DE60110926T3 - Eine methode zur in vitro molekularen evolution der proteinfunktion - Google Patents

Eine methode zur in vitro molekularen evolution der proteinfunktion Download PDF

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Publication number
DE60110926T3
DE60110926T3 DE60110926T DE60110926T DE60110926T3 DE 60110926 T3 DE60110926 T3 DE 60110926T3 DE 60110926 T DE60110926 T DE 60110926T DE 60110926 T DE60110926 T DE 60110926T DE 60110926 T3 DE60110926 T3 DE 60110926T3
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Germany
Prior art keywords
fragments
dna
exonuclease
polynucleotide
lane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60110926T
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German (de)
English (en)
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DE60110926T2 (de
DE60110926D1 (de
Inventor
Roland Carlsson
Christina Furebring
Ann-Christin Malmborg Hager
Carl Borrebaeck
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Alligator Bioscience AB
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Alligator Bioscience AB
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Priority claimed from GB0030252A external-priority patent/GB2370038B/en
Priority claimed from US09/734,801 external-priority patent/US6958213B2/en
Application filed by Alligator Bioscience AB filed Critical Alligator Bioscience AB
Publication of DE60110926D1 publication Critical patent/DE60110926D1/de
Publication of DE60110926T2 publication Critical patent/DE60110926T2/de
Application granted granted Critical
Publication of DE60110926T3 publication Critical patent/DE60110926T3/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60110926T 2000-12-12 2001-12-12 Eine methode zur in vitro molekularen evolution der proteinfunktion Expired - Lifetime DE60110926T3 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US734801 2000-12-12
GB0030252A GB2370038B (en) 2000-12-12 2000-12-12 A method for in vitro molecular evolution of protein function
US09/734,801 US6958213B2 (en) 2000-12-12 2000-12-12 Method for in vitro molecular evolution of protein function
GB0030252 2000-12-12
EP01985402A EP1341909B2 (en) 2000-12-12 2001-12-12 A method for in vitro molecular evolution of protein function
PCT/EP2001/014744 WO2002048351A2 (en) 2000-12-12 2001-12-12 A method for in vitro molecular evolution of protein function

Publications (3)

Publication Number Publication Date
DE60110926D1 DE60110926D1 (de) 2005-06-23
DE60110926T2 DE60110926T2 (de) 2006-03-23
DE60110926T3 true DE60110926T3 (de) 2012-06-14

Family

ID=26245418

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60110926T Expired - Lifetime DE60110926T3 (de) 2000-12-12 2001-12-12 Eine methode zur in vitro molekularen evolution der proteinfunktion

Country Status (10)

Country Link
EP (1) EP1341909B2 (enExample)
JP (1) JP4430303B2 (enExample)
AT (1) ATE295882T1 (enExample)
AU (2) AU2002234572B2 (enExample)
CA (1) CA2430582C (enExample)
DE (1) DE60110926T3 (enExample)
DK (1) DK200300821A (enExample)
ES (1) ES2244670T5 (enExample)
PT (1) PT1341909E (enExample)
WO (1) WO2002048351A2 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20030036641A1 (en) * 2001-01-31 2003-02-20 Padgett Hal S. Methods for homology-driven reassembly of nucleic acid sequences
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
WO2004016811A2 (en) * 2002-08-19 2004-02-26 Danmarks Tekniske Universitet (Dtu) Methods and kit for genes shuffling using tagged primers
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0607798D0 (en) 2006-04-20 2006-05-31 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0708376D0 (en) 2007-05-01 2007-06-06 Alligator Bioscience Ab Novel polypeptides and uses thereof
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
GB0905790D0 (en) 2009-04-03 2009-05-20 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0920258D0 (en) 2009-11-19 2010-01-06 Alligator Bioscience Ab New medical agents and use thereof
GB201115280D0 (en) 2011-09-05 2011-10-19 Alligator Bioscience Ab Antibodies, uses and methods
GB201311487D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Bispecific molecules
GB201311475D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Polypeptides
EP3552665A3 (en) 2014-08-12 2019-12-18 Alligator Bioscience AB Antibodies

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
WO2000072013A1 (en) * 1999-05-21 2000-11-30 The Penn State Research Foundation Construction of incremental truncation libraries

Also Published As

Publication number Publication date
DE60110926T2 (de) 2006-03-23
JP4430303B2 (ja) 2010-03-10
ES2244670T3 (es) 2005-12-16
ES2244670T5 (es) 2012-08-02
DK200300821A (da) 2003-08-12
EP1341909A2 (en) 2003-09-10
AU2002234572B2 (en) 2006-07-06
WO2002048351A2 (en) 2002-06-20
ATE295882T1 (de) 2005-06-15
CA2430582C (en) 2012-12-04
EP1341909B1 (en) 2005-05-18
DE60110926D1 (de) 2005-06-23
CA2430582A1 (en) 2002-06-20
EP1341909B8 (en) 2006-05-03
EP1341909B2 (en) 2012-04-11
WO2002048351A3 (en) 2003-06-05
AU3457202A (en) 2002-06-24
JP2004515248A (ja) 2004-05-27
PT1341909E (pt) 2005-10-31

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