DK2553090T3 - Termostabile phytasevarianter - Google Patents

Termostabile phytasevarianter

Info

Publication number
DK2553090T3
DK2553090T3 DK11709966.3T DK11709966T DK2553090T3 DK 2553090 T3 DK2553090 T3 DK 2553090T3 DK 11709966 T DK11709966 T DK 11709966T DK 2553090 T3 DK2553090 T3 DK 2553090T3
Authority
DK
Denmark
Prior art keywords
phytase variants
thermostable phytase
thermostable
variants
phytase
Prior art date
Application number
DK11709966.3T
Other languages
English (en)
Inventor
Lars Kobberoee Skov
Maria Leonardo De
Tomoko Matsui
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2553090T3 publication Critical patent/DK2553090T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/195Proteins from microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030083-Phytase (3.1.3.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030264-Phytase (3.1.3.26), i.e. 6-phytase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030725-Phytase (3.1.3.72)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK11709966.3T 2010-03-26 2011-03-25 Termostabile phytasevarianter DK2553090T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10158027 2010-03-26
PCT/EP2011/054639 WO2011117396A2 (en) 2010-03-26 2011-03-25 Thermostable phytase variants

Publications (1)

Publication Number Publication Date
DK2553090T3 true DK2553090T3 (da) 2019-05-13

Family

ID=44123229

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11709966.3T DK2553090T3 (da) 2010-03-26 2011-03-25 Termostabile phytasevarianter

Country Status (10)

Country Link
US (2) US20130195829A1 (da)
EP (1) EP2553090B1 (da)
CN (1) CN102906254A (da)
AU (1) AU2011231562B2 (da)
BR (1) BR112012023840B1 (da)
CA (2) CA2794095C (da)
DK (1) DK2553090T3 (da)
ES (1) ES2723050T3 (da)
PL (1) PL2553090T3 (da)
WO (1) WO2011117396A2 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2617816B1 (en) 2006-09-21 2015-11-18 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
AR084894A1 (es) 2011-02-18 2013-07-10 Danisco Composicion de aditivo para pienso
GB201102857D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
GB201102865D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
PL2729809T3 (pl) 2011-07-07 2019-04-30 Dupont Nutrition Biosci Aps Test oznaczający
GB201213801D0 (en) 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) * 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
EP2799531A1 (de) 2013-05-03 2014-11-05 Clariant Produkte (Deutschland) GmbH Einsatz von Phosphatasen zur enzymatischen Entschleimung von Triglyceriden
EP3027762A4 (en) 2013-07-25 2017-06-28 BASF Enzymes LLC Phytase
EP3270162B2 (en) 2013-08-09 2023-08-16 BioMadison, Inc. Botulinum toxin assay with improved sensitivity
CN104450643B (zh) * 2014-12-19 2020-07-28 青岛蔚蓝生物集团有限公司 植酸酶突变体及其应用
TWI591180B (zh) 2016-02-18 2017-07-11 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI616532B (zh) * 2016-02-18 2018-03-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI607088B (zh) * 2016-02-18 2017-12-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI615471B (zh) * 2016-02-18 2018-02-21 基酵生物科技股份有限公司 提升耐熱性的植酸酶
CN110129298B (zh) * 2016-06-02 2021-08-03 天津科技大学 一种磷脂酶d突变体及其应用
CN111867388A (zh) 2018-02-08 2020-10-30 丹尼斯科美国公司 用于酶包封的耐热蜡基质颗粒
CN112367847A (zh) 2018-04-26 2021-02-12 丹尼斯科美国公司 增加固体组合物中植酸酶的稳定性的方法及包含磷酸盐和植酸酶的颗粒组合物
CN112204136B (zh) * 2018-05-30 2024-05-14 南京百斯杰生物工程有限公司 植酸酶突变体
AU2020310162A1 (en) 2019-07-09 2022-02-03 International N&H Denmark Aps Fat coated particulate enzyme compositions
EP4013236A1 (en) 2019-08-16 2022-06-22 DuPont Nutrition Biosciences ApS Compositions for gut health comprising combinations of lactobacillus strains
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
CN114828642A (zh) 2019-10-21 2022-07-29 杜邦营养生物科学有限公司 用于消化道健康的组合物
EP4099838A1 (en) 2020-02-07 2022-12-14 DuPont Nutrition Biosciences ApS Feed compositions for animal health
CA3189083A1 (en) 2020-08-13 2022-02-17 Novozymes A/S Phytase variants and polynucleotides encoding same
CN117241678A (zh) 2020-10-16 2023-12-15 杜邦营养生物科学有限公司 用于动物健康的饲料组合物
WO2022169933A2 (en) 2021-02-03 2022-08-11 Dupont Nutrition Biosciences Aps Compositions for gut health
KR20240060703A (ko) 2021-09-27 2024-05-08 인터내셔널 엔&에이치 덴마크 에이피에스 사료 첨가제 조성물 및 이의 사용 방법

