DK175429B1 - Fremgangsmåde til immunbestemmelse med reduceret uspecifikke forstyrrelser og diagnostisk middel til anvendelse ved fremgangsmåden - Google Patents
Fremgangsmåde til immunbestemmelse med reduceret uspecifikke forstyrrelser og diagnostisk middel til anvendelse ved fremgangsmåden Download PDFInfo
- Publication number
- DK175429B1 DK175429B1 DK198900966A DK96689A DK175429B1 DK 175429 B1 DK175429 B1 DK 175429B1 DK 198900966 A DK198900966 A DK 198900966A DK 96689 A DK96689 A DK 96689A DK 175429 B1 DK175429 B1 DK 175429B1
- Authority
- DK
- Denmark
- Prior art keywords
- igg
- specific
- aggregates
- reactants
- mak33
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000000032 diagnostic agent Substances 0.000 title claims description 14
- 229940039227 diagnostic agent Drugs 0.000 title claims description 14
- 238000003018 immunoassay Methods 0.000 title description 14
- 239000000376 reactant Substances 0.000 claims abstract description 50
- 239000000126 substance Substances 0.000 claims abstract description 25
- 230000027455 binding Effects 0.000 claims abstract description 22
- 238000009739 binding Methods 0.000 claims abstract description 22
- 241000282414 Homo sapiens Species 0.000 claims abstract description 18
- 239000012634 fragment Substances 0.000 claims description 21
- 239000000427 antigen Substances 0.000 claims description 18
- 102000036639 antigens Human genes 0.000 claims description 17
- 108091007433 antigens Proteins 0.000 claims description 17
- 230000000694 effects Effects 0.000 claims description 17
- 241001465754 Metazoa Species 0.000 claims description 15
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 241000894007 species Species 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 229920002521 macromolecule Polymers 0.000 claims description 6
- 239000013060 biological fluid Substances 0.000 claims description 4
- 239000003112 inhibitor Substances 0.000 claims description 4
- 101100129256 Mus musculus Mak gene Proteins 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 229920001519 homopolymer Polymers 0.000 claims description 3
- 101100290050 Rattus norvegicus Mak gene Proteins 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 claims description 2
- 108700021042 biotin binding protein Proteins 0.000 claims description 2
- 102000043871 biotin binding protein Human genes 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000012928 buffer substance Substances 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 41
- 210000002966 serum Anatomy 0.000 abstract description 21
- 230000001900 immune effect Effects 0.000 abstract description 6
- 230000002452 interceptive effect Effects 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 74
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 36
- 239000000872 buffer Substances 0.000 description 29
- 238000012360 testing method Methods 0.000 description 25
- 239000000203 mixture Substances 0.000 description 24
- 238000002360 preparation method Methods 0.000 description 24
- 241000699666 Mus <mouse, genus> Species 0.000 description 21
- 239000008363 phosphate buffer Substances 0.000 description 21
- 235000018102 proteins Nutrition 0.000 description 19
- 102000004169 proteins and genes Human genes 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 19
- 239000000523 sample Substances 0.000 description 19
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 18
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 18
- 239000011780 sodium chloride Substances 0.000 description 18
- 238000011534 incubation Methods 0.000 description 17
- 102100033806 Alpha-protein kinase 3 Human genes 0.000 description 15
