EA022877B1 - Полинуклеотид гена стрелкования b сахарной свеклы и его применение - Google Patents
Полинуклеотид гена стрелкования b сахарной свеклы и его применение Download PDFInfo
- Publication number
- EA022877B1 EA022877B1 EA200901525A EA200901525A EA022877B1 EA 022877 B1 EA022877 B1 EA 022877B1 EA 200901525 A EA200901525 A EA 200901525A EA 200901525 A EA200901525 A EA 200901525A EA 022877 B1 EA022877 B1 EA 022877B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- sequence
- sugar beet
- nucleotide sequence
- polynucleotide
- gene
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 294
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 184
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 184
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 184
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 title claims abstract description 173
- 235000021536 Sugar beet Nutrition 0.000 title claims abstract description 125
- 241000196324 Embryophyta Species 0.000 claims abstract description 233
- 238000000034 method Methods 0.000 claims abstract description 109
- 238000011161 development Methods 0.000 claims abstract description 100
- 102000054766 genetic haplotypes Human genes 0.000 claims abstract description 23
- 238000013507 mapping Methods 0.000 claims abstract description 17
- 101150076489 B gene Proteins 0.000 claims abstract description 6
- 239000002773 nucleotide Substances 0.000 claims description 266
- 125000003729 nucleotide group Chemical group 0.000 claims description 262
- 239000012634 fragment Substances 0.000 claims description 145
- 239000000523 sample Substances 0.000 claims description 91
- 108700028369 Alleles Proteins 0.000 claims description 84
- 230000003321 amplification Effects 0.000 claims description 76
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 76
- 239000003550 marker Substances 0.000 claims description 60
- 230000002441 reversible effect Effects 0.000 claims description 50
- 238000004458 analytical method Methods 0.000 claims description 33
- 102000054765 polymorphisms of proteins Human genes 0.000 claims description 31
- 230000000295 complement effect Effects 0.000 claims description 24
- 239000011159 matrix material Substances 0.000 claims description 21
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 13
- 241000335053 Beta vulgaris Species 0.000 claims description 13
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007850 fluorescent dye Substances 0.000 claims description 12
- 101001024425 Mus musculus Ig gamma-2A chain C region secreted form Proteins 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 10
- 239000000975 dye Substances 0.000 claims description 8
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 8
- 229940104302 cytosine Drugs 0.000 claims description 6
- 239000003147 molecular marker Substances 0.000 claims description 6
- 238000012163 sequencing technique Methods 0.000 claims description 5
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 4
- 229940113082 thymine Drugs 0.000 claims description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 3
- 229930024421 Adenine Natural products 0.000 claims description 3
- 229960000643 adenine Drugs 0.000 claims description 3
- 238000011109 contamination Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims 4
- 239000003068 molecular probe Substances 0.000 claims 1
- 238000005070 sampling Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000002955 isolation Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 239000013615 primer Substances 0.000 description 199
- 108020004414 DNA Proteins 0.000 description 120
- 102000053602 DNA Human genes 0.000 description 118
- 230000014509 gene expression Effects 0.000 description 106
- 230000018109 developmental process Effects 0.000 description 75
- 210000004027 cell Anatomy 0.000 description 60
- 238000003752 polymerase chain reaction Methods 0.000 description 47
- 230000002068 genetic effect Effects 0.000 description 45
- 210000001519 tissue Anatomy 0.000 description 43
- 230000009261 transgenic effect Effects 0.000 description 42
- 238000009396 hybridization Methods 0.000 description 41
- 102000004169 proteins and genes Human genes 0.000 description 36
- 230000001105 regulatory effect Effects 0.000 description 36
- 150000007523 nucleic acids Chemical group 0.000 description 31
- 238000013518 transcription Methods 0.000 description 30
