DK2885407T3 - Nedbrydende enzymer fra macrophomina phaseolina og anvendelser deraf - Google Patents

Nedbrydende enzymer fra macrophomina phaseolina og anvendelser deraf Download PDF

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DK2885407T3
DK2885407T3 DK13829902.9T DK13829902T DK2885407T3 DK 2885407 T3 DK2885407 T3 DK 2885407T3 DK 13829902 T DK13829902 T DK 13829902T DK 2885407 T3 DK2885407 T3 DK 2885407T3
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Denmark
Prior art keywords
seq
phaseolina
sequence
polypeptide
isolated
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DK13829902.9T
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English (en)
Inventor
Maqsudul Alam
Mohammed Shahidul Islam
Mohammed Mosaddeque Hossen
Mohammed Samiul Haque
Mohammed Monjurul Alam
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Bangladesh Jute Res Institute
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011—Pectinesterase (3.1.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002—Pectate lyase (4.2.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02023—Rhamnogalacturonan endolyase (4.2.2.23)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Textile Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Claims (15)

1. Isoleret polynukleotidsekvens, der omfatter en nukleotidsekvens, der er mindst 80 % identisk med nukleotidsekvensen ifølge SEQ ID NO: 1 eller 2, eller komplementet deraf, hvor polynukleotidet koder for en pectatlyase.
2. Isoleret nukleinsyresonde, der hybridiserer under medium stringente forhold til en isoleret polynukleotidsekvens, der omfatter en nukleotidsekvens ifølge SEQ ID NO: 1 eller 2, eller komplementet deraf, hvor polynukleotidet koder for en pectatlyase, og hvor de medium stringente forhold indbefatter hybridisering til filterbundet DNA i 6x natriumchlorid/natriumcitrat (SSC) ved ca. 45 °C efterfulgt af én eller flere gange vask i 0,2xSSC/0,l % SDS ved ca. 50 til ca. 65 °C.
3. Isoleret polynukleotidsekvens ifølge krav 1 eller isoleret nukleinsyresonde ifølge krav 2, der er genomisk DNA.
4. Isoleret polynukleotidsekvens ifølge krav 1 eller isoleret nukleinsyresonde ifølge krav 2, der er cDNA.
5. Isoleret polynukleotidsekvens ifølge krav 1 eller isoleret nukleinsyresonde ifølge krav 2, der er RNA.
6. Isoleret polynukleotidsekvens ifølge krav 1 eller isoleret nukleinsyresonde ifølge krav 2, der er enkeltstrenget.
7. Nukleinsyremolekyle, der omfatter isoleret polynukleotidsekvens ifølge krav 1 uafbrudt af stopkodon i en kodningssekvens, der koder for et heterologt protein eller peptid.
8. Rekombinant vektor, der omfatter isoleret polynukleotidsekvens ifølge krav 1.
9. Ekspressionskonstrukt, der omfatter isoleret polynukleotidsekvens ifølge krav 1, hvor polynukleotidsekvensen er operativt forbundet med en regulatorisk nukleotidsekvens, der indeholder regulatoriske transskriptions- eller translationssignaler, eller begge, der styrer ekspression af nukleotidsekvensen i en værtscelle.
10. Genetisk modificeret værtscelle, der omfatter isoleret polynukleotidsekvens ifølge krav 1.
11. Fremgangsmåde til fremstilling af et polypeptid omfattende følgende trin: i. dyrkning af en celle transformeret med et ekspressionskonstrukt ifølge krav 9 under forhold, der muliggør fremstilling af polypeptidet og ii. isolering af polypeptidet.
12. Isoleret polypeptid, der omfatter en aminosyresekvens der er mindst 80 % identisk med en aminosyresekvens ifølge SEQ ID NO: 3 og udviser pectatlyases katalytiske aktivitet.
13. Kimærisk protein, der omfatter et polypeptid ifølge krav 12 kondenseret via en kovalent binding til en aminosyresekvens af et andet polypeptid.
14. Værtscelle, der omfatter ekspressionskonstruktet ifølge krav 9.
15. Transgen svamp af M. phaseolina, der omfatter ekspressionskonstruktet ifølge krav 9.
DK13829902.9T 2012-08-16 2013-08-15 Nedbrydende enzymer fra macrophomina phaseolina og anvendelser deraf DK2885407T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261683914P 2012-08-16 2012-08-16
PCT/US2013/055198 WO2014028772A2 (en) 2012-08-16 2013-08-15 Pectin degrading enzymes from macrophomina phaseolina and uses thereof

Publications (1)

Publication Number Publication Date
DK2885407T3 true DK2885407T3 (da) 2018-07-16

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DK13829902.9T DK2885407T3 (da) 2012-08-16 2013-08-15 Nedbrydende enzymer fra macrophomina phaseolina og anvendelser deraf

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US (1) US9765322B2 (da)
EP (1) EP2885407B1 (da)
JP (1) JP6644547B2 (da)
KR (1) KR102043363B1 (da)
CN (1) CN104837991A (da)
AU (1) AU2013302535B2 (da)
DK (1) DK2885407T3 (da)
ES (1) ES2674922T3 (da)
HK (1) HK1213597A1 (da)
IN (1) IN2015DN02049A (da)
MY (1) MY169809A (da)
PL (1) PL2885407T3 (da)
PT (1) PT2885407T (da)
TR (1) TR201809053T4 (da)
WO (1) WO2014028772A2 (da)
ZA (1) ZA201501635B (da)

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
ES2063162T5 (es) 1988-07-28 2002-11-16 Novartis Ag Nuevo sistema de expresion.
DE3908813A1 (de) 1989-03-17 1990-09-20 Roehm Gmbh Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus
WO1994014952A1 (en) 1992-12-23 1994-07-07 Novo Nordisk A/S An enzyme with polygalacturonase activity
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
EP1683860B1 (en) * 1995-03-17 2013-10-23 Novozymes A/S Novel endoglucanases
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
CN1137254C (zh) 1997-04-09 2004-02-04 花王株式会社 洗涤剂组合物
US7214786B2 (en) * 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
AR040004A1 (es) * 2002-05-14 2005-03-09 Novozymes As Variantes de pectatoliasa
EP1507857A1 (en) * 2002-05-30 2005-02-23 DSM IP Assets B.V. Novel pectinases and uses thereof
CN102016041A (zh) * 2008-02-29 2011-04-13 中央佛罗里达大学研究基金会有限公司 植物降解材料的合成和应用
AR074835A1 (es) * 2008-12-19 2011-02-16 Danisco Proceso para la produccion de un producto de enzimas mediante la fermentacion sumergida con aspergillus
WO2010101158A1 (ja) * 2009-03-02 2010-09-10 住友商事株式会社 クロストリジウム セルロボランス由来新規遺伝子及びその利用

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Publication number Publication date
US20150259667A1 (en) 2015-09-17
PT2885407T (pt) 2018-07-02
MY169809A (en) 2019-05-16
WO2014028772A3 (en) 2014-05-08
KR20150042849A (ko) 2015-04-21
AU2013302535A1 (en) 2015-03-26
JP6644547B2 (ja) 2020-02-12
BR112015003086A2 (pt) 2018-06-26
EP2885407A2 (en) 2015-06-24
AU2013302535B2 (en) 2018-12-06
US9765322B2 (en) 2017-09-19
ES2674922T3 (es) 2018-07-05
WO2014028772A2 (en) 2014-02-20
JP2015529071A (ja) 2015-10-05
TR201809053T4 (tr) 2018-07-23
KR102043363B1 (ko) 2019-11-11
HK1213597A1 (zh) 2016-07-08
EP2885407B1 (en) 2018-06-13
CN104837991A (zh) 2015-08-12
IN2015DN02049A (da) 2015-08-14
EP2885407A4 (en) 2016-05-11
PL2885407T3 (pl) 2018-09-28
ZA201501635B (en) 2016-01-27

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