CA2853125A1 - Isoprene synthase variants with improved solubility for production of isoprene - Google Patents

Isoprene synthase variants with improved solubility for production of isoprene Download PDF

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Publication number
CA2853125A1
CA2853125A1 CA2853125A CA2853125A CA2853125A1 CA 2853125 A1 CA2853125 A1 CA 2853125A1 CA 2853125 A CA2853125 A CA 2853125A CA 2853125 A CA2853125 A CA 2853125A CA 2853125 A1 CA2853125 A1 CA 2853125A1
Authority
CA
Canada
Prior art keywords
atom
polypeptide
isoprene
cell
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2853125A
Other languages
English (en)
French (fr)
Inventor
Christopher L. Rife
Derek H. Wells
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Goodyear Tire and Rubber Co
Danisco US Inc
Original Assignee
Goodyear Tire and Rubber Co
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Goodyear Tire and Rubber Co, Danisco US Inc filed Critical Goodyear Tire and Rubber Co
Publication of CA2853125A1 publication Critical patent/CA2853125A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03007—Pentalenene synthase (4.2.3.7)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L7/00—Compositions of natural rubber

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2853125A 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene Abandoned CA2853125A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552453P 2011-10-27 2011-10-27
US61/552,453 2011-10-27
PCT/US2012/062308 WO2013063528A2 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Publications (1)

Publication Number Publication Date
CA2853125A1 true CA2853125A1 (en) 2013-05-02

Family

ID=47178953

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2853125A Abandoned CA2853125A1 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Country Status (8)

Country Link
US (2) US8735134B2 (enExample)
EP (1) EP2771460B1 (enExample)
JP (1) JP6101275B2 (enExample)
BR (1) BR112014010053A8 (enExample)
CA (1) CA2853125A1 (enExample)
DK (1) DK2771460T3 (enExample)
HK (1) HK1201554A1 (enExample)
WO (1) WO2013063528A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2737082A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
WO2013063528A2 (en) 2011-10-27 2013-05-02 Danisco Us Inc. Isoprene synthase variants with improved solubility for production of isoprene
EP2794851A2 (en) * 2011-12-23 2014-10-29 Danisco US Inc. Enhanced production of isoprene using marine bacterial cells
CN111621453A (zh) * 2012-10-23 2020-09-04 积水化学工业株式会社 重组细胞以及异戊二烯的生产方法
MY172689A (en) 2013-11-22 2019-12-10 Ajinomoto Kk Modified isoprene synthase
CN105985971B (zh) * 2015-02-10 2021-01-22 中国科学院微生物研究所 异戊二烯合成酶基因及其应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8407828D0 (en) 1984-03-27 1984-05-02 Ontario Research Foundation In situ preparation
US4570029A (en) 1985-03-04 1986-02-11 Uop Inc. Process for separating isoprene
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5849970A (en) 1995-06-23 1998-12-15 The Regents Of The University Of Colorado Materials and methods for the bacterial production of isoprene
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
US20030033626A1 (en) 2000-07-31 2003-02-13 Hahn Frederick M. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
US20090076743A1 (en) 2007-08-01 2009-03-19 Drexel University Novel method for zero point detection
BRPI0823506A8 (pt) 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
JP2011518564A (ja) * 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
SG167485A1 (en) 2008-06-30 2011-01-28 Danisco Us Inc Polymers of isoprene from renewable resources
EP2310490B1 (en) 2008-07-02 2019-01-02 Danisco US Inc. Methods for producing isoprene
CA2737082A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
CA2737223A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
MY156562A (en) 2008-09-15 2016-02-26 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
BRPI0918936A2 (pt) 2008-09-15 2019-09-24 Danisco Us Inc conversão de derivados de prenila em isopreno
WO2010031079A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
CN102333866B (zh) 2008-12-30 2015-04-29 丹尼斯科美国公司 生产异戊二烯和共同产物的方法
EP2421966A2 (en) 2009-04-23 2012-02-29 Danisco US Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TW201120213A (en) 2009-06-17 2011-06-16 Danisco Us Inc Polymerization of isoprene from renewable resources
CN102884028B (zh) 2009-12-18 2015-08-19 丹尼斯科美国公司 从可再生资源中纯化异戊二烯
CA2785530A1 (en) 2009-12-22 2011-06-23 Danisco Us Inc. Membrane bioreactor for increased production of isoprene gas
AU2010336342B2 (en) 2009-12-23 2015-02-26 Danisco Us Inc. Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL)
JP2014502148A (ja) * 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体
WO2013063528A2 (en) 2011-10-27 2013-05-02 Danisco Us Inc. Isoprene synthase variants with improved solubility for production of isoprene

Also Published As

Publication number Publication date
BR112014010053A2 (pt) 2017-06-13
JP6101275B2 (ja) 2017-03-22
WO2013063528A2 (en) 2013-05-02
US20140315275A1 (en) 2014-10-23
JP2015503904A (ja) 2015-02-05
EP2771460B1 (en) 2017-04-26
DK2771460T3 (en) 2017-06-06
WO2013063528A3 (en) 2013-06-20
US9273299B2 (en) 2016-03-01
HK1201554A1 (en) 2015-09-04
US8735134B2 (en) 2014-05-27
EP2771460A2 (en) 2014-09-03
BR112014010053A8 (pt) 2017-06-20
US20130295632A1 (en) 2013-11-07

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Effective date: 20181026