DK2771460T3 - ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE - Google Patents

ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE Download PDF

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Publication number
DK2771460T3
DK2771460T3 DK12787268.7T DK12787268T DK2771460T3 DK 2771460 T3 DK2771460 T3 DK 2771460T3 DK 12787268 T DK12787268 T DK 12787268T DK 2771460 T3 DK2771460 T3 DK 2771460T3
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Denmark
Prior art keywords
atom
polypeptide
isoprene
cell
host cell
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DK12787268.7T
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Danish (da)
English (en)
Inventor
Christopher L Rife
Derek H Wells
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Danisco Us Inc
Goodyear Tire & Rubber
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Publication date
Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
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Publication of DK2771460T3 publication Critical patent/DK2771460T3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/03Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03007Pentalenene synthase (4.2.3.7)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L7/00Compositions of natural rubber

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK12787268.7T 2011-10-27 2012-10-26 ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE DK2771460T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161552453P 2011-10-27 2011-10-27
PCT/US2012/062308 WO2013063528A2 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Publications (1)

Publication Number Publication Date
DK2771460T3 true DK2771460T3 (en) 2017-06-06

Family

ID=47178953

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12787268.7T DK2771460T3 (en) 2011-10-27 2012-10-26 ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE

Country Status (8)

Country Link
US (2) US8735134B2 (enExample)
EP (1) EP2771460B1 (enExample)
JP (1) JP6101275B2 (enExample)
BR (1) BR112014010053A8 (enExample)
CA (1) CA2853125A1 (enExample)
DK (1) DK2771460T3 (enExample)
HK (1) HK1201554A1 (enExample)
WO (1) WO2013063528A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100184178A1 (en) * 2008-09-15 2010-07-22 Zachary Quinn Beck Increased isoprene production using mevalonate kinase and isoprene synthase
WO2013063528A2 (en) 2011-10-27 2013-05-02 Danisco Us Inc. Isoprene synthase variants with improved solubility for production of isoprene
US20140128645A1 (en) * 2011-12-23 2014-05-08 The Goodyear Tire & Rubber Company Enhanced production of isoprene using marine bacterial cells
US9783828B2 (en) 2012-10-23 2017-10-10 Sekisui Chemical Co., Ltd. Recombinant cell, and method for producing isoprene
JPWO2015076392A1 (ja) 2013-11-22 2017-03-16 味の素株式会社 改変イソプレンシンターゼ
CN105985971B (zh) * 2015-02-10 2021-01-22 中国科学院微生物研究所 异戊二烯合成酶基因及其应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8407828D0 (en) 1984-03-27 1984-05-02 Ontario Research Foundation In situ preparation
US4570029A (en) 1985-03-04 1986-02-11 Uop Inc. Process for separating isoprene
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5849970A (en) 1995-06-23 1998-12-15 The Regents Of The University Of Colorado Materials and methods for the bacterial production of isoprene
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
AU2001278098A1 (en) 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
US7371558B2 (en) 2002-10-04 2008-05-13 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Adeka 新規なアセトアセチルCoA合成酵素、それをコードするDNA配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
US20090076743A1 (en) 2007-08-01 2009-03-19 Drexel University Novel method for zero point detection
DK2235190T3 (en) 2007-12-13 2018-07-23 Danisco Us Inc COMPOSITIONS AND PROCEDURES FOR PRODUCING ISOPRENE
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
CA2729302A1 (en) 2008-06-30 2010-01-14 The Goodyear Tire & Rubber Company Polymers of isoprene from renewable resources
SG167566A1 (en) 2008-07-02 2011-01-28 Danisco Us Inc Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges
US20100184178A1 (en) 2008-09-15 2010-07-22 Zachary Quinn Beck Increased isoprene production using mevalonate kinase and isoprene synthase
US8476049B2 (en) 2008-09-15 2013-07-02 Danisco Us Inc. Conversion of prenyl derivatives to isoprene
WO2010031068A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
MY169163A (en) 2008-09-15 2019-02-19 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
WO2010031079A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
EP2382312A2 (en) 2008-12-30 2011-11-02 Danisco US Inc. Methods of producing isoprene and a co-product
US8518686B2 (en) 2009-04-23 2013-08-27 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201120213A (en) 2009-06-17 2011-06-16 Danisco Us Inc Polymerization of isoprene from renewable resources
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用dxp及mva途徑之改良之異戊二烯製造
CA2783976A1 (en) 2009-12-18 2011-06-23 Danisco Us Inc. Purification of isoprene from renewable resources
WO2011075748A1 (en) 2009-12-22 2011-06-23 Danisco Us Inc. Membrane bioreactor for increased production of isoprene gas
SG181856A1 (en) 2009-12-23 2012-07-30 Danisco Us Inc Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl)
CN103443271A (zh) 2010-10-27 2013-12-11 丹尼斯科美国公司 用于增加异戊二烯产量的异戊二烯合酶变体
WO2013063528A2 (en) 2011-10-27 2013-05-02 Danisco Us Inc. Isoprene synthase variants with improved solubility for production of isoprene

Also Published As

Publication number Publication date
BR112014010053A8 (pt) 2017-06-20
US20130295632A1 (en) 2013-11-07
JP2015503904A (ja) 2015-02-05
EP2771460B1 (en) 2017-04-26
EP2771460A2 (en) 2014-09-03
HK1201554A1 (en) 2015-09-04
US9273299B2 (en) 2016-03-01
US20140315275A1 (en) 2014-10-23
JP6101275B2 (ja) 2017-03-22
CA2853125A1 (en) 2013-05-02
WO2013063528A3 (en) 2013-06-20
WO2013063528A2 (en) 2013-05-02
US8735134B2 (en) 2014-05-27
BR112014010053A2 (pt) 2017-06-13

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