DK172064B1 - Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren - Google Patents

Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren Download PDF

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Publication number
DK172064B1
DK172064B1 DK568586A DK568586A DK172064B1 DK 172064 B1 DK172064 B1 DK 172064B1 DK 568586 A DK568586 A DK 568586A DK 568586 A DK568586 A DK 568586A DK 172064 B1 DK172064 B1 DK 172064B1
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DK
Denmark
Prior art keywords
fusion protein
plasmid
amino acids
gene structure
amino acid
Prior art date
Application number
DK568586A
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Danish (da)
English (en)
Other versions
DK568586D0 (da
DK568586A (da
Inventor
Paul Habermann
Friedrich Wengenmayer
Original Assignee
Hoechst Ag
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Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of DK568586D0 publication Critical patent/DK568586D0/da
Publication of DK568586A publication Critical patent/DK568586A/da
Application granted granted Critical
Publication of DK172064B1 publication Critical patent/DK172064B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DK568586A 1985-11-27 1986-11-26 Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren DK172064B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853541856 DE3541856A1 (de) 1985-11-27 1985-11-27 Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
DE3541856 1985-11-27

Publications (3)

Publication Number Publication Date
DK568586D0 DK568586D0 (da) 1986-11-26
DK568586A DK568586A (da) 1987-05-28
DK172064B1 true DK172064B1 (da) 1997-10-06

Family

ID=6286938

Family Applications (2)

Application Number Title Priority Date Filing Date
DK568586A DK172064B1 (da) 1985-11-27 1986-11-26 Fusionsprotein, dets fremstilling og anvendelse, genstruktur, som koder for fusionsproteinet, vektor, som indeholder genstrukturen, og værtscelle, som indeholder vektoren
DK052292A DK172210B1 (da) 1985-11-27 1992-04-21 Hirudinderivater

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK052292A DK172210B1 (da) 1985-11-27 1992-04-21 Hirudinderivater

Country Status (17)

Country Link
EP (3) EP0227938B1 (fr)
JP (1) JP2566933B2 (fr)
KR (1) KR950000300B1 (fr)
AT (2) ATE127841T1 (fr)
AU (1) AU595262B2 (fr)
CA (1) CA1341203C (fr)
DE (4) DE3541856A1 (fr)
DK (2) DK172064B1 (fr)
ES (3) ES2077747T3 (fr)
FI (1) FI93471C (fr)
GR (1) GR3005042T3 (fr)
HU (1) HU203579B (fr)
IE (1) IE59488B1 (fr)
IL (1) IL80755A0 (fr)
NO (1) NO176481C (fr)
PT (1) PT83813B (fr)
ZA (1) ZA868943B (fr)

Families Citing this family (35)

