EA200701455A1 - Система для обнаружения флуоресценции - Google Patents

Система для обнаружения флуоресценции

Info

Publication number
EA200701455A1
EA200701455A1 EA200701455A EA200701455A EA200701455A1 EA 200701455 A1 EA200701455 A1 EA 200701455A1 EA 200701455 A EA200701455 A EA 200701455A EA 200701455 A EA200701455 A EA 200701455A EA 200701455 A1 EA200701455 A1 EA 200701455A1
Authority
EA
Eurasian Patent Office
Prior art keywords
samples
fluorescence
photodetector
excitation source
detection system
Prior art date
Application number
EA200701455A
Other languages
English (en)
Other versions
EA013886B1 (ru
Inventor
Уильям Д. Дж. Бикмор
Дэнверн Рэй Робертс
Original Assignee
ЭДВАНСТ МОЛЕКУЛАР СИСТЕМС, ЭлЭлСи
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ЭДВАНСТ МОЛЕКУЛАР СИСТЕМС, ЭлЭлСи filed Critical ЭДВАНСТ МОЛЕКУЛАР СИСТЕМС, ЭлЭлСи
Publication of EA200701455A1 publication Critical patent/EA200701455A1/ru
Publication of EA013886B1 publication Critical patent/EA013886B1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Описаны устройство и способы для возбуждения и обнаружения флуоресценции в образцах. Один из вариантов осуществления устройства включает держатель для удержания образцов, оптический манифольд, содержащий источник возбуждения и/или фотоприемник для каждого из образцов. В другом варианте осуществления оптический манифольд содержит только источник возбуждения или только фотоприемник, соответственно закрепленный на держателе образца. Данная система обеспечивает быстрое возбуждение и измерение флуоресценции без применения подвижных частей и без оптомеханических или электронных помех. Данная система демонстрирует исключительно хорошее отношение сигнал/шум, которое позволяет ей регистрировать очень малые различия в уровнях флуоресценции.
EA200701455A 2005-01-07 2005-12-20 Система для обнаружения флуоресценции EA013886B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/031,526 US7315376B2 (en) 2005-01-07 2005-01-07 Fluorescence detection system
PCT/US2005/046203 WO2006073811A2 (en) 2005-01-07 2005-12-20 Fluorescence detection system

Publications (2)

Publication Number Publication Date
EA200701455A1 true EA200701455A1 (ru) 2008-02-28
EA013886B1 EA013886B1 (ru) 2010-08-30

Family

ID=36647986

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200701455A EA013886B1 (ru) 2005-01-07 2005-12-20 Система для обнаружения флуоресценции

Country Status (11)

