DK2345716T3 - Rekombinante influenzavira til vacciner og genterapi - Google Patents
Rekombinante influenzavira til vacciner og genterapi Download PDFInfo
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- DK2345716T3 DK2345716T3 DK10011896.7T DK10011896T DK2345716T3 DK 2345716 T3 DK2345716 T3 DK 2345716T3 DK 10011896 T DK10011896 T DK 10011896T DK 2345716 T3 DK2345716 T3 DK 2345716T3
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Claims (15)
1. 1. En celle der bringes i kontakt med en flerhed af orthomyxovirusvektorer der omfatter: (i) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus PA cDNA forbundet med en transkriptionstermineringssekvens, (ii) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus PB 1 cDNA forbundet med en transkriptionstermineringssekvens, (iii) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus PB2 cDNA forbundet med en transkriptionstermineringssekvens, (iv) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus HA cDNA forbundet med en transkriptionstermineringssekvens, (v) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus NP cDNA forbundet med en transkriptionstermineringssekvens, (vi) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus NA cDNA forbundet med en transkriptionstermineringssekvens, (vii) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus M cDNA forbundet med en transkriptionstermineringssekvens, (viii) en vektor der omfatter en promotor som er funktionelt forbundet med et influenzavirus NS cDNA forbundet med en transkriptionstermineringssekvens, (ix) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenza-PA-polypeptid og forbundet med en transkriptionstermineringssekvens, (x) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenza-PBl-polypeptid og forbundet med en transkriptionstermineringssekvens, (xi) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenza-PB2-polypeptid og forbundet med en transkriptionstermineringssekvens, og (xii) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenza-NP-polypeptid og forbundet med en transkriptionstermineringssekvens hvor cellen udtrykker orthomyxovirus.
2. Cellen ifølge krav 1, hvor cellen yderligere bringes i kontakt med en flerhed af orthomyxovirus-vektorer der omfatter: (xiii) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenzavirus HA-polypeptid og forbundet med en transkriptionstermineringssekvens, (xiv) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenzavirus NA-polypeptid og forbundet med en transkriptionstermineringssekvens, (xv) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenzavirus Ml-polypeptid og forbundet med en transkriptionstermineringssekvens, (xvi) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenzavirus M2-polypeptid og forbundet med en transkriptionstermineringssekvens, og (xvii) en vektor der omfatter en promotor som er funktionelt forbundet med et DNA-segment kodende for et influenzavirus NS2-polypeptid og forbundet med en transkriptionstermineringssekvens hvor cellen udtrykker orthomyxovirus.
3. Cellen ifølge et hvilket som helst af kravene 1 til 2, hvor vektorpromotoren er en promotor valgt fra gruppen bestående af en RNA-polymerase I-promotor, en RNA-polymerase Il-promotor, en RNA-polymerase ΙΙΙ-promotor, en T7-promotor, og en T3-promotor.
4. Cellen ifølge et hvilket som helst af kravene 1 til 3, hvor vektorpromotoren er en RNA-polymerase I-promotor.
5. Cellen ifølge et hvilket som helst af kravene 1 til 4, hvor vektorpromotoren er en human RNA-polymerase I-promotor.
6. Cellen ifølge et hvilket som helst af kravene 1 til 5, hvor vektortranskriptionstermineringssekvensen er valgt fra gruppen bestående af en transkriptionstermineringssekvens valgt fra en RNA-polymerase I transkriptionstermineringssekvens, en RNA-polymerase II transkriptionstermineringssekvens, en RNA-polymerase III transkriptionstermineringssekvens, og et ribozym.
7. Cellen ifølge et hvilket som helst af kravene 1 til 6, hvor to eller flere vektorer er forbundet fysisk.
8. Cellen ifølge et hvilket som helst af kravene 1 til 7, hvor én eller flere vektorer er på separate plasmider.
9. Cellen ifølge et hvilket som helst af kravene 1 til 8, der yderligere omfatter trinnet at indføre mindst én mutation i en eller flere af influenza-PA cDNAet, influenza-PBl cDNAet, influenza-PB2 cDNAet, influenza-HA cDNAet, influenza-NP cDNAet, influenza-NA cDNAet, influenza-M cDNAet, eller influenza-NS cDNAet, før indføring af vektoren i værtscellerne.
