EA201891064A1 - Способ получения осушенных микробных клеток - Google Patents
Способ получения осушенных микробных клетокInfo
- Publication number
- EA201891064A1 EA201891064A1 EA201891064A EA201891064A EA201891064A1 EA 201891064 A1 EA201891064 A1 EA 201891064A1 EA 201891064 A EA201891064 A EA 201891064A EA 201891064 A EA201891064 A EA 201891064A EA 201891064 A1 EA201891064 A1 EA 201891064A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- microbial cells
- obtaining dried
- dried microbial
- obtaining
- viability
- Prior art date
Links
- 230000000813 microbial effect Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 239000003963 antioxidant agent Substances 0.000 abstract 1
- 230000003078 antioxidant effect Effects 0.000 abstract 1
- 239000002738 chelating agent Substances 0.000 abstract 1
- 239000002612 dispersion medium Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 239000003223 protective agent Substances 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Целью настоящего изобретения является разработка способа получения осушенных микробных клеток с высокой степенью жизнеспособности после их длительного хранения при высокой температуре. Способ получения осушенных микробных клеток, который позволяет достичь вышеупомянутой цели, отличается тем, что микробные клетки суспендируют в дисперсионной среде, содержащей протективный агент, антиоксидант и хелатообразующий агент, с последующей сушкой.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015214299 | 2015-10-30 | ||
PCT/JP2016/082125 WO2017073752A1 (ja) | 2015-10-30 | 2016-10-28 | 微生物乾燥菌体の製造方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201891064A1 true EA201891064A1 (ru) | 2019-01-31 |
EA039591B1 EA039591B1 (ru) | 2022-02-15 |
Family
ID=58630294
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201891064A EA039591B1 (ru) | 2015-10-30 | 2016-10-28 | Способ получения осушенных микробных клеток |
Country Status (7)
Country | Link |
---|---|
US (1) | US10844347B2 (ru) |
EP (1) | EP3369805B1 (ru) |
JP (2) | JP6823601B2 (ru) |
CN (1) | CN108271382A (ru) |
EA (1) | EA039591B1 (ru) |
WO (1) | WO2017073752A1 (ru) |
ZA (1) | ZA201802802B (ru) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3648778A4 (en) * | 2017-07-07 | 2021-05-05 | Osel, Inc. | HIGHLY EFFECTIVE STABLE FORMULATIONS OF VAGINAL LACTOBACILLUS |
US20210024878A1 (en) * | 2018-04-05 | 2021-01-28 | Kabushiki Kaisha Yakult Honsha | Method for producing highly viable dried microbial cells |
KR20200001543A (ko) * | 2018-06-26 | 2020-01-06 | 씨제이제일제당 (주) | 구연산염을 포함하는 유산균 동결 보호용 조성물 |
JP7178203B2 (ja) * | 2018-08-07 | 2022-11-25 | 株式会社ヤクルト本社 | 乳酸菌凍結乾燥菌体の製造方法 |
CN109335063A (zh) * | 2018-10-17 | 2019-02-15 | 西北农林科技大学 | 一种微生物固体菌剂的包装方式 |
WO2021193581A1 (ja) * | 2020-03-24 | 2021-09-30 | 三菱ケミカル株式会社 | 排水処理方法 |
WO2022239840A1 (ja) * | 2021-05-14 | 2022-11-17 | 日本曹達株式会社 | 植物保護能を有する微生物の保存 |
CN113603235B (zh) * | 2021-08-18 | 2023-04-21 | 中冶生态环保集团有限公司 | 一种用于ifas/mbbr工艺熟化悬浮载体的保藏方法 |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3897307A (en) | 1974-10-23 | 1975-07-29 | Hansens Lab Inc | Stabilized dry cultures of lactic acid-producing bacteria |