- 230000035897 transcription Effects 0.000 description 30
- 108091028043 Nucleic acid sequence Proteins 0.000 description 29
- 108091026890 Coding region Proteins 0.000 description 28
- 102000039446 nucleic acids Human genes 0.000 description 27
- 108020004707 nucleic acids Proteins 0.000 description 27
- 229920002477 rna polymer Polymers 0.000 description 24
- 230000009466 transformation Effects 0.000 description 24
- 238000012217 deletion Methods 0.000 description 23
- 230000037430 deletion Effects 0.000 description 23
- 125000003275 alpha amino acid group Chemical group 0.000 description 21
- 238000013459 approach Methods 0.000 description 21
- 240000008042 Zea mays Species 0.000 description 20
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 20
- 108090000765 processed proteins & peptides Proteins 0.000 description 20
- 239000013598 vector Substances 0.000 description 20
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 20
- 238000003780 insertion Methods 0.000 description 19
- 230000037431 insertion Effects 0.000 description 19
- 229920001184 polypeptide Polymers 0.000 description 18
- 102000004196 processed proteins & peptides Human genes 0.000 description 18
- 230000000694 effects Effects 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 14
- 235000005822 corn Nutrition 0.000 description 14
- 230000035772 mutation Effects 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 13
- 241000894007 species Species 0.000 description 13
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Chemical class Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 12
- 239000002299 complementary DNA Substances 0.000 description 12
- 238000012216 screening Methods 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 108700019146 Transgenes Proteins 0.000 description 11
- 230000000692 anti-sense effect Effects 0.000 description 11
- 230000002018 overexpression Effects 0.000 description 11
- 230000001629 suppression Effects 0.000 description 11
- 230000004019 vernalization response Effects 0.000 description 11
- 238000010835 comparative analysis Methods 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 10
- 230000001131 transforming effect Effects 0.000 description 10
- 239000013604 expression vector Substances 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 235000021537 Beetroot Nutrition 0.000 description 8
- 230000001939 inductive effect Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 108090000994 Catalytic RNA Proteins 0.000 description 7
- 102000053642 Catalytic RNA Human genes 0.000 description 7
- 235000010469 Glycine max Nutrition 0.000 description 7
- 108091092195 Intron Proteins 0.000 description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 description 7
- 244000061456 Solanum tuberosum Species 0.000 description 7
- 229910052796 boron Inorganic materials 0.000 description 7
- 238000004925 denaturation Methods 0.000 description 7
- 230000036425 denaturation Effects 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 235000013305 food Nutrition 0.000 description 7
- 230000001404 mediated effect Effects 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 108091092562 ribozyme Proteins 0.000 description 7
- 238000006467 substitution reaction Methods 0.000 description 7
- 239000011701 zinc Substances 0.000 description 7
- 241000701489 Cauliflower mosaic virus Species 0.000 description 6
- 108091092878 Microsatellite Proteins 0.000 description 6
- 241000700605 Viruses Species 0.000 description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 6
- 239000000356 contaminant Substances 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 235000009973 maize Nutrition 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 229910052725 zinc Inorganic materials 0.000 description 6
- 241000724328 Alfalfa mosaic virus Species 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 5
- 108700024394 Exon Proteins 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 244000061176 Nicotiana tabacum Species 0.000 description 5
- 101710089395 Oleosin Proteins 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 241000723873 Tobacco mosaic virus Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 238000003776 cleavage reaction Methods 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000007834 ligase chain reaction Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 238000004153 renaturation Methods 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 230000007017 scission Effects 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 108020005544 Antisense RNA Proteins 0.000 description 4
