DK2467489T3 - Baculovirus-baseret produktion af biofarmaceutiske midler frie for forurenende baculovirale virioner - Google Patents
Baculovirus-baseret produktion af biofarmaceutiske midler frie for forurenende baculovirale virioner Download PDFInfo
- Publication number
- DK2467489T3 DK2467489T3 DK10743083.7T DK10743083T DK2467489T3 DK 2467489 T3 DK2467489 T3 DK 2467489T3 DK 10743083 T DK10743083 T DK 10743083T DK 2467489 T3 DK2467489 T3 DK 2467489T3
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- DK
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- Prior art keywords
- baculovirus
- gene
- cells
- bacmid
- egfp
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14151—Methods of production or purification of viral material
- C12N2710/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/50—Vectors for producing vectors
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (9)
1. Fremgangsmåde til fremstillingen af et biofarmaceutisk produkt, omfattende: (a) inficere en biofarmaceutisk-producerende insektcelle med mindst ét baculovirus, idet det mindst ene baculovirus omfattende et genom der koder for det biofarmaceutiske produkt, og (b) opretholde den biofarmaceutisk-producerende insektcelle under betingelser så at det biofarmaceutiske produkt fremstilles, hvor genomet af det mindst ene baculovirus er mangelfuldt for vp80 eller hvor den biofarmaceutisk-producerende insektcelle omfatter et ekspressionskontrolsystem der tillader aktiveringen af vp80.
2. Fremgangsmåden ifølge krav 1, hvor - vp80 er gjort mangelfuldt i genomet ved hjælp af nukleotidsubstitution, insertion eller deletion; eller - hvor den biofarmaceutisk-producerende insektcelle er en rekombinant insektcelle omfattende et konstrukt der udtrykker dsRNA der er specifik for vp80, dsRNA'et udtrykkes eventuelt under en inducerbar promotor.
3. Fremgangsmåden ifølge krav 1 eller 2, hvor det mindst ene baculovirus fremstilles før trin (a) i en baculovirus-producerende celle der udtrykker en komplementerende kopi af vp80.
4. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 3, hvor mangelfuldheden eller inaktivering af vp80 ikke påvirker meget sen ekspression fra baculovirus i sammenligning med meget sen ekspression fra vild-type baculovirus.
5. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 4, hvor det biofarmaceutiske produkt er et rekombinant protein, et rekombinant virus eller en virus-lignende partikel.
6. Fremgangsmåden ifølge krav 5, hvor the biofarmaceutisk produkt er et rekombinant AAV.
7. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 6, hvor det biofarmaceutiske produkt kodes af mindst ét gen indført i det rekombinante baculovirusgenom under kontrollen af polyhedrin eller plO promotor.
8. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7, hvor det mindst ene baculovirus er afledt fra AcMNPV eller BmNPV.
9. Anvendelse af et baculovirus-insektcellesystem til fremstillingen af et biofarmaceutisk produkt hvor baculovirus-insektcellesystemet omfatter en baculovirus-producerende insektcelle inficeret med mindst ét rekombinant baculovirus, hvor: - den, eller hvert, rekombinante baculovirus omfatter et baculovirusgenom der koder for det biofarmaceutiske produkt, eller mindst én komponent af det biofarmaceutiske produkt, og - den, eller de, rekombinante baculovirale genom er mangelfuldt for vp80, eller den baculovirus-producerende insektcelle omfatter et ekspressionskontrolsystem der tillader aktiveringen af vp80.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09305761A EP2292781A1 (en) | 2009-08-17 | 2009-08-17 | Baculovirus-based production of biopharmaceuticals free of contaminating baculoviral virions |
