EA022877B1 - Полинуклеотид гена стрелкования b сахарной свеклы и его применение - Google Patents
Полинуклеотид гена стрелкования b сахарной свеклы и его применение Download PDFInfo
- Publication number
- EA022877B1 EA022877B1 EA200901525A EA200901525A EA022877B1 EA 022877 B1 EA022877 B1 EA 022877B1 EA 200901525 A EA200901525 A EA 200901525A EA 200901525 A EA200901525 A EA 200901525A EA 022877 B1 EA022877 B1 EA 022877B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- sequence
- sugar beet
- nucleotide sequence
- polynucleotide
- gene
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 294
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 184
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 184
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 184
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 title claims abstract description 173
- 235000021536 Sugar beet Nutrition 0.000 title claims abstract description 125
- 241000196324 Embryophyta Species 0.000 claims abstract description 233
- 238000000034 method Methods 0.000 claims abstract description 109
- 238000011161 development Methods 0.000 claims abstract description 100
- 102000054766 genetic haplotypes Human genes 0.000 claims abstract description 23
- 238000013507 mapping Methods 0.000 claims abstract description 17
- 101150076489 B gene Proteins 0.000 claims abstract description 6
- 239000002773 nucleotide Substances 0.000 claims description 266
- 125000003729 nucleotide group Chemical group 0.000 claims description 262
- 239000012634 fragment Substances 0.000 claims description 145
- 239000000523 sample Substances 0.000 claims description 91
- 108700028369 Alleles Proteins 0.000 claims description 84
- 230000003321 amplification Effects 0.000 claims description 76
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 76
- 239000003550 marker Substances 0.000 claims description 60
- 230000002441 reversible effect Effects 0.000 claims description 50
- 238000004458 analytical method Methods 0.000 claims description 33
- 102000054765 polymorphisms of proteins Human genes 0.000 claims description 31
- 230000000295 complement effect Effects 0.000 claims description 24
- 239000011159 matrix material Substances 0.000 claims description 21
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 13
- 241000335053 Beta vulgaris Species 0.000 claims description 13
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007850 fluorescent dye Substances 0.000 claims description 12
- 101001024425 Mus musculus Ig gamma-2A chain C region secreted form Proteins 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 10
- 239000000975 dye Substances 0.000 claims description 8
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 8
- 229940104302 cytosine Drugs 0.000 claims description 6
- 239000003147 molecular marker Substances 0.000 claims description 6
- 238000012163 sequencing technique Methods 0.000 claims description 5
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 4
- 229940113082 thymine Drugs 0.000 claims description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 3
- 229930024421 Adenine Natural products 0.000 claims description 3
- 229960000643 adenine Drugs 0.000 claims description 3
- 238000011109 contamination Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims 4
- 239000003068 molecular probe Substances 0.000 claims 1
- 238000005070 sampling Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000002955 isolation Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 239000013615 primer Substances 0.000 description 199
- 108020004414 DNA Proteins 0.000 description 120
- 102000053602 DNA Human genes 0.000 description 118
- 230000014509 gene expression Effects 0.000 description 106
- 230000018109 developmental process Effects 0.000 description 75
- 210000004027 cell Anatomy 0.000 description 60
- 238000003752 polymerase chain reaction Methods 0.000 description 47
- 230000002068 genetic effect Effects 0.000 description 45
- 210000001519 tissue Anatomy 0.000 description 43
- 230000009261 transgenic effect Effects 0.000 description 42
- 238000009396 hybridization Methods 0.000 description 41
- 102000004169 proteins and genes Human genes 0.000 description 36
- 230000001105 regulatory effect Effects 0.000 description 36
- 150000007523 nucleic acids Chemical group 0.000 description 31
- 238000013518 transcription Methods 0.000 description 30
- 230000035897 transcription Effects 0.000 description 30
- 108091028043 Nucleic acid sequence Proteins 0.000 description 29
- 108091026890 Coding region Proteins 0.000 description 28
- 102000039446 nucleic acids Human genes 0.000 description 27
- 108020004707 nucleic acids Proteins 0.000 description 27
- 229920002477 rna polymer Polymers 0.000 description 24
- 230000009466 transformation Effects 0.000 description 24
- 238000012217 deletion Methods 0.000 description 23
- 230000037430 deletion Effects 0.000 description 23
- 125000003275 alpha amino acid group Chemical group 0.000 description 21
- 238000013459 approach Methods 0.000 description 21
- 240000008042 Zea mays Species 0.000 description 20
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 20
- 108090000765 processed proteins & peptides Proteins 0.000 description 20
- 239000013598 vector Substances 0.000 description 20
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 20
- 238000003780 insertion Methods 0.000 description 19
- 230000037431 insertion Effects 0.000 description 19
- 229920001184 polypeptide Polymers 0.000 description 18
- 102000004196 processed proteins & peptides Human genes 0.000 description 18
- 230000000694 effects Effects 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 14
- 235000005822 corn Nutrition 0.000 description 14
- 230000035772 mutation Effects 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 13
- 241000894007 species Species 0.000 description 13
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Chemical class Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 12
- 239000002299 complementary DNA Substances 0.000 description 12
- 238000012216 screening Methods 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 108700019146 Transgenes Proteins 0.000 description 11
- 230000000692 anti-sense effect Effects 0.000 description 11
- 230000002018 overexpression Effects 0.000 description 11
- 230000001629 suppression Effects 0.000 description 11
- 230000004019 vernalization response Effects 0.000 description 11
- 238000010835 comparative analysis Methods 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 10
- 230000001131 transforming effect Effects 0.000 description 10
- 239000013604 expression vector Substances 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 235000021537 Beetroot Nutrition 0.000 description 8
- 230000001939 inductive effect Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 108090000994 Catalytic RNA Proteins 0.000 description 7
- 102000053642 Catalytic RNA Human genes 0.000 description 7
- 235000010469 Glycine max Nutrition 0.000 description 7
- 108091092195 Intron Proteins 0.000 description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 description 7
- 244000061456 Solanum tuberosum Species 0.000 description 7
- 229910052796 boron Inorganic materials 0.000 description 7
- 238000004925 denaturation Methods 0.000 description 7
- 230000036425 denaturation Effects 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 235000013305 food Nutrition 0.000 description 7
- 230000001404 mediated effect Effects 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 108091092562 ribozyme Proteins 0.000 description 7
- 238000006467 substitution reaction Methods 0.000 description 7
- 239000011701 zinc Substances 0.000 description 7
- 241000701489 Cauliflower mosaic virus Species 0.000 description 6
- 108091092878 Microsatellite Proteins 0.000 description 6
- 241000700605 Viruses Species 0.000 description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 6
- 239000000356 contaminant Substances 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 235000009973 maize Nutrition 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 229910052725 zinc Inorganic materials 0.000 description 6
- 241000724328 Alfalfa mosaic virus Species 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 5
- 108700024394 Exon Proteins 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 244000061176 Nicotiana tabacum Species 0.000 description 5
- 101710089395 Oleosin Proteins 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 241000723873 Tobacco mosaic virus Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 238000003776 cleavage reaction Methods 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000007834 ligase chain reaction Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 238000004153 renaturation Methods 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 230000007017 scission Effects 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 108020005544 Antisense RNA Proteins 0.000 description 4
- 235000002566 Capsicum Nutrition 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- RRTCJVBFMMTZSS-UHFFFAOYSA-N SSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSS RRTCJVBFMMTZSS-UHFFFAOYSA-N 0.000 description 4
- 240000003768 Solanum lycopersicum Species 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 239000003184 complementary RNA Substances 0.000 description 4
- 238000004520 electroporation Methods 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000030279 gene silencing Effects 0.000 description 4
- 230000001976 improved effect Effects 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 235000012015 potatoes Nutrition 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 101150011812 AADAC gene Proteins 0.000 description 3
- 108091023037 Aptamer Proteins 0.000 description 3
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 3
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 3
- 206010021929 Infertility male Diseases 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 3
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 240000003889 Piper guineense Species 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- ZBQPJUMVWHDCRQ-UHFFFAOYSA-N SSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSS ZBQPJUMVWHDCRQ-UHFFFAOYSA-N 0.000 description 3
- 235000007238 Secale cereale Nutrition 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 230000001364 causal effect Effects 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000002060 circadian Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000004009 herbicide Substances 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 230000008121 plant development Effects 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 240000007087 Apium graveolens Species 0.000 description 2
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 2
- 235000010591 Appio Nutrition 0.000 description 2
- 101100435119 Arabidopsis thaliana APRR1 gene Proteins 0.000 description 2
- 235000000832 Ayote Nutrition 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 240000001980 Cucurbita pepo Species 0.000 description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 102100034349 Integrase Human genes 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 101100058903 Mus musculus Ca13 gene Proteins 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- 240000004713 Pisum sativum Species 0.000 description 2
- 108700001094 Plant Genes Proteins 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- 101100481792 Schizosaccharomyces pombe (strain 972 / ATCC 24843) toc1 gene Proteins 0.000 description 2
- 244000082988 Secale cereale Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 108700026226 TATA Box Proteins 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 108700040099 Xylose isomerases Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 230000027288 circadian rhythm Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000005714 functional activity Effects 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002743 insertional mutagenesis Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- -1 light Substances 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000031787 nutrient reservoir activity Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 239000002987 primer (paints) Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 235000015136 pumpkin Nutrition 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 101150065190 term gene Proteins 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 description 1
- 101150101112 7 gene Proteins 0.000 description 1
- 101150018711 AASS gene Proteins 0.000 description 1
- 101150026843 AP2 gene Proteins 0.000 description 1
- 244000144619 Abrus precatorius Species 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 240000002234 Allium sativum Species 0.000 description 1
- 235000013479 Amaranthus retroflexus Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 101100059544 Arabidopsis thaliana CDC5 gene Proteins 0.000 description 1
- 101000982406 Arachis hypogaea Oleosin Ara h 15.0101 Proteins 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 101710116822 Atrochrysone carboxylic acid synthase Proteins 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 102000000131 Beta tubulin Human genes 0.000 description 1
- 244000233287 Beta vulgaris ssp maritima Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 244000188595 Brassica sinapistrum Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 101100109216 Caenorhabditis elegans apy-1 gene Proteins 0.000 description 1
- 101100324465 Caenorhabditis elegans arr-1 gene Proteins 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 244000298479 Cichorium intybus Species 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 235000017788 Cydonia oblonga Nutrition 0.000 description 1
- 241000274965 Cyrestis thyodamas Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 101500011079 Diploptera punctata Allatostatin-11 Proteins 0.000 description 1
- 241000710188 Encephalomyocarditis virus Species 0.000 description 1
- 102100038132 Endogenous retrovirus group K member 6 Pro protein Human genes 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 101710091045 Envelope protein Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 235000012601 Euterpe oleracea Nutrition 0.000 description 1
- 244000207620 Euterpe oleracea Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 244000060234 Gmelina philippensis Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000583811 Homo sapiens Mitotic spindle assembly checkpoint protein MAD2B Proteins 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010021928 Infertility female Diseases 0.000 description 1
- 108010061833 Integrases Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 1
- 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 101150115300 MAC1 gene Proteins 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 244000081841 Malus domestica Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 102100030955 Mitotic spindle assembly checkpoint protein MAD2B Human genes 0.000 description 1
- 101100518501 Mus musculus Spp1 gene Proteins 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 235000009421 Myristica fragrans Nutrition 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 240000004370 Pastinaca sativa Species 0.000 description 1
- 235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
- 241000792395 Patellifolia procumbens Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101710188315 Protein X Proteins 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
- 101000932966 Pseudomonas aeruginosa CD-NTase-associated protein 8 Proteins 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
- 101150023114 RNA1 gene Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 235000017848 Rubus fruticosus Nutrition 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- OTSMHWLYYJVJDL-UHFFFAOYSA-N SSSSSSSSS Chemical compound SSSSSSSSS OTSMHWLYYJVJDL-UHFFFAOYSA-N 0.000 description 1
- SPUUKOIOZNVGHK-UHFFFAOYSA-N SSSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSSS SPUUKOIOZNVGHK-UHFFFAOYSA-N 0.000 description 1
- OMGVLZMIEYQVLH-UHFFFAOYSA-N SSSSSSSSSSSSSSSSSSSSSS Chemical compound SSSSSSSSSSSSSSSSSSSSSS OMGVLZMIEYQVLH-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 235000008406 SarachaNachtschatten Nutrition 0.000 description 1
- 101100412105 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rec7 gene Proteins 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- 235000004790 Solanum aculeatissimum Nutrition 0.000 description 1
- 235000008424 Solanum demissum Nutrition 0.000 description 1
- 235000018253 Solanum ferox Nutrition 0.000 description 1
- 235000000208 Solanum incanum Nutrition 0.000 description 1
- 235000013131 Solanum macrocarpon Nutrition 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000009869 Solanum phureja Nutrition 0.000 description 1
- 240000002307 Solanum ptychanthum Species 0.000 description 1
- 235000000341 Solanum ptychanthum Nutrition 0.000 description 1
- 235000017622 Solanum xanthocarpum Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 108010043934 Sucrose synthase Proteins 0.000 description 1
- JDZPLYBLBIKFHJ-UHFFFAOYSA-N Sulfamoyldapsone Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=C1S(N)(=O)=O JDZPLYBLBIKFHJ-UHFFFAOYSA-N 0.000 description 1
- 101710101744 Sulfur-rich protein Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 244000078534 Vaccinium myrtillus Species 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- GBXZONVFWYCRPT-KVTDHHQDSA-N [(2s,3s,4r,5r)-3,4,5,6-tetrahydroxy-1-oxohexan-2-yl] dihydrogen phosphate Chemical class OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](C=O)OP(O)(O)=O GBXZONVFWYCRPT-KVTDHHQDSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 235000003650 acai Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000729 antidote Substances 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 208000036815 beta tubulin Diseases 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000021029 blackberry Nutrition 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- ZINJLDJMHCUBIP-UHFFFAOYSA-N ethametsulfuron-methyl Chemical compound CCOC1=NC(NC)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)C(=O)OC)=N1 ZINJLDJMHCUBIP-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000004883 flower formation Effects 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000001115 mace Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000000302 molecular modelling Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- IHKWXDCSAKJQKM-SRQGCSHVSA-N n-[(1s,6s,7r,8r,8ar)-1,7,8-trihydroxy-1,2,3,5,6,7,8,8a-octahydroindolizin-6-yl]acetamide Chemical compound O[C@H]1[C@H](O)[C@@H](NC(=O)C)CN2CC[C@H](O)[C@@H]21 IHKWXDCSAKJQKM-SRQGCSHVSA-N 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 230000001020 rhythmical effect Effects 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 229960001860 salicylate Drugs 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000012106 screening analysis Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 102000023888 sequence-specific DNA binding proteins Human genes 0.000 description 1
- 108091008420 sequence-specific DNA binding proteins Proteins 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000004350 spin decoupling difference spectroscopy Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000000352 storage cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07108777 | 2007-05-23 | ||
| PCT/EP2008/056390 WO2008142167A2 (en) | 2007-05-23 | 2008-05-23 | Polynucleotide markers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200901525A1 EA200901525A1 (ru) | 2010-10-29 |
| EA022877B1 true EA022877B1 (ru) | 2016-03-31 |
Family
ID=39529427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200901525A EA022877B1 (ru) | 2007-05-23 | 2008-05-23 | Полинуклеотид гена стрелкования b сахарной свеклы и его применение |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20100209919A1 (enExample) |
| EP (3) | EP1995320A3 (enExample) |
| JP (2) | JP2010527598A (enExample) |
| CN (2) | CN101772575B (enExample) |
| BR (1) | BRPI0812387A2 (enExample) |
| CA (2) | CA2687760C (enExample) |
| DK (1) | DK2152885T3 (enExample) |
| EA (1) | EA022877B1 (enExample) |
| MA (1) | MA31495B1 (enExample) |
| PL (1) | PL2152885T3 (enExample) |
| UA (2) | UA100855C2 (enExample) |
| WO (1) | WO2008142167A2 (enExample) |
| ZA (1) | ZA200907678B (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008142167A2 (en) * | 2007-05-23 | 2008-11-27 | Syngenta Participations Ag | Polynucleotide markers |
| WO2009141446A1 (en) * | 2008-05-23 | 2009-11-26 | Syngenta Participations Ag | Transgenic sugar beet plants |
| PL2291525T3 (pl) * | 2008-05-23 | 2015-08-31 | Syngenta Participations Ag | Transgeniczne rośliny buraka cukrowego |
| CA2833165A1 (en) * | 2011-04-14 | 2012-10-18 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| WO2013103367A1 (en) * | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
| NZ706930A (en) * | 2012-09-13 | 2017-01-27 | Seminis Vegetable Seeds Inc | Genetic markers for myb28 |
| KR101962636B1 (ko) * | 2016-06-08 | 2019-03-27 | 동아대학교 산학협력단 | 실린드로카폰 데스트럭탄스균을 선별하기 위한 프라이머 선별방법 및 프라이머 세트 |
| KR101962669B1 (ko) * | 2016-06-08 | 2019-03-27 | 동아대학교 산학협력단 | 푸자리움 푸지쿠로이 종복합체 구분을 위한 프라이머 선별방법 및 프라이머 세트 |
| CN107267660B (zh) * | 2017-08-21 | 2020-10-09 | 黑龙江大学 | 检测甜菜雄蕊育性的ssr分子标记及其应用 |
| US11149298B2 (en) | 2018-06-13 | 2021-10-19 | International Business Machines Corporation | Detection of nucleic acid sequences using deterministic lateral displacement arrays |
| US11180796B2 (en) | 2018-06-13 | 2021-11-23 | International Business Machines Corporation | Detection of mutations regarding one or more deoxyribonucleic acid sequences using deterministic lateral displacement arrays |
| CN109825604B (zh) * | 2019-04-03 | 2022-12-13 | 中国农业科学院郑州果树研究所 | 与桃抗蚜性状紧密连锁的分子标记，用于检测桃抗蚜性状的引物、试剂盒、方法及其应用 |
| CN110305981A (zh) * | 2019-08-05 | 2019-10-08 | 辽宁省果树科学研究所 | 一种杏果实硬度关键基因的分子标记pg1524及其检测方法和应用 |
| CN110527740B (zh) * | 2019-09-11 | 2022-03-22 | 东北农业大学 | 一种与印度南瓜强雌基因紧密连锁的分子标记、引物及应用 |
| CN111518916B (zh) * | 2020-02-28 | 2022-07-15 | 南京农业大学 | 猪13号染色体与二花脸猪产活仔数显著相关的snp标记及其检测方法与应用 |
| CN116121437B (zh) * | 2022-11-07 | 2023-10-13 | 中国热带农业科学院南亚热带作物研究所 | 一种杧果品种的snp标记组合及杧果育种中的应用 |
| CN117625635A (zh) * | 2023-10-31 | 2024-03-01 | 长江大学 | 甘蓝型油菜BnPPD基因、突变体及其应用 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| DE3474837D1 (en) | 1983-09-03 | 1988-12-01 | Bass Plc | Beer and other beverages and their manufacture |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| ES2074999T3 (es) | 1987-05-20 | 1995-10-01 | Ciba Geigy Ag | Plantas de zea mays y plantas de zea mays transgenicas regeneradas de protoplastos o celulas derivadas de protoplastos. |
| US5350689A (en) | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
| NZ225044A (en) | 1987-06-19 | 1990-01-29 | Plant Cell Res Inst | Bertholletia excelsa dna molecule; sulphur rich storage protein |
| ES2097746T3 (es) | 1988-03-08 | 1997-04-16 | Ciba Geigy Ag | Regeneracion de plantas gramineas de la subfamilia pooideae partiendo de protoplastos. |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5501967A (en) | 1989-07-26 | 1996-03-26 | Mogen International, N.V./Rijksuniversiteit Te Leiden | Process for the site-directed integration of DNA into the genome of plants |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| FR2673642B1 (fr) | 1991-03-05 | 1994-08-12 | Rhone Poulenc Agrochimie | Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate. |
| HU218034B (hu) | 1992-01-09 | 2000-05-28 | Novartis Ag. | Új növényi promoter |
| DE69534629D1 (de) | 1994-01-18 | 2005-12-29 | Scripps Research Inst | Derivate von zinkfingerproteinen und methoden |
| US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| USRE39229E1 (en) | 1994-08-20 | 2006-08-08 | Gendaq Limited | Binding proteins for recognition of DNA |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| AU760041B2 (en) | 1998-04-08 | 2003-05-08 | Commonwealth Scientific And Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
| AR020078A1 (es) | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US20100293669A2 (en) * | 1999-05-06 | 2010-11-18 | Jingdong Liu | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| US20070234438A1 (en) * | 2000-05-08 | 2007-10-04 | Abad Mark S | Nucleic acid molecules and other molecules associated with plants |
| US20040181830A1 (en) * | 2001-05-07 | 2004-09-16 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| EP2290090A1 (en) | 2000-08-11 | 2011-03-02 | Syngenta Participations AG | Methods for stable transformation of plants |
| NZ583631A (en) * | 2003-06-06 | 2011-04-29 | Arborgen Inc | MYB tanscription factor for regulating plant gene expression |
| US20060150283A1 (en) * | 2004-02-13 | 2006-07-06 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| CN101213312A (zh) * | 2005-06-30 | 2008-07-02 | 先正达参股股份有限公司 | 用于筛选基因特异杂交多态性（gshp）的方法及其在遗传作图和标记开发中的用途 |
| CA2648499A1 (en) * | 2006-04-21 | 2007-11-01 | Syngenta Participations Ag | Transgenic plants and methods for controlling bolting in sugar beet |
| WO2008142167A2 (en) * | 2007-05-23 | 2008-11-27 | Syngenta Participations Ag | Polynucleotide markers |
-
2008
- 2008-05-23 WO PCT/EP2008/056390 patent/WO2008142167A2/en not_active Ceased
- 2008-05-23 EA EA200901525A patent/EA022877B1/ru not_active IP Right Cessation
- 2008-05-23 CN CN200880100228.0A patent/CN101772575B/zh not_active Expired - Fee Related
- 2008-05-23 DK DK08759990.8T patent/DK2152885T3/da active
- 2008-05-23 CA CA2687760A patent/CA2687760C/en active Active
- 2008-05-23 UA UAA200913022A patent/UA100855C2/ru unknown