- 235000002566 Capsicum Nutrition 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- RRTCJVBFMMTZSS-UHFFFAOYSA-N SSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSS RRTCJVBFMMTZSS-UHFFFAOYSA-N 0.000 description 4
- 240000003768 Solanum lycopersicum Species 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 239000003184 complementary RNA Substances 0.000 description 4
- 238000004520 electroporation Methods 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000030279 gene silencing Effects 0.000 description 4
- 230000001976 improved effect Effects 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 235000012015 potatoes Nutrition 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 101150011812 AADAC gene Proteins 0.000 description 3
- 108091023037 Aptamer Proteins 0.000 description 3
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 3
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 3
- 206010021929 Infertility male Diseases 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 3
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 240000003889 Piper guineense Species 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- ZBQPJUMVWHDCRQ-UHFFFAOYSA-N SSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSS ZBQPJUMVWHDCRQ-UHFFFAOYSA-N 0.000 description 3
- 235000007238 Secale cereale Nutrition 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 230000001364 causal effect Effects 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000002060 circadian Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000004009 herbicide Substances 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 230000008121 plant development Effects 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 240000007087 Apium graveolens Species 0.000 description 2
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 2
- 235000010591 Appio Nutrition 0.000 description 2
- 101100435119 Arabidopsis thaliana APRR1 gene Proteins 0.000 description 2
- 235000000832 Ayote Nutrition 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 240000001980 Cucurbita pepo Species 0.000 description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 102100034349 Integrase Human genes 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 101100058903 Mus musculus Ca13 gene Proteins 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- 240000004713 Pisum sativum Species 0.000 description 2
- 108700001094 Plant Genes Proteins 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- 101100481792 Schizosaccharomyces pombe (strain 972 / ATCC 24843) toc1 gene Proteins 0.000 description 2
- 244000082988 Secale cereale Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 108700026226 TATA Box Proteins 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 108700040099 Xylose isomerases Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 230000027288 circadian rhythm Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000005714 functional activity Effects 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002743 insertional mutagenesis Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- -1 light Substances 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000031787 nutrient reservoir activity Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 239000002987 primer (paints) Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 235000015136 pumpkin Nutrition 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 101150065190 term gene Proteins 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 description 1
- 101150101112 7 gene Proteins 0.000 description 1
- 101150018711 AASS gene Proteins 0.000 description 1
- 101150026843 AP2 gene Proteins 0.000 description 1
- 244000144619 Abrus precatorius Species 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 240000002234 Allium sativum Species 0.000 description 1
- 235000013479 Amaranthus retroflexus Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 101100059544 Arabidopsis thaliana CDC5 gene Proteins 0.000 description 1
- 101000982406 Arachis hypogaea Oleosin Ara h 15.0101 Proteins 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 101710116822 Atrochrysone carboxylic acid synthase Proteins 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 102000000131 Beta tubulin Human genes 0.000 description 1
- 244000233287 Beta vulgaris ssp maritima Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 244000188595 Brassica sinapistrum Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 101100109216 Caenorhabditis elegans apy-1 gene Proteins 0.000 description 1
- 101100324465 Caenorhabditis elegans arr-1 gene Proteins 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 244000298479 Cichorium intybus Species 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 235000017788 Cydonia oblonga Nutrition 0.000 description 1
- 241000274965 Cyrestis thyodamas Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 101500011079 Diploptera punctata Allatostatin-11 Proteins 0.000 description 1
- 241000710188 Encephalomyocarditis virus Species 0.000 description 1
- 102100038132 Endogenous retrovirus group K member 6 Pro protein Human genes 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 101710091045 Envelope protein Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 235000012601 Euterpe oleracea Nutrition 0.000 description 1
- 244000207620 Euterpe oleracea Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 244000060234 Gmelina philippensis Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000583811 Homo sapiens Mitotic spindle assembly checkpoint protein MAD2B Proteins 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010021928 Infertility female Diseases 0.000 description 1
- 108010061833 Integrases Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 1
- 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 101150115300 MAC1 gene Proteins 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 244000081841 Malus domestica Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 102100030955 Mitotic spindle assembly checkpoint protein MAD2B Human genes 0.000 description 1
- 101100518501 Mus musculus Spp1 gene Proteins 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 235000009421 Myristica fragrans Nutrition 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 240000004370 Pastinaca sativa Species 0.000 description 1
- 235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
- 241000792395 Patellifolia procumbens Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101710188315 Protein X Proteins 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
- 101000932966 Pseudomonas aeruginosa CD-NTase-associated protein 8 Proteins 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
- 101150023114 RNA1 gene Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 235000017848 Rubus fruticosus Nutrition 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- OTSMHWLYYJVJDL-UHFFFAOYSA-N SSSSSSSSS Chemical compound SSSSSSSSS OTSMHWLYYJVJDL-UHFFFAOYSA-N 0.000 description 1
- SPUUKOIOZNVGHK-UHFFFAOYSA-N SSSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSSS SPUUKOIOZNVGHK-UHFFFAOYSA-N 0.000 description 1
- OMGVLZMIEYQVLH-UHFFFAOYSA-N SSSSSSSSSSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSSSSSSSSSS OMGVLZMIEYQVLH-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 235000008406 SarachaNachtschatten Nutrition 0.000 description 1
- 101100412105 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rec7 gene Proteins 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- 235000004790 Solanum aculeatissimum Nutrition 0.000 description 1
- 235000008424 Solanum demissum Nutrition 0.000 description 1
- 235000018253 Solanum ferox Nutrition 0.000 description 1
- 235000000208 Solanum incanum Nutrition 0.000 description 1
- 235000013131 Solanum macrocarpon Nutrition 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000009869 Solanum phureja Nutrition 0.000 description 1
- 240000002307 Solanum ptychanthum Species 0.000 description 1
- 235000000341 Solanum ptychanthum Nutrition 0.000 description 1
- 235000017622 Solanum xanthocarpum Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 108010043934 Sucrose synthase Proteins 0.000 description 1
- JDZPLYBLBIKFHJ-UHFFFAOYSA-N Sulfamoyldapsone Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=C1S(N)(=O)=O JDZPLYBLBIKFHJ-UHFFFAOYSA-N 0.000 description 1
- 101710101744 Sulfur-rich protein Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 244000078534 Vaccinium myrtillus Species 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- GBXZONVFWYCRPT-KVTDHHQDSA-N [(2s,3s,4r,5r)-3,4,5,6-tetrahydroxy-1-oxohexan-2-yl] dihydrogen phosphate Chemical class OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](C=O)OP(O)(O)=O GBXZONVFWYCRPT-KVTDHHQDSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 235000003650 acai Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000729 antidote Substances 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 208000036815 beta tubulin Diseases 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000021029 blackberry Nutrition 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- ZINJLDJMHCUBIP-UHFFFAOYSA-N ethametsulfuron-methyl Chemical compound CCOC1=NC(NC)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)C(=O)OC)=N1 ZINJLDJMHCUBIP-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000004883 flower formation Effects 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000001115 mace Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000000302 molecular modelling Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- IHKWXDCSAKJQKM-SRQGCSHVSA-N n-[(1s,6s,7r,8r,8ar)-1,7,8-trihydroxy-1,2,3,5,6,7,8,8a-octahydroindolizin-6-yl]acetamide Chemical compound O[C@H]1[C@H](O)[C@@H](NC(=O)C)CN2CC[C@H](O)[C@@H]21 IHKWXDCSAKJQKM-SRQGCSHVSA-N 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 230000001020 rhythmical effect Effects 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 229960001860 salicylate Drugs 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000012106 screening analysis Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 102000023888 sequence-specific DNA binding proteins Human genes 0.000 description 1
- 108091008420 sequence-specific DNA binding proteins Proteins 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000004350 spin decoupling difference spectroscopy Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000000352 storage cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07108777 | 2007-05-23 | ||
| PCT/EP2008/056390 WO2008142167A2 (en) | 2007-05-23 | 2008-05-23 | Polynucleotide markers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200901525A1 EA200901525A1 (ru) | 2010-10-29 |
| EA022877B1 true EA022877B1 (ru) | 2016-03-31 |
Family
ID=39529427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200901525A EA022877B1 (ru) | 2007-05-23 | 2008-05-23 | Полинуклеотид гена стрелкования b сахарной свеклы и его применение |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20100209919A1 (enExample) |
| EP (3) | EP2152885B1 (enExample) |
| JP (2) | JP2010527598A (enExample) |
| CN (2) | CN101772575B (enExample) |
| BR (1) | BRPI0812387A2 (enExample) |
| CA (2) | CA2687760C (enExample) |
| DK (1) | DK2152885T3 (enExample) |
| EA (1) | EA022877B1 (enExample) |
| MA (1) | MA31495B1 (enExample) |
| PL (1) | PL2152885T3 (enExample) |
| UA (2) | UA100855C2 (enExample) |
| WO (1) | WO2008142167A2 (enExample) |
| ZA (1) | ZA200907678B (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009141446A1 (en) * | 2008-05-23 | 2009-11-26 | Syngenta Participations Ag | Transgenic sugar beet plants |
| EP2152885B1 (en) * | 2007-05-23 | 2014-06-18 | Syngenta Participations AG | Polynucleotide markers |
| EA028355B1 (ru) * | 2008-05-23 | 2017-11-30 | Зингента Партисипейшнс Аг | Трансгенные растения сахарной свеклы |
| EP2754078A4 (en) * | 2011-04-14 | 2015-12-02 | Complete Genomics Inc | PROCESSING AND ANALYSIS OF COMPLEX NUCLEIC ACID SEQUENCE DATA |
| WO2013103367A1 (en) * | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
| NZ615098A (en) * | 2012-09-13 | 2015-05-29 | Seminis Vegetable Seeds Inc | Genetic markers for myb28 |
| KR101962669B1 (ko) * | 2016-06-08 | 2019-03-27 | 동아대학교 산학협력단 | 푸자리움 푸지쿠로이 종복합체 구분을 위한 프라이머 선별방법 및 프라이머 세트 |
| KR101962636B1 (ko) * | 2016-06-08 | 2019-03-27 | 동아대학교 산학협력단 | 실린드로카폰 데스트럭탄스균을 선별하기 위한 프라이머 선별방법 및 프라이머 세트 |
| CN107267660B (zh) * | 2017-08-21 | 2020-10-09 | 黑龙江大学 | 检测甜菜雄蕊育性的ssr分子标记及其应用 |
| US11149298B2 (en) | 2018-06-13 | 2021-10-19 | International Business Machines Corporation | Detection of nucleic acid sequences using deterministic lateral displacement arrays |
| US11180796B2 (en) | 2018-06-13 | 2021-11-23 | International Business Machines Corporation | Detection of mutations regarding one or more deoxyribonucleic acid sequences using deterministic lateral displacement arrays |
