DK3152331T3 - Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner - Google Patents

Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner Download PDF

Info

Publication number
DK3152331T3
DK3152331T3 DK15802743T DK15802743T DK3152331T3 DK 3152331 T3 DK3152331 T3 DK 3152331T3 DK 15802743 T DK15802743 T DK 15802743T DK 15802743 T DK15802743 T DK 15802743T DK 3152331 T3 DK3152331 T3 DK 3152331T3
Authority
DK
Denmark
Prior art keywords
ligase
polymerase
copy
identifying
expression
Prior art date
Application number
DK15802743T
Other languages
English (en)
Inventor
Francis Barany
John William Efcavitch
Original Assignee
Univ Cornell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cornell filed Critical Univ Cornell
Application granted granted Critical
Publication of DK3152331T3 publication Critical patent/DK3152331T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
DK15802743T 2014-06-06 2015-06-08 Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner DK3152331T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462009047P 2014-06-06 2014-06-06
US201562136093P 2015-03-20 2015-03-20
PCT/US2015/034724 WO2015188192A2 (en) 2014-06-06 2015-06-08 Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions

Publications (1)

Publication Number Publication Date
DK3152331T3 true DK3152331T3 (da) 2019-11-04

Family

ID=54767613

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15802743T DK3152331T3 (da) 2014-06-06 2015-06-08 Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner

Country Status (12)

Country Link
US (3) US10407722B2 (da)
EP (4) EP4170044A1 (da)
JP (3) JP7008407B2 (da)
CN (2) CN106661631B (da)
AU (2) AU2015269103B2 (da)
CA (2) CA2950295C (da)
DK (1) DK3152331T3 (da)
ES (3) ES2864531T3 (da)
IL (1) IL249126B (da)
PT (3) PT3889271T (da)
SG (2) SG11201610134PA (da)
WO (1) WO2015188192A2 (da)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11859246B2 (en) * 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
PT3889271T (pt) * 2014-06-06 2022-12-20 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
US11085073B2 (en) * 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
US11220705B2 (en) 2015-04-24 2022-01-11 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
EP3380607B1 (en) 2015-11-25 2021-10-27 F. Hoffmann-La Roche AG Purification of polymerase complexes
CN108699505A (zh) * 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
US20210214781A1 (en) * 2016-02-14 2021-07-15 Abhijit Ajit Patel Measurement of nucleic acid
AU2017246318B2 (en) * 2016-04-07 2023-07-27 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free DNA
US20190185913A1 (en) * 2016-07-01 2019-06-20 Natera, Inc. Compositions and methods for detection of nucleic acid mutations
EP3532635B1 (en) * 2016-10-31 2021-06-09 F. Hoffmann-La Roche AG Barcoded circular library construction for identification of chimeric products
EP3559269B1 (en) * 2016-12-20 2020-09-16 F. Hoffmann-La Roche AG Single stranded circular dna libraries for circular consensus sequencing
CN109234388B (zh) * 2017-07-04 2021-09-14 深圳华大生命科学研究院 用于dna高甲基化区域富集的试剂、富集方法及应用
JP2020532976A (ja) * 2017-09-14 2020-11-19 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 環状一本鎖ｄｎａライブラリーを生成するための新規な方法
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
JP7304852B2 (ja) 2017-11-03 2023-07-07 ガーダント ヘルス， インコーポレイテッド 脱アミノ化に誘導される配列エラーの補正
US20210317516A1 (en) * 2017-11-27 2021-10-14 Bgi Shenzhen Method for constructing library of cell-free dnas in body fluids and application thereof
EP3724353A1 (en) 2017-12-11 2020-10-21 UMC Utrecht Holding B.V. Methods for preparing nucleic acid molecules for sequencing
JP7013960B2 (ja) * 2018-03-13 2022-02-01 株式会社リコー 核酸検出方法及び核酸検出装置、並びに、核酸検出キット
EP3775272A4 (en) 2018-04-02 2021-12-29 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CN112236520A (zh) * 2018-04-02 2021-01-15 格里尔公司 甲基化标记和标靶甲基化探针板
CN110456034B (zh) * 2018-05-07 2022-07-22 上海市第十人民医院 一种循环肿瘤细胞的检测方法
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10876148B2 (en) * 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
WO2020102766A2 (en) * 2018-11-15 2020-05-22 Element Biosciences, Inc. Methods for generating circular nucleic acid molecules
CN113811391A (zh) * 2019-02-25 2021-12-17 艾勒根公司 使用微流体位置编码设备的方法
JP7344973B2 (ja) * 2019-03-01 2023-09-14 マーシー バイオアナリティクス， インコーポレイテッド 標的実体を検出するためのシステム、組成物、及び方法
WO2020180813A1 (en) * 2019-03-06 2020-09-10 Qiagen Sciences, Llc Compositions and methods for adaptor design and nucleic acid library construction for rolony-based sequencing
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
BR112022001539A2 (pt) * 2019-05-13 2022-03-22 Rapid Genomics Llc Captura e análise de regiões genômicas alvo
CN112029841B (zh) * 2019-06-03 2024-02-09 香港中文大学 定量端粒长度和基因组基序的方法
CN112175939A (zh) * 2019-07-03 2021-01-05 华大青兰生物科技(无锡)有限公司 一种基于同源序列的核酸拼接方法及其应用
CN114616338A (zh) * 2019-08-22 2022-06-10 新加坡国立大学 生成哑铃形dna载体的方法
WO2021036995A1 (en) * 2019-08-23 2021-03-04 Nanjing University Direct microrna sequencing using enzyme assisted sequencing
CN114641581A (zh) * 2019-10-28 2022-06-17 深圳华大智造科技股份有限公司 Dna接头寡核苷酸
DE112020005433T5 (de) 2019-11-06 2022-10-06 The Board Of Trustees Of The Leland Stanford Junior University Verfahren und Systeme zur Analyse von Nukleinsäuremolekülen
CN111154820B (zh) * 2020-01-13 2021-12-21 上海韦翰斯生物医药科技有限公司 一种降低核酸扩增复制滑移率的方法
EP4114966A1 (en) * 2020-03-03 2023-01-11 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
WO2021185320A1 (en) * 2020-03-18 2021-09-23 Mgi Tech Co., Ltd. Restoring phase in massively parallel sequencing
US20230279483A1 (en) * 2022-03-04 2023-09-07 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
EP4001432A1 (en) * 2020-11-13 2022-05-25 Miltenyi Biotec B.V. & Co. KG Algorithmic method for efficient indexing of genetic sequences using associative arrays
WO2022101162A1 (en) * 2020-11-13 2022-05-19 Miltenyi Biotec B.V. & Co. KG Paired end sequential sequencing based on rolling circle amplification
CN112631562B (zh) * 2020-12-01 2022-08-23 上海欧易生物医学科技有限公司 基于python的二代测序样本混样方法、应用、设备、计算机可读存储介质
EP4060049B1 (en) * 2021-03-18 2023-08-23 Genomill Health Oy Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
CN113552191B (zh) * 2021-07-28 2023-11-21 江苏师范大学 基于多层dna放大回路检测甲基化dna的比例型电化学传感器的构建方法
WO2023122104A2 (en) * 2021-12-21 2023-06-29 Ultima Genomics, Inc. Systems and methods for library preparation adapters
IL293202A (en) * 2022-05-22 2023-12-01 Nucleix Ltd Useful combinations of restriction enzymes
WO2024084440A1 (en) * 2022-10-19 2024-04-25 Biofidelity Ltd Nucleic acid enrichment and detection

