EA200101015A1 - СПОСОБ МИКРОБИОЛОГИЧЕСКОГО ПОЛУЧЕНИЯ α-L-АСПАРИЛ-L-ФЕНИЛАЛАНИНА - Google Patents
СПОСОБ МИКРОБИОЛОГИЧЕСКОГО ПОЛУЧЕНИЯ α-L-АСПАРИЛ-L-ФЕНИЛАЛАНИНАInfo
- Publication number
- EA200101015A1 EA200101015A1 EA200101015A EA200101015A EA200101015A1 EA 200101015 A1 EA200101015 A1 EA 200101015A1 EA 200101015 A EA200101015 A EA 200101015A EA 200101015 A EA200101015 A EA 200101015A EA 200101015 A1 EA200101015 A1 EA 200101015A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- asp
- phe
- new
- phenylalanine
- domain
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Настоящее изобретение относится к новому микробиологическому способу получения α-L-аспартил-L-фенилаланина (Asp-Phe) из субстратов L-аспарагиновой кислоты (L-Asp) и L-фенилаланина (L-Phe), в котором субстраты контактируют в присутствии АТФ с нерибосомальной дипептидсинтетазой, включающей два минимальных модуля, соединенных одним доменом конденсации, где N- и С-концевой модули узнают, соответственно, L-Asp и L-Phe, где последний модуль ковалентно связан на своем N-конце с доменом конденсации, и где каждый из данных минимальных модулей состоит из домена аденилирования и содержащего 4'-фосфопантетеинильный кофактор домена тиолирования, а образующийся Asp-Phe извлекают. Настоящее изобретение также относится к новым фрагментам ДНК или сочетанию фрагментов ДНК, кодирующим новую дипептид (Asp-Phe)-синтетазу, к микроорганизмам, содержащим такие фрагменты ДНК, а также к самим новым дипептид (Asp-Phe)-синтетазам.Международная заявка была опубликована вместе с отчетом о международном поиске.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99200954 | 1999-03-29 | ||
| EP99203518A EP1096011A1 (en) | 1999-05-10 | 1999-10-26 | Microbiological production method for alpha-l-aspartyl-l-phenylalanine |
| PCT/NL2000/000206 WO2000058478A1 (en) | 1999-03-29 | 2000-03-28 | MICROBIOLOGICAL PRODUCTION METHOD FOR α-L-ASPARTYL-L-PHENYLALANINE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA200101015A1 true EA200101015A1 (ru) | 2002-02-28 |
Family
ID=26153289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200101015A EA200101015A1 (ru) | 1999-03-29 | 2000-03-28 | СПОСОБ МИКРОБИОЛОГИЧЕСКОГО ПОЛУЧЕНИЯ α-L-АСПАРИЛ-L-ФЕНИЛАЛАНИНА |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1165799A1 (ru) |
| JP (1) | JP2002539835A (ru) |
| KR (1) | KR20010112931A (ru) |
| CN (1) | CN1353757A (ru) |
| AU (1) | AU3843900A (ru) |
| BR (1) | BR0010398A (ru) |
| CA (1) | CA2365594A1 (ru) |
| EA (1) | EA200101015A1 (ru) |
| MX (1) | MXPA01009875A (ru) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100362109C (zh) * | 2003-01-24 | 2008-01-16 | 味之素株式会社 | α-L-天冬氨酰-L-苯丙氨酸-β-酯的生产方法和α-L-天冬氨酰-L-苯丙氨酸-α-甲酯的生产方法 |
| JP4500978B2 (ja) * | 2003-01-24 | 2010-07-14 | 味の素株式会社 | α−Ｌ−アスパルチル−Ｌ−フェニルアラニン−β−エステルの製造方法およびα−Ｌ−アスパルチル−Ｌ−フェニルアラニン−α−メチルエステルの製造方法 |
| JP2005040037A (ja) * | 2003-07-25 | 2005-02-17 | Ajinomoto Co Inc | ジペプチドの製造方法、それに用いるペプチド生成酵素、およびペプチド生成酵素の製造方法 |
| US20050106703A1 (en) * | 2003-11-05 | 2005-05-19 | Kyowa Hakko Kogyo Co., Ltd. | Microorganisms producing dipeptides and process for producing dipeptides using the microorganisms |
| JP5424530B2 (ja) * | 2004-06-25 | 2014-02-26 | 協和発酵バイオ株式会社 | ジペプチドまたはジペプチド誘導体の製造法 |
| JP5424531B2 (ja) * | 2004-06-25 | 2014-02-26 | 協和発酵バイオ株式会社 | ジペプチドまたはジペプチド誘導体の製造法 |
-
2000
- 2000-03-28 BR BR0010398-5A patent/BR0010398A/pt not_active IP Right Cessation
- 2000-03-28 EA EA200101015A patent/EA200101015A1/ru unknown
- 2000-03-28 AU AU38439/00A patent/AU3843900A/en not_active Abandoned
- 2000-03-28 CN CN00808250A patent/CN1353757A/zh active Pending
- 2000-03-28 EP EP00917475A patent/EP1165799A1/en not_active Withdrawn
- 2000-03-28 KR KR1020017012410A patent/KR20010112931A/ko not_active Application Discontinuation
- 2000-03-28 MX MXPA01009875A patent/MXPA01009875A/es unknown
- 2000-03-28 JP JP2000608758A patent/JP2002539835A/ja active Pending
- 2000-03-28 CA CA002365594A patent/CA2365594A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN1353757A (zh) | 2002-06-12 |
| MXPA01009875A (es) | 2002-04-24 |
| AU3843900A (en) | 2000-10-16 |
| BR0010398A (pt) | 2002-12-03 |
| JP2002539835A (ja) | 2002-11-26 |
| EP1165799A1 (en) | 2002-01-02 |
| KR20010112931A (ko) | 2001-12-22 |
| CA2365594A1 (en) | 2000-10-05 |
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