DK2169076T3 - Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf - Google Patents
Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf Download PDFInfo
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- DK2169076T3 DK2169076T3 DK09171688.6T DK09171688T DK2169076T3 DK 2169076 T3 DK2169076 T3 DK 2169076T3 DK 09171688 T DK09171688 T DK 09171688T DK 2169076 T3 DK2169076 T3 DK 2169076T3
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Claims (16)
1. Isoleret, syntetisk eller rekombinant nukleinsyre omfattende: (A) en sekvens, der koder for et polypeptid med alpha-amylase-aktivitet, hvor sekvensen har mindst 97 %, 98 %, 99 % eller 100 % sekvensidentitet med sekvensen med SEQ ID NO: 125; eller (B) en nukleinsyre omfattende en sekvens, der koder for et polypeptid med alpha-amylase-aktivitet, hvor polypeptidets aminosyresekvens har mindst 97%, 98 %, 99 % eller 100 % sekvensidentitet med aminosyresekvensen med SEQ ID NO: 126; eller (C) en nukleinsyre omfattende en sekvens fra et hvilket som helst af (A) til (B), der koder for et polypeptid, der ikke har en signalsekvens; eller (D) en nukleinsyre omfattende: (i) en sekvens fra et hvilket som helst af (A) til (C), der koder for et polypeptid, yderligere omfattende en heterolog nukleinsyresekvens; (ii) sekvensen fra (i), hvor den heterologe nukleinsyresekvens koder for en signalsekvens; (iii) sekvensen fra (ii), hvor den heterologe signalsekvens omfatter en amylase-signalsekvens; eller (E) en nukleinsyresekvens, der er fuldstændigt komplementær til en hvilken som helst nukleinsyresekvens fra (A) til (D).
2. Fremgangsmåde til fremstilling af et rekombinant polypeptid; omfattende trinnene at indføre en nukleinsyre, der koder for polypeptidet, ind i en værtscelle under betingelser, der muliggør ekspression af polypeptidet, og at udvinde polypeptidet, hvor polypeptidet omfatter et polypeptid, der kodes af en nukleinsyre ifølge krav 1.
3. Nukleinsyresonde omfattende: (a) et oligonukleotid omfattende (i) mindst 35 på hinanden følgende nukleoti-der af nukleotidsekvensen med SEQ ID NO: 125 eller af en nukleotidse-kvens, der er fuldstændigt komplementær til sekvensen med SEQ ID NO: 125, hvor sekvensen hybridiserer til en nukleinsyre-target-region under meget stringente betingelser til dannelse af et detekterbart target:sonde-duplex; eller (ii) sekvensen fra (i), hvor de meget stringente betingelser omfatter en vask i 30 minutter ved rumtemperatur i en buffer omfattende 150 mM NaCI, 20 mM Tris-hydrochlorid, pH 7,8, 1 mM Na2EDTA, 0,5% SDS, efterfulgt af en 30 minutters vask i frisk buffer ved en temperatur, der er ca. 5 °C lavere end sekvensens smeltetemperatur (Tm); (b) sonden fra (a), hvor oligonukleotiden omfatter DNA eller RNA; (c) sonden fra (a) eller (b), yderligere omfattende en detekterbar isotopisk markering; eller (d) sonden fra (a), (b) eller (c), yderligere omfattende en detekterbar ikke-isotopisk markering udvalgt fra gruppen bestående af et fluorescerende molekyle, et kemiluminescerende molekyle, et enzym, en cofaktor, et enzymsubstrat og et hapten.
4. Vektor omfattende: (a) en nukleinsyre ifølge krav 1, eller sonden ifølge krav 3; eller (b) vektoren fra (a), hvor vektoren er en kloningsvektor eller en ekspressionsvektor.
5. Værtscelle omfattende (a) en nukleinsyre ifølge krav 1, eller sonden ifølge krav 3 eller vektoren ifølge krav 4; eller (b) værtscellen fra (a), hvor værtscellen er udvalgt fra gruppen bestående af: en prokaryotcelle, en eukaryotcelle, en svampecelle, en gærcelle og en plantecelle.
6. Isoleret, syntetisk eller rekombinant polypeptid med en alpha-amylase-aktivitet, omfattende: (a) en aminosyresekvens, der har mindst 97 %, 98 %, 99 % eller 100 % sekvensidentitet med aminosyresekvensen med SEQ ID NO: 126; (b) en aminosyresekvens, der kodes af en nukleinsyre ifølge krav 1; eller (c) aminosyresekvensen fra (a) eller (b), hvor polypeptidet danner antistoffer, som binder specifikt til et alpha-amylase-polypeptid; (d) aminosyresekvensen fra (a) til (c), hvor polypeptidet ikke har en signalsekvens; eller (e) (i) aminosyresekvensen fra (a) til (d), hvor polypeptidet yderligere omfatter en heterolog aminosyresekvens; (ii) sekvensen fra (i), hvor den heterologe aminosyresekvens omfatter en signalsekvens; (iii) sekvensen fra (ii), hvor den heterologe signalsekvens omfatter en amylase-signalsekvens; eller (f) aminosyresekvensen fra et hvilket som helst af (a) til (e), hvor polypeptidet omfatter en eller flere modifikationer og har mindst 97 %, 98 %, 99 % eller 100 % sekvensidentitet med aminosyresekvensen med SEQ ID NO: 126, og hvor modifikationen omfatter acetylering, acylering, ADP-ribosylering, amide-ring, kovalent tilknytning af flavin, kovalent tilknytning af en hæm-del, kovalent tilknytning af et nukleotid eller nukleotidderivat, kovalent tilknytning af et lipid eller lipidderivat, kovalent tilknytning af et phosphatidylinositol, tværbindende cyklisering, disulfidbindingsdannelse, demetylering, dannelse af kovalente tværbindinger, dannelse af cystein, dannelse af pyroglutamat, formyle-ring, gamma-carboxylering, glycosylering, GPI-ankerdannelse, hydroxylering, iodering, methylering, myristolyering, oxidering, pegylering, proteolytisk bearbejdning, phosphorylering, prenylering, racemisering, selenoylering eller sulfaten ng.
7. Fremgangsmåde til hydrolysering af en a-1,4-glycosidisk binding, omfattende: (A) at bringe en sammensætning med en a-1,4-glycosidisk binding i kontakt med et polypeptid, der kan hydrolysere en a-1,4-glycosidisk binding, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsy-resekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6, under betingelser, der letter hydrolyse af den a-1,4-glycosidiske binding; (B) fremgangsmåden fra (A), hvor sammensætningen omfatter stivelse, hvor stivelsen eventuelt er afledt fra ris, spiret ris, majs, byg, hvede, bælgfrugter, søde kartofler, milo, sorghum, rug, bulgur eller en kombination deraf; eller (C) fremgangsmåden fra (A) eller (B), yderligere omfattende tilsætning af et andet polypeptid, en alpha-amylase, en beta-amylase eller en kombination deraf.
8. Fremgangsmåde til at gøre en stivelsesomfattende sammensætning flydende (stivelsesforflydning), omfattende: (A) at bringe en stivelsesomfattende sammensætning i kontakt med et polypeptid med alpha-amylase-aktivitet under betingelser, der letter det at gøre stivelsen flydende (stivelsesforflydning), til dannelse af opløseligt stivelseshydrolysat, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsy-resekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; (B) fremgangsmåden fra (A), hvor stivelsen er afledt fra ris, spiret ris, majs, byg, hvede, bælgfrugter, søde kartofler, milo, sorghum, rug, bulgur eller en kombination deraf; (C) fremgangsmåden fra (A) eller (B), yderligere omfattende tilsætning af et andet polypeptid, en alpha-amylase, en beta-amylase eller en kombination deraf; (D) fremgangsmåden fra (A), (B) eller (C), yderligere omfattende saccharifi-cering af det opløselige stivelseshydrolysat.
9. Fremgangsmåde til behandling af lignocellulosiske fibre, omfattende (A) at bringe de lignocellulosiske fibre i kontakt med et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; eller (B) fremgangsmåden fra (A), hvor fremgangsmåden yderligere omfatter tilsætning af et andet polypeptid omfattende en amylase, en alpha-amylase eller en beta-amylase eller en kombination deraf.
10. Anvendelse af et polypeptid ifølge krav 6 i eller til en proces udvalgt fra gruppen bestående af fremstilling af brændstofethanol, en majs-vådformalingsproces, en bageproces eller proces til fremstilling af et brød eller et bagt produkt eller en proces til forhindring af, at et brød bliver for gammelt, en boringsproces, en brygningsproces, afsværtning af papir eller papirmasse, vask af en genstand, tekstilbehandling eller -afsletning, bearbejdning af papir eller papirmasse og fremstilling af en drikkevare.
11. Sammensætning omfattende (A) et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; (B) sammensætningen fra (A) yderligere omfattende et andet polypeptid omfattende tilsætning af et andet polypeptid, omfattende en amylase, en alpha-amylase, en beta-amylase eller en kombination deraf; (C) sammensætningen fra (A) eller (B), hvor sammensætningen er en fodersammensætning, papir, papirmasse eller genanvendt papir, et tekstil, et detergent eller et detergentadditiv, en bage-færdigblanding, et bage-tilsætningsstof, mel eller en dej; eller (D) fodersammensætningen fra (C), hvor fodersammensætningen omfatter ris, spiret ris, majs, byg, hvede, bælgfrugter, søde kartofler, milo, sorghum, rug, bulgur eller en kombination deraf.
12. Fremgangsmåde til fremstilling af et foder, omfattende (A) at bringe et foder i kontakt med et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; eller (B) fremgangsmåden fra (A), hvor fremgangsmåden yderligere omfatter anvendelse af et andet polypeptid omfattende en amylase, en alpha-amylase eller en beta-amylase eller en kombination deraf.
13. Fremgangsmåde til fremstilling af en alkohol, omfattende (A) at bringe en sammensætning med en a-1,4-glycosidisk binding i kontakt med et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; (B) fremgangsmåden fra (A), yderligere omfattende tilsætning af et andet polypeptid omfattende en amylase, en alpha-amylase, en beta-amylase eller en kombination deraf; eller (C) fremgangsmåden fra (A) eller (B), hvor alkoholen omfatter et brænd-stofethanol.
14. Majs-vådformalingsproces omfattende (A) anvendelse af et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; eller (B) fremgangsmåden fra (A), hvor processen yderligere omfatter anvendelse af et andet polypeptid omfattende en amylase, en alpha-amylase, en beta-amylase eller en kombination deraf.
15. Oliefelt-proces omfattende: (A) anvendelse af et polypeptid med alpha-amylase-aktivitet, hvor polypepti-det omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; (B) fremgangsmåden fra (A), hvor oliefelt-processen er boring; eller (C) fremgangsmåden fra (A) eller (B), hvor fremgangsmåden omfatter at øge strømmen af produktionsfluider fra en underjordisk formation ved fjernelse af en viskøs, stivelsesholdig fluid dannet under produktionsprocesser og fundet i den underjordiske formation, der omgiver en færdig brøndboring, omfattende (a) at lade produktionsfluider strømme fra brøndboringen; (b) at reducere strømmen af produktionsfluider fra den underjordiske formation til under de forventede flowrater; (a) tilvejebringe polypeptidet fra (A); (d) at pumpe polypeptidet til et ønsket sted i brøndboringen; og (e) at lade polypeptidet nedbryde den viskøse, stivelsesholdige fluid, hvor fluiden eventuelt fjernes fra den underjordiske formation til brøndoverfladen; (D) fremgangsmåden fra (A), (B) eller (C), hvor oliefelt-processen yderligere omfatter anvendelse af et andet polypeptid omfattende en amylase, en al-pha-amylase, en beta-amylase eller en kombination deraf.
16. Fremgangsmåde til fremstilling af sukker, omfattende (A) at bringe en sammensætning med en a-1,4-glycosidisk binding i kontakt med et polypeptid med alpha-amylase-aktivitet, hvor polypeptidet omfatter (a) en aminosyresekvens, der kodes af nukleinsyresekvensen ifølge krav 1, eller (b) en aminosyresekvens ifølge krav 6; eller (B) fremgangsmåden fra (A), hvor bearbejdningen af papir eller papirmasse yderligere omfatter anvendelse af et andet polypeptid omfattende en amylase, en alpha-amylase, en beta-amylase eller en kombination deraf.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US27049501P | 2001-02-21 | 2001-02-21 | |
US27049601P | 2001-02-21 | 2001-02-21 | |
US29112201P | 2001-05-14 | 2001-05-14 | |
EP02723192.7A EP1392815B1 (en) | 2001-02-21 | 2002-02-21 | Enzymes having alpha amylase activity and methods of use thereof |
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DK2169076T3 true DK2169076T3 (da) | 2017-02-06 |
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DK09171688.6T DK2169076T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf |
DK10184478.5T DK2333099T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf |
DK02706401.3T DK1370674T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alfa-amylase-aktivitet samt fremgangsmåder til anvendelse deraf |
DK10184381.1T DK2333097T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf |
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DK10184478.5T DK2333099T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf |
DK02706401.3T DK1370674T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alfa-amylase-aktivitet samt fremgangsmåder til anvendelse deraf |
DK10184381.1T DK2333097T3 (da) | 2001-02-21 | 2002-02-21 | Enzymer med alpha-amylase-aktivitet og fremgangsmåder til anvendelse deraf |
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US (10) | US7273740B2 (da) |
EP (8) | EP2333097B1 (da) |
JP (9) | JP2004533219A (da) |
CN (3) | CN101724640B (da) |
AT (1) | ATE510023T1 (da) |
AU (3) | AU2002240489C1 (da) |
BR (1) | BRPI0207770A8 (da) |
CA (6) | CA2438205C (da) |
CY (1) | CY1111771T1 (da) |
DK (4) | DK2169076T3 (da) |
ES (4) | ES2637501T3 (da) |
HK (1) | HK1060373A1 (da) |
MX (1) | MXPA03007527A (da) |
PT (1) | PT1370674E (da) |
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Families Citing this family (97)
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US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
DE60042730D1 (de) * | 1999-01-05 | 2009-09-24 | Univ Boston | Verbessertes klonierungsverfahren |
US20030017552A1 (en) * | 2000-07-21 | 2003-01-23 | Jarrell Kevin A. | Modular vector systems |
US7435562B2 (en) | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
US7659102B2 (en) | 2001-02-21 | 2010-02-09 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
US7560126B2 (en) | 2001-02-21 | 2009-07-14 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
EP2333097B1 (en) | 2001-02-21 | 2017-05-17 | BASF Enzymes LLC | Enzymes having alpha amylase activity and methods of use thereof |
DE10138753B4 (de) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen |
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2006
- 2006-04-06 US US11/400,030 patent/US7666633B2/en not_active Expired - Lifetime
- 2006-09-04 AU AU2006207843A patent/AU2006207843C1/en not_active Ceased
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2007
- 2007-01-09 US US11/621,534 patent/US7781201B2/en not_active Expired - Lifetime
- 2007-01-09 US US11/621,543 patent/US7816108B2/en not_active Expired - Lifetime
- 2007-01-09 US US11/621,528 patent/US7785855B2/en not_active Expired - Lifetime
- 2007-11-27 JP JP2007305644A patent/JP4690381B2/ja not_active Expired - Fee Related
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2008
- 2008-05-26 JP JP2008136876A patent/JP4846760B2/ja not_active Expired - Fee Related
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2009
- 2009-09-25 US US12/567,550 patent/US8334118B2/en not_active Expired - Fee Related
- 2009-09-25 AU AU2009222426A patent/AU2009222426B2/en not_active Ceased
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2010
- 2010-06-10 JP JP2010132602A patent/JP5576718B2/ja not_active Expired - Fee Related
- 2010-11-30 ZA ZA2010/08280A patent/ZA201008280B/en unknown
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2011
- 2011-02-04 JP JP2011022288A patent/JP5719621B2/ja not_active Expired - Lifetime
- 2011-08-18 CY CY20111100795T patent/CY1111771T1/el unknown
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2012
- 2012-11-06 US US13/669,707 patent/US20130224800A1/en not_active Abandoned
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2014
- 2014-08-05 JP JP2014159425A patent/JP6305866B2/ja not_active Expired - Fee Related
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2016
- 2016-04-22 US US15/135,732 patent/US10047350B2/en not_active Expired - Fee Related
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2017
- 2017-01-04 JP JP2017000091A patent/JP6402205B2/ja not_active Expired - Fee Related
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2018
- 2018-07-23 JP JP2018137584A patent/JP2019010100A/ja active Pending
- 2018-08-02 US US16/052,853 patent/US20180334660A1/en not_active Abandoned
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