DK3080243T3 - Anvendelse af enzymer, som katalyserer pyruvatsyntese ud fra formiat og acetyl-coa, samt bakterier, der udtrykker dette - Google Patents

Anvendelse af enzymer, som katalyserer pyruvatsyntese ud fra formiat og acetyl-coa, samt bakterier, der udtrykker dette Download PDF

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DK3080243T3
DK3080243T3 DK14869116.5T DK14869116T DK3080243T3 DK 3080243 T3 DK3080243 T3 DK 3080243T3 DK 14869116 T DK14869116 T DK 14869116T DK 3080243 T3 DK3080243 T3 DK 3080243T3
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formate
microorganism
pyruvate
lyase
acetate
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DK14869116.5T
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Ron Milo
Elad Noor
Lior Zelcbuch
Arren Bar-Even
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Yeda Res & Dev
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
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    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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Claims (13)

  1. ANVENDELSE AF ENZYMER, SOM KATALYSERER PYRUVATSYNTESE UD FRA FORMIAT OG ACETYL-COA, SAMT BAKTERIER, DER UDTRYKKER DETTE Patentkrav
    1. Isoleret mikroorganisme, der er genetisk modificeret til at udtrykke pyruvatformiatlyase (PFL) eller 2-ketobutyratformiatlyase, hvor det gen, der koder for isocitratlyase (aceA) og/eller malatsynthase i mikroorganismen, deleteres, hvor acetyl-CoA i mikroorganismen konverteres til pyruvat i nærværelse af formiat i enkelttrinsreaktion, hvor reaktionens nettoflux er i retning af pyruvatsyntese.
  2. 2. Isoleret mikroorganisme ifølge krav 1, hvor pyruvatformiatlyasen kodes af et gen, der er valgt fra gruppen bestående af pflB, pfID og ybiW.
  3. 3. Isoleret mikroorganisme ifølge krav 1, der endvidere udtrykker et polypeptid, som aktiverer pyruvatformiatlyaseenzymet.
  4. 4. Mikroorganisme ifølge et hvilket som helst af kravene 1-3, der erformiatotrof, autotrof eller methyltrof.
  5. 5. Mikroorganisme ifølge et hvilket som helst af kravene 1-4, der er en bakterie, en gær eller en svamp.
  6. 6. Mikroorganisme ifølge et hvilket som helst af kravene 1-5, der er genetisk modificeret til at udtrykke et humant polypeptid.
  7. 7. Mikroorganisme ifølge et hvilket som helst af kravene 1 -5, der er i stand til at fremstille et biobrændsel.
  8. 8. Cellekultur, der omfatter den isolerede mikroorganisme ifølge et hvilket som helst af kravene 1-7 og et medium, som er egnet til opformering af mikroorganismen.
  9. 9. Cellekultur ifølge krav 8, hvor mediet omfatter en mængde acetat og/eller formiat, som fremmer reaktionens nettoflux til at være i retning af pyruvatsyntese.
  10. 10. Fremgangsmåde til generering af et biobrændsel, hvilken fremgangsmåde omfatter dyrkning af mikroorganismen ifølge krav 7 under betingelser, der tillader dannelse af biobrændsel, hvorved biobrændslet genereres.
  11. 11. Fremgangsmåde til generering af et humant polypeptid, hvilken fremgangsmåde omfatter dyrkning af mikroorganismen ifølge krav 6 under betingelser, der tillader dannelse af biobrændsel, hvorved biobrændslet genereres.
  12. 12. Fremgangsmåde til fjernelse af acetat fra et acetatholdigt medium, hvilken fremgangsmåde omfatter, at mikroorganismen ifølge krav 1 bringes i kontakt med mediet, hvorved acetatet fjernes fra det acetatholdige medium.
  13. 13. Fremgangsmåde til fjernelse af formiat fra et formiatholdigt medium, hvilken fremgangsmåde omfatter, at mikroorganismen ifølge krav 1 bringes i kontakt med mediet, hvorved formiatet fjernes fra det formiatholdige medium.
DK14869116.5T 2013-12-10 2014-12-10 Anvendelse af enzymer, som katalyserer pyruvatsyntese ud fra formiat og acetyl-coa, samt bakterier, der udtrykker dette DK3080243T3 (da)

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US201361913940P 2013-12-10 2013-12-10
PCT/IL2014/051080 WO2015087327A1 (en) 2013-12-10 2014-12-10 Use of enzymes which catalyze pyruvate synthesis from formate and acetyl-coa and bacteria expressing same

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ES2697757T3 (es) 2013-12-10 2019-01-28 Yeda Res & Dev Uso de enzimas que catalizan la síntesis de piruvato a partir de formiato y acetil-CoA y bacterias que expresan la misma
CN107406818A (zh) * 2014-12-30 2017-11-28 诺沃吉公司 增强产油酵母中核心脂质的生产
JP2019165636A (ja) * 2016-08-12 2019-10-03 Ａｇｃ株式会社 形質転換体および３−ヒドロキシプロピオン酸の製造方法
WO2021084526A1 (en) 2019-10-31 2021-05-06 Yeda Research And Development Co. Ltd. Engineered autotrophic bacteria for co2 conversion to organic materials
CN113512567B (zh) * 2021-06-29 2023-05-23 安徽师范大学 一种提高光异养体系微藻产油率的方法

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US10053712B2 (en) 2018-08-21
EP3080243B1 (en) 2018-09-05
EP3080243A4 (en) 2017-05-24
EP3080243A1 (en) 2016-10-19
ES2697757T3 (es) 2019-01-28
US20170081682A1 (en) 2017-03-23

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