EA200200428A1 - Способ секвенирования днк - Google Patents

Способ секвенирования днк

Info

Publication number
EA200200428A1
EA200200428A1 EA200200428A EA200200428A EA200200428A1 EA 200200428 A1 EA200200428 A1 EA 200200428A1 EA 200200428 A EA200200428 A EA 200200428A EA 200200428 A EA200200428 A EA 200200428A EA 200200428 A1 EA200200428 A1 EA 200200428A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dna sequencing
sequencing method
polynucleotide
enzyme
detection
Prior art date
Application number
EA200200428A
Other languages
English (en)
Other versions
EA006702B1 (ru
Inventor
Дэниэл Генри Деншэм
Original Assignee
Медикал Биосистемз Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Медикал Биосистемз Лтд. filed Critical Медикал Биосистемз Лтд.
Publication of EA200200428A1 publication Critical patent/EA200200428A1/ru
Publication of EA006702B1 publication Critical patent/EA006702B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Описывается способ определения последовательности полинуклеотида, причем способ основан на обнаружении конформационного изменения в ферменте, который взаимодействует с полинуклеотидом и передвигается по нему с проявлением своего действия. Обнаружение конформационного изменения можно осуществить, измеряя изменения во флуорофоре, связанном с ферментом.Отчет о международном поиске был опубликован 2002.05.16.
EA200200428A 1999-10-06 2000-10-06 Способ определения последовательности полинуклеотида EA006702B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9923644.0A GB9923644D0 (en) 1999-10-06 1999-10-06 DNA sequencing
PCT/GB2000/003860 WO2001025480A2 (en) 1999-10-06 2000-10-06 Dna sequencing method

Publications (2)

Publication Number Publication Date
EA200200428A1 true EA200200428A1 (ru) 2002-10-31
EA006702B1 EA006702B1 (ru) 2006-02-24

Family

ID=10862234

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200200428A EA006702B1 (ru) 1999-10-06 2000-10-06 Способ определения последовательности полинуклеотида

Country Status (21)

Country Link
US (4) US20050214849A1 (ru)
EP (1) EP1226271B1 (ru)
JP (2) JP5030249B2 (ru)
KR (1) KR100809171B1 (ru)
CN (1) CN1159457C (ru)
AT (1) ATE388242T1 (ru)
AU (1) AU769102B2 (ru)
BR (1) BR0014468A (ru)
CA (1) CA2386115C (ru)
DE (1) DE60038244T2 (ru)
DK (1) DK1226271T3 (ru)
EA (1) EA006702B1 (ru)
ES (1) ES2302497T3 (ru)
GB (1) GB9923644D0 (ru)
HK (1) HK1045857A1 (ru)
IL (2) IL148678A0 (ru)
IS (1) IS6333A (ru)
MX (1) MXPA02003534A (ru)
NZ (1) NZ517775A (ru)
PT (1) PT1226271E (ru)
WO (1) WO2001025480A2 (ru)

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CN102317471A (zh) * 2008-12-11 2012-01-11 加利福尼亚大学董事会 用于单个dna分子的直接测序的方法和系统
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GB201306444D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
EP3828279B1 (en) 2014-07-15 2022-11-16 Illumina, Inc. Biochemically activated electronic device
WO2016106286A1 (en) 2014-12-23 2016-06-30 Biodesy, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
US10294514B2 (en) 2016-04-29 2019-05-21 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
CA3033741A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Method and system for sequencing nucleic acids
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP3562962B1 (en) 2016-12-30 2022-01-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
NZ759548A (en) * 2017-09-15 2024-05-31 Illumina Inc Sequence-detection system
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US11499962B2 (en) 2017-11-17 2022-11-15 Ultima Genomics, Inc. Methods and systems for analyte detection and analysis
US10267790B1 (en) 2017-11-17 2019-04-23 Ultima Genomics, Inc. Systems for biological sample processing and analysis
US10512911B1 (en) * 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
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Also Published As

Publication number Publication date
IL148678A0 (en) 2002-09-12
IS6333A (is) 2002-04-05
WO2001025480A2 (en) 2001-04-12
ES2302497T3 (es) 2008-07-16
IL148678A (en) 2008-12-29
EA006702B1 (ru) 2006-02-24
DE60038244D1 (de) 2008-04-17
CN1159457C (zh) 2004-07-28
KR100809171B1 (ko) 2008-02-29
ATE388242T1 (de) 2008-03-15
US20050214849A1 (en) 2005-09-29
KR20020059444A (ko) 2002-07-12
MXPA02003534A (es) 2004-09-10
WO2001025480A3 (en) 2002-05-16
US7939264B1 (en) 2011-05-10
JP2003511043A (ja) 2003-03-25
EP1226271B1 (en) 2008-03-05
BR0014468A (pt) 2002-08-20
AU769102B2 (en) 2004-01-15
US20120214164A1 (en) 2012-08-23
AU7546700A (en) 2001-05-10
HK1045857A1 (en) 2002-12-13
DE60038244T2 (de) 2009-04-23
CN1391615A (zh) 2003-01-15
DK1226271T3 (da) 2008-07-07
NZ517775A (en) 2004-01-30
US20110177520A1 (en) 2011-07-21
CA2386115C (en) 2010-06-15
JP5030249B2 (ja) 2012-09-19
EP1226271A2 (en) 2002-07-31
GB9923644D0 (en) 1999-12-08
JP2012196201A (ja) 2012-10-18
CA2386115A1 (en) 2001-04-12
PT1226271E (pt) 2008-05-23

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU