EA200970985A1 - Система экспрессии - Google Patents

Система экспрессии

Info

Publication number
EA200970985A1
EA200970985A1 EA200970985A EA200970985A EA200970985A1 EA 200970985 A1 EA200970985 A1 EA 200970985A1 EA 200970985 A EA200970985 A EA 200970985A EA 200970985 A EA200970985 A EA 200970985A EA 200970985 A1 EA200970985 A1 EA 200970985A1
Authority
EA
Eurasian Patent Office
Prior art keywords
protein
poi
promoter sequence
relates
pastoris
Prior art date
Application number
EA200970985A
Other languages
English (en)
Other versions
EA017803B1 (ru
Inventor
Бригитте Гассер
Дитхард Маттанович
Михель Зауэр
Герхард Штадльмайр
Original Assignee
Полимун Сайнтифик Иммунбиологише Форшунг Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Полимун Сайнтифик Иммунбиологише Форшунг Гмбх filed Critical Полимун Сайнтифик Иммунбиологише Форшунг Гмбх
Publication of EA200970985A1 publication Critical patent/EA200970985A1/ru
Publication of EA017803B1 publication Critical patent/EA017803B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Данное изобретение относится к способам увеличения секреции представляющего интерес белка (POI) из эукариотической клетки, предусматривающим коэкспрессию POI и по меньшей мере одного белка, который усиливает секрецию белка, причем указанный усиливающий (энхансерный) белок выбран из группы, состоящей из ВМН2, BFR2, COG6, COY1, CUP5, IMH1, KIN2, SEC31, SSA4, SSE1. Это изобретение относится дополнительно к промоторной последовательности дрожжей, в частности промоторной последовательности гена РЕТ9 Р. pastoris, имеющей при сравнимых условиях увеличенную промоторную активность относительно промоторной последовательности белка GAP. Кроме того, это изобретение относится к экспрессирующему вектору, содержащему такую промоторную последовательность, и к применению такого экспрессируюшего вектора для экспрессии POI в клетке-хозяине. Это изобретение, относится также к новым промоторным последовательностям генов из Р. pastoris, которые применимы для экспрессии POI в дрожжах.
EA200970985A 2007-04-20 2008-04-17 Система экспрессии EA017803B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008051 2007-04-20
PCT/EP2008/003076 WO2008128701A2 (en) 2007-04-20 2008-04-17 Yeast expression systems

Publications (2)

Publication Number Publication Date
EA200970985A1 true EA200970985A1 (ru) 2010-04-30
EA017803B1 EA017803B1 (ru) 2013-03-29

Family

ID=39800700

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200970985A EA017803B1 (ru) 2007-04-20 2008-04-17 Система экспрессии

Country Status (10)

Country Link
US (1) US20100297738A1 (ru)
EP (1) EP2140008A2 (ru)
JP (1) JP2010524440A (ru)
KR (1) KR20100016170A (ru)
CN (1) CN101679992A (ru)
AU (1) AU2008241061A1 (ru)
BR (1) BRPI0810357A2 (ru)
CA (1) CA2684650A1 (ru)
EA (1) EA017803B1 (ru)
WO (1) WO2008128701A2 (ru)

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* Cited by examiner, † Cited by third party
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EP2258854A1 (en) * 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012109220A2 (en) * 2011-02-08 2012-08-16 Merck Sharp & Dohme Corp. Cell cycle control for improving process performance and recombinant expression in fungal host cells
ES2913825T3 (es) 2011-10-07 2022-06-06 Lonza Ag Promotor regulable
ES2610990T3 (es) 2012-10-29 2017-05-04 Lonza Ltd Secuencias de expresión
CN102977206B (zh) * 2012-11-19 2014-10-01 中国农业科学院生物技术研究所 细胞色素结合结构域蛋白作为助分泌因子提高外源基因在毕赤酵母中分泌表达量的用途
CN102994541B (zh) * 2012-12-19 2015-04-15 江南大学 一种共表达upr关键基因和下游靶基因增强葡萄糖氧化酶分泌的方法
EP3527667B1 (en) 2013-03-08 2020-11-25 Biogrammatics, Inc. Yeast promoters for protein expression
EP2964765B1 (en) 2013-03-08 2019-05-08 Keck Graduate Institute of Applied Life Sciences Yeast promoters from pichia pastoris
BR112016023304A2 (pt) * 2014-04-17 2017-10-17 Boehringer Ingelheim Rcv Gmbh célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares
KR102291978B1 (ko) 2014-04-17 2021-08-23 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 목적 단백질의 발현을 위한 재조합 숙주세포
EP2952584A1 (en) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Improved protein production
CN104357416A (zh) * 2014-10-22 2015-02-18 江南大学 一种改造蛋白折叠分泌途径增强葡萄糖氧化酶分泌的方法
RU2683549C1 (ru) * 2015-12-29 2019-03-28 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) СИСТЕМА ДЛЯ ЭКСПРЕССИИ FAB-ФРАГМЕНТОВ АНТИТЕЛ В МЕТИЛОТРОФНЫХ ДРОЖЖАХ PICHIAPASTORIS, НА ОСНОВЕ РЕКОМБИНАНТНЫХ ПЛАЗМИДНЫХ ДНК Ab-HCh-HIS/pPICZ_α_A И Ab-LCh-LAMBDA/pPICZα_A, ПРЕДНАЗНАЧЕННЫХ ДЛЯ КЛОНИРОВАНИЯ ВАРИАБЕЛЬНЫХ ДОМЕНОВ ТЯЖЕЛОЙ И ЛЕГКОЙ ЦЕПЕЙ АНТИТЕЛ, СООТВЕТСТВЕННО
CN105802867B (zh) * 2016-05-23 2019-09-17 江南大学 一种碱性果胶酶分泌增强型菌株及其应用
SG11201908079SA (en) 2017-03-29 2019-10-30 Boehringer Ingelheim Rcv Gmbh Recombinant host cell with altered membrane lipid composition
US20210285062A1 (en) * 2017-05-16 2021-09-16 The Regents Of The University Of California Fluorescence detection in yeast colonies
CA3072134A1 (en) 2017-05-31 2018-12-06 Universitat Fur Bodenkultur Wien Yeast expressing a synthetic calvin cycle
CN107043757B (zh) * 2017-06-01 2020-07-07 江苏师范大学 一株异源高效表达米黑根毛霉脂肪酶的重组毕赤酵母及其应用
KR20200120908A (ko) 2018-02-12 2020-10-22 론자 리미티드 목적 단백질을 생산하기 위한 숙주 세포
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
CA3103988A1 (en) * 2018-06-27 2020-01-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of transcription factors
CN113056554A (zh) * 2018-11-19 2021-06-29 巴斯夫欧洲公司 重组酵母细胞
CN111378681B (zh) * 2018-12-27 2023-01-17 中国医学科学院药物研究所 生产达玛烯二醇-ii糖苷的重组菌及其应用
ES2921137T3 (es) 2019-01-11 2022-08-18 Lonza Ag Producción de proteínas regulada por fuente de carbono en una célula huésped recombinante
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
CN114026239A (zh) 2019-04-01 2022-02-08 维也纳自然资源与生命科学大学 Mut-甲醇营养型酵母
CN114341358B (zh) * 2019-07-25 2024-06-14 科学与工业研究委员会 用于在酵母中高表达蛋白质的重组载体
CN110592090A (zh) * 2019-10-30 2019-12-20 福建师范大学 Ssa4基因启动子及利用该启动子驱动外源基因转录的毕赤酵母表达载体
RU2728033C1 (ru) * 2019-12-11 2020-07-28 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт"-ГосНИИгенетика) Трансформант дрожжей Pichia pastoris, продуцирующий эндо-1,4-β-ксиланазу из Paenibacillus brasilensis
WO2021198431A1 (en) 2020-04-01 2021-10-07 Lonza Ltd Helper factors for expressing proteins in yeast
CN116490517A (zh) 2020-09-30 2023-07-25 龙沙有限公司 过表达翻译因子的宿主细胞
CN112280700B (zh) * 2020-10-19 2022-09-06 中国石油化工股份有限公司 一株耐乙酸和甲酸的发酵菌株及其构建方法
KR20230144629A (ko) 2021-02-12 2023-10-16 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 단백질 분비 증가를 위한 신호 펩타이드
CN113088533B (zh) * 2021-04-15 2023-03-24 华中科技大学 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114657197B (zh) * 2022-04-06 2023-07-21 暨南大学 Gsm1p作为正调控因子在提高宿主细胞中蛋白表达中的应用
WO2024126811A1 (en) 2022-12-16 2024-06-20 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of a combination of transport proteins and either chaperones or transcription factors
CN116970503A (zh) * 2023-07-25 2023-10-31 江南大学 一种强化囊泡转运的产乳铁蛋白毕赤酵母及促进胞外分泌的方法
CN117467695B (zh) * 2023-12-27 2024-05-03 南京鸿瑞杰生物医疗科技有限公司 过表达毕赤酵母分子伴侣提高报告蛋白分泌的方法

Also Published As

Publication number Publication date
EA017803B1 (ru) 2013-03-29
EP2140008A2 (en) 2010-01-06
BRPI0810357A2 (pt) 2014-10-07
CN101679992A (zh) 2010-03-24
KR20100016170A (ko) 2010-02-12
JP2010524440A (ja) 2010-07-22
WO2008128701A3 (en) 2009-03-12
AU2008241061A1 (en) 2008-10-30
WO2008128701A2 (en) 2008-10-30
CA2684650A1 (en) 2008-10-30
US20100297738A1 (en) 2010-11-25

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

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