AU628473B2 - Process for renaturing incorrect recombinants of insulin precursors - Google Patents

Description

6z 8473.

COMMONWEALTH OF AUSTRALIA PATENTS ACT 1952-69 Form COMPLETE SPECIFICATION

(ORIGINAL)

Class Int. Class Application Number: Lodlged: Complete Specification Lodged: Accepted; *s Published: ::"P~riority :lated Art Name of Applicant: HOEe2HST AKTIENGESELLSCHAFP 64Adress of Applicant 50 Bruningstrasse, D-6230 Frankfurt/Main 80, Federal Republic of Germany Actual inventor: MICHAEL DORSCHJG A!Idress for Service WATERMARK PATENT TRADEMARK ATTORNEYS.

29TTo Australiahor~ff Complete Specification for the invention entitled: PROCESS FOR RENATURING INCORRECT RECOMBINANTS OF INSULIN PRECURSORS The following statemyent Is a full description of this Invention, Including the best method of performing It known to J rsrsrC HOECHST AKTIENGESELLSCHAFT HOE 89/F 020 Dr.ME/rh Description Process for renaturing incorrect recombinants of insulin precursors Insulir is a molecule which consists of 2 polypeptide chains linked to one another via disulfide bridges. The A chain consists of 21 amino acids and the B chain of amino acids. These two chains are linked to one another in the precursor molecule, the proinsulin, by a peptide, the C-peptide. The C-peptide in human proinsulin consists of 35 amino acids. In the context of the maturation process of the hormnone, the C-peptide is split off by specific proteases and the proinsulin is thus converted into insulin (Davidson et al., Nature 333, 93-96, 1988).

.15 In addition to the naturally occurring C-peptides, a large number of joining possibilities between the A chain and B chain are described in the literature (Yanaihara et Soo: al., Diabetes 27, 149-160 (1978), Busse et al., Biochemistry 15, 1649-1657 (1971), and Geiger et al., Biochem. Biophys. Res. Com. 55, 60-66 (1973)).

s In the context of genetic engineering, it is now possible to prepare insulin from microorganisms modified by genetic engineering. If E. coli is used as the microorganism, the product is frequently expressed as fusion protein, that is to say the product is coupled with a protein endogenous to the bacteria, for example with 3- S galactosidase. This fusion protein precipitates out in the cell and is in this way protected from proteolytic degradation. After breakdown of the cell, the fusion protein content is split off chemically or enzymatically and the 6 cysteines of the insulin precursor are converted into their S-sulfonates (-S-S0 3 by means of oxidative sulfitolysis. In a subsequent ("renaturing or recombination") step, natural preproinsulin must be produced from this so-called preproinsulin S-sulflnate by formation of the 3 correct disulfide bridges that is to

I--

1 i rP-rc-n Sr 2 say bridges from A6 to All, from A7 to B7 and from to B19 in the corresponding insulin peptide sequences.

According to the process described in EP-B-0,037,255, this step is carried out, for example, by reaction of the starting S-sulfonate with a mercaptan in an amount which results in 1 to 5 SH radicals per SS0 3 radical in an aqueous medium at a pH of 7 to 11.5 and an S-sulfonate concentration of up to 10 mg per ml of aqueous medium, preferably in the absence of an oxidizing agent.

Yields of in some cases more than 80% are said to be obtained here.

e SIn addition to the (desired) renaturing products with correct disulfide bridges, more or less substantial *'15 amounts of (undesired) "incorrect" recombinants, that is 0:00 to say insulin products with disulfide bridges which are only partly correct or not correct at all and also with intermolecular disulfide bridges, are always also formed depending on the reaction conditions and in particular 20 the concentration circumstances in this and practically all other known renaturing or recombination processes in which insulin precursors with opened disulfide bridges are converted into products with the correspondingly closed disulfide bridges.

5 When the insulin precursor products obtained by the known renaturing processes are worked up to insulin without the "incorrect" recombinants being removed this working up being effected by known techniques (chemically or enzymatically) no (natural) insulin is formed from the "incorrect" recombinants.

It is therefore advantageous or necessary to remove the "incorrect" recombinants from the renaturing products with the correct disulfide bridges before working up the corresponding renaturing products to give insulin. This 3 3 can be effected, for example, by known chromatographic processes. In another particularly advantageous process, the removal is effected by adjusting the reaction mixture to pH 4 to 6 preferably in the presence of a small amount of a physiologically acceptable surface-active substance the "correct" recombinants remaining virtually completely in solution and the "incorrect" recombinants being precipitated DE-A-35 01 641).

The "incorrect" recombinants removed are then advantageously converted back by sulfitolysis into the corresponding S-sulfonate, which is subjected to renewed folding, it often being necessary to remove by-products formed during the sulfitolysis by chromatography before the renaturing. The "incorrect" recombinants can in this way largely be converted back into "usable" product.

Sulfitolysis of the "incorrect" recombinants with subsequent chromatography and renewed folding of course means a not inconsiderable expenditure.

In the efforts to avoid or at least reduce this expendi- 0'20 ture, it has now been found that this is possible by reacting the "incorrect" recombinants of insulin precursors with excess mercaptan ii an aqueous medium in the .I presence of an organic redox system or at least one organic compound which forms such an organic redox system under the reaction conditions.

The "incorrect" recombinants can in this way be converted in high yields directly into the "correct' renaturing products or "correct" recombinants without ,,lfitolysis, which represents a considerable advantage compared with the prior art.

There is also no prior art at all which would have made obvious in any manner bypassing of sulfitolysis with the subsequent folding stage merely by reaction with excess mercaptan and the addition of an organic redox system.

i 4 Possible "incorrect" recombinants of insulin precursors for the process according to the invention are preferably the products which are formed as by-products on recombination of insulin precursors with opened bridges of the following formula I: Gly-NH

I

Cys-S-R3 S-R 3 I 33 I I (A-20) (A-21) Cys Z R 2 I (A-11) I

S-H

3

S-R

S-R

3

S-R,

0000 00 0 0* 0 o 1) I

R

1 (B-19) (Bin which RI H or an amino acid or peptide radical which can be split off chemically or enzymatically,

R

2 OH or an amino acid or peptide radical, preferably OH,

R

3 H or a Cys-S protective group, preferably the -SO, 3 or the tert.-butyl group, X a radical which joins the insulin A and B chains, preferably an amino acid or peptide radical, Y the radical of a genetically encodable amino acid, preferably Thr, Ala or Ser, in particular Thr, Z the radical of a genetically encodable amino acid, preferably Asn, Gln, Asp, Glu, Gly, Ser, Thr, Ala or Met, in particular Asn, and A1-A20 and B1-B29 peptide sequences of insulin which are non-mutated or mutated by replacement of one or more amino acids, C-CIIIC-----~eLL--UIIICC ~IIII~I~ 5 preferably the non-mutated peptide sequences of human, porcine or bovine insulin, in particular of human or porcine insulin.

If RI H in formula I, the products are products which are derived from proinsulin; if R 1 an amino acid or peptide radical which can be split off chemically o:c enzymatically, the products are products which are derived from preproinsulin.

Amino acid radicals which can be split off chemically are those which are split off, for example, by means of BrCN or N-bromosuccinimide; these are, for example, methionine (Met) or tryptophan (Trp).

0 Amino acid radicals which can be split off enzymatically are those which can be split off, for example, by means l5 of trypsin (such as, for example, Arg or Lys).

0*SO 0011 Peptide radicals which can be split off chemically or enzymatically are peptide radicals having at least 2 amino acids.

sea* All the amino acids possible for R, are preferably those of the group of naturally occurring amino acids, that is to say mainly Gly, Ala, Ser, Thr, Val, Lev, Ile, Asn, 0X1i, Cys Met, Tyr, Phe, Pro, Hyp, lrg, Lys, Hyl, Orn, Cit and His.

0

R

2 is OH or similarly to R, likewise an amino acid or peptide radical, the meaning of OH being preferred. The amino acids (including those which form the peptide radical consisting of at least 2 amino acid radicals) preferably originate as for R, from the group of naturally occurring amino acids.

R, is hydrogen or a cysteine-sulfur protective group, the

-SO

3 or the tert.-butyl group being preferred cysteinesulfur protective groups.

_I -Y Ti slsll~3asrar~;~ (8PIAlgPIIL~Ij I 6 X is a radical which joins the insulin A and B chains, preferably an amino acid or peptide radical.

If X is an amino acid radical, the radical of Arg or Lys is preferred; if X is a peptide radical, the radical of a naturally occurring C-peptide in particular the human, porcine or bovine insulin C-peptide is preferred.

Genetically encodable amino acids for Y are (in each case in the L form): Gly, Ala, Ser, Thr, Val, Leu, Ile, Asp, Asn, Glu, Gln, Cys, Met, Arg, Lys, His, Tyr, Phe, Trp and Pro.

a*O* Preferred genetically encodable amino acids are Thr, Ala and Ser, in particular Thr.

*fal Z can like Y elso denote the radical of a genetically encodable amino acid, but in this case Asn, Gln, Asp, Glu, Gly, Ser, Thr, Ala and Met, in particular Asn, are preferred.

ea J Al A20 and Bl B29 can in principle be the peptide sequences which are non-mutated or mutated by replacement of one or more amino acids and originate from all possible insulins; the mutants can be produced by known processes of genetic engineering (site directed mutagenesis). However, the non-mutated peptide sequences of human, porcine or bovine insulin, in particular of human 25 or porcine insulin (the Al A20 and Bl B29 sequences of human and porcine insulin are identical) are preferred.

The "incorrect" recombinants formed as by-products during recombination of insulin precursors with opened -S-Sbridges of the formula I are removed from the "correct" recombinants in a known manner preferably by precipitation at pH values of 4 to 6 in accordance with the process of the abovementioned DE-A-35 01 641 and are LIIIIIICUI I 7 then dissolved directly, or after prior freeze-drying, in water or an aqueous solution for use for the process according to the invention.

The concentration of the "incorrect" recombinants in the aqueous starting solution can vary within a wide range; preferred concentrations are about 0.1 to about 100 mg, in particular about 0.1 to about 10 mg/ml, the mg values relating to the "incorrect" recombinants as dried solid.

Suitable mercaptans for the reaction according to the invention are in principle all the possible organic compounds with SH groups; mercaptoethanol, thioglycolic acid, dithioerrthritol, glutathione and cysteine, in particular mercaptoethanol and cysteine, are preferred.

The mercaptans can be employed individually or as a mixture.

0* The amount of mercaptan to be employed can vary within wide limits; an excess of mercaptan corresponding to a ratio of mercaptan-SH groups/cysteine-S units (in the "incorrect" recombinants) of at least about 5 is preferred. This ratio has an upper limit imposed practically only by economic considerations. An upper limit of about 100 is advantageous.

Because of the wide range of variation of the excess mercaptan, the number of cysteine-S units in the "in- 25 correct" recombinant employed does not have to be determined completely accurately.

er.d-esa aa~AI6.Xorganic redox systems are pairs of compounds, one component of which is an organic compound having the structural element of the formula II OH OH 0

(II)

0 OH OH C C C L -t 8 or an aromatic o- or p-dihydroxy compound and the other component of which is an organic compound having the structural element of the formula II in oxidized form structural element of the formula II' 0 0 0 C- (II') or is an o- or p-quinone.

The free valencies of the structural element of the formula II and II' can be satisfied by hydrogen or organic groups, such as, for example, CI-C 4 -alkyl groups.

10 However, the structural element can also be part of a ring having preferably 4, 5 or 6 carbon ring atoms and if appropriate also one or two hetero atoms, such as, for example, 0, it being possible for the ring in turn to be substituted by groups which are inert under the reaction 15 conditions, such as, for example, alkyl or hydroxyalkyl groups.

Examples of compounds having the structural element of the formula II are: reductone OH OH 0 I 11 S- C C C

H

reductic acid i

I

OH OH

HC=====CH

H

2 C C

CH

methylreductic acid OH OH c 0 H CH3 ~---~llllplCIIICIILleLI-- ~-LIIIICC7' L uaa 9 ascorbic acid OH OH (vitamin C) OH HC CH OH HC CH HOCH CH CH C O=0 0 The formulae here are in each case written only in one of the tautomeric forms.

All the compounds are reducing. In the oxidized form, the structural element of the formula II becomes that of the formula II'.

Possible aromatic o- and p-dihydroxy compounds are in principle all the possible aromatic compounds having two OH groups in the o- or p-position, it merely being *necessary that the o- or p-quinone formation from the oand p-dihydroxy compounds cannot be prevented by any particular substituents or the like. Examaples of aromatic e o- and p-dihydroxy compounds are 1,2-dihydroxybenzene pyrocatechol, 1,4-dihdyroxybenzene hydroquinone, methyl,-hydroquinone, naphtho-l,4-hydroxyquinone and anthra.,hydroquinone; on oxidation, the corresponding quinoneb are f'oned therefrom.

S 0 In the reaction according to the invention the particular organic redox systems consisting, for example, of the pairs of compounds of ascorbic acid dehydroascorbic acid, pyrocatechol o-quinone, hydroquinone p-quinone, naphthohydroquinone naphthoquinone and the like, can thus be employed in virtually any desired ratio (preferably in an approximately equimolar ratio). However, it is also possible for only the particular individual components ,f these pairs of compounds that is to say, for example, only ascorbic acid or only dehydroascorbic acid or only hydroquinone and the like to be added, because the other particular component belonging to the redox pair of compounds (dehydroascorbic acid or ascorbic acid or p-quinone and the like) forms in the reaction medium.

Preferred organic redox systems are the combinations consisting of the pairs of compounds ascorbic acid dehydroascorbic acid, pyrocatechol o-quinone and hydroquinone p-quinone and preferred individual compounds which form such a redox system under the reaction conditions are the individual components of these pairs of compounds.

Ascorbic acio- and/or dehydroascorbic acid are especially preferred.

The amount employed of the compound(s) which form(s) the organic redox system can vary within wide limits. The number of mol of compound(s) which form(s) the organic redox system can be chosen as being between about i 15 1/10,000 and 10,000, preferably between about 1/10 and 10, based on one gram equivalent of mercaptan molecular weight of the mercaptan employed in g/n- aiber of SH groups in the mercaptan molecule).

It is advantageous also to add urea to the reaction solution, concentrations corresponding to about 0.1 to 1 M (M molar), in particular 0.1 0.5 M, being preferred.

The reaction according to the invention is advantageously SI*. carried out in the alkaline pH range, preferably between about 7 and 12, in particular between about 9.5 and 11.

STo maintain the desired pH, it is advantageous to add a buffer substance, the nature and ionic strength of the buffer having a certain influence on the folding yield.

It is advantageous to keep the ionic strength low, a range of about 1 mM (mM millimolar) to I M (M molar), in particular one from about 5 mM to 50 mM, being preferred. Buffer substances which can be used are, for example, borate buffer, carbonate buffer or glycine buffer, the latter being preferred.

Y -raP~ 1 3 ~~~l~lrr 78P6~b IIC--BLIIX~-X

I

11 A general range of reaction temperature which may be stated is one between about 0 and 45°C; a range from about 4 to 8 0 C is preferred.

Covering the renaturing solution with a layer of certain gases, such as, for example, oxygen, nitrogen o lXium, has no noticeable influence on the renaturing yield.

The reaction time is in general between about 2 and 24 hours, preferably between about 5 and 16 hours.