| PCT/EP2010/061456 WO2011020710A2 (en) | 2009-08-17 | 2010-08-05 | Baculovirus-based production of biopharmaceuticals free of contaminating baculoviral virions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2467489T3 true DK2467489T3 (da) | 2015-07-20 |
Family
ID=41203903
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16205440.7T DK3187589T3 (da) | 2009-08-17 | 2010-08-05 | Baculovirus-baseret produktion af biofarmaceutiske midler fri for forurenende baculovirale virioner |
| DK15168775.3T DK2933336T3 (da) | 2009-08-17 | 2010-08-05 | Baculovirus-baseret produktion af biofarmaceutika frie for forurenende baculovirale virioner |
| DK10743083.7T DK2467489T3 (da) | 2009-08-17 | 2010-08-05 | Baculovirus-baseret produktion af biofarmaceutiske midler frie for forurenende baculovirale virioner |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16205440.7T DK3187589T3 (da) | 2009-08-17 | 2010-08-05 | Baculovirus-baseret produktion af biofarmaceutiske midler fri for forurenende baculovirale virioner |
| DK15168775.3T DK2933336T3 (da) | 2009-08-17 | 2010-08-05 | Baculovirus-baseret produktion af biofarmaceutika frie for forurenende baculovirale virioner |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US8993317B2 (da) |
| EP (4) | EP2292781A1 (da) |
| CN (1) | CN102686732A (da) |
| CA (2) | CA3050271A1 (da) |
| DK (3) | DK3187589T3 (da) |
| ES (3) | ES2787706T3 (da) |
| HU (1) | HUE025056T2 (da) |
| PL (2) | PL2467489T3 (da) |
| PT (3) | PT2933336T (da) |
| WO (1) | WO2011020710A2 (da) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2292781A1 (en) | 2009-08-17 | 2011-03-09 | Genethon | Baculovirus-based production of biopharmaceuticals free of contaminating baculoviral virions |
| FR2978456B1 (fr) * | 2011-07-27 | 2015-02-20 | Genethon | Systeme baculoviral pour l'expression d'un vecteur de therapie genique |
| US20140287460A1 (en) * | 2011-07-27 | 2014-09-25 | Gbiotech Sarl | Production of recombinant protein in insect cells using a baculovirus expression system |
| TWI702955B (zh) | 2012-05-15 | 2020-09-01 | 澳大利亞商艾佛蘭屈澳洲私營有限公司 | 使用腺相關病毒（ａａｖ）ｓｆｌｔ-１治療老年性黃斑部退化（ａｍｄ） |
| US9982239B2 (en) | 2012-06-12 | 2018-05-29 | Alternative Gene Expression S.L. | Baculoviral DNA elements for the expression of recombinant proteins in a host cell |
| EP3119798B1 (en) | 2014-03-17 | 2020-08-05 | Adverum Biotechnologies, Inc. | Compositions and methods for enhanced gene expression in cone cells |
| EP3151866B1 (en) | 2014-06-09 | 2023-03-08 | Voyager Therapeutics, Inc. | Chimeric capsids |
| SG11201703148TA (en) | 2014-11-05 | 2017-05-30 | Voyager Therapeutics Inc | Aadc polynucleotides for the treatment of parkinson's disease |
| IL284949B2 (en) | 2014-11-14 | 2024-06-01 | Voyager Therapeutics Inc | modulatory polynucleotides |
| US10597660B2 (en) | 2014-11-14 | 2020-03-24 | Voyager Therapeutics, Inc. | Compositions and methods of treating amyotrophic lateral sclerosis (ALS) |
| EP3230441A4 (en) | 2014-12-12 | 2018-10-03 | Voyager Therapeutics, Inc. | Compositions and methods for the production of scaav |
| AU2016226289B2 (en) | 2015-03-02 | 2021-04-29 | Adverum Biotechnologies, Inc. | Compositions and methods for intravitreal delivery of polynucleotides to retinal cones |
| IL292830B2 (en) | 2015-09-28 | 2023-06-01 | Univ Florida | Methods and compositions for stealth virus antibody vectors |
| GB2545763A (en) | 2015-12-23 | 2017-06-28 | Adverum Biotechnologies Inc | Mutant viral capsid libraries and related systems and methods |
| WO2017189959A1 (en) | 2016-04-29 | 2017-11-02 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| US11299751B2 (en) | 2016-04-29 | 2022-04-12 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| CN109831916B (zh) | 2016-05-18 | 2023-07-21 | 沃雅戈治疗公司 | 治疗亨廷顿氏舞蹈病的组合物和方法 |
| KR102392236B1 (ko) | 2016-05-18 | 2022-05-03 | 보이저 테라퓨틱스, 인크. | 조절성 폴리뉴클레오티드 |
| EP3464596A1 (en) * | 2016-06-07 | 2019-04-10 | Cepheid | Thermostable polymerase inhibitor compositions and methods |
| EP3831281A1 (en) | 2016-08-30 | 2021-06-09 | The Regents of The University of California | Methods for biomedical targeting and delivery and devices and systems for practicing the same |
| EP3618839A4 (en) | 2017-05-05 | 2021-06-09 | Voyager Therapeutics, Inc. | COMPOSITIONS AND TREATMENT METHODS FOR AMYOTROPHIC LATERAL SCLEROSIS (ALS) |
| MX2019013172A (es) | 2017-05-05 | 2020-09-07 | Voyager Therapeutics Inc | Composiciones y metodos para tratar la enfermedad de huntington. |
| JOP20190269A1 (ar) | 2017-06-15 | 2019-11-20 | Voyager Therapeutics Inc | بولي نوكليوتيدات aadc لعلاج مرض باركنسون |
| CN111132626B (zh) | 2017-07-17 | 2024-01-30 | 沃雅戈治疗公司 | 轨迹阵列引导系统 |
| US11512327B2 (en) | 2017-08-03 | 2022-11-29 | Voyager Therapeutics, Inc. | Compositions and methods for delivery of AAV |
| EP3697908A1 (en) | 2017-10-16 | 2020-08-26 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (als) |
| WO2019079240A1 (en) | 2017-10-16 | 2019-04-25 | Voyager Therapeutics, Inc. | TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS (ALS) |
| KR20210006358A (ko) | 2018-04-03 | 2021-01-18 | 스트라이드바이오 인코포레이티드 | 안 조직을 표적으로 하기 위한 바이러스 벡터 |
| AU2019247748A1 (en) | 2018-04-03 | 2020-10-08 | Ginkgo Bioworks, Inc. | Antibody-evading virus vectors |
| CA3095179A1 (en) | 2018-04-03 | 2019-10-10 | Stridebio, Inc. | Antibody-evading virus vectors |
| WO2019237374A1 (zh) * | 2018-06-16 | 2019-12-19 | 深圳市博奥康生物科技有限公司 | Mtabc3基因定点整合至a375细胞的方法及其应用 |
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| CN108998455B (zh) * | 2018-08-13 | 2021-03-16 | 西南大学 | 家蚕核型多角体病毒诱导型39k启动子及其重组载体和应用 |
| CN111434774B (zh) * | 2019-01-11 | 2023-06-23 | 陕西杆粒生物科技有限公司 | 一种解除高滴度抑制的杆状病毒表达载体 |
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| CN114286688A (zh) * | 2019-06-26 | 2022-04-05 | 威洛克有限公司 | 杆状病毒表达系统 |
| IL292297A (en) | 2019-10-17 | 2022-06-01 | Stridebio Inc | Adeno-associated viral vectors for the treatment of Niemann-Pick type c disease |
| CN110940807A (zh) * | 2019-11-08 | 2020-03-31 | 江苏科技大学 | 研究家蚕基因抑制BmNPV DNA复制相关基因的表达的方法 |
| EP4200408A1 (en) | 2020-08-19 | 2023-06-28 | Sarepta Therapeutics, Inc. | Adeno-associated virus vectors for treatment of rett syndrome |
| WO2022046665A1 (en) * | 2020-08-23 | 2022-03-03 | Bioverativ Therapeutics Inc. | MODIFIED BACULOVIRUS SYSTEM FOR IMPROVED PRODUCTION OF CLOSED-ENDED DNA (ceDNA) |
| CN115491377A (zh) * | 2021-06-18 | 2022-12-20 | 舒泰神（北京）生物制药股份有限公司 | 一种诱导rna干扰和降低并清除细胞中病毒污染的核苷酸序列及应用 |
| IL310879A (en) * | 2021-08-23 | 2024-04-01 | Bioverativ Therapeutics Inc | Expression system in colovirus |
| CN114058598B (zh) * | 2021-11-04 | 2023-04-28 | 中国科学院精密测量科学与技术创新研究院 | 新的重组杆状病毒基因组插入位点及其应用 |
| TW202404993A (zh) | 2022-04-11 | 2024-02-01 | 美商特納亞治療股份有限公司 | 具經工程化蛋白殼之腺相關病毒 |
| CN118086400B (zh) * | 2024-04-17 | 2024-07-19 | 和元生物技术（上海）股份有限公司 | 核酸分子、包含其的重组杆状病毒及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE58011B1 (en) | 1983-05-27 | 1993-06-16 | Texas A & M Univ Sys | Method for producing a recombinant baculovirus expression vector |
| US4745051A (en) | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
| ZA848495B (en) | 1984-01-31 | 1985-09-25 | Idaho Res Found | Production of polypeptides in insect cells |
| CN1062601C (zh) * | 1992-03-24 | 2001-02-28 | 剑桥药物研究有限公司 | 病毒疫苗 |
| FR2756297B1 (fr) * | 1996-11-22 | 1999-01-08 | Centre Nat Rech Scient | Procede de production de virus recombinants |
| GB9919409D0 (en) * | 1999-08-18 | 1999-10-20 | Univ Oxford Brookes | Baculovirus expression system |
| WO2004009768A2 (en) * | 2002-07-18 | 2004-01-29 | Invitrogen Corporation | Viral vectors containing recombination sites |
| US20060166363A1 (en) * | 2004-01-27 | 2006-07-27 | Sergei Zolotukhin | Modified baculovirus expression system for production of pseudotyped rAAV vector |
| EP2292781A1 (en) * | 2009-08-17 | 2011-03-09 | Genethon | Baculovirus-based production of biopharmaceuticals free of contaminating baculoviral virions |
-
2009
- 2009-08-17 EP EP09305761A patent/EP2292781A1/en not_active Withdrawn
-
2010
- 2010-08-05 DK DK16205440.7T patent/DK3187589T3/da active
- 2010-08-05 CA CA3050271A patent/CA3050271A1/en not_active Abandoned
- 2010-08-05 CN CN2010800456298A patent/CN102686732A/zh active Pending
- 2010-08-05 PL PL10743083T patent/PL2467489T3/pl unknown
- 2010-08-05 PT PT151687753T patent/PT2933336T/pt unknown
- 2010-08-05 WO PCT/EP2010/061456 patent/WO2011020710A2/en active Application Filing
- 2010-08-05 ES ES16205440T patent/ES2787706T3/es active Active
- 2010-08-05 ES ES10743083.7T patent/ES2542740T3/es active Active
- 2010-08-05 EP EP15168775.3A patent/EP2933336B1/en not_active Not-in-force
- 2010-08-05 PT PT107430837T patent/PT2467489E/pt unknown
- 2010-08-05 EP EP10743083.7A patent/EP2467489B1/en not_active Not-in-force
- 2010-08-05 EP EP16205440.7A patent/EP3187589B1/en active Active
- 2010-08-05 CA CA2771250A patent/CA2771250C/en not_active Expired - Fee Related
- 2010-08-05 DK DK15168775.3T patent/DK2933336T3/da active
- 2010-08-05 HU HUE10743083A patent/HUE025056T2/en unknown
- 2010-08-05 PT PT162054407T patent/PT3187589T/pt unknown
- 2010-08-05 US US13/390,806 patent/US8993317B2/en not_active Expired - Fee Related
- 2010-08-05 ES ES15168775.3T patent/ES2627744T3/es active Active
- 2010-08-05 DK DK10743083.7T patent/DK2467489T3/da active
- 2010-08-05 PL PL16205440T patent/PL3187589T3/pl unknown
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2015
- 2015-03-27 US US14/670,459 patent/US9862934B2/en active Active
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2017
- 2017-12-14 US US15/841,359 patent/US10428315B2/en not_active Expired - Fee Related
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2019
- 2019-08-27 US US16/551,787 patent/US11236307B2/en active Active
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