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5736373A (en) 1996-09-23 1998-04-07 Becton, Dickinson And Company Thermostable DNA polymerase from Bacillus pallidus
US20040086892A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
WO2006030455A1 (en) 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
US20080311626A1 (en) 2004-09-17 2008-12-18 Arkea Hf Dna Polymerases Having Strand Displacement Activity
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
WO2006119066A2 (en) * 2005-04-29 2006-11-09 The J. Craig Venter Institute Amplification and cloning of single dna molecules using rolling circle amplification
US20120083018A1 (en) 2005-10-06 2012-04-05 Schoenfeld Thomas W Thermostable dna polymerases and methods of use
CA2624324A1 (en) 2005-10-06 2007-04-19 Lucigen Corporation Thermostable viral polymerases and methods of use
WO2007076461A1 (en) 2005-12-22 2007-07-05 Ge Healthcare Bio-Sciences Corp. Thermostable dna polymerase from thermus scotoductus
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
DE102006020885A1 (de) 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
JP4962899B2 (ja) 2006-08-31 2012-06-27 東洋製罐株式会社 環状１本鎖ｄｎａの製造方法
US20080096258A1 (en) * 2006-10-24 2008-04-24 Christian Korfhage Rolling circle amplification of circular genomes
WO2010120803A2 (en) * 2009-04-13 2010-10-21 Somagenics Inc. Methods and compositions for detection of small rnas
CA2760439A1 (en) * 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US9249192B2 (en) * 2009-08-21 2016-02-02 Virginia Tech Intellectual Properties, Inc. Infectious genomic DNA clone and serological profile of Torque teno sus virus 1 and 2
EP4212630A1 (en) * 2009-11-06 2023-07-19 The Chinese University of Hong Kong Size-based genomic analysis
WO2012003374A2 (en) * 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
GB201018714D0 (en) 2010-11-05 2010-12-22 Oxitec Ltd Strand displacement activity of modified polymerases and uses thereof
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
EP2718866B1 (en) * 2011-06-07 2020-09-09 Koninklijke Philips N.V. Providing nucleotide sequence data
US9670540B2 (en) 2011-07-21 2017-06-06 Cornell University Methods and devices for DNA sequencing and molecular diagnostics
CA2848304A1 (en) * 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior University Methods for sequencing a polynucleotide
WO2013188840A1 (en) * 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2898096B1 (en) * 2012-09-21 2024-02-14 The Broad Institute, Inc. Methods for labeling of rnas
US10017820B2 (en) * 2013-03-05 2018-07-10 Agilent Technologies, Inc. Detection of genomic rearrangements by sequence capture
US10202629B2 (en) * 2013-03-15 2019-02-12 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing clamp oligonucleotides
WO2014161712A1 (en) 2013-04-05 2014-10-09 Bioron Gmbh Novel dna-ploymerases
CN104946737B (zh) * 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
PT3889271T (pt) * 2014-06-06 2022-12-20 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas

Also Published As

Publication number Publication date
IL249126A0 (en) 2017-01-31
SG10201810862YA (en) 2019-01-30
US20230295718A1 (en) 2023-09-21
JP2017516487A (ja) 2017-06-22
EP3152331A4 (en) 2018-05-09
CN106661631A (zh) 2017-05-10
AU2015269103A1 (en) 2016-12-15
JP7008407B2 (ja) 2022-01-25
JP2022001050A (ja) 2022-01-06
EP4170044A1 (en) 2023-04-26
CA2950295A1 (en) 2015-12-10
US11486002B2 (en) 2022-11-01
CA2950295C (en) 2023-10-17
CA3213538A1 (en) 2015-12-10
AU2022201907A1 (en) 2022-04-07
US20170204459A1 (en) 2017-07-20
US10407722B2 (en) 2019-09-10
WO2015188192A3 (en) 2016-05-26
IL249126B (en) 2020-07-30
EP3567122A1 (en) 2019-11-13
PT3567122T (pt) 2021-04-07
CN113046413A (zh) 2021-06-29
EP3889271A1 (en) 2021-10-06
EP3152331B1 (en) 2019-08-07
SG11201610134PA (en) 2017-01-27
ES2748457T3 (es) 2020-03-16
US20190345552A1 (en) 2019-11-14
ES2864531T3 (es) 2021-10-14
PT3889271T (pt) 2022-12-20
EP3567122B1 (en) 2021-02-17
EP3152331A2 (en) 2017-04-12
JP2024060054A (ja) 2024-05-01
EP3889271B1 (en) 2022-11-23
AU2015269103B2 (en) 2021-12-23
ES2935285T3 (es) 2023-03-03
CN106661631B (zh) 2021-04-02
PT3152331T (pt) 2019-11-05
WO2015188192A2 (en) 2015-12-10

Similar Documents

Publication Publication Date Title
DK3152331T3 (da) Fremgangsmåde til identifikation og optælling af nukleinsyresekvens, ekspression, kopi eller DNA-metyleringsændringer under anvendelse af kombineret nuklease, ligase, polymerase og sekventeringsreaktioner
IL286496A (en) A method for the detection and relative quantification of nucleic acid sequence, expression, variants, transition, copy number, or methylation changes, using a combination of nuclease, ligase, and polymerase reactions with protection against contamination
IL269415A (en) Devices, processes, and methods for determining nucleic acid sequence, expression, copy number, or methylation changes using reactions that combine nuclease, ligase, polymerase, and sequencing
ZA202210297B (en) Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis
DK3456846T3 (da) Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
IL259788A (en) Adapted nucleic acid sequences, preparations containing them and methods for determining duplex sequence using them
IL259736A (en) Methods and systems for nucleic acid analysis
DK3348636T3 (da) Fremgangsmåde til modificering af en genomsekvens, der specifikt omdanner nukleobasen af en targetteret DNA-sekvens, og molekylær sekvens anvendt i fremgangsmåden
EP3194627C0 (en) METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACID SEQUENCING DATA
SG11201609211VA (en) Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
BR112017008877A2 (pt) métodos e composições para sequenciamento de ácido nucleico-alvo
DK3386550T3 (da) Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer
DK3408407T3 (da) Fremgangsmåder til påvisning af cpg-methylering af tumorafledt dna i blodprøver
EP3192900A4 (en) Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
GB2550712B (en) Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing
EP3274293A4 (en) Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or dna methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions
IL273426A (en) Systems and methods for nucleic acid sequencing
EP3192901A4 (en) Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
DK3224374T3 (da) Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering
DK3119886T3 (da) Kopiantalsbevarende RNA-analysefremgangsmåde
EP3102677A4 (en) Genomic dna extraction reagent and method
SG11201704744QA (en) Methods and kits for purifying plasmid dna
DK3208335T3 (da) Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens
EP3124608A4 (en) Method and reagent for extracting nucleic acid
HUE061474T2 (hu) Nukleinsav-szekvenálási eljárás