DK1476562T3 - Overekspression af extremozymgener i pseudomonas og nært beslægtede bakterier - Google Patents

Overekspression af extremozymgener i pseudomonas og nært beslægtede bakterier

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Publication number
DK1476562T3
DK1476562T3 DK03711011.1T DK03711011T DK1476562T3 DK 1476562 T3 DK1476562 T3 DK 1476562T3 DK 03711011 T DK03711011 T DK 03711011T DK 1476562 T3 DK1476562 T3 DK 1476562T3
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DK
Denmark
Prior art keywords
closely related
overexpression
pseudomonas
related bacteria
extremozyme
Prior art date
Application number
DK03711011.1T
Other languages
English (en)
Inventor
Lawrence C Chew
Henry W Talbot
Stacey L Lee
Original Assignee
Dow Global Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc filed Critical Dow Global Technologies Inc
Application granted granted Critical
Publication of DK1476562T3 publication Critical patent/DK1476562T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03711011.1T 2002-02-13 2003-02-13 Overekspression af extremozymgener i pseudomonas og nært beslægtede bakterier DK1476562T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2002/004294 WO2003068948A1 (en) 2002-02-13 2002-02-13 Over-expression of extremozyme genes in pseudomonads and closely related bacteria
PCT/US2003/004288 WO2003068926A2 (en) 2002-02-13 2003-02-13 Over-expression of extremozyme genes in pseudomonads and closely related bacteria

Publications (1)

Publication Number Publication Date
DK1476562T3 true DK1476562T3 (da) 2010-04-19

Family

ID=27732085

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03711011.1T DK1476562T3 (da) 2002-02-13 2003-02-13 Overekspression af extremozymgener i pseudomonas og nært beslægtede bakterier

Country Status (14)

Country Link
US (1) US20050130160A1 (da)
EP (1) EP1476562B1 (da)
JP (1) JP2006501811A (da)
CN (1) CN100451102C (da)
AT (1) ATE454463T1 (da)
AU (2) AU2002306484A1 (da)
BR (1) BR0307630B1 (da)
CA (1) CA2475926A1 (da)
DE (1) DE60330841D1 (da)
DK (1) DK1476562T3 (da)
MX (1) MXPA04007886A (da)
PL (1) PL372107A1 (da)
RU (1) RU2004127236A (da)
WO (2) WO2003068948A1 (da)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003089455A2 (en) 2002-04-22 2003-10-30 Dow Global Technologies Inc. Low-cost production of peptides
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
JP5087741B2 (ja) * 2003-11-19 2012-12-05 フェネックス インコーポレイテッド 改良タンパク質発現系
EP1687324B1 (en) 2003-11-21 2012-08-22 Pfenex, Inc. Improved expression systems with sec-system secretion
ES2707786T3 (es) 2004-01-16 2019-04-05 Pfenex Inc Expresión de proteínas de mamífero en Pseudomonas fluorescens
JP2007528727A (ja) 2004-02-27 2007-10-18 ザ ダウ ケミカル カンパニー 植物細胞における高効率ペプチド生産
EP1774017B1 (en) 2004-07-26 2013-05-15 Pfenex Inc. Process for improved protein expression by strain engineering
US7727755B2 (en) * 2005-11-02 2010-06-01 Battelle Energy Alliance, Llc Enzyme and methodology for the treatment of a biomass
US8969033B2 (en) 2005-11-02 2015-03-03 Battelle Energy Alliance, Llc Alteration and modulation of protein activity by varying post-translational modification
JP5714230B2 (ja) 2007-01-31 2015-05-07 フェネックス インコーポレイテッド 発現上昇のための細菌リーダー配列
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
MX2010003600A (es) 2007-10-03 2010-12-14 Verenium Corp Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
WO2009076196A1 (en) 2007-12-07 2009-06-18 Dow Global Technologies Inc. High copy number self-replicating plasmids in pseudomonas
US9732330B2 (en) 2008-01-25 2017-08-15 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
US8492114B2 (en) 2008-01-25 2013-07-23 Battelle Energy Alliance, Llc Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
US8728803B2 (en) 2008-02-28 2014-05-20 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic metabolism genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
EP2242841A4 (en) 2008-01-25 2011-05-18 Battelle Energy Alliance Llc BETA-XYLOSIDASES TOLERANT TO ACID AND HEAT, GENES ENCODING THESE BETA-XYLOSIDASES, ASSOCIATED ORGANISMS AND METHODS
US8557557B2 (en) 2008-01-31 2013-10-15 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
US8497110B2 (en) 2008-01-31 2013-07-30 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
WO2009099858A1 (en) 2008-01-31 2009-08-13 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer- degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
US8426185B2 (en) 2008-01-31 2013-04-23 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
MX2010008575A (es) 2008-02-22 2010-12-07 Battelle Energy Alliance Llc Control transcripcional en alicyclobacillus acidocaldarius y genes, proteinas y metodos asociados.
WO2009145944A2 (en) 2008-02-26 2009-12-03 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
AU2009310370A1 (en) 2008-02-27 2010-05-06 Battelle Energy Alliance, Llc Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
CN101613681B (zh) * 2008-06-24 2013-07-10 中国科学院上海生命科学研究院 重组极端耐热α-淀粉酶的复性与纯化方法
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) * 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010132079A1 (en) * 2009-05-15 2010-11-18 University Of Florida Research Foundation, Inc. Increased expression of transhydrogenase genes and use thereof in ethanol production
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
US20110275135A1 (en) 2010-05-05 2011-11-10 Battelle Energy Alliance, Llc Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles
WO2013148163A1 (en) 2012-03-30 2013-10-03 Verenium Corporation Genes encoding cellulase
GB201308853D0 (en) 2013-03-12 2013-07-03 Verenium Corp Genes encoding xylanase
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
JP2016525354A (ja) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー フィターゼ
WO2019063613A1 (en) 2017-09-26 2019-04-04 Dsm Ip Assets B.V. ENZYMATIC ELIMINATION OF CHLOROPHYLL SUBSTRATES FROM TRIACYLGLYCEROL OILS
US11098265B2 (en) 2019-03-27 2021-08-24 Bunge Global Innovation, Llc Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4753876A (en) 1984-03-21 1988-06-28 Monsanto Company Marker genes in pseudomonad bacteria
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
US5028530A (en) 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US6168945B1 (en) * 1988-03-23 2001-01-02 The Board Of Regents Of The University Of Oklahoma Genes encoding operon and promoter for branched chain keto acid dehydrogenase of pseudomonas putida and methods
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5356796A (en) 1990-03-30 1994-10-18 The University Of Alaska Repressor protein and operon for regulating expression of polypeptides and its use in the preparation of 2,2-dialkyglycine decarboxylase of Pseudomonas cepacia
US5210025A (en) 1990-03-30 1993-05-11 University Of Alaska Prepressor protein gene for regulating expression of polypeptides and its use in the preparation of 2,2-dialkylglycine decarboxylase of Pseudomonas cepacia
US5670350A (en) 1990-08-20 1997-09-23 Novartis Finance Corporation Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression
JPH074256B2 (ja) * 1990-10-31 1995-01-25 栗田工業株式会社 リパーゼの活性発現を調節する遺伝子、ベクターおよびリパーゼの生産方法
US5578479A (en) * 1992-06-09 1996-11-26 The Johns Hopkins University Alpha-amylase from hyperthermophilic archaebacterium
US5366883A (en) 1992-06-09 1994-11-22 Takara Shuzo Co., Ltd. α-amylase gene
EP0648843A1 (en) 1993-10-01 1995-04-19 Takara Shuzo Co. Ltd. DNA encoding a hyperthermostable alpha-amylase
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
GB2291058B (en) 1994-07-14 1998-12-23 Solvay Acid-stable and thermo-stable alpha-amylases derived from sufolobus species
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
WO1998045417A1 (en) 1997-04-09 1998-10-15 Michigan State University HYPERTHERMOSTABLE α-AMYLASE
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US6261842B1 (en) 1997-10-23 2001-07-17 Wisconsin Alumni Research Foundation Microorganism genomics, compositions and methods related thereto
US6090593A (en) 1998-05-13 2000-07-18 The United States Of America As Represented By The Secretary Of The Air Force Isolation of expressed genes in microorganisms
KR100312456B1 (ko) * 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
JP2000350585A (ja) * 1999-06-10 2000-12-19 Tokyo Gas Co Ltd 耐熱耐酸素性ヒドロゲナーゼ遺伝子
CA2360750A1 (en) * 1999-11-22 2001-05-31 Diversa Corporation Capillary array-based sample screening
US20010047029A1 (en) 2000-04-26 2001-11-29 Handelsman Jo E. Triaryl cation antibiotics from environmental DNA
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens

Also Published As

Publication number Publication date
DE60330841D1 (de) 2010-02-25
RU2004127236A (ru) 2005-04-20
EP1476562A2 (en) 2004-11-17
WO2003068926A3 (en) 2004-02-26
JP2006501811A (ja) 2006-01-19
WO2003068948A1 (en) 2003-08-21
ATE454463T1 (de) 2010-01-15
BR0307630A (pt) 2005-01-11
AU2003215197B2 (en) 2008-06-26
PL372107A1 (en) 2005-07-11
CN1646694A (zh) 2005-07-27
EP1476562A4 (en) 2005-12-07
CA2475926A1 (en) 2003-08-21
WO2003068926A2 (en) 2003-08-21
AU2002306484A1 (en) 2003-09-04
AU2003215197A1 (en) 2003-09-04
EP1476562B1 (en) 2010-01-06
BR0307630B1 (pt) 2014-08-19
CN100451102C (zh) 2009-01-14
MXPA04007886A (es) 2004-10-15
US20050130160A1 (en) 2005-06-16

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