DK21084A - Fremgangsmaade til fremstilling af et protein i en vaertscellekultur, dna-sekvens der koder for et dhfr-protein vektor for dna-sekvensen og vektor for denne og en anden dna-sekvens der koder for proteinet og selektering af transficerede eukaryotiske celler - Google Patents

Fremgangsmaade til fremstilling af et protein i en vaertscellekultur, dna-sekvens der koder for et dhfr-protein vektor for dna-sekvensen og vektor for denne og en anden dna-sekvens der koder for proteinet og selektering af transficerede eukaryotiske celler Download PDF

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Publication number
DK21084A
DK21084A DK21084A DK21084A DK21084A DK 21084 A DK21084 A DK 21084A DK 21084 A DK21084 A DK 21084A DK 21084 A DK21084 A DK 21084A DK 21084 A DK21084 A DK 21084A
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dna sequence
protein
vector
transfer
selecting
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DK21084A
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DK21084D0 (da
Inventor
Arthur David Levinson
Christian Clinton Simonsen
Elizabeth Mcleod Yelverton
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Genentech Inc
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Publication of DK21084A publication Critical patent/DK21084A/da

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00Reverse transcribing DNA viruses
    • C12N2730/00011Details
    • C12N2730/10011Hepadnaviridae
    • C12N2730/10111Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
DK21084A 1983-01-19 1984-01-18 Fremgangsmaade til fremstilling af et protein i en vaertscellekultur, dna-sekvens der koder for et dhfr-protein vektor for dna-sekvensen og vektor for denne og en anden dna-sekvens der koder for proteinet og selektering af transficerede eukaryotiske celler DK21084A (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45915183A 1983-01-19 1983-01-19

Publications (2)

Publication Number Publication Date
DK21084D0 DK21084D0 (da) 1984-01-18
DK21084A true DK21084A (da) 1984-08-24

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK21084A DK21084A (da) 1983-01-19 1984-01-18 Fremgangsmaade til fremstilling af et protein i en vaertscellekultur, dna-sekvens der koder for et dhfr-protein vektor for dna-sekvensen og vektor for denne og en anden dna-sekvens der koder for proteinet og selektering af transficerede eukaryotiske celler

Country Status (7)

Country Link
EP (1) EP0117060A3 (da)
JP (1) JPS59192089A (da)
KR (1) KR840007254A (da)
AU (1) AU2353384A (da)
DK (1) DK21084A (da)
ES (1) ES8600780A1 (da)
ZA (1) ZA84376B (da)

Cited By (1)

* Cited by examiner, † Cited by third party
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US3004784A (en) * 1959-07-20 1961-10-17 Tubular Structures Corp Of Ame Coupling pin

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