| CN109825604B (zh) * | 2019-04-03 | 2022-12-13 | 中国农业科学院郑州果树研究所 | 与桃抗蚜性状紧密连锁的分子标记，用于检测桃抗蚜性状的引物、试剂盒、方法及其应用 |
| CN110305981A (zh) * | 2019-08-05 | 2019-10-08 | 辽宁省果树科学研究所 | 一种杏果实硬度关键基因的分子标记pg1524及其检测方法和应用 |
| CN110527740B (zh) * | 2019-09-11 | 2022-03-22 | 东北农业大学 | 一种与印度南瓜强雌基因紧密连锁的分子标记、引物及应用 |
| CN111518916B (zh) * | 2020-02-28 | 2022-07-15 | 南京农业大学 | 猪13号染色体与二花脸猪产活仔数显著相关的snp标记及其检测方法与应用 |
| CN116121437B (zh) * | 2022-11-07 | 2023-10-13 | 中国热带农业科学院南亚热带作物研究所 | 一种杧果品种的snp标记组合及杧果育种中的应用 |
| CN117625635A (zh) * | 2023-10-31 | 2024-03-01 | 长江大学 | 甘蓝型油菜BnPPD基因、突变体及其应用 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| EP0138341B1 (en) | 1983-09-03 | 1988-10-26 | Bass Public Limited Company | Beer and other beverages and their manufacture |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| EP0846771A1 (en) | 1987-05-20 | 1998-06-10 | Novartis AG | Zea mays plants and transgenic zea mays plants regenerated from protoplasts or protoplast-derived cells |
| US5350689A (en) | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
| NZ225044A (en) | 1987-06-19 | 1990-01-29 | Plant Cell Res Inst | Bertholletia excelsa dna molecule; sulphur rich storage protein |
| DE58909753D1 (de) | 1988-03-08 | 1997-01-23 | Ciba Geigy Ag | Regeneration von fertilen Gramineen-Pflanzen aus der Unterfamilie Pooideae ausgehend von Protoplasten |
| WO1990001069A1 (en) | 1988-07-20 | 1990-02-08 | Segev Diagnostics, Inc. | Process for amplifying and detecting nucleic acid sequences |
| US5501967A (en) | 1989-07-26 | 1996-03-26 | Mogen International, N.V./Rijksuniversiteit Te Leiden | Process for the site-directed integration of DNA into the genome of plants |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| FR2673642B1 (fr) | 1991-03-05 | 1994-08-12 | Rhone Poulenc Agrochimie | Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate. |
| HU218034B (hu) | 1992-01-09 | 2000-05-28 | Novartis Ag. | Új növényi promoter |
| US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| DE69534629D1 (de) | 1994-01-18 | 2005-12-29 | Scripps Research Inst | Derivate von zinkfingerproteinen und methoden |
| USRE39229E1 (en) | 1994-08-20 | 2006-08-08 | Gendaq Limited | Binding proteins for recognition of DNA |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| CA2325344C (en) | 1998-04-08 | 2017-12-05 | Commonwealth Scientific And Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
| AR020078A1 (es) | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| US20100293669A2 (en) * | 1999-05-06 | 2010-11-18 | Jingdong Liu | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US20070234438A1 (en) * | 2000-05-08 | 2007-10-04 | Abad Mark S | Nucleic acid molecules and other molecules associated with plants |
| US20040181830A1 (en) * | 2001-05-07 | 2004-09-16 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| WO2002014523A2 (en) | 2000-08-11 | 2002-02-21 | Syngenta Participations Ag | Methods for stable transformation of plants |
| BRPI0411002A (pt) * | 2003-06-06 | 2006-05-23 | Arborgen Llc | polinucleotìdios isolados, fator de transcrição de planta, seqüências de nucleotìdios isolados, construções de dna, células de planta, plantas transgênicas, polpa de madeira, combinações de detecção de expressão de um ou mais polinucleotìdios, microconjunto e kit e métodos de produção de planta transgênica, de rastreamento de promotor, de correlação da expressão de polinucleotìdio em duas amostras diferentes e da posse de um fenótipo de planta com o nìvel de expressão de polinucleotìdio na planta de um ou mais polinucleotìdios e da detecção de um ou mais polinucleotìdios numa amostra |
| US20060150283A1 (en) * | 2004-02-13 | 2006-07-06 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| BRPI0614050A2 (pt) * | 2005-06-30 | 2011-03-09 | Syngenta Participations Ag | métodos para peneiramento de polimorfismos de hibridização especìficos de gene (gshps) e seus usos no mapeamento genético e desenvolvimento de marcador |
| CN101466839B (zh) * | 2006-04-21 | 2012-07-25 | 先正达参股股份有限公司 | 控制甜菜抽苔的转基因植株和方法 |
| EP2152885B1 (en) * | 2007-05-23 | 2014-06-18 | Syngenta Participations AG | Polynucleotide markers |
-
2008
- 2008-05-23 EP EP08759990.8A patent/EP2152885B1/en not_active Not-in-force
- 2008-05-23 CN CN200880100228.0A patent/CN101772575B/zh not_active Expired - Fee Related
- 2008-05-23 JP JP2010508869A patent/JP2010527598A/ja active Pending
- 2008-05-23 WO PCT/EP2008/056390 patent/WO2008142167A2/en not_active Ceased
- 2008-05-23 BR BRPI0812387-0A2A patent/BRPI0812387A2/pt not_active Application Discontinuation
- 2008-05-23 UA UAA200913022A patent/UA100855C2/ru unknown
- 2008-05-23 EP EP11161309A patent/EP2390335A3/en not_active Withdrawn
- 2008-05-23 PL PL08759990T patent/PL2152885T3/pl unknown
- 2008-05-23 DK DK08759990.8T patent/DK2152885T3/da active
- 2008-05-23 US US12/601,386 patent/US20100209919A1/en not_active Abandoned
- 2008-05-23 EA EA200901525A patent/EA022877B1/ru not_active IP Right Cessation
- 2008-05-23 EP EP08156812A patent/EP1995320A3/en not_active Withdrawn
- 2008-05-23 CA CA2687760A patent/CA2687760C/en active Active
-
2009
- 2009-05-25 CA CA2724419A patent/CA2724419C/en active Active
- 2009-05-25 US US12/994,452 patent/US8796506B2/en not_active Expired - Fee Related
- 2009-05-25 JP JP2011510002A patent/JP2011520461A/ja active Pending
- 2009-05-25 UA UAA201015429A patent/UA105489C2/uk unknown
- 2009-05-25 CN CN200980121061.0A patent/CN102057045B/zh not_active Expired - Fee Related
- 2009-11-02 ZA ZA200907678A patent/ZA200907678B/xx unknown
- 2009-11-20 MA MA32367A patent/MA31495B1/fr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2390335A3 (en) | 2012-03-21 |
| ZA200907678B (en) | 2010-07-28 |
| CA2687760A1 (en) | 2008-11-27 |
| WO2008142167A3 (en) | 2009-03-12 |
| DK2152885T3 (da) | 2014-08-18 |
| US20110138492A1 (en) | 2011-06-09 |
| EP1995320A2 (en) | 2008-11-26 |
| PL2152885T3 (pl) | 2014-11-28 |
| JP2010527598A (ja) | 2010-08-19 |
| EP1995320A3 (en) | 2009-01-07 |
| EP2390335A2 (en) | 2011-11-30 |
| UA100855C2 (en) | 2013-02-11 |
| EA200901525A1 (ru) | 2010-10-29 |
| CA2724419A1 (en) | 2009-11-26 |
| JP2011520461A (ja) | 2011-07-21 |
| EP2152885B1 (en) | 2014-06-18 |
| US8796506B2 (en) | 2014-08-05 |
| CN101772575A (zh) | 2010-07-07 |
| CN101772575B (zh) | 2015-03-11 |
| MA31495B1 (fr) | 2010-07-01 |
| US20100209919A1 (en) | 2010-08-19 |
| BRPI0812387A2 (pt) | 2014-11-04 |
| EP2152885A2 (en) | 2010-02-17 |
| CA2687760C (en) | 2017-10-31 |
| WO2008142167A2 (en) | 2008-11-27 |
| CA2724419C (en) | 2018-11-13 |
| CN102057045B (zh) | 2018-06-05 |
| UA105489C2 (uk) | 2014-05-26 |
| CN102057045A (zh) | 2011-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA022877B1 (ru) | Полинуклеотид гена стрелкования b сахарной свеклы и его применение | |
| Cai et al. | CRISPR/Cas9‐mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean | |
| Miura et al. | OsSPL14 promotes panicle branching and higher grain productivity in rice | |
| US12234467B2 (en) | Diplospory gene | |
| EP3018217A2 (en) | Maize cytoplasmic male sterility (cms) c-type restorer rf4 gene, molecular markers and their use | |
| CN106086064B (zh) | 控制水稻株高、提高抗倒伏能力、增加有效分蘖数和产量的基因及其应用 | |
| WO2019038417A1 (en) | METHODS FOR INCREASING GRAIN YIELD | |
| EA024958B1 (ru) | Конструирование устойчивости к выходу в стрелку у сахарной свеклы с помощью трансгенной экспрессии гомолога гена ft свеклы, контролирующего время цветения | |
| CN112521471B (zh) | 一个控制玉米籽粒含水量的基因和分子标记及其应用 | |
| US20220154202A1 (en) | Gene Regulating Seed Weight in Improving Seed Yield in Soybean | |
| US20200255846A1 (en) | Methods for increasing grain yield | |
| AU2021404992A1 (en) | New native clubroot resistance in rapeseed brassica napus | |
| CN104520312B (zh) | 种子活力的调节 | |
| BR112021006948A2 (pt) | Novos alelos cenh3 de trigo | |
| Ju et al. | StOFP20 regulates tuber shape and interacts with TONNEAU1 recruiting motif proteins in potato | |
| CN118684748A (zh) | 一种微肽及其应用 | |
| JP2016523510A (ja) | 変異型ｄａ１対立遺伝子を含んでなるアブラナ属植物 | |
| US20210348180A1 (en) | Terminator sequence for gene expression in plants | |
| CN118531044A (zh) | 控制玉米籽粒脱水的启动子、乙烯信号通路基因及其应用 | |
| JP2023526035A (ja) | 標的突然変異生成によって変異体植物を得るための方法 | |
| US20250197953A1 (en) | Methods of determining sensitivity to photoperiod in cannabis | |
| EA028355B1 (ru) | Трансгенные растения сахарной свеклы | |
| Fulneček et al. | The origin of exon 3 skipping of paternal GLOBOSA pre-mRNA in some Nicotiana tabacum lines correlates with a point mutation of the very last nucleotide of the exon | |
| JP2025525862A (ja) | フラボノイド依存性稔性 | |
| EP4601457A1 (en) | Virus and insect resistance and markers in barley |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ KZ KG TJ TM |
|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY MD RU |