DK2836588T3 - Biobeskyttelse under anvendelse af Lactobacillus paracasei-stammer - Google Patents

Biobeskyttelse under anvendelse af Lactobacillus paracasei-stammer Download PDF

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DK2836588T3
DK2836588T3 DK13717237.5T DK13717237T DK2836588T3 DK 2836588 T3 DK2836588 T3 DK 2836588T3 DK 13717237 T DK13717237 T DK 13717237T DK 2836588 T3 DK2836588 T3 DK 2836588T3
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strain
product
lactobacillus
lactobacillus paracasei
cfu
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DK13717237.5T
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Silja Kej Diemer
Tina Hornbaek
Maike Lisberg
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Chr Hansen As
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Priority claimed from PCT/EP2012/056384 external-priority patent/WO2012136830A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C13/00—Cream; Cream preparations; Making thereof
    • A23C13/12—Cream preparations
    • A23C13/16—Cream preparations containing, or treated with, microorganisms, enzymes, or antibiotics; Sour cream
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0323—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/076—Soft unripened cheese, e.g. cottage or cream cheese
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3571—Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/165—Paracasei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/175—Rhamnosus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/853—Lactobacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
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  • Animal Husbandry (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)

Claims (15)

1. Lactobacillus paracasei-stamme, som er valgt fra gruppen bestående af Lactobacillus paracasei-stammen CHCC14676, som blev deponeret hos German Collection of Microorganisms and Cell Cultures (DSMZ) under deponeringsnummer DSM25612, og mutantstammer afledt deraf, hvor mutantstammerne har i det væsentlige de samme eller forbedrede antifungale egenskaber i forhold til den stamme, der er deponeret under deponeringsnummer DSM25612.
2. Antifungalt præparat, som omfatter mindst én Lactobacillus paracasei-stamme ifølge krav 1.
3. Antifungalt præparat ifølge krav 2, som desuden omfatter mindst én stamme af Lactobacillus rhamnosus.
4. Antifungalt præparat ifølge krav 3, hvor den mindst ene stamme af Lactobacillus rhamnosus er valgt fra gruppen bestående af Lactobacillus rhamnosus CHCC12697 med deponeringsnummer DSM24616, Lactobacillus rhamnosus CHCC14226 med deponeringsnummer DSM24652, Lactobacillus rhamnosus CHCC5366 med deponeringsnummer DSM23035 og mutantstammer afledt deraf, hvor mutantstammerne har i det væsentlige de samme eller forbedrede antifungale egenskaber i forhold til én af de stammer, der er deponeret under deponeringsnummer DSM24616, DSM24652 eller DSM23035.
5. Fødevareprodukt, foderprodukt eller farmaceutisk produkt, som omfatter en Lactobacillus paracasei-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4.
6. Fødevareprodukt ifølge krav 5, som omfatter en mængde af en Lactobacillus paracasei-stamme ifølge krav 1, som er effektiv til at bibringe fødevareproduktet antifungale egenskaber.
7. Fødevareprodukt ifølge krav 5 eller 6, hvor fødevareproduktet er valgt fra gruppen bestående af frugter og frugtafledte produkter, grønsager og grønsagsafledte produk ter, korn og kornafledte produkter, mejeriprodukter, kød, fjerkræ samt fisk og skaldyr samt blandinger deraf.
8. Fødevareprodukt ifølge krav 7, hvor fødevareproduktet er et mejeriprodukt, og mejeriproduktet er et mesofilt eller et termofilt fermenteret mælkeprodukt, såsom yoghurt eller creme fraiche.
9. Fremgangsmåde til fremstilling af et fødevareprodukt, foderprodukt eller farmaceutisk produkt, hvilken fremgangsmåde omfatter tilsætning af mindst én Lactobacillus paracasei-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til fødevareproduktet, foderproduktet eller det farmaceutiske produkt under fremstilling og regulering af fremstillingsparametrene under fremstillingen således, at koncentrationen af den mindst ene Lactobacillus paracasei-stamme forbliver konstant eller øges.
10. Fremgangsmåde ifølge krav 9, hvor koncentrationen af den mindst ene Lactobacillus paracasei-stamme er mindst 1x106 cfu/g eller hver mindst 1x106 cfu/ml af fødevareproduktet, foderproduktet eller det farmaceutiske produkt eller mindst 1 x105 cfu/cm2 af overfladen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt.
11. Fremgangsmåde ifølge krav 9, som omfatter (a) tilsætning af det antifungale præparat ifølge et hvilket som helst af kravene 2 til 4 under fremstillingen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt, således at koncentrationen af den mindst ene stamme af Lactobacillus rhamnosus og/eller den mindst ene stamme af Lactobacillus paracasei hver er mindst 1x106 cfu/g eller mindst 1x106 cfu/ml af fødevareproduktet, foderproduktet eller det farmaceutiske produkt eller mindst 1x105 cfu/cm2 af overfladen af fødevareproduktet, foderproduktet eller det farmaceutiske produkt, og (b) regulering af fremstillingsparametrene under fremstillingen, således at koncentrationen af den mindst ene stamme af Lactobacillus rhamnosus og/eller den mindst ene stamme af Lactobacillus paracasei øges eller forbliver konstant.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 9 til 11, hvor fremgangsmåden omfatter ét eller flere fermenteringstrin.
13. Fremgangsmåde ifølge krav 12, hvor fremgangsmåden omfatter fermentering af et mælkesubstrat med en starterkultur, som omfatter mindst én stamme af de slægter, der er valgt blandt Lactobacillus, Streptococcus, Lactococcus og Leuconostoc.
14. Anvendelse af en Lactobacillus paracasei-stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til fremstilling af et fødevareprodukt, foderprodukt eller farmaceutisk produkt.
15. Ikke-medicinsk anvendelse afen Lactobacillus paracasei- stamme ifølge krav 1 eller et antifungalt præparat ifølge et hvilket som helst af kravene 2 til 4 til inhibering afvæksten af gær og skimmelsvampe.
DK13717237.5T 2012-04-09 2013-04-09 Biobeskyttelse under anvendelse af Lactobacillus paracasei-stammer DK2836588T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/EP2012/056384 WO2012136830A1 (en) 2011-04-08 2012-04-09 Synergistic antimicrobial effect
EP12163509 2012-04-09
EP12168971 2012-05-22
PCT/EP2013/057400 WO2013153070A1 (en) 2012-04-09 2013-04-09 Bioprotection using lactobacillus paracasei strains

