DK173985A - Blodkoagulationsinhiberende polypeptider, deres fremstilling eller udvinding, samt anvendelse deraf som diagnostika og reagenser - Google Patents

Blodkoagulationsinhiberende polypeptider, deres fremstilling eller udvinding, samt anvendelse deraf som diagnostika og reagenser Download PDF

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Publication number
DK173985A
DK173985A DK173985A DK173985A DK173985A DK 173985 A DK173985 A DK 173985A DK 173985 A DK173985 A DK 173985A DK 173985 A DK173985 A DK 173985A DK 173985 A DK173985 A DK 173985A
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diagnostics
reagents
recovery
preparation
blood coagulation
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DK173985A
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DK173985D0 (da
DK171824B1 (da
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Dominique Tripier
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Hoechst Ag
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/26—Containing cys-cys disulfide bridge between nonadjacent cysteine residues

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

hver gang 2 af de 6 Cys-grupper i positionerne 5, 13, 15, 21, 27 og 38 er forbundet via disulfid-broer, samt dets fysiologisk acceptable salte, idet polypeptidet med formlen I, hvori Η-(X) -Y er H-Val-Val og n = 0, er undtaget, fremstilles ved, at man a) fremstiller det på i og for sig kendt måde ved hjælp af fastfasesyntese, eller b) til fremstilling af et polypeptid, hvori η = 0, I. underkaster hirudin en Edman-nedbrydning to gange, II. omsætter det således tilvejebragte peptid med en aktiv ester af aminosyre eller et peptid med formlen U-(X)m-Y-0H, hvori U er en urethanbeskyttelsesgruppe, III. fraspalter phenylthio-carbamoyl-gruppen ved e-aminofunktionen af Lys ved hjælp af hydrazin, IV. og urethanbeskyttelsesgruppen U ved hjælp af en syre eller en base, og eventuelt overfører det ved a) eller b) tilvejebragte polypeptid i dets fysiologisk acceptable salt. Polypeptidet kan også udvindes fra orme af Gnathobdellida-ordenen, fortrinsvis fra orme af Hirudo--slægten ved hjælp af en kombination af ekstraktionsmetoder, fældningsmetoder, membranfiltrering og/eller chroma-tografiske fremgangsmåder, og det tilvejebragte peptid kan eventuelt overføres i dets fysiologisk acceptable salte.
Det af blodigler udvundne polypeptid har aminosyresammen-sætningen 9 Asparaginsyre, 5 Threonin, 4 Serin, 13 Gluta-minsyre, 3 Prolin, 9 Glycin, 2 Valin, 6 Cystein, 2 Iso-leucin, 4 Leucin, 2 Tyrosin, 1 Phenylalanin, 3 Lysin og 1 Histidin, hvori OH-gruppen i en af tyrosingrupperne kan være forestret med svovlsyre.
Polypeptiderne er blodkoagulationsinhibitorer og kan anvendes som diagnostika og reagenser.
DK173985A 1984-04-18 1985-04-17 Blodkoagulationsinhiberende hirudinderivater, fremgangsmåde til deres udvinding, deres anvendelse samt middel indeholdende dem DK171824B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3414593 1984-04-18
DE3414593 1984-04-18
DE3438296 1984-10-19
DE19843438296 DE3438296A1 (de) 1984-04-18 1984-10-19 Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel

Publications (3)

Publication Number Publication Date
DK173985D0 DK173985D0 (da) 1985-04-17
DK173985A true DK173985A (da) 1985-10-19
DK171824B1 DK171824B1 (da) 1997-06-23

Family

ID=25820510

Family Applications (1)

Application Number Title Priority Date Filing Date
DK173985A DK171824B1 (da) 1984-04-18 1985-04-17 Blodkoagulationsinhiberende hirudinderivater, fremgangsmåde til deres udvinding, deres anvendelse samt middel indeholdende dem

Country Status (18)

Country Link
US (1) US4668662A (da)
EP (1) EP0158986B1 (da)
JP (1) JP2552263B2 (da)
KR (1) KR930000054B1 (da)
AR (1) AR242800A1 (da)
AU (1) AU580295B2 (da)
CA (1) CA1271298A (da)
DE (2) DE3438296A1 (da)
DK (1) DK171824B1 (da)
ES (3) ES8605772A1 (da)
FI (1) FI851521L (da)
GR (1) GR850942B (da)
HU (1) HU198085B (da)
IE (1) IE60581B1 (da)
IL (1) IL74948A (da)
NO (1) NO851535L (da)
PT (1) PT80301B (da)
ZA (1) ZA852854B (da)

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DE3506992A1 (de) * 1985-02-27 1986-08-28 Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
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US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
NZ228995A (en) * 1988-05-10 1992-03-26 Merrell Dow Pharma Hirudin peptide derivatives and pharmaceutical compositions
US4971953A (en) * 1988-05-10 1990-11-20 Merrell Dow Pharmaceuticals Anticoagulant peptide alcohols
DE68919848T2 (de) * 1988-05-27 1995-05-04 Philadelphia Children Hospital Amphiphile peptide und deren verwendung.
US5114921A (en) * 1988-05-27 1992-05-19 The Children's Hospital Of Philadelphia Amphiphilic peptides and use thereof
DE3819078A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Amblyommin, ein neuer wirkstoff fuer die antikoagulationstherapie
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
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DE3939801A1 (de) * 1989-12-01 1991-06-06 Basf Ag Neue proteine und ihre herstellung
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DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
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Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341414C (fr) * 1984-03-27 2002-12-31 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens

Also Published As

Publication number Publication date
FI851521L (fi) 1985-10-19
KR850007801A (ko) 1985-12-09
ES542310A0 (es) 1985-12-16
AR242800A1 (es) 1993-05-31
IE60581B1 (en) 1994-07-27
PT80301B (de) 1987-02-10
ES542311A0 (es) 1985-12-16
EP0158986A3 (en) 1988-09-21
ZA852854B (en) 1985-11-27
JPS60233098A (ja) 1985-11-19
KR930000054B1 (ko) 1993-01-06
HUT37806A (en) 1986-02-28
AU4136485A (en) 1985-10-24
FI851521A0 (fi) 1985-04-16
DE3438296A1 (de) 1985-11-07
IL74948A (en) 1989-09-10
HU198085B (en) 1989-07-28
AU580295B2 (en) 1989-01-12
CA1271298A (en) 1990-07-03
NO851535L (no) 1985-10-21
PT80301A (de) 1985-05-01
US4668662A (en) 1987-05-26
JP2552263B2 (ja) 1996-11-06
GR850942B (da) 1985-11-25
DK173985D0 (da) 1985-04-17
IE850983L (en) 1985-10-18
ES8605770A1 (es) 1985-12-16
DE3587576D1 (de) 1993-10-21
ES8605771A1 (es) 1985-12-16
IL74948A0 (en) 1985-08-30
EP0158986B1 (de) 1993-09-15
ES542309A0 (es) 1985-12-16
DK171824B1 (da) 1997-06-23
ES8605772A1 (es) 1985-12-16
EP0158986A2 (de) 1985-10-23

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