EA201650048A1 - Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата - Google Patents

Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата

Info

Publication number
EA201650048A1
EA201650048A1 EA201650048A EA201650048A EA201650048A1 EA 201650048 A1 EA201650048 A1 EA 201650048A1 EA 201650048 A EA201650048 A EA 201650048A EA 201650048 A EA201650048 A EA 201650048A EA 201650048 A1 EA201650048 A1 EA 201650048A1
Authority
EA
Eurasian Patent Office
Prior art keywords
conjugate
ethylene
conjugation
links
total number
Prior art date
Application number
EA201650048A
Other languages
English (en)
Other versions
EA030139B1 (ru
Inventor
Аркадий Васильевич НЕКРАСОВ
Темури Мусаевич КАРАПУТАДЗЕ
Сергей Алексеевич МЕДВЕДЕВ
Александр Владимирович КОЗЮКОВ
Нино Темуриевна КАРАПУТАДЗЕ
Original Assignee
ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм" filed Critical ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм"
Publication of EA201650048A1 publication Critical patent/EA201650048A1/ru
Publication of EA030139B1 publication Critical patent/EA030139B1/ru

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/785—Polymers containing nitrogen
    • A61K31/787—Polymers containing nitrogen containing heterocyclic rings having nitrogen as a ring hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/02—Suppositories; Bougies; Bases therefor; Ovules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F26/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C08F26/06—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C08F8/02—Alkylation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C08F8/06—Oxidation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Rheumatology (AREA)
  • Pain & Pain Management (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Medicinal Preparation (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)

Abstract

Изобретение относится к способу получения иммобилизованных ферментных препаратов, в частности к получению и использованию нового активного конъюгата фермента с полимерным носителем. Конъюгат обладает свойствами препарата Лонгидаза и подавляет гиперплазию соединительной ткани, а также обладает противовоспалительным действием и может быть использован для производства стабильных, активных и безопасных в применении лекарственных препаратов пролонгированного действия в форме суппозитория, мази, инъекции или косметического крема, а также для получения ветеринарных лекарственных средств. Способ заключается в конъюгации гиалуронидазы с водорастворимым сополимером карбодимидным или азидным методом. Для конъюгации используют сополимер N-оксида 1,4-этиленпиперазина, (N-карбоксиметилметил)-1,4-этиленпиперазиния или его гидразида, и 1,4-этиленпиперазина общей формулыR=OH, NHNH, где n - от 40 до 90% от общего количества звеньев, m - от 3 до 40% от общего количества звеньев, n+m+1=100%.
EA201650048A 2015-12-04 2016-11-28 Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата EA030139B1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2015152036A RU2616528C1 (ru) 2015-12-04 2015-12-04 Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата

Publications (2)

Publication Number Publication Date
EA201650048A1 true EA201650048A1 (ru) 2017-06-30
EA030139B1 EA030139B1 (ru) 2018-06-29

Family

ID=58642685

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201650048A EA030139B1 (ru) 2015-12-04 2016-11-28 Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата

Country Status (19)

Country Link
US (1) US11364283B2 (ru)
EP (1) EP3384917B1 (ru)
JP (1) JP6683827B2 (ru)
KR (2) KR20200131355A (ru)
CN (1) CN108697732B (ru)
AR (1) AR108562A1 (ru)
CA (1) CA3007389C (ru)
EA (1) EA030139B1 (ru)
GE (1) GEP20207131B (ru)
HK (1) HK1259290A1 (ru)
HU (1) HUE056845T2 (ru)
LT (1) LT3384917T (ru)
MD (1) MD4637C1 (ru)
RS (1) RS62659B1 (ru)
RU (1) RU2616528C1 (ru)
SI (1) SI3384917T1 (ru)
TR (1) TR201807851T1 (ru)
UA (1) UA121425C2 (ru)
WO (1) WO2017095264A1 (ru)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11752212B2 (en) 2017-06-29 2023-09-12 University Of Washington N-oxide and ectoine monomers, polymers, their compositions, and related methods
RU2737271C2 (ru) * 2018-06-27 2020-11-26 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм" Сополимеры ди-N-оксидов поли-1,4-этиленпиперазина и способы их получения
CN111978404A (zh) * 2020-08-11 2020-11-24 西北大学 基于Halo-tag特异性脱卤反应的蛋白质一步定向固定化方法
KR102481458B1 (ko) 2021-12-15 2022-12-27 홍재혁 문신기구