Publications (1)

Publication Number Publication Date
DK2836588T3 true DK2836588T3 (da) 2017-05-22

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DK13717237.5T DK2836588T3 (da) 2012-04-09 2013-04-09 Biobeskyttelse under anvendelse af Lactobacillus paracasei-stammer

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US (2) US10059919B2 (da)
EP (1) EP2836588B1 (da)
JP (1) JP6430930B2 (da)
KR (1) KR101949372B1 (da)
CN (1) CN104254599B (da)
BR (1) BR112014024014B1 (da)
DK (1) DK2836588T3 (da)
EA (1) EA034173B1 (da)
ES (1) ES2623768T3 (da)
HK (1) HK1206390A1 (da)
HU (1) HUE034411T2 (da)
MX (1) MX351627B (da)
PL (1) PL2836588T3 (da)
PT (1) PT2836588T (da)
UA (1) UA115331C2 (da)
WO (1) WO2013153070A1 (da)

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EP3780962A2 (en) * 2018-04-19 2021-02-24 Chr. Hansen A/S Inhibition of fungal growth by manganese depletion
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WO2022115709A2 (en) * 2020-11-30 2022-06-02 January, Inc. Methods and composition for treating metabolic syndrome
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WO2023232677A1 (en) * 2022-05-31 2023-12-07 Lactobio A/S Strains, compositions and methods of use
NL2035175B1 (en) * 2023-03-01 2024-03-25 Chongqing Tianyou Dairy Co Ltd Lactobacillus paracasei ty-f15 and application thereof

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