EA200000758A1 - STREPTOMYCES AVERMITILIS GENE CHANGING RELATION B2: B1 - Google Patents

STREPTOMYCES AVERMITILIS GENE CHANGING RELATION B2: B1

Info

Publication number
EA200000758A1
EA200000758A1 EA200000758A EA200000758A EA200000758A1 EA 200000758 A1 EA200000758 A1 EA 200000758A1 EA 200000758 A EA200000758 A EA 200000758A EA 200000758 A EA200000758 A EA 200000758A EA 200000758 A1 EA200000758 A1 EA 200000758A1
Authority
EA
Eurasian Patent Office
Prior art keywords
streptomyces avermitilis
changing relation
classes
ratio
change
Prior art date
Application number
EA200000758A
Other languages
Russian (ru)
Other versions
EA003905B1 (en
Inventor
Ким Джонелл Стацман-Энгуолл
Йошихиро Кэйто
Хеймиш Аластэр Эрвин Макартур
Original Assignee
Пфайзер Продактс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пфайзер Продактс Инк. filed Critical Пфайзер Продактс Инк.
Publication of EA200000758A1 publication Critical patent/EA200000758A1/en
Publication of EA003905B1 publication Critical patent/EA003905B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin

Abstract

Настоящее изобретение относится к полинуклеотидным молекулам, содержащим нуклеотидные последовательности, кодирующие продукт гена aveC, причем эти полинуклеотидные молекулы можно использовать для изменения соотношения или количества авермектинов классов 2:1, продуцируемых в ферментационных культурах S.avermitilis. Кроме того, настоящее изобретение относится к векторам, клеткам-хозяевам и мутантным штаммам S.avermitilis, в которых ген aveC инактивирован или мутирован таким образом, чтобы изменить соотношение или количество продуцируемых авермектинов классов 2:1.Международная заявка была опубликована вместе с отчетом о международном поиске.The present invention relates to polynucleotide molecules containing nucleotide sequences encoding aveC gene product, and these polynucleotide molecules can be used to change the ratio or number of avermectins of classes 2: 1 produced in S. avermitilis fermentation cultures. In addition, the present invention relates to vectors, host cells and mutant strains of S. avermitilis, in which the aveC gene is inactivated or mutated in such a way as to change the ratio or number of classes 2 avermectins produced. 1. The international application search.

EA200000758A 1998-02-13 1999-01-25 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins EA003905B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7463698P 1998-02-13 1998-02-13
PCT/IB1999/000130 WO1999041389A1 (en) 1998-02-13 1999-01-25 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Publications (2)

Publication Number Publication Date
EA200000758A1 true EA200000758A1 (en) 2001-04-23
EA003905B1 EA003905B1 (en) 2003-10-30

Family

ID=22120706

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200000758A EA003905B1 (en) 1998-02-13 1999-01-25 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Country Status (32)

Country Link
EP (1) EP1053335A1 (en)
JP (2) JP2002503473A (en)
KR (3) KR100499214B1 (en)
CN (2) CN1521180A (en)
AP (1) AP9901463A0 (en)
AR (1) AR018085A1 (en)
AU (1) AU752343C (en)
BG (1) BG64911B1 (en)
BR (1) BR9907893A (en)
CA (2) CA2521289A1 (en)
CO (1) CO5070698A1 (en)
DZ (1) DZ2720A1 (en)
EA (1) EA003905B1 (en)
GT (1) GT199900014A (en)
HN (1) HN1999000008A (en)
HR (1) HRP20000539A2 (en)
HU (1) HUP0100784A3 (en)
IL (2) IL137610A0 (en)
IS (1) IS5567A (en)
MA (1) MA24760A1 (en)
NO (1) NO20004044L (en)
NZ (2) NZ518657A (en)
OA (1) OA11449A (en)
PA (1) PA8467601A1 (en)
PE (1) PE20000352A1 (en)
PL (1) PL194270B1 (en)
SK (1) SK11722000A3 (en)
TN (1) TNSN99017A1 (en)
TW (1) TW585910B (en)
WO (1) WO1999041389A1 (en)
YU (1) YU50900A (en)
ZA (1) ZA991138B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111269296A (en) * 2020-03-06 2020-06-12 山东大学 nLsA protein, structural gene thereof and application thereof

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248579B1 (en) * 1998-02-13 2001-06-19 Pfizer Inc Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
EP1053335A1 (en) * 1998-02-13 2000-11-22 Pfizer Products Inc. Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
CA2380872C (en) * 1999-08-12 2007-11-27 Yan Chen Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
US7630836B2 (en) 2001-05-30 2009-12-08 The Kitasato Institute Polynucleotides
ATE426659T1 (en) 2002-02-12 2009-04-15 Pfizer Prod Inc STREPTOMYCES AVERMITILIS GENE REGULATING THE B2:B1 AVERMECTINE RATIO
CN107338210B (en) * 2017-09-05 2021-08-17 天津科技大学 Abamectin streptomycete synthetic medium and preparation method of fermentation liquor thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE434277B (en) * 1976-04-19 1984-07-16 Merck & Co Inc SET TO MAKE NEW ANTIHELMINTICALLY EFFECTIVE ASSOCIATIONS BY CULTIVATING STREPTOMYCS AVERMITILIS
US4429042A (en) * 1978-09-08 1984-01-31 Merck & Co., Inc. Strain of Streptomyces for producing antiparasitic compounds
IN167980B (en) * 1987-01-23 1991-01-19 Pfizer
DE3881147T2 (en) * 1987-10-23 1993-09-02 Pfizer METHOD FOR PRODUCING AGLYCONES OF AVERMECTIN AND CULTURES CONTAINING THEM.
JPH0747171B2 (en) * 1988-09-20 1995-05-24 株式会社東芝 Rolling mill setting method and device
US5252474A (en) * 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
NZ233116A (en) * 1989-03-31 1994-09-27 Merck & Co Inc Cloning genes from steptomyces avermitilis for avermectin biosynthesis
JP2888586B2 (en) * 1990-03-05 1999-05-10 社団法人北里研究所 Microorganism for selective production of specific components of avermectin and its selective production method
FI942725A (en) * 1993-12-16 1995-06-17 Pfizer Genes encoding branched chain alpha-keto acid dehydrogenase complex from Streptomyces avermitilis
EP1053335A1 (en) * 1998-02-13 2000-11-22 Pfizer Products Inc. Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111269296A (en) * 2020-03-06 2020-06-12 山东大学 nLsA protein, structural gene thereof and application thereof
CN111269296B (en) * 2020-03-06 2021-11-05 山东大学 nLsA protein, structural gene thereof and application thereof

Also Published As

Publication number Publication date
DZ2720A1 (en) 2003-09-01
CN1521180A (en) 2004-08-18
AR018085A1 (en) 2001-10-31
KR20040053390A (en) 2004-06-23
AP9901463A0 (en) 1999-03-31
CA2521289A1 (en) 1999-08-19
PL194270B1 (en) 2007-05-31
NZ505676A (en) 2003-02-28
KR20040099389A (en) 2004-11-26
AU1887899A (en) 1999-08-30
KR100499214B1 (en) 2005-07-07
TW585910B (en) 2004-05-01
CA2320031A1 (en) 1999-08-19
GT199900014A (en) 2000-07-25
CN1297485A (en) 2001-05-30
NO20004044D0 (en) 2000-08-11
JP4011036B2 (en) 2007-11-21
MA24760A1 (en) 1999-10-01
HN1999000008A (en) 1999-10-29
PA8467601A1 (en) 2000-09-29
JP2004283174A (en) 2004-10-14
JP2002503473A (en) 2002-02-05
BG64911B1 (en) 2006-08-31
IL183596A0 (en) 2007-09-20
WO1999041389A1 (en) 1999-08-19
IS5567A (en) 2000-07-20
PE20000352A1 (en) 2000-05-16
TNSN99017A1 (en) 2005-11-10
IL137610A0 (en) 2001-07-24
AU752343B2 (en) 2002-09-19
ZA991138B (en) 2000-08-14
BR9907893A (en) 2000-11-14
EA003905B1 (en) 2003-10-30
SK11722000A3 (en) 2001-04-09
OA11449A (en) 2003-12-03
BG104759A (en) 2001-04-30
YU50900A (en) 2004-03-12
HUP0100784A2 (en) 2001-06-28
HRP20000539A2 (en) 2001-04-30
CO5070698A1 (en) 2001-08-28
NO20004044L (en) 2000-10-11
HUP0100784A3 (en) 2004-10-28
AU752343C (en) 2005-11-03
EP1053335A1 (en) 2000-11-22
PL342468A1 (en) 2001-06-04
KR20010052166A (en) 2001-06-25
NZ518657A (en) 2003-10-31

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU