DK3152331T3 - Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner - Google Patents
Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner Download PDFInfo
- Publication number
- DK3152331T3 DK3152331T3 DK15802743T DK15802743T DK3152331T3 DK 3152331 T3 DK3152331 T3 DK 3152331T3 DK 15802743 T DK15802743 T DK 15802743T DK 15802743 T DK15802743 T DK 15802743T DK 3152331 T3 DK3152331 T3 DK 3152331T3
- Authority
- DK
- Denmark
- Prior art keywords
- ligase
- polymerase
- copy
- identifying
- expression
- Prior art date
Links
- 230000007067 DNA methylation Effects 0.000 title 1
- 102000003960 Ligases Human genes 0.000 title 1
- 108090000364 Ligases Proteins 0.000 title 1
- 101710163270 Nuclease Proteins 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462009047P | 2014-06-06 | 2014-06-06 | |
| US201562136093P | 2015-03-20 | 2015-03-20 | |
| PCT/US2015/034724 WO2015188192A2 (en) | 2014-06-06 | 2015-06-08 | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3152331T3 true DK3152331T3 (da) | 2019-11-04 |
Family
ID=54767613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15802743T DK3152331T3 (da) | 2014-06-06 | 2015-06-08 | Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10407722B2 (enExample) |
| EP (4) | EP3889271B1 (enExample) |
| JP (3) | JP7008407B2 (enExample) |
| CN (2) | CN113046413A (enExample) |
| AU (2) | AU2015269103B2 (enExample) |
| CA (2) | CA3213538A1 (enExample) |
| DK (1) | DK3152331T3 (enExample) |
| ES (3) | ES2935285T3 (enExample) |
| IL (1) | IL249126B (enExample) |
| PT (3) | PT3889271T (enExample) |
| SG (2) | SG11201610134PA (enExample) |
| WO (1) | WO2015188192A2 (enExample) |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2707744T3 (es) | 2013-12-11 | 2019-04-04 | Accuragen Holdings Ltd | Métodos para detectar variantes de secuencia raras |
| US11859246B2 (en) * | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| EP3889271B1 (en) * | 2014-06-06 | 2022-11-23 | Cornell University | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
| US11085073B2 (en) * | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
| WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
| WO2017089520A2 (en) | 2015-11-25 | 2017-06-01 | Genia Technologies, Inc. | Purification of polymerase complexes |
| CN108699505A (zh) * | 2015-12-03 | 2018-10-23 | 安可济控股有限公司 | 用于形成连接产物的方法和组合物 |
| US20210214781A1 (en) * | 2016-02-14 | 2021-07-15 | Abhijit Ajit Patel | Measurement of nucleic acid |
| JP7143221B2 (ja) * | 2016-04-07 | 2022-09-28 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | ５－ヒドロキシメチル化無細胞系ｄｎａをシーケンシングすることによる非侵襲性診断 |
| CA3025956A1 (en) * | 2016-07-01 | 2018-01-04 | Natera, Inc. | Compositions and methods for detection of nucleic acid mutations |
| CN109844137B (zh) * | 2016-10-31 | 2022-04-26 | 豪夫迈·罗氏有限公司 | 用于鉴定嵌合产物的条形码化环状文库构建 |
| EP3559269B1 (en) * | 2016-12-20 | 2020-09-16 | F. Hoffmann-La Roche AG | Single stranded circular dna libraries for circular consensus sequencing |
| CN109234388B (zh) * | 2017-07-04 | 2021-09-14 | 深圳华大生命科学研究院 | 用于dna高甲基化区域富集的试剂、富集方法及应用 |
| EP3682025A1 (en) * | 2017-09-14 | 2020-07-22 | H. Hoffnabb-La Roche Ag | Novel method for generating circular single-stranded dna libraries |
| EP3682027B1 (en) * | 2017-09-15 | 2025-02-26 | F. Hoffmann-La Roche AG | Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| WO2019090147A1 (en) * | 2017-11-03 | 2019-05-09 | Guardant Health, Inc. | Correcting for deamination-induced sequence errors |
| WO2019024341A1 (zh) * | 2017-11-27 | 2019-02-07 | 深圳华大生命科学研究院 | 一种体液游离dna的文库构建方法及其应用 |
| CN111936634B (zh) | 2017-12-11 | 2025-05-09 | Umc乌德勒支控股有限公司 | 用于制备用于测序的核酸分子的方法 |
| WO2019157339A1 (en) * | 2018-02-09 | 2019-08-15 | Children's Medical Center Corporation | Compositions and methods for identifying a single-nucleotide variant |
| JP7013960B2 (ja) * | 2018-03-13 | 2022-02-01 | 株式会社リコー | 核酸検出方法及び核酸検出装置、並びに、核酸検出キット |
| US11203782B2 (en) | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
| CA3095292A1 (en) | 2018-04-02 | 2019-10-10 | Progenity, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| EP3775198A4 (en) * | 2018-04-02 | 2022-01-05 | Grail, Inc. | METHYLATION MARKERS AND TARGETED METHYLATION PROBE PANELS |
| CN110456034B (zh) * | 2018-05-07 | 2022-07-22 | 上海市第十人民医院 | 一种循环肿瘤细胞的检测方法 |
| US12049665B2 (en) | 2018-06-12 | 2024-07-30 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
| US10876148B2 (en) * | 2018-11-14 | 2020-12-29 | Element Biosciences, Inc. | De novo surface preparation and uses thereof |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| WO2020102766A2 (en) | 2018-11-15 | 2020-05-22 | Element Biosciences, Inc. | Methods for generating circular nucleic acid molecules |
| US20220090183A1 (en) * | 2019-02-25 | 2022-03-24 | Elegen Corporation | Methods of using microfluidic positional encoding devices |
| JP7344973B2 (ja) * | 2019-03-01 | 2023-09-14 | マーシー バイオアナリティクス， インコーポレイテッド | 標的実体を検出するためのシステム、組成物、及び方法 |
| WO2020180813A1 (en) * | 2019-03-06 | 2020-09-10 | Qiagen Sciences, Llc | Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing |
| WO2020206170A1 (en) | 2019-04-02 | 2020-10-08 | Progenity, Inc. | Methods, systems, and compositions for counting nucleic acid molecules |
| BR112022001539A2 (pt) * | 2019-05-13 | 2022-03-22 | Rapid Genomics Llc | Captura e análise de regiões genômicas alvo |
| CN112029841B (zh) * | 2019-06-03 | 2024-02-09 | 香港中文大学 | 定量端粒长度和基因组基序的方法 |
| CN112175939A (zh) * | 2019-07-03 | 2021-01-05 | 华大青兰生物科技(无锡)有限公司 | 一种基于同源序列的核酸拼接方法及其应用 |
| US12091669B2 (en) * | 2019-08-22 | 2024-09-17 | National University Of Singapore | Method for the generation of dumbbell-shaped DNA vectors |
| WO2021036995A1 (en) * | 2019-08-23 | 2021-03-04 | Nanjing University | Direct microrna sequencing using enzyme assisted sequencing |
| CN114641581A (zh) * | 2019-10-28 | 2022-06-17 | 深圳华大智造科技股份有限公司 | Dna接头寡核苷酸 |
| CN118834950A (zh) | 2019-11-06 | 2024-10-25 | 斯坦福大学托管董事会 | 用于分析核酸分子的方法和系统 |
| CN111154820B (zh) * | 2020-01-13 | 2021-12-21 | 上海韦翰斯生物医药科技有限公司 | 一种降低核酸扩增复制滑移率的方法 |
| BR112021026795A2 (pt) * | 2020-02-26 | 2022-02-15 | Illumina Cambridge Ltd | Kits para genotipagem |
| EP4114966B1 (en) * | 2020-03-03 | 2025-02-26 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing double stranded nucleic acids |
| WO2021185320A1 (en) * | 2020-03-18 | 2021-09-23 | Mgi Tech Co., Ltd. | Restoring phase in massively parallel sequencing |
| US12371743B2 (en) * | 2022-03-04 | 2025-07-29 | Element Biosciences, Inc. | Double-stranded splint adaptors and methods of use |
| US12359193B2 (en) | 2022-03-04 | 2025-07-15 | Element Biosciences, Inc. | Single-stranded splint strands and methods of use |
| US12469162B2 (en) | 2020-08-31 | 2025-11-11 | Element Biosciences, Inc. | Primary analysis in next generation sequencing |
| WO2022101162A1 (en) * | 2020-11-13 | 2022-05-19 | Miltenyi Biotec B.V. & Co. KG | Paired end sequential sequencing based on rolling circle amplification |
| EP4001432A1 (en) * | 2020-11-13 | 2022-05-25 | Miltenyi Biotec B.V. & Co. KG | Algorithmic method for efficient indexing of genetic sequences using associative arrays |
| CN112631562B (zh) * | 2020-12-01 | 2022-08-23 | 上海欧易生物医学科技有限公司 | 基于python的二代测序样本混样方法、应用、设备、计算机可读存储介质 |
| ES2960777T3 (es) * | 2021-03-18 | 2024-03-06 | Genomill Health Oy | Métodos para la cuantificación paralela precisa de ácidos nucleicos en muestras diluidas o no purificadas |
| US11783912B2 (en) | 2021-05-05 | 2023-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for analyzing nucleic acid molecules |
| CN113552191B (zh) * | 2021-07-28 | 2023-11-21 | 江苏师范大学 | 基于多层dna放大回路检测甲基化dna的比例型电化学传感器的构建方法 |
| CN117858958A (zh) * | 2021-08-16 | 2024-04-09 | 10X基因组学有限公司 | 包含分割条形码区的探针和使用方法 |
| WO2023122104A2 (en) * | 2021-12-21 | 2023-06-29 | Ultima Genomics, Inc. | Systems and methods for library preparation adapters |
| IL293202A (en) * | 2022-05-22 | 2023-12-01 | Nucleix Ltd | Useful combinations of restriction enzymes |
| WO2024011145A1 (en) * | 2022-07-05 | 2024-01-11 | Element Biosciences, Inc. | Pcr-free library preparation using double-stranded splint adaptors and methods of use |
| US11970736B2 (en) * | 2022-08-31 | 2024-04-30 | Genomill Health Oy | Methods for accurate parallel detection and quantification of nucleic acids |
| US20240068010A1 (en) * | 2022-08-31 | 2024-02-29 | Genomill Health Oy | Highly sensitive methods for accurate parallel quantification of variant nucleic acids |
| CA3267226A1 (en) | 2022-09-12 | 2024-03-21 | Element Biosciences, Inc. | DOUBLE STRAND TO STRAND SPLINT ADAPTERS, UNIVERSAL LONG SPLINTS AND METHODS OF USE |
| CN115491414A (zh) * | 2022-09-21 | 2022-12-20 | 安徽中医药大学第一附属医院(安徽省中医院) | 类风湿关节炎全转录组m6A甲基化修饰差异表达的综合分析方法 |
| WO2024084440A1 (en) * | 2022-10-19 | 2024-04-25 | Biofidelity Ltd | Nucleic acid enrichment and detection |
| WO2025076733A1 (zh) * | 2023-10-11 | 2025-04-17 | 深圳华大智造科技股份有限公司 | 一种快速进行核酸文库构建的方法 |
| WO2025076596A1 (en) * | 2023-10-12 | 2025-04-17 | Ethyr Holdings Pty Ltd | Target recycled hybridisation chain reaction |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0912761A4 (en) * | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION |
| US5736373A (en) | 1996-09-23 | 1998-04-07 | Becton, Dickinson And Company | Thermostable DNA polymerase from Bacillus pallidus |
| US20040086892A1 (en) | 2002-11-06 | 2004-05-06 | Crothers Donald M. | Universal tag assay |
| WO2006030455A1 (en) | 2004-09-17 | 2006-03-23 | Prokaria Ehf. | Dna polymerases having strand displacement activity |
| US20080311626A1 (en) | 2004-09-17 | 2008-12-18 | Arkea Hf | Dna Polymerases Having Strand Displacement Activity |
| WO2006076650A2 (en) | 2005-01-12 | 2006-07-20 | Applera Corporation | Compositions, methods, and kits for selective amplification of nucleic acids |
| AU2006242387B2 (en) * | 2005-04-29 | 2011-01-06 | Synthetic Genomics, Inc. | Amplification and cloning of single DNA molecules using rolling circle amplification |
| US20120083018A1 (en) | 2005-10-06 | 2012-04-05 | Schoenfeld Thomas W | Thermostable dna polymerases and methods of use |
| ATE537251T1 (de) | 2005-10-06 | 2011-12-15 | Lucigen Corp | Temperaturstabile virale polymerasen und verwendungsverfahren |
| WO2007076461A1 (en) | 2005-12-22 | 2007-07-05 | Ge Healthcare Bio-Sciences Corp. | Thermostable dna polymerase from thermus scotoductus |
| WO2007092538A2 (en) * | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| DE102006020885A1 (de) | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
| JP4962899B2 (ja) | 2006-08-31 | 2012-06-27 | 東洋製罐株式会社 | 環状１本鎖ｄｎａの製造方法 |
| US20080096258A1 (en) * | 2006-10-24 | 2008-04-24 | Christian Korfhage | Rolling circle amplification of circular genomes |
| US8962253B2 (en) * | 2009-04-13 | 2015-02-24 | Somagenics Inc. | Methods and compositions for detection of small RNAs |
| WO2010126614A2 (en) * | 2009-04-30 | 2010-11-04 | Good Start Genetics, Inc. | Methods and compositions for evaluating genetic markers |
| US9249192B2 (en) | 2009-08-21 | 2016-02-02 | Virginia Tech Intellectual Properties, Inc. | Infectious genomic DNA clone and serological profile of Torque teno sus virus 1 and 2 |
| AU2010317019B2 (en) * | 2009-11-06 | 2014-10-30 | The Chinese University Of Hong Kong | Size-based genomic analysis |
| WO2012003374A2 (en) * | 2010-07-02 | 2012-01-05 | The Board Of Trustees Of The Leland Stanford Junior University | Targeted sequencing library preparation by genomic dna circularization |
| GB201018714D0 (en) | 2010-11-05 | 2010-12-22 | Oxitec Ltd | Strand displacement activity of modified polymerases and uses thereof |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| EP2718866B1 (en) * | 2011-06-07 | 2020-09-09 | Koninklijke Philips N.V. | Providing nucleotide sequence data |
| WO2013012440A2 (en) | 2011-07-21 | 2013-01-24 | Cornell University | Methods and devices for dna sequencing and molecular diagnostics |
| AU2012304328B2 (en) * | 2011-09-09 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| US20150126376A1 (en) | 2012-06-14 | 2015-05-07 | Fred Hutchinson Cancer Research Center | Compositions and methods for sensitive mutation detection in nucleic acid molecules |
| US20140066317A1 (en) | 2012-09-04 | 2014-03-06 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| WO2014047561A1 (en) | 2012-09-21 | 2014-03-27 | The Broad Institute Inc. | Compositions and methods for labeling of agents |
| CN105026577B (zh) * | 2013-03-05 | 2020-01-03 | 安捷伦科技有限公司 | 通过序列捕获检测基因组重排 |
| CN113832216A (zh) * | 2013-03-15 | 2021-12-24 | 莱尔·J·阿诺德 | 使用发卡寡核苷酸扩增核酸的方法 |
| EP2981609B1 (en) | 2013-04-05 | 2020-04-29 | Bioron GmbH | Novel dna-polymerases |
| ES2707744T3 (es) * | 2013-12-11 | 2019-04-04 | Accuragen Holdings Ltd | Métodos para detectar variantes de secuencia raras |
| EP3889271B1 (en) * | 2014-06-06 | 2022-11-23 | Cornell University | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
-
2015
- 2015-06-08 EP EP21157460.3A patent/EP3889271B1/en active Active
- 2015-06-08 ES ES21157460T patent/ES2935285T3/es active Active
- 2015-06-08 SG SG11201610134PA patent/SG11201610134PA/en unknown
- 2015-06-08 EP EP22201393.0A patent/EP4170044A1/en active Pending
- 2015-06-08 CA CA3213538A patent/CA3213538A1/en active Pending
- 2015-06-08 EP EP15802743.3A patent/EP3152331B1/en active Active
- 2015-06-08 AU AU2015269103A patent/AU2015269103B2/en not_active Ceased
- 2015-06-08 PT PT211574603T patent/PT3889271T/pt unknown
- 2015-06-08 JP JP2016571229A patent/JP7008407B2/ja active Active
- 2015-06-08 ES ES15802743T patent/ES2748457T3/es active Active
- 2015-06-08 PT PT158027433T patent/PT3152331T/pt unknown
- 2015-06-08 ES ES19178828T patent/ES2864531T3/es active Active
- 2015-06-08 CN CN202110270831.0A patent/CN113046413A/zh active Pending
- 2015-06-08 EP EP19178828.0A patent/EP3567122B1/en active Active
- 2015-06-08 SG SG10201810862YA patent/SG10201810862YA/en unknown
- 2015-06-08 DK DK15802743T patent/DK3152331T3/da active
- 2015-06-08 PT PT191788280T patent/PT3567122T/pt unknown
- 2015-06-08 CA CA2950295A patent/CA2950295C/en active Active
- 2015-06-08 CN CN201580042765.4A patent/CN106661631B/zh active Active
- 2015-06-08 US US15/316,778 patent/US10407722B2/en active Active
- 2015-06-08 WO PCT/US2015/034724 patent/WO2015188192A2/en not_active Ceased
-
2016
- 2016-11-22 IL IL249126A patent/IL249126B/en active IP Right Grant
-
2019
- 2019-07-29 US US16/524,527 patent/US11486002B2/en active Active
-
2021
- 2021-08-27 JP JP2021138555A patent/JP2022001050A/ja active Pending
-
2022
- 2022-03-18 AU AU2022201907A patent/AU2022201907B2/en not_active Expired - Fee Related
- 2022-10-07 US US18/045,032 patent/US20230295718A1/en active Pending
-
2024
- 2024-03-13 JP JP2024038531A patent/JP2024060054A/ja active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3152331T3 (da) | Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner | |
| IL324299A (en) | Genomic infrastructure for on-site or cloud-based DNA and RNA processing and analysis | |
| IL269415A (en) | Devices, processes, and methods for determining nucleic acid sequence, expression, copy number, or methylation changes using reactions that combine nuclease, ligase, polymerase, and sequencing | |
| IL259788A (en) | Adapter nucleic acid sequences, compositions comprising same and methods for duplex sequencing using same | |
| DK3204520T3 (da) | Fremgangsmåde til identificering og kvantificering af nukleinsyreekspression, splejsningsvariant, translokation, kopiantal eller methyleringsændringer | |
| DK3456846T3 (da) | Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre | |
| IL259736A (en) | Methods and systems for nucleic acid analysis | |
| SG11201609211VA (en) | Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same | |
| DK3348636T3 (da) | Fremgangsmåde til modificering af en genomsekvens, der specifikt omdanner nukleobasen af en targetteret DNA-sekvens, og molekylær sekvens anvendt i fremgangsmåden | |
| DK3408407T3 (da) | Fremgangsmåder til påvisning af cpg-methylering af tumorafledt dna i blodprøver | |
| DK3390632T3 (da) | Fremgangsmåder og sammensætninger til polymerase ii (pol-ii)-baseret guide-rna-ekspression | |
| IL246615B (en) | Method for generating double stranded dna libraries and sequencing methods for the identification of methylated cytosines | |
| DK3386550T3 (da) | Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer | |
| EP3192900A4 (en) | Method for constructing nucleic acid single-stranded cyclic library and reagents thereof | |
| EP3194627C0 (en) | METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID SEQUENCING DATA | |
| EP3274293A4 (en) | Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or dna methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions | |
| SG11202002516WA (en) | Systems and methods for nucleic acid sequencing | |
| EP3192901A4 (en) | Method for constructing nucleic acid single-stranded cyclic library and reagents thereof | |
| DK3224374T3 (da) | Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering | |
| EP3102677A4 (en) | Genomic dna extraction reagent and method | |
| SG11201704744QA (en) | Methods and kits for purifying plasmid dna | |
| EP4209597C0 (en) | NUCLEIC ACID SEQUENCING PROCESS | |
| EP3124608A4 (en) | Method and reagent for extracting nucleic acid | |
| DK3208335T3 (da) | Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens | |
| HK40022886A (en) | Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions |