DK2305826T3 - Materials and methods for the efficient production of lactic acid - Google Patents
Materials and methods for the efficient production of lactic acid Download PDFInfo
- Publication number
- DK2305826T3 DK2305826T3 DK10186896.6T DK10186896T DK2305826T3 DK 2305826 T3 DK2305826 T3 DK 2305826T3 DK 10186896 T DK10186896 T DK 10186896T DK 2305826 T3 DK2305826 T3 DK 2305826T3
- Authority
- DK
- Denmark
- Prior art keywords
- lactate
- lactic acid
- strain
- coli
- dna
- Prior art date
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims description 137
- 238000004519 manufacturing process Methods 0.000 title claims description 78
- 239000004310 lactic acid Substances 0.000 title claims description 65
- 235000014655 lactic acid Nutrition 0.000 title claims description 65
- 238000000034 method Methods 0.000 title claims description 52
- 239000000463 material Substances 0.000 title description 4
- 241000588724 Escherichia coli Species 0.000 claims description 81
- 238000012217 deletion Methods 0.000 claims description 46
- 230000037430 deletion Effects 0.000 claims description 46
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 claims description 40
- 101150083023 mgsA gene Proteins 0.000 claims description 18
- 101100139916 Escherichia coli (strain K12) rarA gene Proteins 0.000 claims description 13
- 101100433987 Latilactobacillus sakei subsp. sakei (strain 23K) ackA1 gene Proteins 0.000 claims description 11
- 101150006213 ackA gene Proteins 0.000 claims description 11
- 230000002779 inactivation Effects 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 108010092060 Acetate kinase Proteins 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 108010012901 Succinate Dehydrogenase Proteins 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 108010083856 methylglyoxal synthase Proteins 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 description 81
- 230000004151 fermentation Effects 0.000 description 81
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 76
- 108090000623 proteins and genes Proteins 0.000 description 72
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 64
- 239000008103 glucose Substances 0.000 description 64
- 239000002609 medium Substances 0.000 description 60
- 108020004414 DNA Proteins 0.000 description 57
- 229910052500 inorganic mineral Inorganic materials 0.000 description 46
- 239000011707 mineral Substances 0.000 description 46
- 235000010755 mineral Nutrition 0.000 description 46
- 150000003839 salts Chemical class 0.000 description 46
- 239000002773 nucleotide Substances 0.000 description 45
- 125000003729 nucleotide group Chemical group 0.000 description 45
- 229930006000 Sucrose Natural products 0.000 description 42
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 42
- 210000004027 cell Anatomy 0.000 description 42
- 239000005720 sucrose Substances 0.000 description 42
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 40
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 33
- 244000005700 microbiome Species 0.000 description 33
- 239000013612 plasmid Substances 0.000 description 32
- 235000000346 sugar Nutrition 0.000 description 31
- 230000000692 anti-sense effect Effects 0.000 description 29
- 230000012010 growth Effects 0.000 description 28
- 239000000047 product Substances 0.000 description 26
- JVTAAEKCZFNVCJ-REOHCLBHSA-M (S)-lactate Chemical compound C[C@H](O)C([O-])=O JVTAAEKCZFNVCJ-REOHCLBHSA-M 0.000 description 24
- 231100000241 scar Toxicity 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 21
- 229960003237 betaine Drugs 0.000 description 20
- 238000010276 construction Methods 0.000 description 20
- 238000010367 cloning Methods 0.000 description 19
- 239000013598 vector Substances 0.000 description 18
- 230000003115 biocidal effect Effects 0.000 description 17
- 230000010354 integration Effects 0.000 description 17
- 241000588749 Klebsiella oxytoca Species 0.000 description 14
- 101150014383 adhE gene Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 13
- 235000010633 broth Nutrition 0.000 description 13
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 12
- 230000002503 metabolic effect Effects 0.000 description 12
- 230000036961 partial effect Effects 0.000 description 12
- 238000012546 transfer Methods 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 241000588700 Dickeya chrysanthemi Species 0.000 description 11
- 239000011942 biocatalyst Substances 0.000 description 11
- 101150070629 celY gene Proteins 0.000 description 11
- 239000007320 rich medium Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 238000003780 insertion Methods 0.000 description 10
- 230000037431 insertion Effects 0.000 description 10
- 230000035772 mutation Effects 0.000 description 10
- 239000006137 Luria-Bertani broth Substances 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 150000007524 organic acids Chemical class 0.000 description 9
- 108010046276 FLP recombinase Proteins 0.000 description 8
- AIJULSRZWUXGPQ-UHFFFAOYSA-N Methylglyoxal Chemical compound CC(=O)C=O AIJULSRZWUXGPQ-UHFFFAOYSA-N 0.000 description 8
- 230000002759 chromosomal effect Effects 0.000 description 8
- 239000002054 inoculum Substances 0.000 description 8
- 230000037361 pathway Effects 0.000 description 8
- 101150025220 sacB gene Proteins 0.000 description 8
- 241000588722 Escherichia Species 0.000 description 7
- 101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 description 7
- 241000588902 Zymomonas mobilis Species 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 101150075213 frdA gene Proteins 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 101150066555 lacZ gene Proteins 0.000 description 7
- 230000004060 metabolic process Effects 0.000 description 7
- 108091008146 restriction endonucleases Proteins 0.000 description 7
- 101100325906 Bacillus subtilis (strain 168) ganA gene Proteins 0.000 description 6
- 208000032544 Cicatrix Diseases 0.000 description 6
- 241001522878 Escherichia coli B Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 101100111413 Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) lacZ gene Proteins 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 101150086432 lacA gene Proteins 0.000 description 6
- 229920000747 poly(lactic acid) Polymers 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 230000037387 scars Effects 0.000 description 6
- 238000010361 transduction Methods 0.000 description 6
- 230000026683 transduction Effects 0.000 description 6
- 239000012137 tryptone Substances 0.000 description 6
- 239000012138 yeast extract Substances 0.000 description 6
- 241000186146 Brevibacterium Species 0.000 description 5
- 241000589565 Flavobacterium Species 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 230000002378 acidificating effect Effects 0.000 description 5
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000001419 dependent effect Effects 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- 229960000318 kanamycin Drugs 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 229930182823 kanamycin A Natural products 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- -1 pentose sugars Chemical class 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 4
- 206010061764 Chromosomal deletion Diseases 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 101710088194 Dehydrogenase Proteins 0.000 description 4
- 241001646716 Escherichia coli K-12 Species 0.000 description 4
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 4
- 239000007993 MOPS buffer Substances 0.000 description 4
- 241000187561 Rhodococcus erythropolis Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 4
- 238000012224 gene deletion Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 230000003472 neutralizing effect Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 239000004626 polylactic acid Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UWTATZPHSA-M (R)-lactate Chemical compound C[C@@H](O)C([O-])=O JVTAAEKCZFNVCJ-UWTATZPHSA-M 0.000 description 3
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 241000590020 Achromobacter Species 0.000 description 3
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 3
- 108010078791 Carrier Proteins Proteins 0.000 description 3
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 3
- 229930182843 D-Lactic acid Natural products 0.000 description 3
- 230000004544 DNA amplification Effects 0.000 description 3
- 101100169312 Escherichia coli (strain K12) cynX gene Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 3
- 108010011939 Pyruvate Decarboxylase Proteins 0.000 description 3
- 240000005384 Rhizopus oryzae Species 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229960005091 chloramphenicol Drugs 0.000 description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
- 229940022769 d- lactic acid Drugs 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 230000006692 glycolytic flux Effects 0.000 description 3
- 238000009776 industrial production Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000037353 metabolic pathway Effects 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000000638 solvent extraction Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- ALRHLSYJTWAHJZ-UHFFFAOYSA-N 3-hydroxypropionic acid Chemical compound OCCC(O)=O ALRHLSYJTWAHJZ-UHFFFAOYSA-N 0.000 description 2
- 230000002407 ATP formation Effects 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 241000186216 Corynebacterium Species 0.000 description 2
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 2
- RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 2
- 240000002605 Lactobacillus helveticus Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 241000191998 Pediococcus acidilactici Species 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- 241000187562 Rhodococcus sp. Species 0.000 description 2
- 101001010097 Shigella phage SfV Bactoprenol-linked glucose translocase Proteins 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- LZCLXQDLBQLTDK-UHFFFAOYSA-N ethyl 2-hydroxypropanoate Chemical compound CCOC(=O)C(C)O LZCLXQDLBQLTDK-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 101150018523 frdB gene Proteins 0.000 description 2
- 101150087653 frdC gene Proteins 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000003209 gene knockout Methods 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 230000005017 genetic modification Effects 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 150000002402 hexoses Chemical class 0.000 description 2
- BSABBBMNWQWLLU-UHFFFAOYSA-N lactaldehyde Chemical compound CC(O)C=O BSABBBMNWQWLLU-UHFFFAOYSA-N 0.000 description 2
- 150000003903 lactic acid esters Chemical class 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 230000000065 osmolyte Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 230000027086 plasmid maintenance Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 229910021654 trace metal Inorganic materials 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- 235000005074 zinc chloride Nutrition 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- KVZLHPXEUGJPAH-HSHFZTNMSA-N 2-hydroxypropanoic acid;(2r)-2-hydroxypropanoic acid Chemical compound CC(O)C(O)=O.C[C@@H](O)C(O)=O KVZLHPXEUGJPAH-HSHFZTNMSA-N 0.000 description 1
- GMVPRGQOIOIIMI-DODZYUBVSA-N 7-[(1R,2R,3R)-3-hydroxy-2-[(3S)-3-hydroxyoct-1-enyl]-5-oxocyclopentyl]heptanoic acid Chemical compound CCCCC[C@H](O)C=C[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DODZYUBVSA-N 0.000 description 1
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000187254 Actinomadura madurae Species 0.000 description 1
- 241000607525 Aeromonas salmonicida Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000588813 Alcaligenes faecalis Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000185996 Arthrobacter citreus Species 0.000 description 1
- 241000193752 Bacillus circulans Species 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 244000177578 Bacterium linens Species 0.000 description 1
- 235000012539 Bacterium linens Nutrition 0.000 description 1
- 241001430265 Beijerinckia indica subsp. indica Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000218649 Brevibacterium fuscum Species 0.000 description 1
- 241001236093 Bulbophyllum maximum Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000588919 Citrobacter freundii Species 0.000 description 1
- 229910021592 Copper(II) chloride Inorganic materials 0.000 description 1
- 241001517047 Corynebacterium acetoacidophilum Species 0.000 description 1
- 241000186145 Corynebacterium ammoniagenes Species 0.000 description 1
- 241000186248 Corynebacterium callunae Species 0.000 description 1
- 241000983382 Curtobacterium pusillum Species 0.000 description 1
- 108010001539 D-lactate dehydrogenase Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102100023319 Dihydrolipoyl dehydrogenase, mitochondrial Human genes 0.000 description 1
- 241000589566 Elizabethkingia meningoseptica Species 0.000 description 1
- 241000589586 Empedobacter brevis Species 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 241000588694 Erwinia amylovora Species 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 108091092584 GDNA Proteins 0.000 description 1
- 241001518248 Gluconobacter cerinus Species 0.000 description 1
- 241000589232 Gluconobacter oxydans Species 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000204057 Kitasatospora Species 0.000 description 1
- 241000588915 Klebsiella aerogenes Species 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 241000144155 Microbacterium ammoniaphilum Species 0.000 description 1
- 241000983412 Microbacterium saperdae Species 0.000 description 1
- 241000203815 Microbacterium testaceum Species 0.000 description 1
- 241000191936 Micrococcus sp. Species 0.000 description 1
- 108010041817 Monocarboxylic Acid Transporters Proteins 0.000 description 1
- 241000588772 Morganella morganii Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000227676 Paenibacillus thiaminolyticus Species 0.000 description 1
- 241000588912 Pantoea agglomerans Species 0.000 description 1
- 241000588701 Pectobacterium carotovorum Species 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 241000364057 Peoria Species 0.000 description 1
- 241000193804 Planococcus <bacterium> Species 0.000 description 1
- 241000157304 Prauserella rugosa Species 0.000 description 1
- 241000186334 Propionibacterium freudenreichii subsp. shermanii Species 0.000 description 1
- 241000588777 Providencia rettgeri Species 0.000 description 1
- 241000185994 Pseudarthrobacter oxydans Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000218935 Pseudomonas azotoformans Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000204709 Pseudomonas mucidolens Species 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 241000589614 Pseudomonas stutzeri Species 0.000 description 1
- 241000218902 Pseudomonas synxantha Species 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 241001524101 Rhodococcus opacus Species 0.000 description 1
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 241000186652 Sporosarcina ureae Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000186988 Streptomyces antibioticus Species 0.000 description 1
- 241000186990 Streptomyces cacaoi Species 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 241000187435 Streptomyces griseolus Species 0.000 description 1
- 241000187389 Streptomyces lavendulae Species 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- 241000218589 Streptomyces olivaceus Species 0.000 description 1
- 241000946755 Streptomyces tanashiensis Species 0.000 description 1
- 241000946734 Streptomyces violaceochromogenes Species 0.000 description 1
- 241000187122 Streptomyces virginiae Species 0.000 description 1
- 241000187191 Streptomyces viridochromogenes Species 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 102000003673 Symporters Human genes 0.000 description 1
- 108090000088 Symporters Proteins 0.000 description 1
- 241000866060 Terrabacter tumescens Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 241001135144 Vibrio metschnikovii Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 241000319304 [Brevibacterium] flavum Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229940005347 alcaligenes faecalis Drugs 0.000 description 1
- PYMYPHUHKUWMLA-YUPRTTJUSA-N aldehydo-L-lyxose Chemical compound OC[C@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-YUPRTTJUSA-N 0.000 description 1
- PNNNRSAQSRJVSB-BXKVDMCESA-N aldehydo-L-rhamnose Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O PNNNRSAQSRJVSB-BXKVDMCESA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011116 calcium hydroxide Nutrition 0.000 description 1
- 230000025938 carbohydrate utilization Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 101150055766 cat gene Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 description 1
- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 1
- 230000005574 cross-species transmission Effects 0.000 description 1
- 101150018392 cscA gene Proteins 0.000 description 1
- 101150091121 cscR gene Proteins 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940092559 enterobacter aerogenes Drugs 0.000 description 1
- 230000007515 enzymatic degradation Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 229940116333 ethyl lactate Drugs 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 101150077334 focA gene Proteins 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 101150023570 frdD gene Proteins 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 235000003869 genetically modified organism Nutrition 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 150000004679 hydroxides Chemical class 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 229940116871 l-lactate Drugs 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940054346 lactobacillus helveticus Drugs 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 239000006151 minimal media Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229940076266 morganella morganii Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 239000013520 petroleum-based product Substances 0.000 description 1
- 101150070013 pfl gene Proteins 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 101150004519 pilC gene Proteins 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000013376 serial cultivation Methods 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000000707 stereoselective effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70688705P | 2005-08-10 | 2005-08-10 | |
| US76157606P | 2006-01-24 | 2006-01-24 | |
| US79961906P | 2006-05-11 | 2006-05-11 | |
| EP06118331A EP1760156B1 (en) | 2005-08-10 | 2006-08-02 | Materials and methods for efficient lactic acid production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2305826T3 true DK2305826T3 (en) | 2016-06-27 |
Family
ID=37488414
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10186896.6T DK2305826T3 (en) | 2005-08-10 | 2006-08-02 | Materials and methods for the efficient production of lactic acid |
| DK06118331.5T DK1760156T3 (da) | 2005-08-10 | 2006-08-02 | Materialer og fremgangsmåder til effektiv mælkesyrefremstilling |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK06118331.5T DK1760156T3 (da) | 2005-08-10 | 2006-08-02 | Materialer og fremgangsmåder til effektiv mælkesyrefremstilling |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US7629162B2 (enExample) |
| EP (2) | EP2305826B1 (enExample) |
| JP (2) | JP4991205B2 (enExample) |
| CN (2) | CN1912107B (enExample) |
| BR (1) | BRPI0603072A (enExample) |
| DK (2) | DK2305826T3 (enExample) |
| ES (2) | ES2573980T3 (enExample) |
| PL (2) | PL1760156T3 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011123154A2 (en) | 2009-11-18 | 2011-10-06 | Myriant Technologies Llc | Metabolic evolution of escherchia coli strains that produce organic acids |
| AU2003287625A1 (en) | 2002-11-06 | 2004-06-03 | University Of Florida | Materials and methods for the efficient production of acetate and other products |
| WO2005033324A1 (ja) * | 2003-09-30 | 2005-04-14 | Mitsui Chemicals, Inc. | Ｄ−乳酸菌生産用生体触媒 |
| DK2305826T3 (en) * | 2005-08-10 | 2016-06-27 | Univ Florida | Materials and methods for the efficient production of lactic acid |
| AU2008228948B2 (en) * | 2007-03-20 | 2014-04-24 | University Of Florida Research Foundation, Inc. | Materials and methods for efficient succinate and malate production |
| US20090082600A1 (en) * | 2007-06-06 | 2009-03-26 | Shengde Zhou | Native homoethanol Pathway for ethanol production in E. coli |
| US8129154B2 (en) | 2008-06-17 | 2012-03-06 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate |
| EP2336295B1 (en) | 2008-09-16 | 2018-01-24 | Mitsui Chemicals, Inc. | Method for producing lactic acid from plant-derived raw material, and lactic-acid-producing bacterium |
| WO2010059616A2 (en) * | 2008-11-18 | 2010-05-27 | University Of Florida Research Foundation, Inc. | Biocatalysts and methods for conversion of hemicellulose hydrolsates to biobased products |
| US8715972B2 (en) * | 2009-02-18 | 2014-05-06 | Bio-Energy Corporation | Homolactic fermentation from pentose |
| KR20120025450A (ko) | 2009-04-02 | 2012-03-15 | 유니버시티 오브 플로리다 리서치 파운데이션, 인크. | 석시네이트 생성을 위한 경로의 엔지니어링 |
| WO2011005554A2 (en) * | 2009-06-23 | 2011-01-13 | Verenium Corporation | Recombinant ethanologenic bacteria |
| BR112012002153A2 (pt) | 2009-07-30 | 2015-09-15 | Metabolic Explorer Sa | metilglioxal sintase mutante (mgs) para a produção de material bioquímico por fermentação |
| US8778656B2 (en) | 2009-11-18 | 2014-07-15 | Myriant Corporation | Organic acid production in microorganisms by combined reductive and oxidative tricaboxylic acid cylce pathways |
| US10017793B2 (en) | 2009-11-18 | 2018-07-10 | Myriant Corporation | Metabolic evolution of Escherichia coli strains that produce organic acids |
| KR20160114184A (ko) | 2009-11-18 | 2016-10-04 | 미리안트 코포레이션 | 화합물들의 효과적인 생산을 위한 미생물 엔지니어링 |
| EP3287519B1 (en) | 2010-11-22 | 2020-03-18 | Cargill, Incorporated | Compositions and methods for 3-hydroxypropionic acid production |
| US9347077B2 (en) | 2011-07-08 | 2016-05-24 | University Of Florida Research Foundation, Incorporated | Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance |
| BR112014001371B1 (pt) | 2011-07-22 | 2021-06-01 | Myriant Corporation | Bactéria escherichia coli geneticamente modificada e método para produção de ácido succínico |
| WO2014017469A1 (ja) * | 2012-07-23 | 2014-01-30 | 三井化学株式会社 | Ｄ－乳酸の生産方法、ポリマーの生産方法およびポリマー |
| AU2013352418B2 (en) | 2012-11-30 | 2016-12-08 | Novozymes, Inc. | 3-Hydroxypropionic Acid production by recombinant yeasts |
| KR20140075044A (ko) | 2012-12-07 | 2014-06-19 | 삼성전자주식회사 | 불포화 카르복실산의 제조방법 |
| CN104278003B (zh) * | 2013-07-12 | 2018-12-18 | 中国科学院天津工业生物技术研究所 | 生产d-乳酸的重组大肠杆菌及其应用 |
| DK3027733T3 (en) | 2013-07-31 | 2018-12-10 | Novozymes As | Preparation of 3-Hydroxypropionic Acid in Recombinant Yeast Expressing an Insect Aspartate-1 Decarboxylase |
| EP2977471A1 (en) * | 2014-07-23 | 2016-01-27 | PURAC Biochem BV | Genetic modification of (S)-lactic acid producing thermophilic bacteria |
| AU2015294136C9 (en) * | 2014-07-23 | 2019-05-02 | Purac Biochem Bv | Genetically modified (R)-lactic acid producing thermophilic bacteria |
| KR102581464B1 (ko) | 2015-05-28 | 2023-09-21 | 삼성전자주식회사 | 락테이트 데히드로게나제 변이체를 스크리닝하는 방법, 락테이트 데히드로게나제 변이체, 상기 변이체를 포함하는 폴리뉴클레오티드, 벡터, 미생물, 및 상기 미생물을 이용하여 락테이트를 생산하는 방법 |
| JP2017121184A (ja) * | 2016-01-04 | 2017-07-13 | 花王株式会社 | 変異リゾプス属菌 |
| WO2019159011A2 (en) | 2018-02-16 | 2019-08-22 | Ptt Global Chemical Public Company Limited | Microorganisms and processes for lactic acid production |
| CN109280652A (zh) * | 2018-09-13 | 2019-01-29 | 四川自豪时代药业有限公司 | 一种外源高表达乳酸脱氢酶突变体的高密度发酵方法 |
| CN109321590B (zh) * | 2018-10-19 | 2022-06-21 | 北京化工大学 | 利用乙酸生产l-乳酸的基因工程菌及其构建方法和应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821093A (en) * | 1988-08-31 | 1998-10-13 | University Of Florida Research Foundation, Inc. | Recombinant cells that highly express chromosomally-integrated heterologous genes |
| US4963486A (en) | 1989-04-21 | 1990-10-16 | Cornell Research Foundation, Inc. | Direct fermentation of corn to L(+)-lactic acid by Rhizopus oryzae |
| CN1156180A (zh) * | 1995-09-05 | 1997-08-06 | 底古萨股份公司 | 用大肠杆菌生产色氨酸 |
| IT1294728B1 (it) | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| US6849444B2 (en) | 2001-04-26 | 2005-02-01 | Archer Daniels Midland Company | Strains of Rhizopus oryzae and uses thereof |
| JP2005528106A (ja) | 2002-05-30 | 2005-09-22 | カーギル ダウ エルエルシー | 酵母における乳酸の生産方法及びその材料 |
| US7300787B2 (en) | 2002-07-05 | 2007-11-27 | Archer-Daniels-Midland Company | Lactobacillus strains and use thereof in fermentation for L-lactic acid production |
| AU2003287625A1 (en) * | 2002-11-06 | 2004-06-03 | University Of Florida | Materials and methods for the efficient production of acetate and other products |
| US20050112737A1 (en) | 2003-11-20 | 2005-05-26 | A. E. Staley Manufacturing Co. | Lactic acid producing yeast |
| DK2305826T3 (en) * | 2005-08-10 | 2016-06-27 | Univ Florida | Materials and methods for the efficient production of lactic acid |
-
2006
- 2006-08-02 DK DK10186896.6T patent/DK2305826T3/en active
- 2006-08-02 ES ES10186896.6T patent/ES2573980T3/es active Active
- 2006-08-02 EP EP10186896.6A patent/EP2305826B1/en not_active Not-in-force
- 2006-08-02 PL PL06118331T patent/PL1760156T3/pl unknown
- 2006-08-02 DK DK06118331.5T patent/DK1760156T3/da active
- 2006-08-02 PL PL10186896.6T patent/PL2305826T3/pl unknown
- 2006-08-02 ES ES06118331T patent/ES2401339T3/es active Active
- 2006-08-02 EP EP06118331A patent/EP1760156B1/en not_active Not-in-force
- 2006-08-08 US US11/501,137 patent/US7629162B2/en not_active Expired - Fee Related
- 2006-08-09 JP JP2006217580A patent/JP4991205B2/ja not_active Expired - Fee Related
- 2006-08-09 BR BRPI0603072-6A patent/BRPI0603072A/pt not_active Application Discontinuation
- 2006-08-10 CN CN2006101093329A patent/CN1912107B/zh not_active Expired - Fee Related
- 2006-08-10 CN CN201310012065.3A patent/CN103396974B/zh not_active Expired - Fee Related
-
2009
- 2009-12-07 US US12/632,041 patent/US8426191B2/en not_active Expired - Fee Related
-
2011
- 2011-10-20 JP JP2011230342A patent/JP5798441B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1912107B (zh) | 2013-03-13 |
| CN103396974A (zh) | 2013-11-20 |
| JP4991205B2 (ja) | 2012-08-01 |
| ES2573980T3 (es) | 2016-06-13 |
| EP2305826A1 (en) | 2011-04-06 |
| DK1760156T3 (da) | 2013-03-18 |
| EP1760156A9 (en) | 2007-04-25 |
| EP1760156B1 (en) | 2012-12-19 |
| CN103396974B (zh) | 2016-12-07 |
| PL2305826T3 (pl) | 2016-10-31 |
| EP2305826B1 (en) | 2016-03-30 |
| EP1760156A2 (en) | 2007-03-07 |
| US20070037265A1 (en) | 2007-02-15 |
| EP1760156A3 (en) | 2007-04-04 |
| US8426191B2 (en) | 2013-04-23 |
| PL1760156T3 (pl) | 2013-05-31 |
| JP5798441B2 (ja) | 2015-10-21 |
| ES2401339T3 (es) | 2013-04-18 |
| JP2012010715A (ja) | 2012-01-19 |
| US7629162B2 (en) | 2009-12-08 |
| CN1912107A (zh) | 2007-02-14 |
| BRPI0603072A (pt) | 2007-03-27 |
| JP2007049993A (ja) | 2007-03-01 |
| US20100203602A1 (en) | 2010-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2305826T3 (en) | Materials and methods for the efficient production of lactic acid | |
| US8691539B2 (en) | Materials and methods for efficient succinate and malate production | |
| Jantama et al. | Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate | |
| Zhang et al. | Production of L-alanine by metabolically engineered Escherichia coli | |
| AU2016201951B2 (en) | Engineering the pathway for succinate production | |
| WO2008119009A2 (en) | Materials and methods for efficient alanine production | |
| US8778656B2 (en) | Organic acid production in microorganisms by combined reductive and oxidative tricaboxylic acid cylce pathways | |
| CN102618478B (zh) | 一株产d-乳酸动态调控重组菌及用其制备d-乳酸的方法 | |
| HK1137485B (en) | Materials and methods for efficient succinate and malate production |