- 231100000743 Maximale Arbeitsplatzkonzentration Toxicity 0.000 description 15
- 238000004132 cross linking Methods 0.000 description 15
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- 238000007792 addition Methods 0.000 description 12
- 241000283690 Bos taurus Species 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 10
- 238000003556 assay Methods 0.000 description 10
- 230000008033 biological extinction Effects 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 108090001008 Avidin Proteins 0.000 description 8
- 108010090804 Streptavidin Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 108010088751 Albumins Proteins 0.000 description 6
- 102000009027 Albumins Human genes 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 108060003951 Immunoglobulin Proteins 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- 102000018358 immunoglobulin Human genes 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 4
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 4
- 206010029719 Nonspecific reaction Diseases 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000011088 calibration curve Methods 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 4
- 235000018417 cysteine Nutrition 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 108091006905 Human Serum Albumin Proteins 0.000 description 3
- 102000008100 Human Serum Albumin Human genes 0.000 description 3
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 229960002086 dextran Drugs 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 229920000140 heteropolymer Polymers 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- SEEYREPSKCQBBF-UHFFFAOYSA-N n-methylmaleimide Chemical compound CN1C(=O)C=CC1=O SEEYREPSKCQBBF-UHFFFAOYSA-N 0.000 description 3
- 239000000123 paper Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000008057 potassium phosphate buffer Substances 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- KUWPCJHYPSUOFW-SCWFEDMQSA-N (2r,3r,4s,5r)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)OC1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-SCWFEDMQSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000008064 anhydrides Chemical class 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 230000006287 biotinylation Effects 0.000 description 2
- 238000007413 biotinylation Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 238000005470 impregnation Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 229960003742 phenol Drugs 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000007430 reference method Methods 0.000 description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 description 2
- 239000012279 sodium borohydride Substances 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 239000004071 soot Substances 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 description 1
- SNUSZUYTMHKCPM-UHFFFAOYSA-N 1-hydroxypyridin-2-one Chemical compound ON1C=CC=CC1=O SNUSZUYTMHKCPM-UHFFFAOYSA-N 0.000 description 1
- FZWBNHMXJMCXLU-UHFFFAOYSA-N 2,3,4,5-tetrahydroxy-6-[3,4,5-trihydroxy-6-[[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxyhexanal Chemical compound OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OCC(O)C(O)C(O)C(O)C=O)O1 FZWBNHMXJMCXLU-UHFFFAOYSA-N 0.000 description 1
- AWFYPPSBLUWMFQ-UHFFFAOYSA-N 2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]-1-(1,4,6,7-tetrahydropyrazolo[4,3-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NN=C(O1)CC(=O)N1CC2=C(CC1)NN=C2 AWFYPPSBLUWMFQ-UHFFFAOYSA-N 0.000 description 1
- WDTLSHLXZWPLNU-UHFFFAOYSA-N 2-amino-3-(2-iodoacetyl)benzoic acid Chemical compound NC1=C(C(O)=O)C=CC=C1C(=O)CI WDTLSHLXZWPLNU-UHFFFAOYSA-N 0.000 description 1
- KALVLKKPGPBOKG-UHFFFAOYSA-N 2-aminohexanoic acid 1-hydroxypyrrolidine-2,5-dione Chemical compound ON1C(=O)CCC1=O.CCCCC(N)C(O)=O KALVLKKPGPBOKG-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 230000010777 Disulfide Reduction Effects 0.000 description 1
- 102000008857 Ferritin Human genes 0.000 description 1
- 108050000784 Ferritin Proteins 0.000 description 1
- 238000008416 Ferritin Methods 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 102000003946 Prolactin Human genes 0.000 description 1
- 108010057464 Prolactin Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- MJOQJPYNENPSSS-XQHKEYJVSA-N [(3r,4s,5r,6s)-4,5,6-triacetyloxyoxan-3-yl] acetate Chemical compound CC(=O)O[C@@H]1CO[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O MJOQJPYNENPSSS-XQHKEYJVSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 238000012801 analytical assay Methods 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 210000001742 aqueous humor Anatomy 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- SNCZNSNPXMPCGN-UHFFFAOYSA-N butanediamide Chemical compound NC(=O)CCC(N)=O SNCZNSNPXMPCGN-UHFFFAOYSA-N 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 150000001720 carbohydrates Chemical group 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- VXIVSQZSERGHQP-UHFFFAOYSA-N chloroacetamide Chemical compound NC(=O)CCl VXIVSQZSERGHQP-UHFFFAOYSA-N 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 229960003624 creatine Drugs 0.000 description 1
- 239000006046 creatine Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- FRTGEIHSCHXMTI-UHFFFAOYSA-N dimethyl octanediimidate Chemical compound COC(=N)CCCCCCC(=N)OC FRTGEIHSCHXMTI-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- ZWIBGKZDAWNIFC-UHFFFAOYSA-N disuccinimidyl suberate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCCC(=O)ON1C(=O)CCC1=O ZWIBGKZDAWNIFC-UHFFFAOYSA-N 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 210000001156 gastric mucosa Anatomy 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000008240 homogeneous mixture Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000000677 immunologic agent Substances 0.000 description 1
- 229940124541 immunological agent Drugs 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940097325 prolactin Drugs 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 238000010405 reoxidation reaction Methods 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 1
- 239000001433 sodium tartrate Substances 0.000 description 1
- 229960002167 sodium tartrate Drugs 0.000 description 1
- 235000011004 sodium tartrates Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrotherapy Devices (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Medicinal Preparation (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/164,054 US4914040A (en) | 1988-03-03 | 1988-03-03 | Reagent and method for determination of a polyvalent substance using an immunoaggregate |
| US16405488 | 1988-03-03 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK96689D0 DK96689D0 (da) | 1989-02-28 |
| DK96689A DK96689A (da) | 1989-09-04 |
| DK175429B1 true DK175429B1 (da) | 2004-10-18 |
Family
ID=22592781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK198900966A DK175429B1 (da) | 1988-03-03 | 1989-02-28 | Fremgangsmåde til immunbestemmelse med reduceret uspecifikke forstyrrelser og diagnostisk middel til anvendelse ved fremgangsmåden |
Country Status (18)
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3730059A1 (de) * | 1987-09-08 | 1989-03-30 | Behringwerke Ag | Verfahren zur bestimmung von "loeslichem" fibrin |
| US5141853A (en) * | 1988-07-22 | 1992-08-25 | Abbott Laboratories | Determination of ammonia levels in a sample |
| DE3829245A1 (de) * | 1988-08-29 | 1990-03-01 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
| WO1991006559A1 (en) * | 1989-10-24 | 1991-05-16 | E.I. Du Pont De Nemours And Company | Use of polymerized immunoglobulin to reduce the incidence of false-positive responses in immunoassays |
| US6274325B1 (en) | 1990-06-25 | 2001-08-14 | Boehringer Mannheim Gmbh | Method for carrying out a homogeneous-immunoassay based on agglutination |
| DE4020204A1 (de) * | 1990-06-25 | 1992-01-02 | Boehringer Mannheim Gmbh | Durchfuehrung eines homogenen immunoassays nach dem agglutinationsprinzip |
| JPH04350559A (ja) * | 1991-05-28 | 1992-12-04 | Dai Ichi Pure Chem Co Ltd | 特異抗体の測定法 |
| EP0597902B1 (en) * | 1991-07-26 | 1997-03-05 | Dade Chemistry Systems Inc. | Method for eliminating erroneous results in an immunoassay |
| US5460944A (en) * | 1991-10-28 | 1995-10-24 | Boehringer Mannheim Gmbh | Storable protein solution |
| DE4202923A1 (de) * | 1992-02-01 | 1993-08-05 | Behringwerke Ag | Verfahren zur bestimmung von antigenen oder antikoerpern in gegenwart eines immunkomplexes |
| IL102495A (en) * | 1992-07-14 | 1998-06-15 | Patchornik Avraham | Universal standard reagents, method of preparing same and use thereof |
| IL106887A (en) * | 1993-09-02 | 1997-02-18 | Yissum Res Dev Co | Pharmaceutical and diagnostic compositions and a method for drug targeting |
| GB9403215D0 (en) * | 1994-02-19 | 1994-04-13 | Kodak Ltd | Chemical modification of antibodies and antigens |
| DE4407423A1 (de) * | 1994-03-05 | 1995-09-07 | Boehringer Mannheim Gmbh | Entstörmittel zum Einsatz bei Immunoassays |
| WO1995023801A1 (de) * | 1994-03-05 | 1995-09-08 | Boehringer Mannheim Gmbh | Entstörmittel zum einsatz bei immunoassays |
| DE4439452A1 (de) * | 1994-11-04 | 1996-05-09 | Boehringer Mannheim Gmbh | Antikörperklassenspezifisches Entstörungsreagenz |
| DE19621133A1 (de) * | 1996-05-24 | 1997-11-27 | Boehringer Mannheim Gmbh | Bestimmungsverfahren mit oligomerisierten Rezeptoren |
| DE19731465A1 (de) | 1997-07-22 | 1999-01-28 | Boehringer Mannheim Gmbh | Verwendung von Kontrollflächen zur Detektion von Störproben in einem Nachweisverfahren |
| ES2185104T3 (es) * | 1997-12-11 | 2003-04-16 | Roche Diagnostics Gmbh | Eliminacionde las interferencias en procedimientos de diagnostico mediante peptidos de d-aminoacidos. |
| DE19828466A1 (de) * | 1998-06-26 | 1999-12-30 | Roche Diagnostics Gmbh | Entstörung von Immunoassays durch Substanzen, die aus den Framework-Regionen von Antikörpern abgeleitet sind |
| US6406858B1 (en) * | 1998-11-27 | 2002-06-18 | Bayer Corporation | System for the reduction of interferences in immunoassays |
| WO2002027316A2 (en) * | 2000-09-25 | 2002-04-04 | Abbott Laboratories | Methods and kits for decreasing interferences of assays samples containing plasma or serum in specific binding assays by using a large polycation |
| DE10059720A1 (de) | 2000-11-30 | 2002-06-06 | Roche Diagnostics Gmbh | Verwendung intramolekular kovalent vernetzter Proteine als Bindepartner in Immunoassays |
| DE10064827A1 (de) | 2000-12-22 | 2002-06-27 | Dade Behring Marburg Gmbh | Nachweisverfahren |
| US20040091952A1 (en) * | 2002-07-25 | 2004-05-13 | Sysmex Corporation | Reagent kit for detecting lupus anticoagulant |
| US20040018556A1 (en) * | 2002-07-29 | 2004-01-29 | Cantor Thomas L. | Reagent and method for determination of a substance using an immunoaggregator |
| EP1469317B1 (en) * | 2003-03-28 | 2008-10-08 | Sysmex Corporation | Reagent for detecting anti-phospholipid antibody |
| CN1849514A (zh) * | 2003-07-08 | 2006-10-18 | 因韦尔尼斯医药瑞士股份有限公司 | 颗粒凝集的检测方法和装置 |
| US20050112586A1 (en) * | 2003-11-24 | 2005-05-26 | Roland Janzen | Method and composition for stabilizing liquid reagents |
| ATE395603T1 (de) * | 2004-02-06 | 2008-05-15 | Sysmex Corp | Reagenziensatz zum nachweis von lupus- antikoagulant. |
| CN1969189B (zh) * | 2004-06-14 | 2012-03-21 | 协和梅迪克斯株式会社 | 非特异性反应被抑制的可溶性白介素-2受体免疫学测定方法和试剂 |
| CN101120249A (zh) * | 2004-11-15 | 2008-02-06 | 因韦尔尼斯医药瑞士股份有限公司 | 血型方法系统以及装置 |
| US7172906B2 (en) * | 2004-11-16 | 2007-02-06 | Dade Behring Inc. | Reduction of non-specific binding in assays |
| US20110189704A1 (en) * | 2007-08-23 | 2011-08-04 | Mitsubishi Chemical Medience Corporation | Non-specific reaction inhibitor |
| US8956859B1 (en) | 2010-08-13 | 2015-02-17 | Aviex Technologies Llc | Compositions and methods for determining successful immunization by one or more vaccines |
| WO2019207115A1 (en) * | 2018-04-26 | 2019-10-31 | Statens Serum Institut | Improved immunoassays |
| CN109342743B (zh) * | 2018-12-05 | 2022-02-18 | 菲鹏生物股份有限公司 | 一种能够与类风湿因子高效结合的变性IgG的制备方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0083869B1 (en) * | 1982-01-05 | 1985-10-16 | International Institute Of Cellular And Molecular Pathology (Icp) | Method of immunoassay |
| DE3206729A1 (de) * | 1982-02-25 | 1983-09-01 | Behringwerke Ag, 3550 Marburg | Immunologisches agglutinationsverfahren |
| DE3225027A1 (de) * | 1982-07-05 | 1984-01-05 | Boehringer Mannheim Gmbh, 6800 Mannheim | Immunchemisches messverfahren |
| US4659678A (en) * | 1982-09-29 | 1987-04-21 | Serono Diagnostics Limited | Immunoassay of antigens |
| US4474892A (en) * | 1983-02-16 | 1984-10-02 | Board Of Trustees Of The Leland Stanford Junior University | Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same |
| US4582810A (en) * | 1983-09-30 | 1986-04-15 | Becton, Dickinson And Company | Immuno-agglutination particle suspensions |
| JPS60256057A (ja) * | 1984-06-01 | 1985-12-17 | Dai Ichi Pure Chem Co Ltd | 免疫学的測定法 |
| JPS6165162A (ja) * | 1984-09-05 | 1986-04-03 | Chemo Sero Therapeut Res Inst | モノクロ−ナル抗体を用いた免疫学的測定方法における非特異反応吸収剤 |
| JPH0799372B2 (ja) * | 1985-07-13 | 1995-10-25 | 富士レビオ株式会社 | 逆受身凝集反応用非特異反応吸収剤 |
-
1988
- 1988-03-03 US US07/164,054 patent/US4914040A/en not_active Expired - Lifetime
-
1989
- 1989-02-23 DE DE3905505A patent/DE3905505A1/de not_active Withdrawn
- 1989-02-27 DE DE8989103388T patent/DE58902586D1/de not_active Expired - Lifetime
- 1989-02-27 EP EP89103388A patent/EP0331068B1/de not_active Expired - Lifetime
- 1989-02-27 ES ES89103388T patent/ES2045216T3/es not_active Expired - Lifetime
- 1989-02-27 AT AT89103388T patent/ATE82072T1/de not_active IP Right Cessation
- 1989-02-28 DD DD89326088A patent/DD280396A5/de not_active IP Right Cessation
- 1989-02-28 DK DK198900966A patent/DK175429B1/da not_active IP Right Cessation
- 1989-03-01 ZA ZA891568A patent/ZA891568B/xx unknown
- 1989-03-01 AU AU30908/89A patent/AU616484B2/en not_active Expired
- 1989-03-02 PT PT89889A patent/PT89889B/pt not_active IP Right Cessation
- 1989-03-02 FI FI891012A patent/FI92879C/fi not_active IP Right Cessation
- 1989-03-02 RU SU894613606A patent/RU2032906C1/ru active
- 1989-03-02 HU HU891015A patent/HU212322B/hu unknown
- 1989-03-02 IE IE68389A patent/IE62974B1/en not_active IP Right Cessation
- 1989-03-03 JP JP1050230A patent/JPH0823560B2/ja not_active Expired - Lifetime
- 1989-03-03 CA CA000592675A patent/CA1340572C/en not_active Expired - Lifetime
-
1993
- 1993-02-05 GR GR920402739T patent/GR3006991T3/el unknown
- 1993-06-02 LV LVP-93-465A patent/LV10538B/lv unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2045216T3 (es) | 1994-01-16 |
| JPH0823560B2 (ja) | 1996-03-06 |
| DK96689A (da) | 1989-09-04 |
| IE890683L (en) | 1989-09-03 |
| DE58902586D1 (de) | 1992-12-10 |
| CA1340572C (en) | 1999-06-01 |
| DD280396A5 (de) | 1990-07-04 |
| AU616484B2 (en) | 1991-10-31 |
| US4914040A (en) | 1990-04-03 |
| EP0331068A1 (de) | 1989-09-06 |
| LV10538B (en) | 1995-12-20 |
| FI92879C (fi) | 1995-01-10 |
| AU3090889A (en) | 1989-09-07 |
| PT89889A (pt) | 1989-11-10 |
| FI92879B (fi) | 1994-09-30 |
| DK96689D0 (da) | 1989-02-28 |
| HUT50988A (en) | 1990-03-28 |
| LV10538A (lv) | 1995-02-20 |
| RU2032906C1 (ru) | 1995-04-10 |
| IE62974B1 (en) | 1995-03-08 |
| ATE82072T1 (de) | 1992-11-15 |
| HU212322B (en) | 1996-05-28 |
| EP0331068B1 (de) | 1992-11-04 |
| JPH01254869A (ja) | 1989-10-11 |
| PT89889B (pt) | 1994-05-31 |
| GR3006991T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1993-06-30 |
| FI891012A0 (fi) | 1989-03-02 |
| FI891012L (fi) | 1989-09-04 |
| ZA891568B (en) | 1989-11-29 |
| DE3905505A1 (de) | 1989-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK175429B1 (da) | Fremgangsmåde til immunbestemmelse med reduceret uspecifikke forstyrrelser og diagnostisk middel til anvendelse ved fremgangsmåden | |
| Wisdom | Enzyme-immunoassay. | |
| Yorde et al. | Competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. I. Measurement of human choriogonadotropin. | |
| Yolken | Enzyme-linked immunosorbent assay (ELISA): a practical tool for rapid diagnosis of viruses and other infectious agents | |
| US4650751A (en) | Protected binding assay avoiding non-specific protein interference | |
| EP0440044A1 (en) | Avoidance of human anti-mouse antibody interference in in vitro diagnostic testing | |
| EP0223843A4 (en) | METHOD AND ELEMENT FOR DETECTING IMMUNE COMPLEXES. | |
| US20020187510A1 (en) | Method for assay of antibodies and antibody assay device | |
| US4621048A (en) | Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy | |
| Merry et al. | A quantitative antiglobulin test for IgG for use in blood transfusion serology | |
| AU5317886A (en) | Solid phase diffusion assay | |
| US4929543A (en) | Process for the determination of an antibody in human body fluids | |
| CN115184620B (zh) | 一种pla2r抗体的量子点荧光检测试纸条、试剂盒及其应用 | |
| Tuson et al. | A novel immunohistochemical technique for demonstration of specific binding of human monoclonal antibodies to human cryostat tissue sections. | |
| Antes et al. | Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies | |
| Spitalnik et al. | A new technique in quantitative immunohematology: solid-phase kinetic enzyme-linked immunosorbent assay | |
| EP0324969A2 (en) | Method of measuring transferrin | |
| Grenner | Principles and Applications of Heterogeneous Enzyme Immunoassays | |
| KR910004228B1 (ko) | 면역집합물을 사용한 다가성분의 측정방법 | |
| Lown et al. | Evaluation of an enzyme-linked antiglobulin test for the detection of red cell antibodies | |
| Marsden et al. | Purification of mouse haptoglobin by antibody affinity chromatography and development of an ELISA to measure serum haptoglobin levels | |
| Akman et al. | The development and validation of a competitive, microtiter plate enzymeimmunoassay for human albumin in urine | |
| Harats et al. | IgG rheumatoid factor in purified IgG fractions and whole sera from patients with rheumatoid arthritis | |
| Russell et al. | Detection of food antigen-specific IgA immune complexes in human sera | |
| WO1986007152A1 (en) | Method and article for detection of immune complexes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUP | Patent expired |