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
WO2004085638A1 (en) 2003-03-25 2004-10-07 Republic Of National Fisheries Research And Development Institute Phytase produced from citrobacter braakii
AR050895A1 (es) * 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
EP1809747B1 (en) * 2004-10-04 2016-12-14 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
WO2006038128A2 (en) 2004-10-04 2006-04-13 Danisco A/S Citrobacter freundii phytase and homologues
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
WO2007112739A1 (en) * 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
US8101391B2 (en) * 2007-01-30 2012-01-24 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
CN101679986B (zh) * 2007-03-26 2016-08-10 诺维信公司 哈夫尼菌属肌醇六磷酸酶
CA2704518C (en) * 2007-12-03 2018-02-20 Syngenta Participations Ag Engineering enzymatically susceptible proteins
EP2933329B1 (en) * 2008-09-26 2017-05-17 Novozymes A/S Hafnia phytase variants
FR2947563B1 (fr) * 2009-07-01 2015-08-21 Biomethodes Variants ameliores d'une phytase
US8334124B1 (en) * 2009-09-23 2012-12-18 The United States Of America As Represented By The Secretary Of Agriculture Modified Aspergillus niger phytase
BR112012009483A2 (pt) * 2009-10-22 2015-09-15 Basf Se fitase, sequência de ácido nucleico isolada, vetor de expressão recombinante, célula hospedeira recombinante, organismo de produção recombinante, aditivo de ração animal, ração animal, e, uso de uma fitase
ES2594359T3 (es) * 2010-03-26 2016-12-19 Novozymes A/S Variantes de fitasa termoestables
ES2737885T3 (es) * 2010-03-26 2020-01-16 Novozymes As Variantes termoestables de fitasa
AR084894A1 (es) * 2011-02-18 2013-07-10 Danisco Composicion de aditivo para pienso
WO2012143862A1 (en) * 2011-04-21 2012-10-26 Basf Se Synthetic phytase variants

Also Published As

Publication number Publication date
CN102906254A (zh) 2013-01-30
EP2553090A2 (en) 2013-02-06
AU2011231562B2 (en) 2015-10-22
ES2723050T3 (es) 2019-08-21
EP2553090B1 (en) 2019-02-27
CA2794095A1 (en) 2011-09-29
WO2011117396A2 (en) 2011-09-29
CA3105079A1 (en) 2011-09-29
BR112012023840A2 (pt) 2020-07-28
CA2794095C (en) 2021-02-16
US9611469B2 (en) 2017-04-04
BR112012023840B1 (pt) 2021-06-22
US20130195829A1 (en) 2013-08-01
PL2553090T3 (pl) 2019-08-30
WO2011117396A3 (en) 2011-11-24
US20150159149A1 (en) 2015-06-11
AU2011231562A1 (en) 2012-09-27

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