DK2379717T3 - Fremgangsmåde til fremstilling af et enzymprodukt - Google Patents

Fremgangsmåde til fremstilling af et enzymprodukt Download PDF

Info

Publication number
DK2379717T3
DK2379717T3 DK09797043.8T DK09797043T DK2379717T3 DK 2379717 T3 DK2379717 T3 DK 2379717T3 DK 09797043 T DK09797043 T DK 09797043T DK 2379717 T3 DK2379717 T3 DK 2379717T3
Authority
DK
Denmark
Prior art keywords
enzyme
activity
fermentation
enzyme product
assay
Prior art date
Application number
DK09797043.8T
Other languages
English (en)
Inventor
Mette Zacho Grønfeldt
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Application granted granted Critical
Publication of DK2379717T3 publication Critical patent/DK2379717T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G1/00—Preparation of wine or sparkling wine
    • C12G1/02—Preparation of must from grapes; Must treatment and fermentation
    • C12G1/0203—Preparation of must from grapes; Must treatment and fermentation by microbiological or enzymatic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011—Pectinesterase (3.1.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01067—Galacturan 1,4-alpha-galacturonidase (3.2.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/0201—Pectin lyase (4.2.2.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus

Claims (15)

1. Fremgangsmåde til fremstilling af et enzymprodukt, hvilken fremgangsmåde omfatter trinnene submers fermentering af en Aspergillus tubingensis-stamme med deponeringsadgangsnummer CBS123488 i et fermenteringsmedium, hvilket medium omfatter vegetabilsk materiale omfattende cerealieklid og sukkerroepulp, til opnåelse af en fermenteringssuppe omfattende enzymproduktet.
2. Fremgangsmåde ifølge krav 1, hvori enzymproduktet omfatter i det mindste følgende enzymaktiviteter: i. endo-polygalacturonase-aktivitet, ii. exo-polygalacturonase-aktivitet, iii. pectinesterase-aktivitet, iv. pectin-lyase-aktivitet, v. cellulase-aktivitet, vi. xylanase-aktivitet og vii. arabinanase-aktivitet.
3. Fremgangsmåde ifølge ethvert af kravene 1-2, hvori det vegetabilske materiale i fermenteringsmediet er en blanding af cerealieklid og sukkerroepulp i et forhold på omkring 20:80 til 80:20.
4. Fremgangsmåde ifølge ethvert af kravene 1-3, hvori enzymproduktet udvindes i form af en cellefri suppe fra fermenteringssuppen.
5. Fremgangsmåde ifølge ethvert af kravene 1-4, hvori fermenteringsmediet omfatter sukkerroepulp i intervallet 1-30 vægt/vægt%, 2-8 vægt/vægt% eller 3-7 vægt/vægt%.
6. Fremgangsmåde ifølge ethvert af kravene 1-5, hvori sukkerroepulpen har et sukkerindhold på 0,5-20 vægt/vægt% eller 2-8 vægt/vægt%.
7. Fremgangsmåde ifølge ethvert af kravene 1-6, hvori cerealiekliddet er hvedeklid.
8. Fremgangsmåde ifølge ethvert af kravene 1-7, hvori sukkerroepulpen har et arabinanindhold på 5-40 vægt/vægt% og/eller hvori hvedekliddet har et arabinanindhold på 2-20 vægt/vægt%.
9. Fremgangsmåde ifølge ethvert af kravene 1-8, hvori fermenteringsmediet endvidere omfatter ammoniumsulfat og/eller kaliumnitrat.
10. Enzymprodukt omfattende mindst følgende enzymaktiviteter: i. endo-polygalacturonase-aktivitet ii. exo-polygalacturonase-aktivitet iii. pectinesterase-aktivitet iv. pectin-lyase-aktivitet v. cellulase-aktivitet vi. xylanase-aktivitet vii. arabinanase-aktivitet og hvori enzymproduktet er et ekspressionsprodukt opnået ved submers fermentering af en Aspergillus tubingensis-stamme med deponeringsadgangsnummer CBS123488 i et medium, hvori mediet, som anvendes til den submerse fermentering, omfatter cerealieklid og sukkerroepulp.
11. Enzymproduktpræparat omfattende et enzymprodukt ifølge krav 10, en enzymbærer og eventuelt en stabilisator og/eller et konserveringsmiddel.
12. Anvendelse af et enzymprodukt ifølge krav 10 eller et enzymproduktpræparat ifølge krav 11 til nedbrydning af plantemateriale, som eventuelt er genetisk modificeret, såsom hvori plantematerialet er en plantecelle og/eller hvori plantematerialet er en plantecellevæg eller en del deraf.
13. Anvendelse af et enzymprodukt ifølge krav 10 eller et enzymproduktpræparat ifølge krav 11 til frigivelse af en forbindelse, såsom hvori forbindelsen er en biologisk aktiv forbindelse eller et forstadie derfor, eller såsom hvori forbindelsen er en farmaceutisk aktiv forbindelse.
14. Anvendelse af et enzymprodukt ifølge krav 10 eller et enzymproduktpræparat ifølge krav 11 til nedbrydning af affald.
15. Anvendelse af et enzymprodukt ifølge krav 10 eller et enzymproduktpræparat ifølge krav 11 under fremstilling af frugtsaft og/eller vin i trinnene ekstraktion og/eller flydendegørelse af frugt og/eller frugtsaftklaring.
DK09797043.8T 2008-12-19 2009-12-21 Fremgangsmåde til fremstilling af et enzymprodukt DK2379717T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13949308P 2008-12-19 2008-12-19
EP08172396 2008-12-19
PCT/EP2009/067690 WO2010070146A1 (en) 2008-12-19 2009-12-21 Process for production of an enzyme product

Publications (1)

Publication Number Publication Date
DK2379717T3 true DK2379717T3 (da) 2015-12-14

Family

ID=40512528

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09797043.8T DK2379717T3 (da) 2008-12-19 2009-12-21 Fremgangsmåde til fremstilling af et enzymprodukt

Country Status (6)

Country Link
US (1) US8765442B2 (da)
EP (1) EP2379717B1 (da)
CN (2) CN102292437B (da)
AR (1) AR074835A1 (da)
DK (1) DK2379717T3 (da)
WO (1) WO2010070146A1 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0902012A2 (pt) * 2009-01-08 2010-11-16 Inst Alberto Luiz Coimbra aplicação de extratos de cascas de frutas como inibidores de corrosão e processo de obtenção dos mesmos
WO2012049737A1 (ja) * 2010-10-12 2012-04-19 アサヒグループホールディングス株式会社 デンプン分解酵素活性及び食物繊維分解酵素活性が増強された液体麹の製造方法
BR112013018947A2 (pt) * 2011-01-24 2016-10-04 Buckman Labor Inc método para isolar componentes de material lignocelulósico, sistema para isolar componentes de material lignocelulósico, método para produzir produto de lignina purificada através de tratamento enzimático, produto de lignina purificada ou produto de lignina intermediário e método para separar lignina de açúcar num material lignocelulósico
EP2885407B1 (en) * 2012-08-16 2018-06-13 Bangladesh Jute Research Institute Pectin degrading enzymes from macrophomina phaseolina and uses thereof
GB201218103D0 (en) * 2012-10-09 2012-11-21 Lystek Internat Inc Process for removal of chemical contaminants from biological wastes
CN103409388B (zh) * 2013-04-01 2015-07-08 山东隆科特酶制剂有限公司 含果胶酯酶s6pe-pe和多聚半乳糖醛酸酶s6pe-pg的杂合酶s6pe-a及其基因和应用
FR3016768B1 (fr) * 2014-01-27 2016-02-26 J Soufflet Ets Utilisation d'une composition enzymatique dans l'alimentation des ruminants
CN106306911A (zh) * 2015-06-17 2017-01-11 烟台北方安德利果汁股份有限公司 一种提高出汁率且不影响果胶回收的苹果汁的加工工艺
FR3047999A1 (fr) * 2016-02-23 2017-08-25 Etablissements J Soufflet Biocatalyseur pour la production d'ethanol a partir de mais
CN106434600B (zh) * 2016-09-28 2019-08-09 集美大学 一种塔宾曲霉发酵生产果胶酯酶的方法
CN107151631A (zh) * 2017-03-16 2017-09-12 西北农林科技大学 一种高产果胶酶菌株及其应用
CN107673781A (zh) * 2017-08-24 2018-02-09 广西力源宝科技有限公司 自动进料塔式发酵系统及进料方法
CN107440123A (zh) * 2017-09-12 2017-12-08 广西壮族自治区农业科学院农产品加工研究所 一种浆果速溶粉及其制作方法
WO2019060851A1 (en) * 2017-09-24 2019-03-28 Bio-Cat, Inc. MIXTURES OF FUNGAL PROTEASE AND USES THEREOF
CN109303224A (zh) * 2018-09-28 2019-02-05 烟台北方安德利果汁股份有限公司 一种稳定苹果清汁色值并同时提高超滤通量的加工方法
KR102417221B1 (ko) * 2019-12-02 2022-07-06 에이치유원 주식회사 전복을 포함하는 반려동물용 간식 및 그 제조 방법
CN111549020B (zh) * 2020-06-08 2023-03-28 杭州保安康生物技术有限公司 富含酸性果胶酶的复合酶的制备及其菌株和应用
WO2023102065A1 (en) * 2021-11-30 2023-06-08 Wisconsin Alumni Research Foundation Development of food-grade fungal fermentates with antimicrobial activity and the use thereof
CN114891641B (zh) * 2022-04-02 2023-11-03 中山大学 一种产淀粉酶的菌株

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4136201A (en) * 1967-12-06 1979-01-23 Gb Fermentation Industries, Inc. Microbial rennin
US3925160A (en) * 1974-09-26 1975-12-09 Univ Creighton Method of producing an antibiotic
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
ES2063162T5 (es) 1988-07-28 2002-11-16 Novartis Ag Nuevo sistema de expresion.
DE3908813A1 (de) 1989-03-17 1990-09-20 Roehm Gmbh Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus
EP0421919A3 (en) 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
US5550045A (en) 1992-05-15 1996-08-27 Unilever Patent Holdings, B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalcturonase activity
EP0570075A3 (en) 1992-05-15 1995-02-15 Quest Int Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity.
US20030068805A1 (en) * 1995-03-17 2003-04-10 Susan Madrid Endo beta-1,4-glucanase from aspergillus
US6558728B1 (en) * 1996-05-10 2003-05-06 Danisco A/S α-glucuronidases of aspergillus, production thereof and their uses
EP1114165A1 (en) 1998-09-18 2001-07-11 Dsm N.V. Aspergillus tubigensis polygalacturonase
FR2788782B1 (fr) * 1999-01-25 2003-01-31 Gie Agro Ind Produit multienzymatique a activites glucoamylasique, proteolytique et xylanasique et procede pour sa production par fermentation a l'etat solide de son de ble avec aspergillus niger
ES2331467T3 (es) * 2001-10-30 2010-01-05 Societe Des Produits Nestle S.A. Manipulacion del aroma del chocolate.
DE10339010A1 (de) 2003-08-25 2005-03-24 Süd-Chemie AG Enzymatische Behandlung einer Masse aus Oliven oder Olivenbestandteilen
WO2008117301A1 (en) 2007-03-23 2008-10-02 Council Of Scientific & Industrial Research A process for the production of multienzyme system using fermentation
WO2011124538A1 (en) * 2010-04-06 2011-10-13 Universiteit Gent A thermostable sucrose phosphorylase
JP5903492B2 (ja) * 2011-09-16 2016-04-13 クレド バイオメディカル ピーティーイー リミテッド 分子診断アッセイデバイス及び使用方法

Also Published As

Publication number Publication date
CN102292437B (zh) 2015-06-24
AR074835A1 (es) 2011-02-16
US8765442B2 (en) 2014-07-01
US20120202262A9 (en) 2012-08-09
EP2379717B1 (en) 2015-09-02
CN104480093A (zh) 2015-04-01
WO2010070146A1 (en) 2010-06-24
CN102292437A (zh) 2011-12-21
US20120028332A1 (en) 2012-02-02
EP2379717A1 (en) 2011-10-26

Similar Documents

Publication Publication Date Title
DK2379717T3 (da) Fremgangsmåde til fremstilling af et enzymprodukt
EP2421965B1 (en) Carbohydrate degrading polypeptide and uses thereof
CN102971419B (zh) 具有降解碳水化合物材料的活性或帮助降解碳水化合物材料的活性的多肽及其用途
ES2802807T3 (es) Polipéptido degradador de carbohidratos y sus usos
EP3318574B1 (en) Polypeptide having beta-glucosidase activity and uses thereof
US9133448B2 (en) Polypeptide having cellobiohydrolase activity and uses thereof
EP2588492B1 (en) Polypeptide having beta-glucosidase activity and uses thereof
CN102597213A (zh) 篮状菌转化体
MX2014002950A (es) Composiciones que comprenden enzimas con activiad de endo-1,4-beta-xilanasa y enzimas con actividad de endo-1,3(4)-beta glucanasa.
WO2009133036A1 (en) Cellobiohydrolase 1 from penicillium chysogenum and uses thereof
CN103391999A (zh) 具有乙酰基木聚糖酯酶活性的多肽及其用途
CN103119156A (zh) 具有降解碳水化合物活性的多肽及其用途
WO2009133035A1 (en) Cellobiohydrolase 1 from penicillium chysogenum and uses thereof
CN103180337A (zh) 具有膨胀因子活性的多肽及其用途
Rao et al. Fungal enzymes: present scenario and future perspectives
Adeoyo Bioprospecting for amylases, cellulases and xylanases from ericoid associated fungi, their production and characterisation for the bio-economy
AU2014246633A1 (en) Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof