DK2640842T3 - Fremgangsmåder og sammensætninger til inducering af en immunreaktion på EGFRVIII - Google Patents
Fremgangsmåder og sammensætninger til inducering af en immunreaktion på EGFRVIII Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0011—Cancer antigens
- A61K39/001102—Receptors, cell surface antigens or cell surface determinants
- A61K39/001103—Receptors for growth factors
- A61K39/001104—Epidermal growth factor receptors [EGFR]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
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- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (16)
1. Immunogent polypeptid, hvis aminosyresekvens omfatter en flerhed af EG-FRvlll-polypeptidsekvenser, hvoraf hver sekvens omfatter EEKKGNYV (SEQ ID NO: 3), hvor hver EGFRvlll-polypeptidsekvens er flankeret af en sekvens, som er udformet til at blive spaltet af proteasomet.
2. Polypeptid ifølge krav 1, hvor flerheden af EGFRvlll-polypeptidsekvenser omfatter en eller flere aminosyresekvenser udvalgt fra gruppen bestående af LEEKKGNYV (SEQ ID NO: 4), LEEKKGNYWTDH (SEQ ID NO: 2) og PASRALEEKKGNYVVTDHGSC (SEQ ID NO: 5).
3. Polypeptid ifølge krav 1 eller 2, hvor flerheden af EGFRvlll-polypeptidsekvenser omfatter mindst tre kopier af PASRALEEKKGNYVVTDHGSC (SEQ ID NO: 5) eller mindst fem kopier af PASRALEEKKGNYVVTDHGSC (SEQ ID NO: 5).
4. Polypeptid ifølge et hvilket som helst af kravene 1 til 3, yderligere omfattende en del, der er udformet til at målrette polypeptidet mod en celleoverfladereceptor af en antigenpræsenterende celle.
5. Isoleret nukleinsyremolekyle, der koder for polypeptidet ifølge et hvilket som helst af kravene 1 til 4.
6. Isoleret nukleinsyremolekyle ifølge krav 5, hvor nukleinsyremolekylet er ko-don-optimeret til eksprimering gennem Listeria monocytogenes.
7. Isoleret nukleinsyremolekyle ifølge krav 5 eller 6, hvor nukleinsyremolekylet koder for det immunogene polypeptid som et fusionsprotein omfattende en sekretorisk signalsekvens.
8. Isoleret nukleinsyremolekyle ifølge krav 7, hvor den sekretoriske signalsekvens er en Listeria monocytogenes-ActA-signalsekvens eller en in frame-ActA-N100-sekvens udvalgt fra gruppen bestående af SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30 og SEQ ID NO: 31, eller en aminosyresekvens med mindst 90 % sekvensidentitet med ActA-N100-sekvensen.
9. Sammensætning omfattende en bakterie eller virus, som omfatter nukleinsyren ifølge et hvilket som helst af kravene 5 til 8.
10. Sammensætning ifølge krav 9, hvor bakterien er en eventuelt svækket eller dræbt, men metabolisk aktiv (KBMA) Listeria monocytogenes-bakterie omfattende nukleinsyren integreret i genomet af bakterien.
11. Sammensætning ifølge krav 10, hvor nukleinsyren er integreret i et virulensgen af bakterien, og integrationen af nukleinsyresekvensen forstyrrer eks-primeringen af virulensgenet eller forstyrrer en kodningssekvens af virulensgenet, eventuelt hvor virulensgenet er actA eller inIB.
12. Sammensætning ifølge krav 11, hvor bakterien er LmAactA/AinlB.
13. Sammensætning ifølge et hvilket som helst af kravene 9 til 12, hvor bakterien yderligere omfatter en genetisk mutation, der svækker bakteriens evne til at reparere nukleinsyre, eventuelt hvor den genetiske mutation er et eller flere gener udvalgt blandt phrB, uvrA, uvrB, uvrC, uvrD og recA.
14. Farmaceutisk sammensætning omfattende sammensætningen ifølge et hvilket som helst af kravene 9 til 13 eller nukleinsyren ifølge et hvilket som helst af kravene 5 til 8.
15. Farmaceutisk sammensætning ifølge krav 14 til anvendelse i en fremgangsmåde til inducering af et T-celle-respons mod EGFRvlll hos et individ, hvilken fremgangsmåde omfatter at eksprimere nukleinsyren ifølge et hvilket som helst af kravene 5 til 8 i individet under betingelser, der er udvalgt til at inducere T-celle-responset hos individet.
16. Farmaceutisk sammensætning til anvendelse ifølge krav 15, hvor individet har en EGFRvlll-eksprimerende malignitet, såsom et gliom.
Applications Claiming Priority (2)
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| US41485010P | 2010-11-17 | 2010-11-17 | |
| PCT/US2011/061164 WO2012068360A1 (en) | 2010-11-17 | 2011-11-17 | Methods and compositions for inducing an immune response to egfrviii |
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| DK2640842T3 true DK2640842T3 (da) | 2018-08-13 |
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| CN (1) | CN103415620B (da) |
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| SI (1) | SI2640842T1 (da) |
| WO (1) | WO2012068360A1 (da) |
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| WO2012068360A1 (en) * | 2010-11-17 | 2012-05-24 | Aduro Biotech | Methods and compositions for inducing an immune response to egfrviii |
| CA2831613A1 (en) | 2011-03-31 | 2012-10-04 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
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| DE12858350T1 (de) | 2011-12-16 | 2021-10-07 | Modernatx, Inc. | Modifizierte mrna zusammensetzungen |
| EP3501550A1 (en) | 2012-04-02 | 2019-06-26 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins associated with human disease |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US10501512B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides |
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| EP2640842A4 (en) | 2014-04-09 |
| CY1120622T1 (el) | 2019-12-11 |
| CA2818353A1 (en) | 2012-05-24 |
| US9200057B2 (en) | 2015-12-01 |
| ES2684684T3 (es) | 2018-10-04 |
| US20160074491A1 (en) | 2016-03-17 |
| US20180021420A1 (en) | 2018-01-25 |
| HRP20181343T1 (hr) | 2018-10-19 |
| JP5998370B2 (ja) | 2016-09-28 |
| HUE039747T2 (hu) | 2019-02-28 |
| PT2640842T (pt) | 2018-10-12 |
| EP2640842B1 (en) | 2018-05-30 |
| LT2640842T (lt) | 2018-09-10 |
| CN103415620A (zh) | 2013-11-27 |
| PL2640842T3 (pl) | 2018-11-30 |
| CN103415620B (zh) | 2016-10-12 |
| US20140037662A1 (en) | 2014-02-06 |
| SI2640842T1 (sl) | 2018-09-28 |
| EP2640842A1 (en) | 2013-09-25 |
| US9775891B2 (en) | 2017-10-03 |
| WO2012068360A1 (en) | 2012-05-24 |
| JP2014504851A (ja) | 2014-02-27 |
| RS57630B1 (sr) | 2018-11-30 |
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