WO2004108906A3 - Bacterial carriers for nucleotide sequences coding for active substances - Google Patents

Bacterial carriers for nucleotide sequences coding for active substances Download PDF

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Publication number
WO2004108906A3
WO2004108906A3 PCT/DE2004/001180 DE2004001180W WO2004108906A3 WO 2004108906 A3 WO2004108906 A3 WO 2004108906A3 DE 2004001180 W DE2004001180 W DE 2004001180W WO 2004108906 A3 WO2004108906 A3 WO 2004108906A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequences
active substances
sequences coding
bacterial
bacterial carriers
Prior art date
Application number
PCT/DE2004/001180
Other languages
German (de)
French (fr)
Other versions
WO2004108906A2 (en
Inventor
Werner Goebel
Sabine Pilgrim
Jochen Strizker
Ivaylo Gentschev
Christoph Schoen
Original Assignee
Medinnova Ges Med Innovationen
Werner Goebel
Sabine Pilgrim
Jochen Strizker
Ivaylo Gentschev
Christoph Schoen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medinnova Ges Med Innovationen, Werner Goebel, Sabine Pilgrim, Jochen Strizker, Ivaylo Gentschev, Christoph Schoen filed Critical Medinnova Ges Med Innovationen
Priority to DE112004001496T priority Critical patent/DE112004001496D2/en
Publication of WO2004108906A2 publication Critical patent/WO2004108906A2/en
Publication of WO2004108906A3 publication Critical patent/WO2004108906A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

The invention relates to a bacterial microorganism containing a foreign nucleic acid sequence that codes for an active substance. A natural nucleic acid sequence of the bacterium, which codes for expression of a bacterial enzyme is deleted or mutated in the chromosomal DNA microorganism provided that a translation product obtained therefrom is not functional. The invention also relates to cells that are infected with such microorganisms, methods for producing such organisms, and the use thereof, especially for producing pharmaceutical compositions.
PCT/DE2004/001180 2003-06-06 2004-06-07 Bacterial carriers for nucleotide sequences coding for active substances WO2004108906A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112004001496T DE112004001496D2 (en) 2003-06-06 2004-06-07 Bacterial carriers for nucleotide sequences encoding drugs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10326189A DE10326189A1 (en) 2003-06-06 2003-06-06 Bacterial carriers for nucleotide sequences encoding drugs
DE10326189.3 2003-06-06

Publications (2)

Publication Number Publication Date
WO2004108906A2 WO2004108906A2 (en) 2004-12-16
WO2004108906A3 true WO2004108906A3 (en) 2005-02-10

Family

ID=33494938

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2004/001180 WO2004108906A2 (en) 2003-06-06 2004-06-07 Bacterial carriers for nucleotide sequences coding for active substances

Country Status (2)

Country Link
DE (2) DE10326189A1 (en)
WO (1) WO2004108906A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048026A1 (en) * 1997-04-18 1998-10-29 Gesellschaft für Biotechnologische Forschung mbH Attenuated salmonella strain used as a vehicle for oral immunization
US5877159A (en) * 1995-05-03 1999-03-02 University Of Maryland At Baltimore Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same
WO1999034007A1 (en) * 1997-12-29 1999-07-08 Schering Aktiengesellschaft Delivery of polypeptide-encoding plasmid dna into the cytosol of macrophages by attenuated suicide bacteria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877159A (en) * 1995-05-03 1999-03-02 University Of Maryland At Baltimore Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same
WO1998048026A1 (en) * 1997-04-18 1998-10-29 Gesellschaft für Biotechnologische Forschung mbH Attenuated salmonella strain used as a vehicle for oral immunization
WO1999034007A1 (en) * 1997-12-29 1999-07-08 Schering Aktiengesellschaft Delivery of polypeptide-encoding plasmid dna into the cytosol of macrophages by attenuated suicide bacteria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THOMPSON R J ET AL: "Pathogenicity and Immunogenicity of a Listeria monocytogenes Strain That Requires D-Alanine for Growth", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 66, no. 8, 1998, pages 3552 - 3561, XP002170925, ISSN: 0019-9567 *

Also Published As

Publication number Publication date
WO2004108906A2 (en) 2004-12-16
DE10326189A1 (en) 2005-01-05
DE112004001496D2 (en) 2006-04-20

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