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2801723B2 (ja) * 1990-01-26 1998-09-21 花王株式会社 新規ベタイン及びそれを含有する分散剤
RU2073031C1 (ru) * 1990-08-06 1997-02-10 Некрасов Аркадий Васильевич Производные поли-1,4-этиленпиперазина, обладающие иммуномодулирующей, противовирусной и антибактериальной активностями
HU222189B1 (hu) * 1993-09-10 2003-05-28 Petrovax, Inc. Készítmények bélféregfertőzések megelőzésére és kezelésére
RU2112542C1 (ru) * 1997-02-28 1998-06-10 Аркадий Васильевич Некрасов Препарат для лечения патологических состояний соединительной ткани
RU2185388C2 (ru) * 2000-06-28 2002-07-20 Карапутадзе Темури Мусаевич Способ получения поли-1,4-этиленпиперазина и его производных
EP2662090A1 (en) * 2008-04-14 2013-11-13 Halozyme, Inc. Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions
RU2417097C2 (ru) * 2008-04-24 2011-04-27 Валерия Леонидовна Багирова Фармацевтическая композиция, содержащая гиалуронидазу и липосомы для наружного применения
RU2428991C9 (ru) * 2010-06-24 2011-12-27 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм" Сополимеры гетероцепных алифатических поли-n-оксидов, вакцинирующие и лекарственные средства на их основе
RU2556378C2 (ru) * 2013-07-11 2015-07-10 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НПО Петровакс Фарм" Конъюгат гликопротеина, обладающего активностью эритропоэтина, с производными n-оксида поли-1,4-этиленпиперазина (варианты), фармацевтическая композиция и способ получения конъюгата

Also Published As

Publication number Publication date
KR20180090339A (ko) 2018-08-10
CA3007389C (en) 2021-01-12
MD4637C1 (ru) 2020-01-31
AR108562A1 (es) 2018-09-05
HUE056845T2 (hu) 2022-03-28
MD20180043A2 (ru) 2018-11-30
WO2017095264A1 (ru) 2017-06-08
MD4637B1 (ru) 2019-06-30
EA030139B1 (ru) 2018-06-29
TR201807851T1 (tr) 2018-06-21
CN108697732B (zh) 2021-02-26
JP6683827B2 (ja) 2020-04-22
SI3384917T1 (sl) 2022-01-31
KR20200131355A (ko) 2020-11-23
US11364283B2 (en) 2022-06-21
RS62659B1 (sr) 2021-12-31
US20180344815A1 (en) 2018-12-06
CA3007389A1 (en) 2017-06-08
JP2019501217A (ja) 2019-01-17
UA121425C2 (ru) 2020-05-25
EP3384917B1 (en) 2021-09-15
HK1259290A1 (zh) 2019-11-29
EP3384917A1 (en) 2018-10-10
LT3384917T (lt) 2021-11-25
RU2616528C1 (ru) 2017-04-17
GEP20207131B (en) 2020-07-10
KR102358980B1 (ko) 2022-02-08
CN108697732A (zh) 2018-10-23
EP3384917A4 (en) 2019-09-04

Similar Documents

Publication Publication Date Title
EA201650048A1 (ru) Способ получения конъюгата гиалуронидазы с производными полиэтиленпиперазина и применение полученного конъюгата
CY1120342T1 (el) Ανταγωνιστης πολυκυκλικου lpa1 και χρησεις εξ' αυτου
CO2017011245A2 (es) Conjugados proteína-polisacárido de estreptococo grupo b, métodos para producir conjugados, composiciones inmunógenas que comprenden conjugados y sus usos
CY1124401T1 (el) Τροποποιητες της πρωτεϊνης ρυθμιστη διαμεμβρανικης αγωγιμοτητας κυστικης ινωσης και μεθοδοι χρησης
FR3037958B1 (fr) Nouveaux derives d'hydroxy-acide, leur procede de preparation et les compositions pharmaceutiques qui les contiennent
BR112014016338A2 (pt) polímeros de polioxazolina e processos para a sua preparação, conjugados destes polímeros e usos médicos dos mesmos
PH12017500136A1 (en) Long-chain dimethylaniline derivative compounds, their preparation methods, self-assembled textures, and uses thereof
MX2019011530A (es) Saxitoxinas 11,13-modificadas para el tratamiento del dolor.
WO2019199861A3 (en) Hemp extract for treatment of pain in animals
BR112012017269A2 (pt) compostos heterocíclicos tricíclicos, composições e métodos de uso dos mesmos
MX2020012679A (es) Compuestos de amino-pirazincarboxamida, conjugados, y usos de los mismos.
EA201992162A1 (ru) Составы на основе нирапариба
CR11723A (es) Agente para tratar enfermedad
ATE470443T1 (de) Indol-1-yl-essigsäurederivate
EP4335851A3 (en) Pharmaceutical formulations and methods of use thereof
WO2018201167A3 (en) Propionic acid derivatives and methods of use thereof
EA201691490A1 (ru) Фармацевтическая композиция, содержащая бринзоламид
CY1122892T1 (el) Παραγωγα 3-(καρβοξυμεθυλ)-8-αμινο-2-οξο-1,3-διαζα-σπειρο-[4.5]-δεκανιου
CR20210127A (es) Molécula de unión al antigeno anti grupo de diferenciación 137 (cd137) y su uso
EA201791138A1 (ru) Фармацевтическая композиция, ее приготовление и применения
EA202090014A1 (ru) Гетероароматические соединения в качестве ингибиторов ванина
EA201792516A1 (ru) Макроциклические димеры бензодиазепина, их конъюгаты, получение и применение
EA201790223A1 (ru) Водный препарат, содержащий парацетамол и ибупрофен
EA201690005A1 (ru) Состав с модифицированной кинетикой высвобождения
CY1113885T1 (el) Μια ιατρικη κρεμα φουσιδικου οξεος που παρασκευαζεται με την χρηση φουσιδικου νατριου και ενσωματωση ενος βιοπολυμερους και μια μεθοδος για την παρασκευη της