JPS58107181A (ja) * | 1981-12-18 | 1983-06-25 | Nippon Oil Co Ltd | 微生物生菌体の固定化・増殖法 |
JP3504365B2 (ja) * | 1995-02-01 | 2004-03-08 | 森永乳業株式会社 | 微生物保護剤及び該保護剤を用いた凍結又は凍結乾燥微生物の製造法 |
JP3363438B2 (ja) * | 2000-05-02 | 2003-01-08 | ビオフェルミン製薬株式会社 | 噴霧乾燥による菌体乾燥物 |
US6653062B1 (en) | 2000-07-26 | 2003-11-25 | Wisconsin Alumni Research Foundation | Preservation and storage medium for biological materials |
JP4880861B2 (ja) * | 2000-10-13 | 2012-02-22 | 株式会社ヤクルト本社 | 骨代謝改善剤 |
EP2591771A1 (en) | 2002-04-11 | 2013-05-15 | MedImmune, LLC | Preservation of bioactive materials by freeze dried foam |
EP1493806A1 (en) | 2003-07-02 | 2005-01-05 | Chr. Hansen A/S | Use of compounds involved in biosynthesis of nucleic acids as cryoprotective agents |
JP2005325077A (ja) * | 2004-05-17 | 2005-11-24 | Mitsui Chemicals Inc | シュードモナス属細菌の固定化物及びその固定化方法、及びその固定化物から成る、植物の地上部病害防除剤の製剤化方法 |
JP5307801B2 (ja) | 2007-05-18 | 2013-10-02 | メディミューン・エルエルシー | 凍結乾燥フォームによる生物活性材料の防腐 |
JP2010004787A (ja) * | 2008-06-26 | 2010-01-14 | Kaneka Corp | ラクトバチルス・ブレビス菌の培養方法 |
DK2424972T3 (da) | 2009-04-30 | 2013-10-14 | Actogenix Nv | Kryobeskyttende midler til frysetørring af mælkesyrebakterier |
KR101799251B1 (ko) * | 2009-10-22 | 2017-12-12 | 가부시키가이샤 야쿠르트 혼샤 | 유방암 발병 위험 저감제 |
US7888062B1 (en) | 2010-02-01 | 2011-02-15 | Microbios, Inc. | Process and composition for the manufacture of a microbial-based product |
JP2011223990A (ja) * | 2010-03-30 | 2011-11-10 | Nippon Soda Co Ltd | 凍結乾燥菌体の製造方法 |
LT2603100T (lt) | 2010-08-13 | 2018-07-25 | Advanced Bionutrition Corp. | Stabilizuojanti kompozicija, skirta biologinių medžiagų sausam saugojimui |
DK2744888T3 (en) | 2011-08-18 | 2018-03-12 | Chr Hansen As | Process for purifying bacterial cells |
US20130089638A1 (en) | 2011-10-11 | 2013-04-11 | Mead Johnson Nutrition Company | Compositions Comprising Maltotriose And Methods Of Using Same To Inhibit Damage Caused By Dehydration Processes |
CN102559539B (zh) | 2011-12-02 | 2015-04-15 | 北京大北农科技集团股份有限公司 | 嗜酸乳杆菌及其应用与饲料添加剂和预混料 |
CN102978143B (zh) | 2012-12-21 | 2014-04-30 | 哈尔滨工业大学 | 乳杆菌冻干产品及其制备方法 |
-
2016
- 2016-10-28 EP EP16859980.1A patent/EP3369805B1/en active Active
- 2016-10-28 CN CN201680063748.3A patent/CN108271382A/zh active Pending
- 2016-10-28 EA EA201891064A patent/EA039591B1/ru unknown
- 2016-10-28 WO PCT/JP2016/082125 patent/WO2017073752A1/ja active Application Filing
- 2016-10-28 JP JP2017547902A patent/JP6823601B2/ja active Active
- 2016-10-28 US US15/771,728 patent/US10844347B2/en active Active
-
2018
- 2018-04-26 ZA ZA2018/02802A patent/ZA201802802B/en unknown
-
2019
- 2019-10-28 JP JP2019195170A patent/JP6921465B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2020010725A (ja) | 2020-01-23 |
EP3369805A1 (en) | 2018-09-05 |
US10844347B2 (en) | 2020-11-24 |
EP3369805A4 (en) | 2019-06-12 |
CN108271382A (zh) | 2018-07-10 |
JP6823601B2 (ja) | 2021-02-03 |
JP6921465B2 (ja) | 2021-08-18 |
EA039591B1 (ru) | 2022-02-15 |
US20190062693A1 (en) | 2019-02-28 |
WO2017073752A1 (ja) | 2017-05-04 |
JPWO2017073752A1 (ja) | 2018-08-16 |
ZA201802802B (en) | 2019-02-27 |
EP3369805B1 (en) | 2020-08-26 |
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