying between 25 and 50% and more specifically between 30 and 40%, is obtained in this way.
- Saccharide disclosed in the British Patent Specification no. 763-926, may be suitably applied.
- the process of the present invention characterized by a drying period in the evacuated space of from 2.5 to 40 hours and more usually from 5—20 hours, which is dependent on the desired dry matter content, the expensive preparation of dry air, which should normally be necessary for the final stage of the drying process, is avoided.
- the present process is much more advantageous than in the case, wherein the complete drying process should be carried out in an evacuated space, because for the initial stage of the drying process the use of moist air is still possible and because the vacuo pumps can be much smaller and less energy consuming than would be necessary for the complete drying process under vacuo.
- the presently proposed process is particularly suitable for the preparation of active dried yeast (derived from a Saccharomyces cerevisiae strain), but may also be applied with good results on other types of yeast, such as brewer's yeast, wine yeast and the like or even on other kinds of microorganisms such as dried moulds and the like.
- the obtained mass is disintegrated, preferably by extruding the yeast through a perforated plate to form strands and breaking up the strands to form particles.
- the holes in the plate may have a cross section of 0.5-3 mm and preferably 1.2-1.6 mm, while after drying particles, having sizes of about 0.4-1.5 mm, are obtained.
- the load of the bed may vary from 100-1000 kg/m 2 of floor area.
- a surface active agent may be added to the material to be dried, in an amount of e.g. 0.5—5% and more particularly 1-2% by weight, calculated on dry matter.
- Suitable surface active agents are e.g. esters of saturated or unsaturated fatty acids and an alcohol, such as sorbitan raonolaurate, sorbitan monostearate, sorbitan raonooleate, glyceryl monostearate, glyceryl distearate, glyceryl monopalmitate, propylenej glycol monostearate or glyceryl mono- or drpalmitate or -stearate, wherein free hydroxy groups optionally have been esterified with a suitable acid for consumption, such as citric acid, tartaric acid, fumaric acid, malic acid, lactic acid and the like.
- a suitable acid for consumption such as citric acid, tartaric acid, fumaric acid, malic acid, lactic acid and the like.
- the initial drying until a dry matter content of 75-90% is preferably carried out by the application of a fluidized bed method e.g., as disclosed in the British Patent Specification no. 1.230.205, using drying air or an initial temperature of from 60 to 200oC, and preferably 90-130o0.
- the yeast particles are kept during such a process, on a wet—bulb temperature of at most 45oC, and in general at most 35oC.
- the load of the fluidized bed drying equipment is varying in amounts from 100-1000 kg/m 2 - floor area and preferably 400- 800 kg/m 2 and more preferably 600 kg/m 2 in general.
- the load of the fluidized bed drying equipment is about 600 kg/m 2 floor area, while open air is sucked, having a dew point of 10-30oC and more preferably of 15-25oC, while the air is filtered to obtain dust free air and is heated in a previous step to 90-120oC and more preferably about 110oC.
- the air space velocity in the fluidized bed will normally amount from 0.5—2.5 m/s and more generally 1.2 m/s, while the leaving air is almost saturated with water vapour and the relative humidity decreased in the final stage of drying.
- the air temperature is lowered at the end of the drying process as function of the actual bed temperature, being at most 35oC.
- the active dried microorganism mass obtained according to the first drying step has a dry matter content of from 75-90%.
- the evacuated room which may be applied for the last stage of drying, may consist of a vacuum drum, which may be rotated and whereby water may be circulated with a temperature of 25-45°C through an external jacket.
- the initial vacuum in the rotating vacuum drum is 10-20 mm Hg (1300-2600 Pa) while the pressure further decreases during this drying step to 10-2 mm Hg (1300-250 Pa).
- Biologically active dried microorganisms preferably dried active baker's yeast, prepared by the process of the invention, constitute further features of the invention. The following example illustrates a way in which the present invention can be carried into effect.
- Baker's yeast was prepared in the usual way, using molasses as nutrient. As phosphorus source ammoniumphosphate is used and as nitrogen source urea and ammonium sulphate were added. The obtained yeast showed a nitrogen content of 6.0-9.0% with several experiments, and 7.2% in general, calculated on dry matter.
- the phosphate content was 1.4-3.0% calculated on dry matter and in general 1.8%.
- the yeast was centrifugated and washed and was subsequently processed to a compressed yeast, showing a dry matter content of 30-36% and more preferably 34-35%, according to the method of the "Salz compiler" by means of socalled vacuumfilters.
- the so obtained compressed yeast was, optionally after addition of a surface active agent, extruded through a plate, having holes of a diameter of 1.4 mm, and the so obtained strands (like spaghetti) were broken up and were passed into a fluidized bed drying equipment.
