DK3054298T3 - Fremgangsmåde til detektering af tumor i bugspytkirtlen - Google Patents
Fremgangsmåde til detektering af tumor i bugspytkirtlen Download PDFInfo
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- DK3054298T3 DK3054298T3 DK14850747.8T DK14850747T DK3054298T3 DK 3054298 T3 DK3054298 T3 DK 3054298T3 DK 14850747 T DK14850747 T DK 14850747T DK 3054298 T3 DK3054298 T3 DK 3054298T3
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Claims (8)
1. Fremgangsmåde til detektering af kræft i bugspytkirtlen eller godartet tumor i bugspytkirtlen ved at måle mængderne af apolipoprotein A2 (APOA2)-proteinvarianter i en kropsvæskeprøve fra en forsøgsperson, idet fremgangsmåden til detektering omfatter: (A) et første trin til måling af mængden af APOA2-ATQ-protein i prøven under anvendelse af et anti-APOA2-ATQ-terminus antistof der specifikt binder til en C-terminal region af APOA2-ATQ-proteinet omfattende aminosyresekvensen repræsenteret ved SEQ ID NO: 1, og et anti-APOA2-ATQ ikke-terminus antistof der binder til aminosyresekvensen bortset fra den C-terminale region; (B) et andet trin til måling af mængden af APOA2-AT-protein i prøven under anvendelse af et anti-APOA2-AT-terminus antistof der specifikt binder til en C-terminal region af APOA2-AT-proteinet omfattende aminosyresekvensen repræsenteret ved SEQ ID NO: 2 og et anti-APOA2-AT ikke-terminus antistof der binder til aminosyresekvensen bortset fra den C-terminal region; og (C) et tredje trin til indlæsning, til en forud indstillet diskriminant, af målingsværdien af mængden af APOA2-ATQ-protein opnået i det første trin og målingsværdien af mængden af APOA2-AT-protein opnået i det andet trin og til at bestemme om forsøgspersonen har kræft i bugspytkirtlen eller en godartet tumor i bugspytkirtlen, når den resulterende diskriminantværdi hos forsøgspersonen er statistisk signifikant anderledes sammenlignet med diskriminantværdien hos en normal person, hvor APOA2-ATQ-proteinet er fuldlængde APOA2-proteinet og APOA2-AT proteinet er APOA2-proteinet, som mangler den C-terminale glutaminrest.
2. Fremgangsmåde til detektering ifølge krav 1, hvor de C-terminale regioner af APOA2-ATQ-proteinet og APOA2-AT-proteinet hver består af en sekvens omfattende 6 eller flere fortløbende aminosyrer inklusiv C terminuset.
3. Fremgangsmåde til detektering ifølge krav 1 eller 2, hvor diskriminanten er en hvilken som helst valgt fra gruppen bestående af en logistisk regression ekspression, en ekspression udarbejdet ved analyse med en supportvektormaskine, en ekspression udarbejdet ved analyse af et nervenetværk og en ekspression udarbejdet ved diskriminantanalyse.
4. Fremgangsmåde til detektering ifølge krav 3, hvor den logistiske regression ekspression omfatter, som en variable, måleværdien af APOA2-ATQ-proteinet, måleværdien af APOA2-AT-proteinet, og/eller produktet af måleværdien af APOA2-ATQ-proteinet og måleværdien af APOA2-AT-proteinet.
5. Fremgangsmåde ifølge krav 4, hvor diskriminantværdien af forsøgspersonen opnået fra den logistiske regression ekspression er 2/3 eller lavere end diskriminantværdien hos en normal person.
6. Fremgangsmåde til detektering ifølge et hvilket som helst af kravene 1 til 5, yderligere omfattende et fjerde trin til måling af mængden af bugspytkirtel kræftmarkør CA19-9 eller DU-PAN-2 i en kropsvæskeprøve fra forsøgspersonen bestemt til at have kræft i bugspytkirtlen eller en godartet tumor i bugspytkirtlen i det tredje trin, og til at bestemme at forsøgspersonen har kræft i bugspytkirtlen, når måleværdien overstiger en forudbestemt referenceværdi og til at bestemme at forsøgspersonen har en godartet tumor i bugspytkirtlen, når måleværdien er lig med eller mindre end referenceværdien.
7. Fremgangsmåde til detektering ifølge et hvilket som helst af kravene 1 til 6, hvor kropsvæskeprøven er blod, plasma eller serum.
8. Fremgangsmåde til detektering ifølge et hvilket som helst af kravene 1 til 7, hvor kræften i bugspytkirtlen er tidlig kræft i bugspytkirtlen.
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| PCT/JP2014/076035 WO2015050107A1 (ja) | 2013-10-01 | 2014-09-30 | 膵腫瘍の検出方法、抗体及び膵腫瘍検出用キット |
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| WO2016140210A1 (ja) | 2015-03-02 | 2016-09-09 | 東レ株式会社 | 膵臓機能異常の検出方法及び検出用キット |
| GB201608192D0 (en) * | 2016-05-10 | 2016-06-22 | Immunovia Ab | Method, array and use thereof |
| WO2018139608A1 (ja) * | 2017-01-27 | 2018-08-02 | 株式会社Ａ－Ｃｌｉｐ研究所 | 感染性疾患または炎症性疾患の予防および／または治療剤 |
| WO2019131552A1 (ja) * | 2017-12-27 | 2019-07-04 | 国立大学法人熊本大学 | 膵がん等のがんの判定用バイオマーカー |
| TWI709188B (zh) | 2018-09-27 | 2020-11-01 | 財團法人工業技術研究院 | 基於機率融合的分類器、分類方法及分類系統 |
| CN111863129A (zh) * | 2020-07-07 | 2020-10-30 | 南京世和基因生物技术股份有限公司 | 一种脑脊液中突变的检测方法及检测装置 |
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| US5714350A (en) | 1992-03-09 | 1998-02-03 | Protein Design Labs, Inc. | Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region |
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| JP2001289861A (ja) | 2000-04-04 | 2001-10-19 | Tsuneo Kobayashi | 腫瘍マーカーによる癌の評価方法 |
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| AU2006203948A1 (en) * | 2005-01-06 | 2006-07-13 | Eastern Virginia Medical School | Apolipoprotein A-II isoform as a biomarker for prostate cancer |
| JP5200246B2 (ja) | 2005-03-14 | 2013-06-05 | 財団法人ヒューマンサイエンス振興財団 | 膵臓癌診断用マーカータンパク質 |
| WO2007133957A1 (en) * | 2006-05-09 | 2007-11-22 | Vermillion, Inc. | Biomarkers for mesothelioma: apoc1 and apoa2 |
| FR2919064B1 (fr) * | 2007-07-19 | 2009-10-02 | Biomerieux Sa | Procede de dosage de l'apolipoproteine all pour le diagnostic in vitro du cancer colorectal |
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| JP2010175452A (ja) * | 2009-01-30 | 2010-08-12 | Japan Health Science Foundation | 膵臓癌の検出法 |
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| EP3054298B1 (en) | 2019-02-20 |
| CA2925099C (en) | 2023-04-04 |
| CA3189270A1 (en) | 2015-04-09 |
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| BR112016007037A2 (pt) | 2017-12-12 |
| CA2925099A1 (en) | 2015-04-09 |
| JPWO2015050107A1 (ja) | 2017-03-09 |
| RU2016116773A3 (da) | 2018-07-23 |
| RU2693006C2 (ru) | 2019-07-01 |
| EP3054298A1 (en) | 2016-08-10 |
| RU2016116773A (ru) | 2017-11-14 |
| US20160245815A1 (en) | 2016-08-25 |
| CN105637366A (zh) | 2016-06-01 |
| JP6519056B2 (ja) | 2019-05-29 |
| WO2015050107A1 (ja) | 2015-04-09 |
| EP3054298A4 (en) | 2017-08-02 |
| CN105637366B (zh) | 2018-05-29 |
| CA3189276A1 (en) | 2015-04-09 |
| KR20160065876A (ko) | 2016-06-09 |
| BR112016007037B1 (pt) | 2023-12-26 |
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