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Publication number Priority date Publication date Assignee Title
US3125021A (en) * 1955-11-14 1964-03-17 Smooth
EP0168342B1 (fr) * 1984-06-14 1991-07-03 Ciba-Geigy Ag Procédé pour la préparation d'inhibiteurs de thrombine
DE3712985A1 (de) * 1987-04-16 1988-11-03 Hoechst Ag Bifunktionelle proteine
DE3545568A1 (de) * 1985-12-21 1987-07-16 Hoechst Ag Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
DE3844211A1 (de) * 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
WO1991000912A1 (fr) * 1989-07-07 1991-01-24 Massachusetts Institute Of Technology Production et utilisation d'inhibiteurs de protease hybrides
CU22222A1 (es) * 1989-08-03 1995-01-31 Cigb Procedimiento para la expresion de proteinas heterologicas producidas de forma fusionada en escherichia coli, su uso, vectores de expresion y cepas recombinantes
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE3942580A1 (de) * 1989-12-22 1991-06-27 Basf Ag Verfahren zur herstellung von hirudin
US5270181A (en) * 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
ES2218622T3 (es) 1996-07-26 2004-11-16 Aventis Pharma Deutschland Gmbh Derivados de insulina con actividad de union al zinc incrementada.
DE19726167B4 (de) 1997-06-20 2008-01-24 Sanofi-Aventis Deutschland Gmbh Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
DE10033195A1 (de) * 2000-07-07 2002-03-21 Aventis Pharma Gmbh Bifunktionale Fusionsproteine aus Hirudin und TAP
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10114178A1 (de) 2001-03-23 2002-10-10 Aventis Pharma Gmbh Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität
DE10227232A1 (de) 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Saure Insulinzubereitungen mit verbesserter Stabilität
HUE037449T2 (hu) 2008-10-17 2018-08-28 Sanofi Aventis Deutschland Egy inzulin és egy GLP-1 agonista kombinációja
RU2537239C2 (ru) 2009-11-13 2014-12-27 Санофи-Авентис Дойчланд Гмбх Фармацевтическая композиция, включающая агонист glp-1, инсулин и метионин
PE20121316A1 (es) 2009-11-13 2012-10-05 Sanofi Aventis Deutschland Composicion farmaceutica que comprende un agonista de glp-1 y metionina
PT2611458T (pt) 2010-08-30 2016-12-16 Sanofi Aventis Deutschland Utilização de ave0010 para o fabrico de um medicamento para o tratamento da diabetes mellitus tipo 2
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
PT2750699E (pt) 2011-08-29 2015-11-03 Sanofi Aventis Deutschland Acelerómetro pendular
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
CA2970200A1 (fr) 2014-12-12 2016-06-16 Sanofi-Aventis Deutschland Gmbh Formulation contenant un rapport fixe d'insuline glargine/lixisenatide
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
KR20200080748A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
KR20200080747A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985000817A1 (fr) * 1983-08-10 1985-02-28 Amgen Expression microbienne de l'interleukine ii
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i
CA1341417C (fr) * 1984-03-27 2003-01-21 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
EP0158198A1 (fr) * 1984-03-29 1985-10-16 Takeda Chemical Industries, Ltd. ADN et son utilisation
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3526995A1 (de) * 1985-07-27 1987-02-05 Hoechst Ag Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
CA1297003C (fr) * 1985-09-20 1992-03-10 Jack H. Nunberg Composition et methode pour traiter les animaux

Also Published As

Publication number Publication date
DK52292A (da) 1992-04-21
DE3684892D1 (de) 1992-05-21
EP0468539A1 (fr) 1992-01-29
ES2077747T3 (es) 1995-12-01
IE59488B1 (en) 1994-03-09
AU6569386A (en) 1987-06-04
DE3541856A1 (de) 1987-06-04
ATE122397T1 (de) 1995-05-15
NO176481C (no) 1995-04-12
FI864798A (fi) 1987-05-28
DE3650322D1 (de) 1995-06-14
FI93471B (fi) 1994-12-30
DK172210B1 (da) 1998-01-05
FI864798A0 (fi) 1986-11-25
IL80755A0 (en) 1987-02-27
ZA868943B (en) 1987-07-29
DK568586D0 (da) 1986-11-26
CA1341203C (fr) 2001-03-13
NO176481B (no) 1995-01-02
ATE127841T1 (de) 1995-09-15
NO864759D0 (no) 1986-11-26
KR870005098A (ko) 1987-06-04
JP2566933B2 (ja) 1996-12-25
HU203579B (en) 1991-08-28
KR950000300B1 (ko) 1995-01-13
EP0227938A3 (en) 1988-11-23
ES2032378T3 (es) 1993-02-16
DE3650396D1 (de) 1995-10-19
FI93471C (fi) 1995-04-10
GR3005042T3 (fr) 1993-05-24
HUT43642A (en) 1987-11-30
DK52292D0 (da) 1992-04-21
EP0468539B1 (fr) 1995-09-13
DK568586A (da) 1987-05-28
EP0227938A2 (fr) 1987-07-08
PT83813A (de) 1986-12-01
JPS62143696A (ja) 1987-06-26
EP0464867A1 (fr) 1992-01-08
ES2073081T3 (es) 1995-08-01
EP0227938B1 (fr) 1992-04-15
EP0464867B1 (fr) 1995-05-10
IE863119L (en) 1987-05-27
AU595262B2 (en) 1990-03-29
PT83813B (pt) 1989-06-30

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Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired