DK200300821A - Fremgangsmåde til in vitro molekylær evolution af proteinfunktion - Google Patents
Fremgangsmåde til in vitro molekylær evolution af proteinfunktion Download PDFInfo
- Publication number
- DK200300821A DK200300821A DK200300821A DKPA200300821A DK200300821A DK 200300821 A DK200300821 A DK 200300821A DK 200300821 A DK200300821 A DK 200300821A DK PA200300821 A DKPA200300821 A DK PA200300821A DK 200300821 A DK200300821 A DK 200300821A
- Authority
- DK
- Denmark
- Prior art keywords
- single stranded
- fragments
- exonuclease
- sequences
- polynucleotide
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract description 22
- 238000000338 in vitro Methods 0.000 title 1
- 230000004853 protein function Effects 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 abstract description 25
- 102000040430 polynucleotide Human genes 0.000 abstract description 25
- 239000002157 polynucleotide Substances 0.000 abstract description 25
- 239000012634 fragment Substances 0.000 abstract description 12
- 108060002716 Exonuclease Proteins 0.000 abstract description 8
- 102000013165 exonuclease Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 238000000137 annealing Methods 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 abstract description 2
- 102000053602 DNA Human genes 0.000 abstract description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract description 2
- 229920001184 polypeptide Polymers 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 description 1
- 108010046914 Exodeoxyribonuclease V Proteins 0.000 description 1
- 102100029075 Exonuclease 1 Human genes 0.000 description 1
- 102000019236 Exonuclease V Human genes 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0030252A GB2370038B (en) | 2000-12-12 | 2000-12-12 | A method for in vitro molecular evolution of protein function |
| US09/734,801 US6958213B2 (en) | 2000-12-12 | 2000-12-12 | Method for in vitro molecular evolution of protein function |
| PCT/EP2001/014744 WO2002048351A2 (en) | 2000-12-12 | 2001-12-12 | A method for in vitro molecular evolution of protein function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK200300821A true DK200300821A (da) | 2003-08-12 |
Family
ID=26245418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200300821A DK200300821A (da) | 2000-12-12 | 2003-06-03 | Fremgangsmåde til in vitro molekylær evolution af proteinfunktion |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1341909B2 (enExample) |
| JP (1) | JP4430303B2 (enExample) |
| AT (1) | ATE295882T1 (enExample) |
| AU (2) | AU2002234572B2 (enExample) |
| CA (1) | CA2430582C (enExample) |
| DE (1) | DE60110926T3 (enExample) |
| DK (1) | DK200300821A (enExample) |
| ES (1) | ES2244670T5 (enExample) |
| PT (1) | PT1341909E (enExample) |
| WO (1) | WO2002048351A2 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| US20030036641A1 (en) * | 2001-01-31 | 2003-02-20 | Padgett Hal S. | Methods for homology-driven reassembly of nucleic acid sequences |
| US7262012B2 (en) | 2002-05-17 | 2007-08-28 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations |
| WO2004016811A2 (en) * | 2002-08-19 | 2004-02-26 | Danmarks Tekniske Universitet (Dtu) | Methods and kit for genes shuffling using tagged primers |
| GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| GB0607798D0 (en) | 2006-04-20 | 2006-05-31 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0708376D0 (en) | 2007-05-01 | 2007-06-06 | Alligator Bioscience Ab | Novel polypeptides and uses thereof |
| US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| GB0905790D0 (en) | 2009-04-03 | 2009-05-20 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0920258D0 (en) | 2009-11-19 | 2010-01-06 | Alligator Bioscience Ab | New medical agents and use thereof |
| GB201115280D0 (en) | 2011-09-05 | 2011-10-19 | Alligator Bioscience Ab | Antibodies, uses and methods |
| GB201311487D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Bispecific molecules |
| GB201311475D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Polypeptides |
| EP3552665A3 (en) | 2014-08-12 | 2019-12-18 | Alligator Bioscience AB | Antibodies |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5851804A (en) * | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
| GB9712512D0 (en) * | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| WO2000072013A1 (en) * | 1999-05-21 | 2000-11-30 | The Penn State Research Foundation | Construction of incremental truncation libraries |
-
2001
- 2001-12-12 JP JP2002550068A patent/JP4430303B2/ja not_active Expired - Fee Related
- 2001-12-12 PT PT01985402T patent/PT1341909E/pt unknown
- 2001-12-12 CA CA2430582A patent/CA2430582C/en not_active Expired - Fee Related
- 2001-12-12 AT AT01985402T patent/ATE295882T1/de active
- 2001-12-12 AU AU2002234572A patent/AU2002234572B2/en not_active Ceased
- 2001-12-12 AU AU3457202A patent/AU3457202A/xx active Pending
- 2001-12-12 ES ES01985402T patent/ES2244670T5/es not_active Expired - Lifetime
- 2001-12-12 WO PCT/EP2001/014744 patent/WO2002048351A2/en not_active Ceased
- 2001-12-12 DE DE60110926T patent/DE60110926T3/de not_active Expired - Lifetime
- 2001-12-12 EP EP01985402A patent/EP1341909B2/en not_active Expired - Lifetime
-
2003
- 2003-06-03 DK DK200300821A patent/DK200300821A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DE60110926T2 (de) | 2006-03-23 |
| JP4430303B2 (ja) | 2010-03-10 |
| ES2244670T3 (es) | 2005-12-16 |
| ES2244670T5 (es) | 2012-08-02 |
| EP1341909A2 (en) | 2003-09-10 |
| AU2002234572B2 (en) | 2006-07-06 |
| WO2002048351A2 (en) | 2002-06-20 |
| ATE295882T1 (de) | 2005-06-15 |
| CA2430582C (en) | 2012-12-04 |
| EP1341909B1 (en) | 2005-05-18 |
| DE60110926D1 (de) | 2005-06-23 |
| DE60110926T3 (de) | 2012-06-14 |
| CA2430582A1 (en) | 2002-06-20 |
| EP1341909B8 (en) | 2006-05-03 |
| EP1341909B2 (en) | 2012-04-11 |
| WO2002048351A3 (en) | 2003-06-05 |
| AU3457202A (en) | 2002-06-24 |
| JP2004515248A (ja) | 2004-05-27 |
| PT1341909E (pt) | 2005-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PHB | Application deemed withdrawn due to non-payment or other reasons |
Effective date: 20150204 |