- the load of the fluidized bed drying was 100-1000 kg/m 2 floor area and in general 600 kg/m . Open air showing a dew point of 13-24oC, was sucked. This air was filtered to dust free and heated in a heat exchanger up to 110oC. The space velocity of the air in the fluidized bed amounted from 0.8-2.0 m/s and in general 1.2 m/s. The leaving air was almost saturated with water vapour, while at the end of the drying, the relative humidity decreased. During the final part of this drying step, the air temperature was lowered as function of the actual bed temperature, whereby the bed temperature amounted to at most 35oC. After a drying period of 3 hours, a yeast was obtained, showing a dry matter content of 85-90%. The so obtained yeast particles were transmitted into a rotating vacuum drum for additional drying. The inner wall of the vacuo drum was corrugated in order to increase the heated area.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL7908427 | 1979-11-19 | ||
| NL7908427A NL7908427A (nl) | 1979-11-19 | 1979-11-19 | Werkwijze voor het bereiden van actieve droge micro-organismen. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP0040237A1 true EP0040237A1 (en) | 1981-11-25 |
Family
ID=19834205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP80902356A Withdrawn EP0040237A1 (en) | 1979-11-19 | 1980-11-17 | Process for the preparation of active dried microorganisms |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0040237A1 (OSRAM) |
| BE (1) | BE886235A (OSRAM) |
| ES (1) | ES496921A0 (OSRAM) |
| GR (1) | GR71630B (OSRAM) |
| IT (1) | IT8068771A0 (OSRAM) |
| MA (1) | MA19000A1 (OSRAM) |
| NL (1) | NL7908427A (OSRAM) |
| OA (1) | OA06654A (OSRAM) |
| PT (1) | PT72020B (OSRAM) |
| WO (1) | WO1981001415A1 (OSRAM) |
| ZW (1) | ZW28080A1 (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5451413A (en) * | 1992-09-17 | 1995-09-19 | Gist-Brocades, B.V. | Yeast derivative and method to improve bread quality |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1155422B (it) * | 1982-12-10 | 1987-01-28 | Dox Al Italia Spa | Procedimento per la preparazione di una coltura integrale di cellule di saccharomyces cerevisiae |
| EP0588426B1 (en) * | 1992-09-17 | 2000-12-27 | Dsm N.V. | Yeast derivative to improve bread quality |
| EP0616030A1 (en) | 1993-01-27 | 1994-09-21 | Gist-Brocades N.V. | Instant dry yeast |
| PT1498477E (pt) * | 2003-07-18 | 2007-08-13 | B F L Bakery Future Lines S R | Nova levedura activa na forma granular ou em pó seca obtida através do tratamento de uma mistura de leveduras conhecidas e a sua utilização numa preparação de longa duração pré-embalada a ser reconstituída coma adição de água |
| FR2946985B1 (fr) | 2009-06-19 | 2011-07-01 | Lesaffre & Cie | Levure, procede de fabrication, composition, installation |
| CN111066952A (zh) * | 2019-12-26 | 2020-04-28 | 安琪酵母（伊犁）有限公司 | 耐高温球形酵母颗粒、其制备方法及全价颗粒料 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE512562A (OSRAM) * | ||||
| US2894842A (en) * | 1957-05-10 | 1959-07-14 | Jr Jack H Mitchell | Thermostable active dry yeast compositions |
| US3843800A (en) * | 1968-11-08 | 1974-10-22 | Arend Langejan | Preparation of active dried baker's yeast |
-
1979
- 1979-11-19 NL NL7908427A patent/NL7908427A/nl not_active Application Discontinuation
-
1980
- 1980-11-05 PT PT72020A patent/PT72020B/pt unknown
- 1980-11-13 MA MA19202A patent/MA19000A1/fr unknown
- 1980-11-17 ZW ZW280/80A patent/ZW28080A1/xx unknown
- 1980-11-17 ES ES496921A patent/ES496921A0/es active Granted
- 1980-11-17 WO PCT/NL1980/000038 patent/WO1981001415A1/en not_active Ceased
- 1980-11-17 EP EP80902356A patent/EP0040237A1/en not_active Withdrawn
- 1980-11-18 BE BE0/202855A patent/BE886235A/fr unknown
- 1980-11-18 GR GR63684A patent/GR71630B/el unknown
- 1980-11-19 IT IT8068771A patent/IT8068771A0/it unknown
- 1980-11-19 OA OA57253A patent/OA06654A/xx unknown
Non-Patent Citations (1)
| Title |
|---|
| See references of WO8101415A1 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5451413A (en) * | 1992-09-17 | 1995-09-19 | Gist-Brocades, B.V. | Yeast derivative and method to improve bread quality |
Also Published As
| Publication number | Publication date |
|---|---|
| ES8201625A1 (es) | 1981-12-16 |
| WO1981001415A1 (en) | 1981-05-28 |
| ES496921A0 (es) | 1981-12-16 |
| IT8068771A0 (it) | 1980-11-19 |
| ZW28080A1 (en) | 1981-02-18 |
| PT72020B (en) | 1981-09-24 |
| MA19000A1 (fr) | 1981-07-01 |
| GR71630B (OSRAM) | 1983-06-20 |
| NL7908427A (nl) | 1981-06-16 |
| PT72020A (en) | 1980-12-01 |
| OA06654A (fr) | 1981-09-30 |
| BE886235A (fr) | 1981-05-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| AK | Designated contracting states |
Designated state(s): AT CH DE FR GB LU NL SE |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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Effective date: 19820721 |
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Inventor name: DE BRUIJN, JACOBUS THEODORUS Inventor name: VAN 'T RIET, JOHANNES |