DK1650307T3 - Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer - Google Patents

Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer

Info

Publication number
DK1650307T3
DK1650307T3 DK05020550.9T DK05020550T DK1650307T3 DK 1650307 T3 DK1650307 T3 DK 1650307T3 DK 05020550 T DK05020550 T DK 05020550T DK 1650307 T3 DK1650307 T3 DK 1650307T3
Authority
DK
Denmark
Prior art keywords
recombinant proteins
apoptosis inhibitors
preparation
apoptosis
inhibitors
Prior art date
Application number
DK05020550.9T
Other languages
English (en)
Inventor
De Goor Jana Van
Robert W Hamilton
Vishva Dixit
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22558684&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1650307(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DK1650307T3 publication Critical patent/DK1650307T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK05020550.9T 1999-09-27 2000-09-25 Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer DK1650307T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15623299P 1999-09-27 1999-09-27
EP00965426A EP1220934B1 (en) 1999-09-27 2000-09-25 Methods for making recombinant proteins using apoptosis inhibitors

Publications (1)

Publication Number Publication Date
DK1650307T3 true DK1650307T3 (da) 2010-10-04

Family

ID=22558684

Family Applications (2)

Application Number Title Priority Date Filing Date
DK00965426T DK1220934T3 (da) 1999-09-27 2000-09-25 Fremgangsmåde til fremstilling af rekombinante proteiner med anvendelse af apoptoseinhibitorer
DK05020550.9T DK1650307T3 (da) 1999-09-27 2000-09-25 Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK00965426T DK1220934T3 (da) 1999-09-27 2000-09-25 Fremgangsmåde til fremstilling af rekombinante proteiner med anvendelse af apoptoseinhibitorer

Country Status (13)

Country Link
US (4) US6586206B1 (da)
EP (2) EP1650307B1 (da)
JP (1) JP2003510087A (da)
AT (2) ATE313634T1 (da)
AU (1) AU7614600A (da)
CA (1) CA2384880C (da)
DE (2) DE60025015T2 (da)
DK (2) DK1220934T3 (da)
ES (2) ES2254230T3 (da)
HK (1) HK1091228A1 (da)
IL (2) IL148512A0 (da)
PT (1) PT1650307E (da)
WO (1) WO2001023592A2 (da)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE313634T1 (de) * 1999-09-27 2006-01-15 Genentech Inc Verfahren zur herstellung von rekombinanten proteinen mittels inhibitoren von apoptose
US7405038B1 (en) * 2000-03-03 2008-07-29 Boyce Thompson Institute For Plant Research, Inc. Stable cell lines resistant to apoptosis and nutrient stress and methods of making same
ATE443131T1 (de) * 2001-07-10 2009-10-15 Biogen Idec Inc Hemmung des apoptoseprozesses und verbesserung der zelleistung
KR100454016B1 (ko) * 2002-01-05 2004-10-26 한국과학기술원 항예정사 유전자로 형질전환되고 ｄｈｆｒ 유전자가결핍된 신규한 ｃｈｏ 세포주, 그의 제조 방법 및 상기형질전환된 ｃｈｏ 숙주 세포를 이용한 목적단백질의 생산방법
EP1676911B1 (en) * 2003-10-24 2010-04-21 Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute Novel recombinant animal cells with high protein production, method of constructing the same and method of mass protein production using the same
US7531327B2 (en) * 2004-07-23 2009-05-12 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US7537930B2 (en) * 2004-07-23 2009-05-26 Immunomedics, Inc. Mammalian cell lines for increasing longevity and protein yield from a cell culture
AU2005333513B2 (en) * 2004-07-23 2011-06-02 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
EP1802646B1 (en) * 2004-07-23 2017-09-06 Immunomedics Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US7608425B2 (en) * 2004-07-23 2009-10-27 Immunomedics, Inc. Methods for protein expression in mammalian cells in serum-free medium
CA2579677A1 (en) * 2004-09-16 2006-03-30 Sangamo Biosciences, Inc. Compositions and methods for protein production
KR20070082597A (ko) 2004-12-30 2007-08-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 유전자
US20060263809A1 (en) * 2005-04-05 2006-11-23 The Rgents Of The University Of California Method for sensitive measure of low level apoptosis in cells
US20070092947A1 (en) * 2005-10-20 2007-04-26 Immunomedics, Inc. Methods and compositions for increasing longevity and protein yield from a cell culture
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
US8273722B2 (en) * 2007-07-13 2012-09-25 Dharmacon, Inc. Enhanced biotherapeutic production using inhibitory RNA
WO2009048024A1 (ja) * 2007-10-09 2009-04-16 Hiroshima University 目的遺伝子を染色体外で高度に増幅させるためのベクターおよびその利用
CN101952303A (zh) * 2008-02-25 2011-01-19 诺维信公司 提高目标蛋白质的表达产率的方法
US20100049284A1 (en) * 2008-05-15 2010-02-25 The Catholic University Of America Use of heat to treat biological systems exposed to damaging radiation
CN102216459A (zh) 2008-09-15 2011-10-12 弗·哈夫曼-拉罗切有限公司 调节细胞摩尔渗透压浓度的组合物和方法
JP5808249B2 (ja) * 2008-10-20 2015-11-10 アッヴィ・インコーポレイテッド プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
CA2752729A1 (en) 2009-02-20 2010-08-26 Ventria Bioscience Cell culture media containing combinations of proteins
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
WO2014022102A1 (en) * 2012-08-01 2014-02-06 Amgen Inc. Methods of using anti-apoptotic compounds to modulate one or more properties of a cell culture
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
DE102013206438A1 (de) 2013-04-11 2014-10-16 Bühler Motor GmbH Permanentmagnetrotor für einen elektronisch kommutierten Gleichstrommotor
IL305656A (en) 2013-09-23 2023-11-01 Wilson Wolf Mfg Corporation Improved methods for genetic modification in animal cells
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
MX2017000231A (es) 2014-07-09 2017-06-27 Genentech Inc Ajuste del ph para mejorar la recuperacion por descongelamiento de bancos de celulas.
CN111349615A (zh) * 2018-12-24 2020-06-30 上海细胞治疗集团有限公司 制备过表达外源基因的细胞的方法
CN112080439B (zh) * 2020-07-30 2023-01-10 浙江工业大学 人源细胞凋亡调控蛋白Bcl-2在提高酿酒酵母橙花叔醇产量中的应用
EP4196598A2 (en) * 2020-08-14 2023-06-21 Bristol-Myers Squibb Company Method of making protein

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2971218B2 (ja) * 1991-12-04 1999-11-02 協和醗酵工業株式会社 ウリカーゼ遺伝子およびウリカーゼの製造法
GB9202796D0 (en) 1992-02-11 1992-03-25 Wellcome Found Antiviral antibody
JPH09163983A (ja) * 1995-08-30 1997-06-24 Hisamitsu Pharmaceut Co Inc アポトーシス抑制による有用物質の効率的生産方法および細胞
FR2749022B1 (fr) * 1996-05-23 2001-06-01 Rhone Merieux Cellules aviaires immortelles
FR2749021B1 (fr) * 1996-05-23 2001-10-26 Agronomique Inst Nat Rech Lignees de cellules aviaires immortalisees
US5876923A (en) * 1996-07-26 1999-03-02 Arch Development Corporation Herpes simplex virus ICP4 as an inhibitor of apoptosis
CA2265853C (en) * 1996-09-12 2010-08-03 Idun Pharmaceuticals, Inc. Inhibition of apoptosis using interleukin-1.beta.-converting enzyme (ice)/ced-3 family inhibitors
US6072047A (en) 1997-02-13 2000-06-06 Immunex Corporation Receptor that binds trail
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
DE60035515T2 (de) * 1999-09-08 2008-04-17 GeneTrol Biotherapeutics, Inc., Oakland Hohe zytokinproduktion mit verbesserter zelllebensfähigkeit
ATE313634T1 (de) * 1999-09-27 2006-01-15 Genentech Inc Verfahren zur herstellung von rekombinanten proteinen mittels inhibitoren von apoptose

Also Published As

Publication number Publication date
WO2001023592A2 (en) 2001-04-05
EP1650307B1 (en) 2010-07-14
EP1220934B1 (en) 2005-12-21
EP1220934A2 (en) 2002-07-10
DE60025015T2 (de) 2006-08-03
AU7614600A (en) 2001-04-30
US20100087624A1 (en) 2010-04-08
ATE474052T1 (de) 2010-07-15
PT1650307E (pt) 2010-10-21
ATE313634T1 (de) 2006-01-15
US20040002139A1 (en) 2004-01-01
CA2384880A1 (en) 2001-04-05
WO2001023592A3 (en) 2001-12-20
CA2384880C (en) 2010-09-21
HK1091228A1 (en) 2007-01-12
DE60044690D1 (de) 2010-08-26
DK1220934T3 (da) 2006-05-08
JP2003510087A (ja) 2003-03-18
EP1650307A3 (en) 2006-05-03
IL148512A0 (en) 2002-09-12
US20070004009A1 (en) 2007-01-04
DE60025015D1 (de) 2006-01-26
EP1650307A2 (en) 2006-04-26
US6586206B1 (en) 2003-07-01
IL148512A (en) 2011-04-28
ES2346659T3 (es) 2010-10-19
ES2254230T3 (es) 2006-06-16

Similar Documents

Publication Publication Date Title
DK1650307T3 (da) Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer
DE60216959D1 (de) Sich in schlagende kardiomyozyten verwandelnde stammzellen
DE60144145D1 (de) Subtilisin-variante
DK1226233T3 (da) Multipotente voksne stamceller og fremgangsmåder til isolering heraf
ATA165999A (de) Medium zur protein- und serumfreien kultivierung von zellen
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
MY125594A (en) Immortalized cell lines for virus growth
AU2002356749A1 (en) Process for the production of polypeptides in mammalian cell cultures
EP1483281A4 (en) METHOD FOR THE PRODUCTION OF INTERFERING RNA MOLECULES IN MAMMALIAN CELLS AND THERAPEUTIC APPLICATIONS OF SUCH MOLECULES
WO2002029083A3 (en) Industrial-scale serum-free production of recombinant proteins in mammalian cells
DK0918848T3 (da) Fremgangsmåder til at behandle kræftsygdomme og patogene infektioner ved anvendelse af antigenpræsenterende celler påfyldt med RNA
DK0904351T3 (da) Dyreceller og fremgangsmåder til replikationen af influenzavirus
ATE430807T1 (de) Verfahren zur kultivierung von circovirus
ATE342742T1 (de) Zusammensetzung zur geweberegeneration
FR2812004B1 (fr) Milieux de culture pour fecondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons
DK1131408T3 (da) Celler, dyrkningsmetoder og deres anvendelse ved autolog transplantationsbehandling
MXPA04001720A (es) Acido nucleicos bv8 y polipeptidos con actividad mitogenica.
DK1185621T3 (da) Overflade til cellekultur
GB2379446A (en) Cells,culture methods and their uses
DE69737597D1 (de) Vektoren zur inhibierung von viralem und tumorwachstum
DK1238057T3 (da) Fremgangsmåde til produktion af vedhæftende dyreceller
FI951285A0 (fi) Pilokarpiinin valmistusmenetelmä
ITMI991408A0 (it) Processo per la produzione di virus in colture cellulari