10. Cellen ifølge et hvilket som helst af kravene 1 til 9, der yderligere omfatter at indføre mindst én mutation i influenza-HA cDNAet før indføring af vektorerne i værtscellerne.
11. Cellen ifølge et hvilket som helst af kravene 1 til 10, hvor én eller flere vektorer yderligere omfatter 3'- og 5'-ikke-kodende sekvens af et influenzavirus.
12. Cellen ifølge et hvilket som helst af kravene 1 til 11, hvor en vektor koder for et immunogent peptid eller protein der kan anvendes som en vaccine.
13. Cellen ifølge et hvilket som helst af kravene 1 til 12, hvor cellerne er pattedyrceller.
14. Cellen ifølge et hvilket som helst af kravene 1 til 13, hvor cDNA'et er i en sense-orientering.
15. Cellen ifølge et hvilket som helst af kravene 1 til 13, hvor cDNAet er i antisense-orientering.
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| DK00920151T DK1185615T3 (da) | 1999-04-06 | 2000-04-05 | Rekombinante influenzavirus til vacciner og genterapi |
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| DK00920151T DK1185615T3 (da) | 1999-04-06 | 2000-04-05 | Rekombinante influenzavirus til vacciner og genterapi |
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| DE122008000061I1 (de) * | 1999-04-06 | 2009-02-12 | Wisconsin Alumni Res Found | Rekombinante influenzaviren zur vakzinherstellung und gentherapie |
| US8715940B2 (en) | 1999-04-06 | 2014-05-06 | Wisconsin Alumni Research Foundation | Method of making recombinant influenza virus |
| DE60033284T3 (de) * | 1999-07-14 | 2014-10-30 | Icahn School Of Medicine At Mount Sinai | In vitro-rekonstitution von segmentierten, negativstrang-rna-viren |
| GB9916794D0 (en) * | 1999-07-16 | 1999-09-22 | Isis Innovation | In vitro virus reconstitution |
| IL152426A (en) | 2000-04-28 | 2011-07-31 | St Jude Childrens Res Hospital | A DNA transfection system to form an infectious negative-strand RNA virus |
| CA2410297C (en) | 2000-06-23 | 2011-03-15 | American Cyanamid Company | Assembly of wild-type and chimeric influenza virus-like particles (vlps) |
| EP1862550A1 (en) * | 2000-06-23 | 2007-12-05 | Wyeth Holdings Corporation | Assembly of wild-type and chimeric influenza virus-like particles (VLPs) |
| US7211378B2 (en) | 2002-01-31 | 2007-05-01 | Wisconsin Alumni Research Foundation | Filovirus vectors and noninfectious filovirus-based particles |
| BRPI0307679A2 (pt) * | 2002-02-13 | 2016-11-08 | Wisconsin Alumni Res Found | vetor viral de influenza, vírus recombinate de influenza, molécula isolada de ácido nucleico e métodos para expressar um segmento heterólogo de ácido nucleico em uma célula e para preparar vírus semelhante a influenza incompetente na replicação. |
| KR101169468B1 (ko) | 2002-04-26 | 2012-08-01 | 메디뮨 엘엘씨 | 인플루엔자 바이러스의 생산을 위한 다중 플라스미드시스템 |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| WO2004112831A2 (en) * | 2003-05-28 | 2004-12-29 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines and gene therapy |
| WO2005018539A2 (en) | 2003-06-16 | 2005-03-03 | Medimmune Vaccines, Inc. | Influenza hemagglutinin and neuraminidase variants |
| CA2551489C (en) | 2003-12-23 | 2013-09-03 | Gregory Duke | Multi plasmid system for the production of influenza virus |
