DK1356037T3 - Bibliotek med en samling af celler - Google Patents

Bibliotek med en samling af celler

Info

Publication number
DK1356037T3
DK1356037T3 DK02709979.5T DK02709979T DK1356037T3 DK 1356037 T3 DK1356037 T3 DK 1356037T3 DK 02709979 T DK02709979 T DK 02709979T DK 1356037 T3 DK1356037 T3 DK 1356037T3
Authority
DK
Denmark
Prior art keywords
novel
cells
libraries
host cells
library
Prior art date
Application number
DK02709979.5T
Other languages
Danish (da)
English (en)
Inventor
Neil Goldsmith
Alexandra M P Sorensen
Soren V S Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of DK1356037T3 publication Critical patent/DK1356037T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Eye Examination Apparatus (AREA)
  • Holo Graphy (AREA)
  • Aerials With Secondary Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK02709979.5T 2001-01-25 2002-01-25 Bibliotek med en samling af celler DK1356037T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100128 2001-01-25
DKPA200100679 2001-05-01
US30086301P 2001-06-27 2001-06-27
PCT/DK2002/000056 WO2002059297A2 (en) 2001-01-25 2002-01-25 A library of a collection of cells

Publications (1)

Publication Number Publication Date
DK1356037T3 true DK1356037T3 (da) 2011-06-27

Family

ID=43646216

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02709979.5T DK1356037T3 (da) 2001-01-25 2002-01-25 Bibliotek med en samling af celler

Country Status (9)

Country Link
US (2) US7838287B2 (https=)
EP (1) EP1356037B1 (https=)
JP (2) JP2004525624A (https=)
AT (1) ATE501251T1 (https=)
AU (1) AU2002227883A1 (https=)
CA (1) CA2474172C (https=)
DE (1) DE60239386D1 (https=)
DK (1) DK1356037T3 (https=)
WO (1) WO2002059297A2 (https=)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
EP1472349B1 (en) * 2002-01-25 2008-10-29 Evolva AG Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
DK2314629T4 (da) 2002-07-18 2023-02-06 Merus Nv Rekombinant produktion af blandinger af antistoffer
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
WO2004016791A1 (en) * 2002-08-01 2004-02-26 Evolva Ltd Methods of mixing large numbers of heterologous genes
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP1737971B1 (en) * 2004-01-20 2017-08-16 Merus N.V. Mixtures of binding proteins
CN103589650A (zh) 2005-03-18 2014-02-19 米克罗比亚公司 产油酵母和真菌中类胡萝卜素的产生
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
CN102282155B (zh) 2008-12-02 2017-06-09 日本波涛生命科学公司 磷原子修饰的核酸的合成方法
KR101153866B1 (ko) 2009-05-21 2012-06-27 대한민국 형질전환용 운반체, 이의 제조방법, 이를 이용한 형질전환방법 및 형질전환체
RU2612521C2 (ru) 2009-07-06 2017-03-09 Онтории, Инк. Новые пролекарства нуклеиновых кислот и способы их применения
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
JP5868324B2 (ja) 2010-09-24 2016-02-24 株式会社Wave Life Sciences Japan 不斉補助基
CN103796657B (zh) 2011-07-19 2017-07-11 波涛生命科学有限公司 合成官能化核酸的方法
CN108396044A (zh) 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
US9248181B2 (en) 2012-04-20 2016-02-02 Merus B.V. Methods and means for the production of Ig-like molecules
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
KR102213609B1 (ko) 2012-07-13 2021-02-08 웨이브 라이프 사이언시스 리미티드 키랄 제어
CA2879066C (en) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
US20150293076A1 (en) * 2012-10-22 2015-10-15 Bolt Threads, Inc. Cellular Reprogramming for Product Optimization
SG11201505597SA (en) 2013-02-06 2015-08-28 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
CA2900882A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
CA2924343C (en) 2013-09-17 2025-09-16 Bolt Threads, Inc. PROCESSES AND COMPOSITIONS FOR SYNTHESIZING IMPROVED SILK FIBERS
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
BR112016016400A2 (pt) 2014-01-16 2017-10-03 Wave Life Sciences Ltd Composições de oligonucleotídeos quiralmente controlados, seu uso, sua composição farmacêutica, e métodos
AU2015303294A1 (en) 2014-08-11 2017-02-02 Evolva Sa. Production of steviol glycosides in recombinant hosts
AU2015314251A1 (en) 2014-09-09 2017-03-16 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
KR20190046993A (ko) 2016-09-14 2019-05-07 볼트 쓰레즈, 인크. 긴 균일한 재조합 단백질 섬유
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
JP2025170455A (ja) * 2022-10-05 2025-11-19 株式会社Logomix 細胞ライブラリおよびその製造方法
US20240209383A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
AU3556789A (en) 1988-04-22 1989-11-24 Massachusetts Institute Of Technology Process for forming and using microdroplets
WO1991000920A2 (en) 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
US5316922A (en) * 1992-04-15 1994-05-31 The United States Of America As Represented By Department Of Health And Human Services Method for indentifying and expressing proteins that recognize and adhere to specific probes
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
CA2174354C (en) 1993-10-27 2011-03-29 Paul B. Fisher Compositions of melanoma differentiation associated gene-7 (mda-7) in cancer diagnosis and treatment
US6268140B1 (en) * 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) * 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6004788A (en) * 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US20010041333A1 (en) * 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
GB9603803D0 (en) * 1996-02-22 1996-04-24 Zeneca Ltd Production of proteins, plasmids coding therefor and organisms containing such plasmids
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
CA2259158A1 (en) 1996-07-09 1998-01-15 The Government Of The United States Of America As Represented By The Sec Retary, Department Of Health And Human Services Transformation-associated recombination cloning
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
CA2282658C (en) 1997-02-27 2003-02-25 Cellomics, Inc. A system for cell-based screening
AU6571598A (en) 1997-03-18 1998-10-12 Chromaxome Corporation Methods for screening compounds using encapsulated cells
DE69732378D1 (de) 1997-05-27 2005-03-03 Hanil Synthetic Fiber Co Ltd Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae
GB9727512D0 (en) 1997-12-31 1998-02-25 Adprotech Plc Fuzzy genes and their application in molecular adjuvants
CA2321930A1 (en) 1998-03-04 1999-09-10 Diversa Corporation Production and use of normalized dna libraries
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
WO2000012680A1 (en) 1998-08-31 2000-03-09 Maxygen, Inc. Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes
EP1114320A2 (en) 1998-09-18 2001-07-11 Cellomics, Inc. A system for cell-based screening
AU3879300A (en) 1999-03-09 2000-09-28 Diversa Corporation End selection in directed evolution
AU4039400A (en) 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
GB9908814D0 (en) * 1999-04-16 1999-06-09 Celltech Therapeutics Ltd Process
AU7646000A (en) 1999-11-05 2001-05-14 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
US20010049104A1 (en) * 2000-03-24 2001-12-06 Stemmer Willem P.C. Methods for modulating cellular and organismal phenotypes
CA2474161C (en) 2001-01-25 2012-03-27 Evolva Ltd. Concatemers of differentially expressed multiple genes
WO2002059290A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A method for evolving a cell having desired phenotype and envolved cells
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
WO2002059330A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1472349B1 (en) * 2002-01-25 2008-10-29 Evolva AG Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
DE60319259T2 (de) 2002-06-14 2009-03-05 Dyax Corp., Cambridge Rekombination von nukleinsäurebibliotheksmitgliedern
US20030235823A1 (en) * 2002-06-24 2003-12-25 The University Of Alabama Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation
WO2004016791A1 (en) 2002-08-01 2004-02-26 Evolva Ltd Methods of mixing large numbers of heterologous genes

Also Published As

Publication number Publication date
AU2002227883A1 (en) 2002-08-06
WO2002059297A3 (en) 2003-04-10
ATE501251T1 (de) 2011-03-15
JP4965625B2 (ja) 2012-07-04
CA2474172A1 (en) 2002-08-01
JP2004525624A (ja) 2004-08-26
US7838287B2 (en) 2010-11-23
DE60239386D1 (de) 2011-04-21
US20040241672A1 (en) 2004-12-02
EP1356037A2 (en) 2003-10-29
WO2002059297A8 (en) 2004-04-15
JP2010043108A (ja) 2010-02-25
US20120094870A1 (en) 2012-04-19
WO2002059297A2 (en) 2002-08-01
CA2474172C (en) 2012-07-10
EP1356037B1 (en) 2011-03-09

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