DK1572994T3 - Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer - Google Patents

Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer

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Publication number
DK1572994T3
DK1572994T3 DK03782989T DK03782989T DK1572994T3 DK 1572994 T3 DK1572994 T3 DK 1572994T3 DK 03782989 T DK03782989 T DK 03782989T DK 03782989 T DK03782989 T DK 03782989T DK 1572994 T3 DK1572994 T3 DK 1572994T3
Authority
DK
Denmark
Prior art keywords
chromatin
openers
methods
nucleic acid
protein
Prior art date
Application number
DK03782989T
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Danish (da)
English (en)
Inventor
Theodorus Hendrikus Jaco Kwaks
Arie Pieter Otte
Richard George Antonius Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of DK1572994T3 publication Critical patent/DK1572994T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
DK03782989T 2002-12-20 2003-12-19 Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer DK1572994T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02080479 2002-12-20
EP03075089 2003-01-13
PCT/NL2003/000909 WO2004056986A2 (fr) 2002-12-20 2003-12-19 Moyens et methodes de production d'une proteine a l'aide de systemes d'ouverture de la chromatine permettant de rendre la chromatine plus accessible aux facteurs de transcription

Publications (1)

Publication Number Publication Date
DK1572994T3 true DK1572994T3 (da) 2007-05-29

Family

ID=32683814

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03782989T DK1572994T3 (da) 2002-12-20 2003-12-19 Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer

Country Status (7)

Country Link
US (1) US8263393B2 (fr)
EP (1) EP1572994B1 (fr)
AT (1) ATE354645T1 (fr)
AU (1) AU2003290453A1 (fr)
DE (1) DE60312039T2 (fr)
DK (1) DK1572994T3 (fr)
WO (1) WO2004056986A2 (fr)

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DK1806407T3 (da) 2001-07-04 2010-08-16 Chromagenics Bv DNA-sekvenser med anti-repressoraktivitet
EP1513936A2 (fr) 2002-06-14 2005-03-16 Chromagenics B.V. Utilisation de sequences de blocage de repression pour ameliorer l'expression d e genes
CA2722998C (fr) 2002-06-14 2014-12-02 Chromagenics B.V. Procede permettant la production simultanee de plusieurs proteines; vecteurs et cellules utilises dans ce procede
ES2373549T3 (es) 2002-12-18 2012-02-06 Chromagenics B.V. Método para mejorar la producción de proteínas.
DE60312039T2 (de) 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US7825232B2 (en) * 2004-07-08 2010-11-02 Chromagenics B.V. Sequence for improving expression of nucleic acid
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
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SI1809750T1 (sl) * 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
ES2687447T3 (es) * 2008-11-28 2018-10-25 Anton Bauer Vector cromosómico artificial
US8476019B2 (en) 2009-10-30 2013-07-02 Synaptic Research, Llc Enhanced gene expression in algae
JP6309461B2 (ja) * 2012-02-28 2018-04-11 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．， ＬＬＣ ヒストンアセチル化の標的化
BR112016025972B1 (pt) * 2014-05-19 2022-04-19 F. Hoffmann-La Roche Ag Métodos para selecionar um clone celular/linhagem celular e método para a produção de um polipeptídeo
US20230233607A1 (en) * 2020-02-17 2023-07-27 The Regents Of The University Of California Light-inducible gene activation systems and methods for making and using them

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Also Published As

Publication number Publication date
EP1572994A2 (fr) 2005-09-14
WO2004056986A2 (fr) 2004-07-08
US8263393B2 (en) 2012-09-11
AU2003290453A8 (en) 2004-07-14
DE60312039T2 (de) 2007-11-08
AU2003290453A1 (en) 2004-07-14
ATE354645T1 (de) 2007-03-15
WO2004056986A3 (fr) 2004-08-19
US20060003416A1 (en) 2006-01-05
EP1572994B1 (fr) 2007-02-21
DE60312039D1 (de) 2007-04-05

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