| FR2866031B1 (fr) * | 2004-02-11 | 2012-10-19 | David Francois Joseph Millet | Chimere de virus pathogene emergent, medicament la contenant et utilisation en therapie antivirale |
| DE202005022108U1 (de) | 2004-03-09 | 2013-11-12 | Novartis Vaccines And Diagnostics, Inc. | Influenza-Virus-Impfstoffe |
| CA2879182C (en) | 2004-05-25 | 2017-02-14 | Medimmune, Inc. | Influenza hemagglutinin and neuraminidase variants |
| KR20070086344A (ko) | 2004-11-19 | 2007-08-27 | 위스콘신 얼럼나이 리서어치 화운데이션 | 탠덤 전사 유닛을 갖는 재조합 인플루엔자 벡터 |
| EP2573186A1 (en) | 2004-12-08 | 2013-03-27 | MedImmune, LLC | Methods of producing influenza vaccine compositions |
| BRPI0518568A2 (pt) | 2004-12-24 | 2008-11-25 | Solvay Pharm Bv | mÉtodo para produzir uma partÍcula do vÍrus da influenza replicativo sem o uso de vÍrus auxiliar, partÍcula de vÍrus da influenza replicativo, cÉlula, composiÇço, uso de uma composiÇço, mÉtodo para gerar proteÇço imunolàgica contra infecÇço de um indivÍduo com um vÍrus da influenza, e, Ácido nuclÉico |
| EP1856271A4 (en) | 2005-03-08 | 2009-11-18 | Medimmune Vaccines Inc | INFLUENZA HEMAGGLUTININE AND NEURAMINIDASE VARIANTS |
| US11707520B2 (en) | 2005-11-03 | 2023-07-25 | Seqirus UK Limited | Adjuvanted vaccines with non-virion antigens prepared from influenza viruses grown in cell culture |
| US8697087B2 (en) | 2005-11-04 | 2014-04-15 | Novartis Ag | Influenza vaccines including combinations of particulate adjuvants and immunopotentiators |
| JP2009514839A (ja) | 2005-11-04 | 2009-04-09 | ノバルティス ヴァクシンズ アンド ダイアグノスティクス エスアールエル | サイトカイン誘導剤を含むアジュバントインフルエンザワクチン |
| ES2420829T3 (es) | 2005-11-04 | 2013-08-27 | Novartis Vaccines And Diagnostics S.R.L. | Vacunas adyuvantadas con antígeno de no virión preparadas a partir de virus de la gripe cultivados en cultivo celular |
| KR20080069232A (ko) | 2005-11-04 | 2008-07-25 | 노바티스 백신즈 앤드 다이아그노스틱스 에스.알.엘. | 스플리트 인플루엔자 백신에 대한 보조제로서 유리 수성상계면활성제를 갖는 에멀젼 |
| PL2251034T3 (pl) | 2005-12-02 | 2018-07-31 | Icahn School Of Medicine At Mount Sinai | Chimeryczne wirusy prezentujące nienatywne białka powierzchniowe i zastosowania tych wirusów |
| EA015271B1 (ru) | 2006-01-27 | 2011-06-30 | Новартис Вэксинс Энд Диагностикс Гмбх & Ко Кг | Противогриппозные вакцины, содержащие гемагглютинин и белки матрикса |
| EP2004226A1 (en) | 2006-03-24 | 2008-12-24 | Novartis Vaccines and Diagnostics GmbH & Co. KG | Storage of influenza vaccines without refrigeration |
| US9254318B2 (en) | 2006-03-31 | 2016-02-09 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses for vaccines |
| US20090017444A1 (en) * | 2006-06-09 | 2009-01-15 | Wisconsin Alumni Research Foundation | Screening method for modulators of viral transcription or replication |
| GB0614460D0 (en) | 2006-07-20 | 2006-08-30 | Novartis Ag | Vaccines |
| EP2049662A4 (en) | 2006-07-21 | 2009-12-30 | Medimmune Llc | METHODS AND COMPOSITIONS FOR INCREASING REPLICATION CAPACITY OF A FLU VIRUS |
| CN101983069B (zh) | 2006-08-09 | 2014-07-16 | 米迪缪尼有限公司 | 流感血凝素和神经氨酸酶变体 |
| KR20090057015A (ko) | 2006-09-11 | 2009-06-03 | 노파르티스 아게 | 난을 사용하지 않는 인플루엔자 바이러스 백신의 제조 |
| US20110045022A1 (en) | 2006-12-06 | 2011-02-24 | Theodore Tsai | Vaccines including antigen from four strains of influenza virus |
| US8597661B2 (en) | 2007-05-04 | 2013-12-03 | Wisconsin Alumni Research Foundation | Neuraminidase-deficient live influenza vaccines |
| US9474798B2 (en) | 2007-06-18 | 2016-10-25 | Wisconsin Alumni Research Foundation | Influenza M2 protein mutant viruses as live influenza attenuated vaccines |
| CA2690196A1 (en) | 2007-06-18 | 2008-12-24 | Medimmune, Llc | Influenza b viruses having alterations in the hemaglutinin polypeptide |
| PL2185191T3 (pl) | 2007-06-27 | 2013-02-28 | Novartis Ag | Szczepionki przeciwko grypie o małej zawartości dodatków |
| EP2045323A1 (en) * | 2007-10-05 | 2009-04-08 | Avir Green Hills Biotechnology Research Development Trade Ag | Linear expression constructs for production of influenza virus particles |
| CN101497877B (zh) * | 2008-01-28 | 2012-09-05 | 河北摩百生物科技有限公司 | 流感全病毒减活疫苗重组体、其构建方法及应用 |
| EA201071086A1 (ru) | 2008-03-18 | 2011-04-29 | Новартис Аг | Усовершенствованный способ получения вакцинных антигенов вируса гриппа |
| RU2523587C2 (ru) | 2008-07-11 | 2014-07-20 | МЕДИММЬЮН, ЭлЭлСи | Варианты гемагглютинина и нейрамидазы вируса гриппа |
| AU2009283557B2 (en) | 2008-08-18 | 2013-01-17 | Kitasato Daiichi Sankyo Vaccine Co., Ltd. | Avian influenza virus antigen, and booster immunization method for avian influenza vaccine in combination with mucosal adjuvant which is effective through oral administration |
| EP2233568A1 (en) | 2009-03-19 | 2010-09-29 | Avir Green Hills Biotechnology Research Development Trade AG | Novel method for generation of RNA virus |
| CA2752041A1 (en) | 2009-02-10 | 2010-08-19 | Novartis Ag | Influenza vaccines with increased amounts of h3 antigen |
| CN102307590A (zh) | 2009-02-10 | 2012-01-04 | 诺华有限公司 | 具有减少量的角鲨烯的流感疫苗 |
| EA201171032A1 (ru) | 2009-02-10 | 2012-02-28 | Новартис Аг | Схемы лечения с помощью вакцины против гриппа, связанного с пандемическими штаммами |
| SG2014010185A (en) | 2009-02-12 | 2014-06-27 | Medimmune Llc | Influenza hemagglutinin and neuraminidase variants |
| USH2283H1 (en) | 2009-04-27 | 2013-09-03 | Novartis Ag | Vaccines for protecting against influenza |
| BR112012008338A2 (pt) | 2009-09-10 | 2019-09-24 | Novartis Ag | combinação de vacinas contra doenças do trato respiratório. |
| US9109013B2 (en) | 2009-10-26 | 2015-08-18 | Wisconsin Alumni Research Foundation | High titer recombinant influenza viruses with enhanced replication in vero cells |
| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| EP2576773A2 (en) | 2010-06-02 | 2013-04-10 | Avir Green Hills Biotechnology Research Development Trade AG | Methods and helper viruses for the generation of rna virus |
| CN102220293B (zh) * | 2011-05-26 | 2012-12-19 | 中国农业科学院上海兽医研究所 | 犬流感重组病毒及其制备方法和应用 |
| EP2747778B1 (en) | 2011-08-26 | 2017-12-06 | Wisconsin Alumni Research Foundation | Influenza viruses with mutant pb2 gene segment as live attenuated vaccines |
| EP2751260A2 (en) | 2011-09-02 | 2014-07-09 | Westfälische Wilhelms-Universität Münster | Live attenuated influenza virus |
