DK174933B1 - Modificeret hygromycin B-resistens DNA-sekvens samt plasmider, vektorer og transformanter indeholdende denne DNA-sekvens - Google Patents

Modificeret hygromycin B-resistens DNA-sekvens samt plasmider, vektorer og transformanter indeholdende denne DNA-sekvens Download PDF

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DK174933B1
DK174933B1 DK198403536A DK353684A DK174933B1 DK 174933 B1 DK174933 B1 DK 174933B1 DK 198403536 A DK198403536 A DK 198403536A DK 353684 A DK353684 A DK 353684A DK 174933 B1 DK174933 B1 DK 174933B1
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Prior art keywords
dna sequence
plasmids
vectors
well
transformants containing
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DK198403536A
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DK353684A (da
DK353684D0 (da
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Thomas Dominick Ingolia
Ramachandra Nagaraja Rao
Kevin Ray Kaster
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Syngenta Participations Ag
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/57581Thymosin; Related peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK198403536A 1983-07-22 1984-07-19 Modificeret hygromycin B-resistens DNA-sekvens samt plasmider, vektorer og transformanter indeholdende denne DNA-sekvens DK174933B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51622283A 1983-07-22 1983-07-22
US53550883A 1983-09-26 1983-09-26

Publications (3)

Publication Number Publication Date
DK353684D0 DK353684D0 (da) 1984-07-19
DK353684A DK353684A (da) 1985-01-23
DK174933B1 true DK174933B1 (da) 2004-03-01

Family

ID=27058764

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198403536A DK174933B1 (da) 1983-07-22 1984-07-19 Modificeret hygromycin B-resistens DNA-sekvens samt plasmider, vektorer og transformanter indeholdende denne DNA-sekvens

Country Status (10)

Country Link
EP (1) EP0135291B1 (da)
JP (1) JPH0712312B2 (da)
CA (1) CA1278540C (da)
DE (1) DE3481627D1 (da)
DK (1) DK174933B1 (da)
FI (1) FI842878A (da)
GB (1) GB2143534B (da)
GR (1) GR79980B (da)
IE (1) IE57776B1 (da)
IL (1) IL72426A (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340766C (en) * 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
RU1780542C (ru) * 1985-04-22 1992-12-07 Эли Лилли Энд Компани Способ конструировани рекомбинантной плазмидной ДНК pPS 20, кодирующей изопенициллин-N-синтетазу, способ получени штамма СернаLоSроRIUм асRемоNIUм, обладающего активностью изопенициллин-N-синтетазы
CA1309673C (en) * 1985-04-22 1992-11-03 Stephen Wyatt Queener Recombinant dna expression vectors and dna compounds which encode isopenicillin n synthetase
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
US6774283B1 (en) 1985-07-29 2004-08-10 Calgene Llc Molecular farming
DE4410188A1 (de) * 1994-03-24 1995-09-28 Boehringer Mannheim Gmbh Gentherapeutisches Verfahren unter Verwendung von antibiotikaresistenzgenfreien DNA-Vektoren

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422685A1 (fr) * 1978-04-14 1979-11-09 Pasteur Institut Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli
FR2428075A1 (fr) * 1978-06-08 1980-01-04 Pasteur Institut Procede de production de proteines par expression des genes correspondants dans des bacteries et vecteurs susceptibles d'etre mis en oeuvre dans de tels procedes
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
JPS5813598A (ja) * 1981-06-22 1983-01-26 イーライ・リリー・アンド・カンパニー 組み替えdnaクロ−ニング・ベクタ−ならびにその真核細胞性および原核細胞性被形質転換体
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof

Also Published As

Publication number Publication date
FI842878A (fi) 1985-01-23
AU3089884A (en) 1985-01-24
GR79980B (da) 1984-10-31
JPS6047685A (ja) 1985-03-15
GB2143534B (en) 1987-07-29
DK353684A (da) 1985-01-23
DE3481627D1 (de) 1990-04-19
GB2143534A (en) 1985-02-13
EP0135291B1 (en) 1990-03-14
IE57776B1 (en) 1993-04-07
CA1278540C (en) 1991-01-02
IL72426A0 (en) 1984-11-30
IE841847L (en) 1985-01-22
DK353684D0 (da) 1984-07-19
FI842878A0 (fi) 1984-07-17
IL72426A (en) 1990-03-19
EP0135291A1 (en) 1985-03-27
JPH0712312B2 (ja) 1995-02-15
AU565625B2 (en) 1987-09-24
GB8418171D0 (en) 1984-08-22

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