- 2008-05-23 BR BRPI0812387-0A2A patent/BRPI0812387A2/pt not_active Application Discontinuation
- 2008-05-23 EP EP08156812A patent/EP1995320A3/en not_active Withdrawn
- 2008-05-23 EP EP11161309A patent/EP2390335A3/en not_active Withdrawn
- 2008-05-23 EP EP08759990.8A patent/EP2152885B1/en not_active Not-in-force
- 2008-05-23 PL PL08759990T patent/PL2152885T3/pl unknown
- 2008-05-23 US US12/601,386 patent/US20100209919A1/en not_active Abandoned
- 2008-05-23 JP JP2010508869A patent/JP2010527598A/ja active Pending
-
2009
- 2009-05-25 UA UAA201015429A patent/UA105489C2/uk unknown
- 2009-05-25 JP JP2011510002A patent/JP2011520461A/ja active Pending
- 2009-05-25 CN CN200980121061.0A patent/CN102057045B/zh not_active Expired - Fee Related
- 2009-05-25 US US12/994,452 patent/US8796506B2/en not_active Expired - Fee Related
- 2009-05-25 CA CA2724419A patent/CA2724419C/en active Active
- 2009-11-02 ZA ZA200907678A patent/ZA200907678B/xx unknown
- 2009-11-20 MA MA32367A patent/MA31495B1/fr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EA200901525A1 (ru) | 2010-10-29 |
| EP2152885A2 (en) | 2010-02-17 |
| WO2008142167A3 (en) | 2009-03-12 |
| CN102057045A (zh) | 2011-05-11 |
| BRPI0812387A2 (pt) | 2014-11-04 |
| CN102057045B (zh) | 2018-06-05 |
| UA100855C2 (en) | 2013-02-11 |
| CA2687760A1 (en) | 2008-11-27 |
| EP1995320A2 (en) | 2008-11-26 |
| ZA200907678B (en) | 2010-07-28 |
| US8796506B2 (en) | 2014-08-05 |
| JP2011520461A (ja) | 2011-07-21 |
| EP2152885B1 (en) | 2014-06-18 |
| CA2724419A1 (en) | 2009-11-26 |
| CA2687760C (en) | 2017-10-31 |
| CN101772575B (zh) | 2015-03-11 |
| CA2724419C (en) | 2018-11-13 |
| EP1995320A3 (en) | 2009-01-07 |
| US20100209919A1 (en) | 2010-08-19 |
| EP2390335A3 (en) | 2012-03-21 |
| MA31495B1 (fr) | 2010-07-01 |
| WO2008142167A2 (en) | 2008-11-27 |
| CN101772575A (zh) | 2010-07-07 |
| EP2390335A2 (en) | 2011-11-30 |
| JP2010527598A (ja) | 2010-08-19 |
| US20110138492A1 (en) | 2011-06-09 |
| UA105489C2 (uk) | 2014-05-26 |
| PL2152885T3 (pl) | 2014-11-28 |
| DK2152885T3 (da) | 2014-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA022877B1 (ru) | Полинуклеотид гена стрелкования b сахарной свеклы и его применение | |
| Cai et al. | CRISPR/Cas9‐mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean | |
| Miura et al. | OsSPL14 promotes panicle branching and higher grain productivity in rice | |
| US12234467B2 (en) | Diplospory gene | |
| EP3018217A2 (en) | Maize cytoplasmic male sterility (cms) c-type restorer rf4 gene, molecular markers and their use | |
| CN106086064B (zh) | 控制水稻株高、提高抗倒伏能力、增加有效分蘖数和产量的基因及其应用 | |
| WO2019038417A1 (en) | METHODS FOR INCREASING GRAIN YIELD | |
| EA024958B1 (ru) | Конструирование устойчивости к выходу в стрелку у сахарной свеклы с помощью трансгенной экспрессии гомолога гена ft свеклы, контролирующего время цветения | |
| US20220154202A1 (en) | Gene Regulating Seed Weight in Improving Seed Yield in Soybean | |
| CN112521471B (zh) | 一个控制玉米籽粒含水量的基因和分子标记及其应用 | |
| US20200255846A1 (en) | Methods for increasing grain yield | |
| AU2021404992A9 (en) | New native clubroot resistance in rapeseed brassica napus | |
| CN104520312B (zh) | 种子活力的调节 | |
| Ju et al. | StOFP20 regulates tuber shape and interacts with TONNEAU1 Recruiting Motif proteins in potato | |
| BR112021006948A2 (pt) | Novos alelos cenh3 de trigo | |
| JP2016523510A (ja) | 変異型ｄａ１対立遺伝子を含んでなるアブラナ属植物 | |
| US20210348180A1 (en) | Terminator sequence for gene expression in plants | |
| CN118684748A (zh) | 一种微肽及其应用 | |
| JP2023526035A (ja) | 標的突然変異生成によって変異体植物を得るための方法 | |
| US20250197953A1 (en) | Methods of determining sensitivity to photoperiod in cannabis | |
| EA028355B1 (ru) | Трансгенные растения сахарной свеклы | |
| Fulneček et al. | The origin of exon 3 skipping of paternal GLOBOSA pre-mRNA in some Nicotiana tabacum lines correlates with a point mutation of the very last nucleotide of the exon | |
| JP2025525862A (ja) | フラボノイド依存性稔性 | |
| AU2023359496A1 (en) | Virus and insect resistance and markers in barley | |
| EA049404B1 (ru) | Повышенная устойчивость кукурузы к гельминтоспориозной пятнистости листьев кукурузы посредством локуса количественных признаков на хромосоме 4 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ KZ KG TJ TM |
|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY MD RU |