Country Link
US (1) US7315376B2 (ru)
EP (2) EP2790010A1 (ru)
JP (1) JP2008536092A (ru)
CN (1) CN101166966B (ru)
AU (1) AU2005323160A1 (ru)
BR (1) BRPI0518510A2 (ru)
CA (1) CA2593814A1 (ru)
EA (1) EA013886B1 (ru)
MX (1) MX2007008315A (ru)
WO (1) WO2006073811A2 (ru)
ZA (1) ZA200705549B (ru)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2407243B1 (en) 2003-07-31 2020-04-22 Handylab, Inc. Multilayered microfluidic device
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP4812393B2 (ja) * 2005-03-04 2011-11-09 株式会社日立ハイテクノロジーズ 蛍光分子計測システム
US7504219B2 (en) * 2005-05-13 2009-03-17 Dxna Llc Methods and apparatus for amplification of DNA using sonic energy
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2016415B1 (en) 2006-04-21 2013-08-14 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods for drug discovery, including discovery of anticancer and antiviral agents
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
US7939312B2 (en) 2006-08-30 2011-05-10 Dxna Llc Rapid thermocycler with movable cooling assembly
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8765076B2 (en) 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
US8153064B2 (en) * 2007-03-22 2012-04-10 Doebler Ii Robert W Systems and devices for isothermal biochemical reactions and/or analysis
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
JP5651011B2 (ja) 2007-07-13 2015-01-07 ハンディーラブ インコーポレイテッド ポリヌクレオチド捕捉材料およびその使用方法
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
WO2009089466A2 (en) 2008-01-09 2009-07-16 Keck Graduate Institute System, apparatus and method for material preparation and/or handling
WO2010101926A2 (en) * 2009-03-02 2010-09-10 The Johns Hopkins University Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption
WO2010115160A2 (en) * 2009-04-03 2010-10-07 Helixis, Inc. Devices and methods for heating biological samples
US9260475B2 (en) * 2009-06-26 2016-02-16 Claremont Biosolutions Llc Capture and elution of bio-analytes via beads that are used to disrupt specimens
TWI397679B (zh) * 2009-07-30 2013-06-01 Ind Tech Res Inst 螢光感測裝置及方法
US8987685B2 (en) * 2009-09-09 2015-03-24 Pcr Max Limited Optical system for multiple reactions
EP2752245A3 (en) * 2010-07-23 2016-08-24 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
US9816131B2 (en) 2010-08-02 2017-11-14 Dxna Llc Pressurizable cartridge for polymerase chain reactions
IT1403792B1 (it) * 2010-12-30 2013-10-31 St Microelectronics Srl Analizzatore per analisi biochimiche e metodo per la determinazione di concentrazioni di sostanze fluorescenti in una soluzione
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
EP2605001A1 (en) * 2011-12-15 2013-06-19 Hain Lifescience GmbH A device and method for optically measuring fluorescence of nucleic acids in test samples and use of the device and method
WO2013116769A1 (en) 2012-02-03 2013-08-08 Becton, Dickson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
MX2015012031A (es) 2013-03-15 2016-03-08 Nanobiosym Inc Sistemas y métodos para el análisis con dispositivo móvil de ácidos nucleicos y proteínas.
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
US20140373643A1 (en) * 2013-06-25 2014-12-25 Dale D. Timm, Jr. Internally illuminated heating and/or chilling bath
EP3014251A1 (en) 2013-06-28 2016-05-04 Streck Inc. Devices for real-time polymerase chain reaction
CA2926253A1 (en) 2013-10-07 2015-04-16 Douglas Scientific, LLC Portable testing device for analyzing biological samples
EP3058349B1 (de) * 2013-10-14 2021-04-21 OASE GmbH Messvorrichtung
CN103969236A (zh) * 2014-05-07 2014-08-06 华中科技大学 一种便携式病原体检测仪
JP6496416B2 (ja) * 2015-02-06 2019-04-03 ライフ テクノロジーズ コーポレーション 純粋な染料計器を正常化する方法およびシステム
SG11201706329WA (en) * 2015-02-06 2017-09-28 Life Technologies Corp Methods and systems for determining optical regions of interest
RU2719346C2 (ru) * 2015-02-06 2020-04-17 Лайф Текнолоджиз Корпорейшн Способы и системы для экспериментального планирования и анализа
CN107817227B (zh) 2016-09-12 2020-08-28 台达电子国际（新加坡）私人有限公司 荧光检测装置
EP3312593A1 (de) * 2016-10-20 2018-04-25 Hain Lifescience GmbH Verfahren und vorrichtung zur optischen anregung einer mehrzahl von analyten in einem array von reaktionsgefässen und zur erfassung von fluoreszenzlicht aus dem analyten
SG10201609334WA (en) 2016-11-08 2018-06-28 Delta Electronics Intl Singapore Pte Ltd Multi-Channel Fluorescence Detection Device
WO2018136797A1 (en) * 2017-01-19 2018-07-26 Indevr, Inc. Parallel imaging system
SG10201801853WA (en) 2018-03-02 2019-10-30 Delta Electronics Int’L Singapore Pte Ltd Portable multi-color fluorescence detection device
TWI722785B (zh) 2020-01-31 2021-03-21 台達電子工業股份有限公司 光學校正工具

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57501048A (ru) * 1980-07-24 1982-06-10
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
JP2779824B2 (ja) * 1989-02-09 1998-07-23 スズキ株式会社 免疫学的凝集反応検出装置
US5073029A (en) * 1990-02-16 1991-12-17 Eqm Research, Inc. Multisource device for photometric analysis and associated chromogens
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US6399397B1 (en) * 1992-09-14 2002-06-04 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2159830C (en) * 1994-04-29 2001-07-03 Timothy M Woudenberg System for real time detection of nucleic acid amplification products
EP0736767A1 (de) * 1995-04-07 1996-10-09 Ciba-Geigy Ag Optische Detektionsvorrichtung für analytische Messungen an chemischen Substanzen
US5935524A (en) * 1996-05-07 1999-08-10 E. I. Du Pont De Nemours And Company Holder for fluorometric samples
US6084683A (en) * 1996-05-28 2000-07-04 Bruno; Alfredo Emilio Optical detection apparatus for chemical analyses of small volumes of samples
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US5854684A (en) * 1996-09-26 1998-12-29 Sarnoff Corporation Massively parallel detection
US6597450B1 (en) * 1997-09-15 2003-07-22 Becton, Dickinson And Company Automated Optical Reader for Nucleic Acid Assays
GB9719673D0 (en) * 1997-09-17 1997-11-19 Glaxo Group Ltd Novel apparatus
US6369893B1 (en) * 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
US6027695A (en) * 1998-04-01 2000-02-22 Dupont Pharmaceuticals Company Apparatus for holding small volumes of liquids
US6496260B1 (en) * 1998-12-23 2002-12-17 Molecular Devices Corp. Vertical-beam photometer for determination of light absorption pathlength
US6838680B2 (en) * 1999-05-12 2005-01-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
US7119345B2 (en) * 2003-02-28 2006-10-10 Applera Corporation Excitation and emission filter
US6852986B1 (en) * 1999-11-12 2005-02-08 E. I. Du Pont De Nemours And Company Fluorometer with low heat-generating light source
DE19962779B4 (de) * 1999-12-23 2009-06-25 Byk-Gardner Gmbh Vorrichtung zur quantifizierten Bestimmung der Qualität von Oberflächen
DE10058579A1 (de) * 2000-11-18 2002-06-13 Sentronic Gmbh Ges Fuer Optisc Vorrichtung und Verfahren zur optischen Messung von Konzentrationen eines Stoffes
EP1385627A1 (en) * 2001-04-09 2004-02-04 David P. Dumas Transparent polymer support for organic synthesis
JP4569030B2 (ja) * 2001-04-23 2010-10-27 東ソー株式会社 外部光下で計測可能な蛍光検出方法、および装置
US20020182111A1 (en) * 2001-06-02 2002-12-05 Ilya Feygin Method and apparatus for visible spectrum imaging
JP4596690B2 (ja) * 2001-06-25 2010-12-08 日本電産コパル株式会社 紙葉類蛍光検出センサ
JP2003042956A (ja) * 2001-07-31 2003-02-13 Fuji Photo Film Co Ltd データの読み取り方法およびそれに用いるスキャナ
JP2005502895A (ja) * 2001-09-19 2005-01-27 ジュール マイクロシステムズ カナダ インコーポレイテッド 信号整合フィルタリングを組み込んだ分光計
US7218810B2 (en) * 2001-09-27 2007-05-15 Bio-Rad Laboratories, Inc. Biochemical assay detection in a liquid receptacle using a fiber optic exciter
AU2003245302A1 (en) * 2002-05-17 2003-12-02 Applera Corporation Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
US7285424B2 (en) * 2002-08-27 2007-10-23 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
US6897954B2 (en) * 2002-12-20 2005-05-24 Becton, Dickinson And Company Instrument setup system for a fluorescence analyzer
US6912050B2 (en) * 2003-02-03 2005-06-28 Hach Company Phase shift measurement for luminescent light
US7122799B2 (en) * 2003-12-18 2006-10-17 Palo Alto Research Center Incorporated LED or laser enabled real-time PCR system and spectrophotometer
JP5007440B2 (ja) * 2004-05-13 2012-08-22 ナノバイオシン， インコーポレイテッド ナノ−ｐｃｒ：核酸増幅及び検出のための方法及び装置