| AU2012324398A1 (en) | 2011-10-20 | 2014-05-01 | Seqirus UK Limited | Adjuvanted influenza B virus vaccines for pediatric priming |
| GB201218195D0 (en) | 2012-10-10 | 2012-11-21 | Istituto Zooprofilattico Sperimentale Delle Venezie | Composition |
| JP2016524915A (ja) | 2013-07-15 | 2016-08-22 | ウィスコンシン アルムニ リサーチ ファンデイション | Ｍｄｃｋ、ベロ細胞又は卵内で増強された複製を有する高力価の組換えインフルエンザウイルス |
| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| CN104450695B (zh) * | 2014-11-26 | 2017-08-25 | 扬州大学 | A型流感病毒基因pcr扩增引物及其快速克隆方法 |
| US10633422B2 (en) | 2015-06-01 | 2020-04-28 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication by inhibiting microRNA lec7C binding to influenza viral cRNA and mRNA |
| JP7098330B2 (ja) * | 2015-06-10 | 2022-07-11 | ホオキパ バイオテック ジーエムビーエイチ | Ｈｐｖワクチン |
| WO2016207853A2 (en) | 2015-06-26 | 2016-12-29 | Seqirus UK Limited | Antigenically matched influenza vaccines |
| US9890363B2 (en) | 2015-07-06 | 2018-02-13 | Wisconsin Alumni Research Foundation (Warf) | Influenza virus replication for vaccine development |
| CN108027371B (zh) | 2015-07-07 | 2020-08-18 | 思齐乐 | 流感效力试验 |
| EP3341018A1 (en) | 2015-08-28 | 2018-07-04 | Wisconsin Alumni Research Foundation | Generation of infectious influenza viruses from virus-like particles |
| US11197925B2 (en) | 2016-02-19 | 2021-12-14 | Wisconsin Alumni Research Foundation (Warf) | Influenza B virus replication for vaccine development |
| US11013695B2 (en) * | 2017-08-28 | 2021-05-25 | Wisconsin Alumni Research Foundation | Nanocapsule delivery system for ribonucleoproteins |
| CN107964035B (zh) * | 2017-12-06 | 2020-07-24 | 中国科学院微生物研究所 | 一种用于流感病毒的有限复制型黏膜免疫疫苗 |
| CN109097341B (zh) * | 2018-08-28 | 2021-09-24 | 青岛农业大学 | 一种同时表达ha和hef的复制缺陷型重组流感病毒 |
| JP2022527235A (ja) | 2019-01-23 | 2022-06-01 | 義裕 河岡 | インフルエンザウイルスにおける付加的遺伝子に遺伝的安定性を付与する突然変異 |
| CN113874496A (zh) | 2019-02-08 | 2021-12-31 | 威斯康星校友研究基金会(Warf) | 人源化细胞系 |
| CN114929269A (zh) | 2019-05-01 | 2022-08-19 | 威斯康星校友研究基金会(Warf) | 用于疫苗开发的改进的流感病毒复制 |
| US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840520A (en) * | 1989-08-28 | 1998-11-24 | Aviron | Recombinant negative strand RNA virus expression systems |
| US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
| US5919676A (en) * | 1993-06-24 | 1999-07-06 | Advec, Inc. | Adenoviral vector system comprising Cre-loxP recombination |
| EP0704533A1 (en) * | 1994-09-30 | 1996-04-03 | Bayer Ag | An attenuated vaccination virus, a method to make the virus and a pharmaceutical compositions comprising the virus |
| WO1996010633A1 (en) * | 1994-09-30 | 1996-04-11 | Aviron | Chimeric influenza virus and electroporation method |
| CN1200675A (zh) * | 1995-10-20 | 1998-12-02 | 耶路撒冷希伯来语大学依苏姆研究开发公司 | 流感疫苗 |
| US5994526A (en) * | 1996-06-21 | 1999-11-30 | Plant Genetic Systems | Gene expression in plants |
| EP1035209A1 (en) * | 1999-03-06 | 2000-09-13 | ARTEMIS Pharmaceuticals GmbH | Stable recombinant influenza viruses free of helper viruses |
| DE122008000061I1 (de) * | 1999-04-06 | 2009-02-12 | Wisconsin Alumni Res Found | Rekombinante influenzaviren zur vakzinherstellung und gentherapie |
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