The renaturing product of the reaction according to the 10 invention is if the "incorrect" starting recombinant originates from recombination of an insulin precursor with opened S-S bridges of the abovementioned formula I an insulin precursor having correct disulfide bonds ("correct" recombinant) of the formula III Gly-N- X Cys-S-S I (A-20) (A-21) A-7) Cys Z R 2 (A-I (I11) S S S S (B-1)

R

1

Y

0. (B-19) in which R 1

R

2 X, Y and Z have the same meaning as in formula I.

When the reaction has ended (which can be aecertained, for example, by high performance liquid thromatography), the mixture is worked up in a known manner.

The "correctly" folded product, preferably of the formula III, can then be converted into the corresponding insulin enzymatically or chemically by known techniques.

I _II_ ~slrmar~rrr~r~----- 12 The following example is intended to illustrate the invention in more detail. Before the (invention) example, the preparation of the starting substance is also described by way of example.

A) Preparation of the starting substance 1. Folding of "miniproinsulin" 0c 15 .u9.

SS S e0 "Miniproinsulin-S-S03-", that is to say an insulin precursor in which the A and B chains ot the insulin are linked via an arginine and the B chain is lengthened N-te.rminally, is employed. The freeze-dried material (60% pure) is dissolved at a solids concentration of 0.5 g/l in 50 mM glycine buffer, pH 10.7, which corresponds to a precursor concentration of 0.3 g/l. 630 ml of 1 M mercaptoethanol and 630 ml of 1 M ascorbic acid are added to the batch (100 1), and the mixture is then stirred slowly in a cold chamber at 8°C for 16 hours. The folding yield, determined by high performance liquid chromatography against a standard, is 0.228 g/l (76% of theory).

2. Precipitation of aggregates ("incorrect" recombinants) 1 g of polyethylene-polypropylene glycol is added to the folding batch and the total batch is divided into batches of 20 ml, with which a pH precipitation series between pH 5.0 and pH 7.0 is set up in 0.5 pH value steps. After establishing the pH values, the mixtures are left to stand at room temperature for minutes and the precipitates are then centrifuged off.

The pernatants are quantified by means of high performance liquid chromatography in order to determine the precipitation losses, and the precipitates are combined and freeze-dried (weight: 15 g of eIid, content 40% pure).

Loss of correctly folded product as a function of the pH: r I--r -~L~P I If

I

13 pH 5.0 0% pH 5.5 6% pH 6.0 pH 6.5 9% pH 7.0 4% This series shows that the optim m precipitation pH is B) Example according to the invention' Renaturing g of freeze-dried precipitate are taken up in 5 1 of 4 M urea. 13.2 ml of mercaptoethanol (14.35 M) are added (final concentration about 35 mM) and the mixture is left r~t room temperature for 10 minutes. The 5 1 of solution are introduced into 23 1 of 50 mM glycine buffer, 188 ml of 1 M ascorbic acid are added and the pH is brought to 10.7. The batch is then stirred gently at 8°C for hours. The folding yield is 0.146 g/l (73% of theory).

The course of the reaction is monitored byr means of high performance liquid chromatography.

The total yield of the "miniproinsulin" folding Al) can thus be increased from 76% of theory to about 85% if theory.

10 *0*e 5

S

0* 5 0 so.

*005s5

S

Claims (19)

1. A process for renaturing "incorrect" recombinants of an insulin precujrsor, which comprises reacting the "incorrect" recombinant with excess mercaptan in an aqueous medium in the presence of an organic redox system being a pair of compounds, one compound of which is an organic compound having the structural element of the formula II OH OH 0 C- C- (II) 0 OH OH II II II C- C- or an aromatic o- or p-dihydroxy compound and the other component of which is an organic compound having the structural element of the formula II in oxidized form structural element of the formula II' 0 0 0 II II II (II') S- C C C or is an o- or p-quinone, or at least one organic compound which Iorms such an organic redox system under the reaction conditions. 'i

2. The process as claimed in claim 1, wherein the "incorrect" recombinants of an nsulin precursor used are the products which are formed as by-products on recombination of an Insulin precursor with open -S-S bridges of the formula I Gly-NH1 X II Cys-S-Ra S-R

3 I I (A-20) (A-21) Z R2 (A-11) (I) S-Ra S-R3 S-R3 S-R3 (B-i) R 1 -HN-Phe--Cys---- Y (B-19) In which Ri H or an amino acid or peptide radical which can be split off chemically or enzymatically, R 2 OH or an amino acid or peptide radical, R 3 H or a Cys-S protective group, X a radical which joins the insulin A and B chains, Y the radical of a genetically encodable amino acid, *Z the radical of a genetically encodable amino acid, A1-A20 and B1-B29 peptide sequences of insulin which are non-mutated or mutated by replacement of one or more amino acids. S 3. The process as claimed in either of claims 1 and 2, wherein the reaction is carried out at concentrations of the "incorrect" recombinants of about 0.1 to about 100 mg/ml.

4. The process as claimed in any one of claims 1 to 3, wherein mercaptoethanol and/or cysteine is used as the mercaptan.

The process as claimed in any one of claims 1 to 4, wherein the reaction Is carried out with an excess of mercaptan corresponding to a ratio of mercaptan-SH groups/cysteine- S units (in the "Incorrect" recombinants) of at least about r i i i ~l~Pl~s~ -t -I 16

6. The process as claimed in any one of claims 1 to 5, wherein the organic compounds employed which form an organic redox system under the reaction conditions are one or more of the ii.dividual components mentioned in claim 1.

7. The process as claimed in any one of claims 1 to 6, wherein the organic redox system employed is the pair of compounds ascorbic acid dehydroascorbic acid, pyrocatechol o-quinone or hydroquinone p-quinone and the organic compound employed which can form such a redox system under the reaction conditions is in each case only one component of this pair of compounds.

8. The process as claimed in any one of claims 1 to 6, wherein the reaction is carried out in the presence of ascorbic acid and/or dehydroascorbic Lcid.

9. The process as claimed in any one of claims 1 to 8, wherein the mercaptan and the compound(s) which form(s) the organic redox system are employed in a ratio of 1 gram equivalent of mercaptan to 1/10,000 to 10,000 mol of the compound(s) which form(s) the organic redox system.

The process as claimed in any one of claims 1 to 9, wherein the aqueous reaction medium contains dissolved urea of about 0.1 to 1.0 molar concentration.

11. The process as claimed in any one of claims 1 to 10, wherein the reaction is carried out at a pH of between about 7 and 12.

12. A process as claimed in ,nyone of claims 1 to 11 in which R 2 is OH the Cys-S protecting group of R 3 is the -SO" or the tert-butyl group the X radical is an amino acid or peptide radical Y is Thr, Ala or Ser Z Is Asn, Gin, Asp, Glu, Gly, Ser, Thr, Ala or Met. Sb o- I A J. 17

13. A process as claimed in claim 12 in which Y and Z are Asn.

14. A process as claimed in any one of claims 1 to 13 in which the peptide sequences of insulin are non-mutated sequences of human, porcine or bovine insulin.

A process as claimed in claim 14 in which the insulin is human or porcine insulin.

16. A process as claimed in claim 3 in which the concentration of the "incorrect" recombinants is about 0.1 to 10 mg/ml.

17. A process as claimed in claim 5 in which the ratio is about 5 to 100.

18. A process as claimed in claim 9 in which the ratio is 1/10 to

19. A process as claimed in claim 10 wherein the dissolved urea is in a concentration of :i about 0.1 to 0,5 molar. A process as claimed in claim 11 in which the pH is about 9,5 to 11 DATED this 19th day of June 1992 HOECHST AKTIENGESELLSCHAFT *WATERMARK PATENT TRADEMARK ATTORNEYS THE ATRIUM 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRAUA DOC 008 AU4862090.WPC DBM/IAS/BS/JJC