Also Published As

Publication number Publication date
EP1844320A4 (en) 2010-03-03
US7315376B2 (en) 2008-01-01
EP1844320B1 (en) 2014-06-04
WO2006073811A2 (en) 2006-07-13
ZA200705549B (en) 2008-10-29
AU2005323160A1 (en) 2006-07-13
JP2008536092A (ja) 2008-09-04
WO2006073811A3 (en) 2007-11-01
US20060152727A1 (en) 2006-07-13
CN101166966B (zh) 2011-11-09
EA013886B1 (ru) 2010-08-30
BRPI0518510A2 (pt) 2008-11-25
MX2007008315A (es) 2007-10-23
EP1844320A2 (en) 2007-10-17
CN101166966A (zh) 2008-04-23
CA2593814A1 (en) 2006-07-13
EP2790010A1 (en) 2014-10-15

Similar Documents

Publication Publication Date Title
EA200701455A1 (ru) Система для обнаружения флуоресценции
DK2082239T3 (da) Sekventiel analyse af biologiske prøver med intermediær blegning af fluorescerensdetektor
ATE398769T1 (de) Vorrichtung und verfahren zum fluoreszenznachweis in biologischen proben
EP1962085A3 (en) Apparatus for emitting and detecting beams of light
SE0800724L (sv) Blodviskositetsanalys
SE0601563L (sv) Räkning av trombocyter
EP2078952A3 (en) Rapid particle detection assay
DK2132339T3 (da) Fremgangsmåde til detektering af en analyt i en biologisk matrix
DE602004028445D1 (de) Lichtdurchlässige festmatrix-prüfvorrichtung zur mikroarray-analyse
ATE357653T1 (de) Fluoreszenzabbildung mittels telezentrischer anregungs- und abbildungsoptiken
ATE359500T1 (de) Fluoreszenzabbildung mittels telezentrizität
EP2340301A4 (en) IMAGING ANALYZER FOR ANALYTE TESTING
DE10190978D2 (de) Vorrichtung und Verfahren zum Nachweis organischer Moleküle in einer Probensubstanz
ATE541216T1 (de) Fluoreszenztestverfahren
DE60326336D1 (de) Vorrichtung und verfahren um gleichzeitig verschiedene antikörper und antigene nachzuweisen in klinischen, nahrungsmittel- und umwelt-proben
ATE412772T1 (de) Fluoreszenzspektroskopie in absorbierenden medien
DK2155185T3 (da) Påvisning af succinylacetone
DE60315715D1 (de) Verwendung von nicotinamide n-methyltransferase zur diagnose von kolorektalem krebs
TW201520534A (zh) 螢光試紙、螢光激發裝置以及可攜式螢光分析系統
NO20003709L (no) Fremgangsmate for detektering av en analytt, og omsluttingsmiddel og kit for anvendelse deri
WO2010048277A3 (en) Optical auto-calibration method
DK1275963T3 (da) Testapparat med volumenmåler
EP1857805A3 (en) Apparatus for analyzing particles in urine and method thereof
CN103185696B (zh) 一种固体样品载物台、分光光度计及lspr检测方法
ATE393393T1 (de) Verwendung des proteins spee als marker für brustkrebs

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU