DK2414544T3 - Probe-amplifikations-afhængig kemisk ligering - Google Patents

Probe-amplifikations-afhængig kemisk ligering

Info

Publication number
DK2414544T3
DK2414544T3 DK10759145.5T DK10759145T DK2414544T3 DK 2414544 T3 DK2414544 T3 DK 2414544T3 DK 10759145 T DK10759145 T DK 10759145T DK 2414544 T3 DK2414544 T3 DK 2414544T3
Authority
DK
Denmark
Prior art keywords
chemical ligation
probe amplification
dependent chemical
dependent
amplification
Prior art date
Application number
DK10759145.5T
Other languages
English (en)
Inventor
Robert Terbrueggen
Original Assignee
Dxterity Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dxterity Diagnostics Inc filed Critical Dxterity Diagnostics Inc
Application granted granted Critical
Publication of DK2414544T3 publication Critical patent/DK2414544T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
DK10759145.5T 2009-04-01 2010-03-29 Probe-amplifikations-afhængig kemisk ligering DK2414544T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16583909P 2009-04-01 2009-04-01
PCT/US2010/000949 WO2010114599A1 (en) 2009-04-01 2010-03-29 Chemical ligation dependent probe amplification (clpa)

Publications (1)

Publication Number Publication Date
DK2414544T3 true DK2414544T3 (da) 2014-06-16

Family

ID=42828614

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10759145.5T DK2414544T3 (da) 2009-04-01 2010-03-29 Probe-amplifikations-afhængig kemisk ligering

Country Status (11)

Country Link
US (2) US9976177B2 (da)
EP (2) EP2765205B1 (da)
JP (1) JP2012531887A (da)
CN (1) CN102449169B (da)
AU (1) AU2010232972B2 (da)
CA (1) CA2757300C (da)
DK (1) DK2414544T3 (da)
ES (1) ES2469092T3 (da)
HK (2) HK1167269A1 (da)
SM (1) SMT201400108B (da)
WO (1) WO2010114599A1 (da)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2677039B8 (en) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
AU2010232972B2 (en) 2009-04-01 2016-10-27 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
US9175340B2 (en) * 2009-11-04 2015-11-03 President And Fellows Of Harvard College Reactivity-dependent and interaction-dependent PCR
CN105964312A (zh) 2010-07-22 2016-09-28 基因细胞生物系统有限公司 复合液体池
GB2497501A (en) 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
KR101322880B1 (ko) 2011-03-04 2013-10-29 한국과학기술원 Ｓｄｌ－ｐｃｒ을 이용한 유전자 분석방법
US20140170653A1 (en) * 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
KR102085242B1 (ko) 2011-05-17 2020-03-05 디엑스테리티 다이아그노스틱스 인코포레이티드 표적 핵산 검출을 위한 방법 및 조성물
US20140155275A1 (en) * 2011-07-06 2014-06-05 The Johns Hopkins University Semi-digital ligation assay
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9725761B2 (en) 2011-12-28 2017-08-08 Ricardo Mancebo Reagents and methods for autoligation chain reaction
WO2013106807A1 (en) 2012-01-13 2013-07-18 Curry John D Scalable characterization of nucleic acids by parallel sequencing
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
CN103958696B (zh) * 2012-09-10 2017-10-13 天昊生物医药科技（苏州）有限公司 多重核酸分析
US20140081665A1 (en) 2012-09-11 2014-03-20 Theranos, Inc. Information management systems and methods using a biological signature
JP6440627B2 (ja) 2012-11-27 2018-12-19 ジェンセル バイオシステムズ リミテッド 液体試料の処理
WO2014089536A1 (en) * 2012-12-07 2014-06-12 Invitae Corporation Multiplex nucleic acid detection methods
CN103898199B (zh) * 2012-12-27 2016-12-28 上海天昊生物科技有限公司 一种高通量核酸分析方法及其应用
US10053725B2 (en) 2013-04-23 2018-08-21 President And Fellows Of Harvard College In situ interaction determination
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
CN103266180A (zh) * 2013-06-07 2013-08-28 徐堤 通用型多重连接依赖探针扩增试剂盒
WO2015011200A1 (en) * 2013-07-23 2015-01-29 Universitat Autònoma De Barcelona INVERSE MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION (iMLPA), AN IN VITRO METHOD OF GENOTYPING MULTIPLE INVERSIONS
CN105723222B (zh) 2013-10-23 2019-01-22 吉尼亚科技公司 使用纳米孔的高速分子感测
CN103555846B (zh) * 2013-11-07 2015-12-30 中国海洋大学 一种基于液相分子杂交原理的高通量低成本snp分型方法
EP3105326A4 (en) * 2014-02-10 2018-01-10 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
WO2015191777A2 (en) 2014-06-10 2015-12-17 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
US20180023138A1 (en) * 2014-11-01 2018-01-25 Singular Bio, Inc. Assays for Single Molecule Detection and Use Thereof
CN104561274B (zh) * 2014-12-16 2017-06-30 中国科学院苏州生物医学工程技术研究所 一种检测待测液中microRNA含量的方法
CN105803055A (zh) * 2014-12-31 2016-07-27 天昊生物医药科技（苏州）有限公司 一种基于多重循环延伸连接的靶基因区域富集新方法
AU2016219943A1 (en) 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
US11634705B2 (en) 2016-03-30 2023-04-25 Epsilon Molecular Engineering Inc. High-speed in vitro screening method
WO2017170776A1 (ja) * 2016-03-30 2017-10-05 株式会社Ｅｐｓｉｌｏｎ Ｍｏｌｅｃｕｌａｒ Ｅｎｇｉｎｅｅｒｉｎｇ 高速試験管内スクリーニング法
DK3440205T3 (da) 2016-04-07 2021-08-16 Univ Leland Stanford Junior Ikke-invasiv diagnostik ved sekventering af 5-hydroxymethyleret cellefrit dna
CA3074461A1 (en) 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
US10900032B2 (en) 2019-05-07 2021-01-26 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
EP3990926A4 (en) * 2019-06-26 2023-09-13 Bio-Rad Laboratories, Inc. SYSTEM AND METHOD FOR RECOVERY OF TARGET MATERIAL FROM MICROWELLS
CN111621551B (zh) * 2019-07-31 2021-10-26 深圳闪量科技有限公司 多重连接探针微阵列检测
CN112410404B (zh) * 2020-05-27 2023-05-19 江西省肿瘤医院(江西省癌症中心) 一种开启型双光子核酸探针及其在fish中的应用
EP3988669A1 (en) * 2020-10-22 2022-04-27 Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft Method for nucleic acid detection by oligo hybridization and pcr-based amplification
TWI817200B (zh) * 2021-09-23 2023-10-01 中國文化大學 一種檢測標的dna序列基因型是否變異之方法

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JP2801051B2 (ja) 1988-06-24 1998-09-21 アムジエン・インコーポレーテツド 核酸塩基配列を検出するための方法及び試薬
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5602240A (en) * 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5683874A (en) * 1991-03-27 1997-11-04 Research Corporation Technologies, Inc. Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
ES2204913T3 (es) * 1993-04-12 2004-05-01 Northwestern University Metodo para formacion de oligonucleotidos.
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
CA2105364A1 (en) 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5808036A (en) * 1993-09-01 1998-09-15 Research Corporation Technologies Inc. Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US5674683A (en) * 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5780613A (en) * 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
EP1958955B1 (en) 1996-02-09 2013-09-04 Cornell Research Foundation, Inc. Spatially addressable oligonucleotide arrays and method of making the same
US6218108B1 (en) * 1997-05-16 2001-04-17 Research Corporation Technologies, Inc. Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
EP1736554B1 (en) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
EP0923595B1 (en) 1996-06-07 2005-11-02 Amersham Biosciences (SV) Corp. Nucleic acid mediated electron transfer
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU739375B2 (en) 1996-11-05 2001-10-11 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
JP2002500897A (ja) 1998-01-27 2002-01-15 クリニカル・マイクロ・センサーズ・インコーポレイテッド 電子的核酸検出の増幅
AU3671099A (en) 1998-04-29 1999-11-16 Trustees Of Boston University Methods and compositions pertaining to pd-loops
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6479650B1 (en) * 1999-12-14 2002-11-12 Research Corporation Technologies, Inc. Fluorescent nucleoside analogs and combinatorial fluorophore arrays comprising same
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
AU2001265121A1 (en) * 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
CA2415125A1 (en) * 2000-07-03 2002-01-10 Applera Corporation Polynucleotide sequence assay
DE60231606D1 (de) * 2001-02-27 2009-04-30 Virco Bvba Zirkuläre sonden amplifizierung (cpa) von zirkularisierten nucleinsäure-molekülen
WO2002083837A1 (en) * 2001-04-11 2002-10-24 Ptc Therapeutics, Inc. Methods for identifying small molecules that bind specific rna structural motifs
US20020197630A1 (en) * 2001-04-12 2002-12-26 Knapp Michael R. Systems and methods for high throughput genetic analysis
FI20021325A0 (fi) 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
US7749699B2 (en) 2002-07-18 2010-07-06 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
CA2499360A1 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
WO2004027081A2 (en) * 2002-09-19 2004-04-01 Applera Corporation Methods and composition for detecting targets
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US7459273B2 (en) * 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) * 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
KR100959101B1 (ko) * 2003-02-20 2010-05-25 삼성전자주식회사 Ｐｃｒ 반응기 및 ｐｃｒ 반응기의 입구와 출구의 개폐를조절하는 방법
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
EP1680517B1 (en) * 2003-11-04 2010-03-31 Applied Biosystems, LLC Concatameric ligation products: compositions, methods and kits for same
EP1702216B1 (en) * 2004-01-09 2015-11-04 Life Technologies Corporation Phosphor particle coded beads
US20050208503A1 (en) * 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
EP2161346B1 (en) * 2004-03-24 2013-06-19 Applied Biosystems, LLC Ligation and amplification reactions for determining target molecules
WO2006076017A2 (en) * 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7745614B2 (en) * 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
EP2677039B8 (en) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7899626B2 (en) * 2007-01-10 2011-03-01 Illumina, Inc. System and method of measuring methylation of nucleic acids
AU2010232972B2 (en) 2009-04-01 2016-10-27 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
CA2906920C (en) 2013-03-15 2021-01-05 Armour Technologies, Inc. Medical device curving apparatus, system, and method of use
FR3054894A1 (fr) 2016-08-03 2018-02-09 Stmicroelectronics (Crolles 2) Sas Dispositif integre photonique a compacite amelioree

Also Published As

Publication number Publication date
CA2757300A1 (en) 2010-10-07
HK1167269A1 (zh) 2012-11-23
SMT201400108B (it) 2014-09-08
US20100267585A1 (en) 2010-10-21
ES2469092T3 (es) 2014-06-17
EP2765205B1 (en) 2016-05-18
US20190106739A1 (en) 2019-04-11
CA2757300C (en) 2018-01-09
EP2414544A1 (en) 2012-02-08
HK1201081A1 (zh) 2015-08-21
EP2414544B1 (en) 2014-04-23
EP2414544A4 (en) 2012-09-05
CN102449169A (zh) 2012-05-09
AU2010232972B2 (en) 2016-10-27
WO2010114599A1 (en) 2010-10-07
EP2765205A1 (en) 2014-08-13
AU2010232972A1 (en) 2011-11-10
US9976177B2 (en) 2018-05-22
CN102449169B (zh) 2016-03-16
JP2012531887A (ja) 2012-12-13

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